<?xml version="1.0" encoding="UTF-8"?>
<metadata>
 <idinfo>
  <citation>
   <citeinfo>
    <origin>Lalk, Ellen</origin>
    <origin>Pohlman, John W.</origin>
    <origin>Lapham, Laura L.</origin>
    <origin>Casso, Michael</origin>
    <origin>Glenna, Madeline</origin>
    <origin>Hildebrand, Anna</origin>
    <origin>Lloyd, Karen</origin>
    <origin>Ma, Kun</origin>
    <origin>Seewald, Jeffrey</origin>
    <origin>Stock, Lennart</origin>
    <origin>Strauss, Maureen A.</origin>
    <origin>Sylva, Sean</origin>
    <origin>Traver, Lily</origin>
    <origin>Wilson, Rachel</origin>
    <origin>Williams, Leketha</origin>
    <pubdate>20250521</pubdate>
    <title>Porewater dissolved organic carbon and associated geochemical data for methane seeps in the Cascadia Margin: Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent</title>
    <edition>1.0</edition>
    <serinfo>
     <sername>data release</sername>
     <issue>DOI:10.26008/1912/bco-dmo.959765.1</issue>
    </serinfo>
    <pubinfo>
     <pubplace>Woods Hole Coastal and Marine Science Center, Woods Hole, Massachusetts, USA</pubplace>
     <publish>Biological and Chemical Oceanography Data Management Office (BCO-DMO)</publish>
    </pubinfo>
    <othercit>Suggested citation: Lalk, E., Pohlman, J.W., Lapham, L.L., Casso, M., Glenna, M., Hildebrand, A., Lloyd, K., Ma, K., Seewald, J., Stock, L., Strauss, M.A., Sylva, S., Traver, L., Wilson, R., Williams, L., 2025, Porewater dissolved organic carbon and associated geochemical data for methane seeps in the Cascadia Margin—Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent : Biological and Chemical Oceanography Data Management Office (BCO-DMO), https://doi.org/10.26008/1912/bco-dmo.959765.1 .</othercit>
    <onlink>https://doi.org/10.26008/1912/bco-dmo.959765.1</onlink>
   </citeinfo>
  </citation>
  <descript>
   <abstract>These data include vertical profiles of concentrations and stable carbon isotope values of dissolved organic carbon (DOC), dissolved inorganic carbon (DIC), total organic carbon (TOC) from sediment cores collected at methane seeps and reference sites. Sediment porosity and dissolved sulfate and methane concentrations are also presented. Methane seeps in Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent, along the Cascadia Margin, were sampled. These data were collected over two decades and across four cruises: PGC02-08 (CCGS John P. Tully, 2002), Hydrates 2004 Sea Trial (CCGS John P. Tully, 2004), AT50-14 (R/V Atlantis, 2023),  and AT50-29B (R/V Atlantis, 2024).</abstract>
   <purpose>These data are used to assess the prevalence of methane-derived DOC at methane seeps in the ocean, which may contribute ‘old’ carbon to the deep ocean or serve as a source of reduced carbon for the deep ocean microbial loop.</purpose>
   <supplinf>This Microsoft Excel workbook contains data in a single tab.
The cruises involved in this work are: R/V Atlantis - AT50-14, AT50-29B; Canadian Coast Guard Ship (CCGS) John P. Tully - PGC02-08 and Hydrates2024.</supplinf>
  </descript>
  <timeperd>
   <timeinfo>
    <rngdates>
     <begdate>2002</begdate>
     <enddate>2024</enddate>
    </rngdates>
   </timeinfo>
   <current>Ground condition. Cores were collected in 2002 using a piston corer, in 2004 using an ROV, and in 2023 and 2024 using a submersible. Pore waters were extracted from these cores during the cruise on which they were acquired. The measurements presented here were completed within 1 year post core collection for each cruise. Initial sample collection dates are listed as the parameter 'SamplingDate'.</current>
  </timeperd>
  <status>
   <progress>Complete</progress>
   <update>None planned</update>
  </status>
  <spdom>
   <bounding>
    <westbc>-126.849944</westbc>
    <eastbc>-124.6027</eastbc>
    <northbc>48.671875</northbc>
    <southbc>44.568359</southbc>
   </bounding>
  </spdom>
  <keywords>
   <theme>
    <themekt>None</themekt>
    <themekey>U.S. Geological Survey</themekey>
    <themekey>USGS</themekey>
    <themekey>Coastal and Marine Hazards and Resources Program</themekey>
    <themekey>CMHRP</themekey>
    <themekey>Woods Hole Coastal and Marine Science Center</themekey>
    <themekey>WHCMSC</themekey>
    <themekey>U.S. Department of Energy</themekey>
    <themekey>DOE</themekey>
    <themekey>National Energy Technology Laboratory</themekey>
    <themekey>NETL</themekey>
    <themekey>National Science Foundation</themekey>
    <themekey>NSF</themekey>
    <themekey>dissolved organic carbon</themekey>
    <themekey>methane seeps</themekey>
    <themekey>carbon cycle</themekey>
    <themekey>deep-sea</themekey>
    <themekey>carbon isotopes</themekey>
   </theme>
   <theme>
    <themekt>USGS Thesaurus</themekt>
    <themekey>carbon isotope analysis</themekey>
    <themekey>dissolved organic compounds</themekey>
    <themekey>interagency programs</themekey>
    <themekey>carbon cycling</themekey>
   </theme>
   <theme>
    <themekt>ISO 19115 Topic Category</themekt>
    <themekey>geoscientificInformation</themekey>
   </theme>
   <theme>
    <themekt>USGS Metadata Identifier</themekt>
    <themekey>USGS:fced8c1a-1e0c-4a7a-8caf-b5aa7cd072ab</themekey>
   </theme>
   <place>
    <placekt>Geographic Names Information System (GNIS)</placekt>
    <placekey>Pacific Ocean</placekey>
   </place>
  </keywords>
  <accconst>None</accconst>
  <useconst>Public domain (CC0-1.0) data from the U.S. Government are freely redistributable with proper metadata and source attribution. Please recognize the U.S. Geological Survey (USGS) as the source of this information.</useconst>
  <ptcontac>
   <cntinfo>
    <cntperp>
     <cntper>Ellen Lalk</cntper>
     <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
    </cntperp>
    <cntpos>Research Chemist</cntpos>
    <cntaddr>
     <addrtype>mailing and physical address</addrtype>
     <address>384 Woods Hole Rd.</address>
     <city>Woods Hole</city>
     <state>MA</state>
     <postal>02543-1598</postal>
    </cntaddr>
    <cntvoice>508-548-8700</cntvoice>
    <cntemail>elalk@usgs.gov</cntemail>
   </cntinfo>
  </ptcontac>
 </idinfo>
 <dataqual>
  <logic>For each of the chemical and isotopic property measurements described in the Process Steps below, standards relevant to the particular property were entrained in the measurement sequence to ensure fidelity within each Process Step. The standards are noted in the Process Steps as well as the citations for those references.</logic>
  <complete>This Microsoft Excel workbook represents the complete collected and calculated data in support of the Cascadia Margin dissolved organic carbon study.</complete>
  <posacc>
   <horizpa>
    <horizpar>For the 2002 cruise, horizontal positioning information for cores was achieved with an acoustic bottom transponder constellation, which provided an accuracy of 1-2 meters.

