These data present chemistry and toxicity results from freshwater stream sediments collected from 99 wadable stream sites across eleven states in the Midwestern U.S. as one component of a larger USGS study in the summer of 2013. This data presents a selected suite of chemistry collected at these sites (PAHs, Organochlorines, PCBs, Trace Elements, and current use pesticides) used in calculating a Probable Effect Concentration-Likely Effect Benchmark quotient mixture score for contaminants measured in sediments. The toxicity data presents results of toxicity tests following ASTM and US EPA standard methods for sediment toxicity tests with the amphipod Hyalella azteca (28-d exposure), the midge Chironomus dilutus (10-d), and at fewer sites, with the freshwater mussel Lampsilis siliquoidea (28-d).