This USGS data release presents tabular data of daily and annual (for the 9-month period March 1 through November 30) estimates of concentrations and fluxes of total and dissolved phosphorus, total nitrogen, chloride, and total suspended solids for 18 monitored tributaries of Lake Champlain. Daily estimates generally cover the period March 1990 through November 2014 and annual estimates 1990 through 2014, although dates vary by constituent and tributary, depending on availability of raw data. The dataset consists of 6 items: 1. Annual (9-month) estimates of concentration and flux (tabular dataset); 2. Daily estimates of concentration and flux of total phosphorus (tabular dataset); 3. Daily estimates of concentration and flux of dissolved phosphorus (tabular dataset); 4. Daily estimates of concentration and flux of total nitrogen (tabular dataset); 5. Daily estimates of concentration and flux of chloride (tabular dataset); 6. Daily estimates of concentration and flux of total suspended solids (tabular dataset). These data support the following publication: Medalie, L., 2016, Concentration, Flux, and Trend Estimates with Uncertainty for Nutrients, Chloride, and Total Suspended Solids in Tributaries of Lake Champlain, 1990–2014: U.S. Geological Survey Open-File Report 2016–1200, 24 p., https://dx.doi.org/10.3133/ofr20161200.