For the 2004 cruise, positioning information for the underwater vehicle ROPOS (Remotely Operated Platform for Ocean Science) relied on Long Baseline (LBL) transponder technology. Ideally, this system provides sub-meter location accuracy (as noted by a commercial producer of the technology: https://www.hydro-international.com/content/article/lbl-underwater-positioning). ROPOS cruise reports from the early 2000s (https://www.pmel.noaa.gov/eoi/nemo2006/nemo06-crrpt-final.pdf) note the positional accuracy degraded on several occasions, with the worst reported accuracy (50 - 100 m) providing an upper bound on the positional accuracy for the 2004 samples.

Samples collected in 2023 and 2024 for this study utilized underwater vehicles launched from the R/V Atlantis. The R/V Atlantis vehicles use a Sonardyne Ranger 2 ultra-short baseline (USBL) positioning system. Horizontal position for this system was found to be ~1% of the depth, meaning the horizontal position accuracy for this study is 7-13 meters.  USBL accuracy study found on slide 17 at https://www.unols.org/sites/default/files/201910rvt_breakout03_shipboard_USBL_systems.pdf</horizpar>
   </horizpa>
   <vertacc>
    <vertaccr>Material collected for this study was subsampled from shallow cores (within 9 meters of the sediment surface). Core locations were determined based on standard tape-measure core layouts, with 0.25 cm accuracy.

