The Caloosahatchee River is located in Southwest Florida and drains northern parts of the Florida Everglades. It stretches 110 km (68 miles) inland and empties into the Gulf of Mexico at Ft. Myers and Cape Coral, FL. The lower section of the river is part of the Estero Bay Estuary system providing critical habitat for a large variety of plants and animals. The river has been greatly altered for navigation, agricultural and human development needs and its flow is managed by a series of upland locks and dams. The USGS, in cooperation with the SFWMD, performed a bathymetric survey of the lower Caloosahatchee River using a single beam hydrographic system. High resolution, acoustic bathymetric surveying is a proven method to map sea and river floor elevations. Survey track-lines were spaced 500-meter apart orientated along long axis of the river. Track-lines collected in across the river (intersecting track-lines) functioned to serve as a cross-check and to assess the relative vertical accuracy of the survey. Several perimeter survey lines were also collected. Soundings were collected along each track line at 3-meter spacing. In shallow areas, data was collected in a minimum of 0.6 meters water depth except where there is potential damage to the bottom environment or the boat/motors. This report serves as an archive of processed single-beam bathymetry data that were collected in the Caloosahatchee River, Florida in 2002. Geographic information system data products include a XYZ data, bathymetric contours, and USGS quadrangle map. Additional files include formal Federal Geographic Data Committee (FGDC) metadata.