Water depth is available via tidally-corrected shipboard bathymetric measurements with an accuracy of 0.3% of water depth (https://mac.unols.org/wp-content/uploads/2021/10/Atlantis_2021_EM124_SAT_report_v1p0.pdf). The seafloor sediment collection platforms are also equipped with pressure-based depth sensors accuracy to 0.1% of water depth (https://www.researchgate.net/publication/275271368). An upper bound on the water depth accuracy is taken here as the less accurate shipboard measurement of 0.3% of water depth.

For the spatial reference information, the seabed surface reference is used because the sediment-water interface is the most relevant physical boundary associated with these data.</vertaccr>
   </vertacc>
  </posacc>
  <lineage>
   <procstep>
    <procdesc>Sediment and porewater handling; PCG02-08- Core material was sectioned and pressure-squeezed with a modified Reeburgh squeezer (Reeburgh, 1967) into acid-washed all-plastic syringes. Porewater was then filtered through 0.45 um polyethersulphone syringe filters. Hydrates2004- Core material was sectioned and pressure-squeezed with a modified Reeburgh squeezer (Reeburgh, 1967) into acid-washed all-plastic syringes. Porewater was then filtered through 0.2 um arodisc polyethersulphone syringe filters. AT50-14- Core material was sectioned and pressure-squeezed with a modified Reeburgh squeezer (Reeburgh, 1967) into acid-washed all-plastic syringes. Porewater was then filtered through pre-combusted 0.4 um glass fiber filters. AT50-29B- Core material was sectioned into acid-washed falcon tubes and refrigerated. Porewater was extracted using Rhizons with a 0.2 um filter size. The process step took place over a time period of 2002 to 2024. The cores were collected and porewater was squeezed during field expeditions in 2002, 2004, 2023, and 2024. The process date represents the most recent completion of the work.

Reference:

Reeburgh, W.S., 1967, An improved interstitial water sampler: Limnology and Oceanography, 846 12(1), 163–165, https://doi.org/10.4319/lo.1967.12.1.0163</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>Porosity; Fresh sediment was placed in pre-weighed petri-dishes, sealed, and refrigerated until measurement in lab. Petri dishes were weighed upon return to shore, then were played in a drying oven (45 degress Celsius, 1 week). Petri dishes with dried sediment were weighed again. The difference between the wet and dry measurements is the water weight, and the difference between the dry weight and the petri dish is the sediment weight. Assuming a constant water density of 1.025 g/cc and a constant sediment grain density of 2.5 g/cc, the water and sediment masses are converted to volumes. The ratio of the water volume to the total volume is the porosity. The process step took place over a time period of 2002 to 2024 where the analyses were run in the 1 year post core collection for each field expedition. The process date represents the most recent completion of the work.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>SO4_mM (Pore water dissolved sulfate); 0.5 mL porewater was sub-sampled into 2 mL microcentrifuge tubes and dosed with 50 uL 0.1M phosphoric acid. Samples were measured using ion chromatography. IAPSO certified seawater was used for standardization, and precision is ±1.5%. The process step took place over a time period of 2002 to 2024 where the analyses were run in the 1 year post core collection for each field expedition. The process date represents the most recent completion of the work.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>CH4_uM (Pore water dissolved methane); Sediment plugs (volume = 3-6 cm3) were sampled and stored in serum bottles sealed with 1-cm-thick butyl rubber septa. 5mL of saturated brine was added to the serum bottles and shaken to equilibrate the headspace. The samples were refrigerated until analysis. For cruises PCG02-08, Hydrates2004, and AT50-14, methane concentrations of serum bottle headspaces were determined by gas chromatography flame ionization detection. These headspace concentrations were processed into dissolved concentrations of methane in porewater. For samples measured by GC, dissolved concentrations were calculated following Hoehler et al. (2000). For cruise AT50-29B, methane concentrations were determined using cavity ringdown spectroscopy. Check standards for concentration were run approximately every 5 samples and corrected as described by Pohlman and Casso (2021). For samples measured by cavity ring-down spectroscopy, dissolved concentrations were calculated using the following equation: ((pCH4 * 10^-6 * Vg) /  (R * T_extraction) + (Vw * pCH4 * 10^-6 *  Sol_CH4)) / Vw * 10^6 = [CH4] Where pCH4 is the headspace concentration of methane in ppm, Vg is the volume of gas sample in mL, R is the gas constant in L-atm/mol-K, T_extraction is the extraction temperature in Kelvin, Vw is the volume of water sample that was extracted in mL, Sol_CH4 is the solubility of methane in mol/L-atm (Wiesenburg &amp; Guinasso, 1979), and [CH4] is the dissolved concentration of methane in the original natural water sample, in micromoles per liter. The process step took place over a time period of 2002 to 2024 where the analyses were run in the 1 year post core collection for each field expedition. The process date represents the most recent completion of the work.

References:

Hoehler, T., Borowski, W., Alperin, M., Rodriguez, N., and Paull, C., 2000, Model, stable isotope, and 727 radiotracer characterization of anaerobic methane oxidation in gas hydrate-bearing sediments 728 of the Blake Ridge: Scientific Results, 164, https://doi.org/10.2973/odp.proc.sr.164.242.2000.

Pohlman, J.W., and Casso, M., 2021, Comparison of methane concentration and stable carbon isotope data for natural samples analyzed by discrete sample introduction module - cavity ring down spectroscopy (DSIM-CRDS) and traditional methods: U.S. Geological Survey data release, https://doi.org/10.5066/P99B34V1.

Wiesenburg, D. A., and Guinasso Jr, N.L., 1979, Equilibrium solubilities of methane, carbon monoxide, and hydrogen in water and sea water: Journal of Chemical and Engineering Data, 24(4), 356-360, https://doi.org/10.1021/je60083a006.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>DOC_uM (Pore water dissolved organic carbon) and DOC_d13C_permille (Bulk stable carbon isotope value of pore water dissolved organic carbon); Samples (2-4 mL) were stored in combusted borosilicate glass vials with acid-cleaned teflon-lined septa caps. Vials were pretreated with 20 percent hydrochloric acid (12 microliters per milliliter of sample) to decrease the pH of the sample to 2 or less, and samples were stored at 4 degrees Celsius until analysis. Concentrations were determined with a TOC Analyzer using wet chemical oxidation (cruises PGC02-08 and Hydrates2004) (Heuer et al., 2009; Pohlman et al., 2011) and high temperature catalytic oxidation (cruises AT50-14 and AT50-29B) to convert DOC to CO2. For all cruises, nondispersive infrared detection was used to quantify this CO2. Potassium hydrogen phthalate (KHP) calibration standards were run to quantify DOC concentration. Stable carbon isotopes (d13C-DOC) were measured by isotope ratio mass spectrometry. Stable carbon isotope ratios are reported in standard d notation relative to Vienna Pee Dee Belemnite (VPDB). Quality assurance included replicate analysis of natural reference materials, field samples and calibration standards. Uncertainty in DOC concentration was 5 percent or less, and uncertainty in d13C was 0.2 permille or less, based on replicate measurements. The process step took place over a time period of 2002 to 2024 where the analyses were run in the 1 year post core collection for each field expedition. The process date represents the most recent completion of the work.

References:

Heuer, V.B., Pohlman, J.W., Torres, M.E., Elvert, M., and Hinrichs, K.U., 2009, The stable carbon isotope biogeochemistry of acetate and other dissolved carbon species in deep subseafloor sediments at the northern Cascadia Margin: Geochimica et Cosmochimica Acta, 73(11), 3323-3336, https://doi.org/10.1016/j.gca.2009.03.001.

Pohlman, J.W., Bauer, J.E., Waite, W.F., Osburn, C.L., and Chapman, N.R., 2011, Methane hydrate-bearing seeps as a source of aged dissolved organic carbon to the oceans: Nature Geoscience, 4(1), 37-41, https://doi.org/10.1038/ngeo1016.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>DIC_mM (Porewater dissolved inorganic carbon) and DIC_d13C_permille (Bulk stable carbon isotope value of porewater dissolved inorganic carbon); Samples for DIC concentration for cruises PCG02-08 and Hydrates2004 were collected as 2 ml aliquots in 5 ml serum vials, sealed with butyl rubber septa, and quantified by coulometry.  Samples for stable carbon isotopes (d13C-DIC) for cruises PGC02-08 and Hydrates2004 were collected as 1 ml aliquots in 2 ml serum vials, sealed with butyl rubber septa and analyzed as CO2 by isotope ratio mass spectrometry following acidification of the sample (Pohlman et al., 2013). Samples for DIC concentration and stable carbon isotopes for DIC for cruises AT-50-14 and AT50-29B were collected by injecting 1mL porewater into a helium-filled 12 mL exetainer vial pre-filled with 1mL 85% phosphoric acid. The stable carbon isotope ratios and concentrations were determined from CO2 by isotope ratio mass spectrometry. Measurements are standardized with lithium carbonate reference material, and isotope ratios are reported in the standard delta notation relative to VPDB. The process step took place over a time period of 2002 to 2024 where the analyses were run in the 1 year post core collection for each field expedition. The process date represents the most recent completion of the work.

Reference: 

Pohlman, J.W., Riedel, M., Bauer, J.E., Canuel, E.A., Paull, C.K., Lapham, L., Grabowski, K.S., Coffin, R.B., and Spence, G.D., 2013, Anaerobic methane oxidation in low-organic content methane seep sediments: Geochimica et Cosmochimica Acta, 108, 184-201, https://doi.org/10.1016/j.gca.2013.01.022.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>TOC_wt.% (Sediment total organic carbon content) and TOC_d13C_permille (Bulk stable carbon isotope value of pediment total organic carbon content); Sediment samples from all cruises were freeze-dried and ground with a mortar and pestle. Homogenized sediment was weighed into silver capsules for acid fumigation. Samples were acid fumigated to remove carbonate and dried at 45 degrees C for 24 hours, then wrapped into tin capsules. Samples were run on an elemental analyzer interfaced with an isotope ratio mass spectrometer. Samples from PGC02-08 were normalized to an acetanilide standard and samples from AT50-14 were run with a peptone reference material, as well as standards USGS 40 226 and USGS 41. Stable carbon isotope values are reported in d notation relative to VPDB. The process step took place over a time period of 2004 to 2024 where the analyses were run in the 1 year post core collection for each field expedition. The process date represents the most recent completion of the work.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
   <procstep>
    <procdesc>H2S_mM (Pore water dissolved sulfide); Porewater sulfide (ΣH2S) was determined by sparging the sample aliquot after acidification with 25 % phosphoric acid, and trapping evolved H2S in sulfide antioxidant buffer (SAOB) solution for measurement with a sulfide specific electrode. Standards were prepared from a sodium bisulfide stock solution that was titrated with lead nitrate to determine its concentration, daily. Also on a daily basis, the stock solution was serially diluted to produce a five-level calibration. The process step took place over a time period of 2023 to 2024 with the analyses for a particular cruise run onboard the ship during the cruise. The process date represents the most recent completion of the work.</procdesc>
    <procdate>2024</procdate>
    <proccont>
     <cntinfo>
      <cntperp>
       <cntper>Ellen Lalk</cntper>
       <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
      </cntperp>
      <cntpos>Research Chemist</cntpos>
      <cntaddr>
       <addrtype>mailing and physical address</addrtype>
       <address>384 Woods Hole Rd.</address>
       <city>Woods Hole</city>
       <state>MA</state>
       <postal>02543-1598</postal>
      </cntaddr>
      <cntvoice>508-548-8700</cntvoice>
      <cntemail>elalk@usgs.gov</cntemail>
     </cntinfo>
    </proccont>
   </procstep>
  </lineage>
 </dataqual>
 <spdoinfo>
  <direct>Point</direct>
  <ptvctinf>
   <sdtsterm>
    <sdtstype>Point</sdtstype>
    <ptvctcnt>126</ptvctcnt>
   </sdtsterm>
  </ptvctinf>
 </spdoinfo>
 <spref>
  <horizsys>
   <geograph>
    <latres>0.0001</latres>
    <longres>0.0001</longres>
    <geogunit>decimal degrees</geogunit>
   </geograph>
   <geodetic>
    <horizdn>D_WGS_1984</horizdn>
    <ellips>WGS_1984</ellips>
    <semiaxis>6378137.000000</semiaxis>
    <denflat>298.257224</denflat>
   </geodetic>
  </horizsys>
  <vertdef>
   <depthsys>
    <depthdn>local surface - depth below sea floor</depthdn>
    <depthres>0.5</depthres>
    <depthdu>centimeters</depthdu>
    <depthem>attribute values</depthem>
   </depthsys>
  </vertdef>
 </spref>
 <eainfo>
  <detailed>
   <enttyp>
    <enttypl>"Discrete_GeochemicalData_CascadiaMarginSeeps"</enttypl>
    <enttypd>Microsoft Excel workbook that contains tabulated location and property data for specimens collected and published as part of this Cascadia Margin methane seep study.</enttypd>
    <enttypds>producer defined</enttypds>
   </enttyp>
   <attr>
    <attrlabl>Region</attrlabl>
    <attrdef>General sampling location within the Cascadia Margin (Astoria Canyon, Barkley Canyon, Hydrate Ridge, Bullseye Vent).</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <udom>user defined text field</udom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>Cruise</attrlabl>
    <attrdef>Cruise ID for sample collection with the following values: PGC02-08 (CCGS John P. Tully, 2002), Hydrates2004 (CCGS John P. Tully, 2004, Hydrates 2004 Sea Trial), AT50-14 (R/V Atlantis, 2023), and AT50-29B (R/V Atlantis, 2024)</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <udom>user defined text field</udom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>CoreID</attrlabl>
    <attrdef>Core IDs are specific letter and number combinations given to cores when collected, and are specific to each individual cruise.</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <udom>user defined text field</udom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>SampleID</attrlabl>
    <attrdef>Sample IDs are numbers given to each sediment sub-sample when collected, and are specific to each individual cruise.</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <udom>user defined text field</udom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>CoreType</attrlabl>
    <attrdef>Core type specifies the environment of the core collection (seep = collected in a methane seep, reference = collected outside a methane seep).</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <udom>user defined</udom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>Latitude</attrlabl>
    <attrdef>Sample latitude, south is negative (decimal degrees).</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>44.568359</rdommin>
      <rdommax>48.671875</rdommax>
      <attrunit>decimal degrees</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>Longitude</attrlabl>
    <attrdef>Sample longitude, west is negative (decimal degrees).</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>-126.849944</rdommin>
      <rdommax>-124.6027</rdommax>
      <attrunit>decimal degrees</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>WaterDepth_m</attrlabl>
    <attrdef>Depth of the sea floor relative to the sea surface at the sample location (meters, m). Based on shipboard tidally corrected values.</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>787</rdommin>
      <rdommax>1282</rdommax>
      <attrunit>meters</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>MidDepth_cmbsf</attrlabl>
    <attrdef>Mid-depth of core sub-section relative to the sediment water interface (centimeters below sea floor, cmbsf).</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>0.75</rdommin>
      <rdommax>818</rdommax>
      <attrunit>centimeters below sea floor (cmbsf)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>Porosity</attrlabl>
    <attrdef>Sediment water volume relative to total specimen volume (unitless). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>0.64</rdommin>
      <rdommax>0.9</rdommax>
      <attrunit>unitless</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>SO4_mM</attrlabl>
    <attrdef>Pore water dissolved sulfate (millimolar, mM). NaN = “Not a Number (no data value).” BDL = “Below Detection Level.”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>0</rdommin>
      <rdommax>31.07346</rdommax>
      <attrunit>millimolar (mM)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>CH4_uM</attrlabl>
    <attrdef>Pore water dissolved methane (micromolar, uM). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>0.001346</rdommin>
      <rdommax>26907.1</rdommax>
      <attrunit>micromolar (uM)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>DOC_uM</attrlabl>
    <attrdef>Pore water dissolved organic carbon (micromolar, uM). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>283.3</rdommin>
      <rdommax>3600</rdommax>
      <attrunit>micromolar (uM)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>DOC_d13C_permil</attrlabl>
    <attrdef>Bulk stable carbon isotope value of pore water dissolved organic carbon (per mille) relative to Vienna Pee Dee Belemnite (VPDB). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>-60.44</rdommin>
      <rdommax>-7.46</rdommax>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>DIC_mM</attrlabl>
    <attrdef>Pore water dissolved inorganic carbon (millimolar, mM). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>1.3</rdommin>
      <rdommax>27.49</rdommax>
      <attrunit>millimolar (mM)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>DIC_d13C_permil</attrlabl>
    <attrdef>Bulk stable carbon isotope value of pore water dissolved inorganic carbon (per mille) relative to Vienna Pee Dee Belemnite (VPDB). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>-52.62</rdommin>
      <rdommax>11.89</rdommax>
      <attrunit>per mille</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>TOC_wt.%</attrlabl>
    <attrdef>Sediment total organic carbon content (weight percent, wt.%). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>2.54</rdommin>
      <rdommax>3.18</rdommax>
      <attrunit>weight percent (wt.%)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>TOC_d13C_permil</attrlabl>
    <attrdef>Bulk stable carbon isotope value of pediment total organic carbon content (per mille) relative to Vienna Pee Dee Belemnite (VPDB). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>-26.05</rdommin>
      <rdommax>-22.91</rdommax>
      <attrunit>per mille</attrunit>
     </rdom>
    </attrdomv>
   </attr>
   <attr>
    <attrlabl>H2S_mM</attrlabl>
    <attrdef>Pore water dissolved sulfide (millimolar, mM). NaN = “Not a Number (no data value).”</attrdef>
    <attrdefs>U.S. Geological Survey</attrdefs>
    <attrdomv>
     <rdom>
      <rdommin>0.00096</rdommin>
      <rdommax>21.1</rdommax>
      <attrunit>millimolar (mM)</attrunit>
     </rdom>
    </attrdomv>
   </attr>
  </detailed>
  <overview>
   <eaover>The workbook Discrete_GeochemicalData_CascadiaMarginSeeps.xlsx contains one tab. The first row in the XLSX file is a header row. The remaining rows contain all data included in this data release, with non-measurements and undetected quantities given by “NaN,” and “BDL” as indicated in the attribute definitions in this xml file. Additionally, a CSV file “parameters_CascadiaMarginSeeps.csv” provides definitions for the attributes in the Discrete_GeochemicalData_CascadiaMarginSeeps.xlsx file. Definitions in the CSV file follow an initial header row. These files are part of a data release posted to the BCO-DMO data repository (Biological and Chemical Oceanography Data Management Office, BCO-DMO)</eaover>
   <eadetcit>Lalk, E., Pohlman, J. W., Lapham, L. L., Casso, M., Glenna, M., Hildebrand, A., Lloyd, K., Ma, K., Seewald, J., Stock, L., Strauss, M. A., Sylva, S., Traver, L., Wilson, R., Williams, L., 2025, Porewater dissolved organic carbon and associated geochemical data for methane seeps in the Cascadia Margin—Astoria Canyon, Barkley Canyon, Hydrate Ridge, and Bullseye Vent: Biological and Chemical Oceanography Data Management Office (BCO-DMO). https://doi.org/10.26008/1912/bco-dmo.959765.1 .</eadetcit>
  </overview>
 </eainfo>
 <distinfo>
  <distrib>
   <cntinfo>
    <cntorgp>
     <cntorg>Biological and Chemical Oceanography Data Management Office (BCO-DMO)</cntorg>
    </cntorgp>
    <cntaddr>
     <addrtype>mailing and physical address</addrtype>
     <address>Biological and Chemical Oceanography Data Management Office BCO-DMO, Woods Hole Oceanographic Institution, Shiverick House, MS #36</address>
     <city>Woods Hole</city>
     <state>Massachusetts</state>
     <postal>02543</postal>
     <country>USA</country>
    </cntaddr>
    <cntvoice>+1 508 289 2772</cntvoice>
    <cntemail>info@bco-dmo.org</cntemail>
   </cntinfo>
  </distrib>
  <resdesc>The Biological and Chemical Oceanography Data Management Office (BCO-DMO) is a trustworthy, publicly-accessible, domain science data repository created to curate, publish, and archive digital data and information from biological, chemical, and biogeochemical research conducted in coastal, marine, Great Lakes, and laboratory environments. The office provides services that span the full data life cycle, from data management planning support and DOI creation, to archive with appropriate long-term facilities. Repository staff work closely with investigators to help them prepare, publish, and share their data and related information for reuse. Views and opinions expressed by BCO-DMO belong solely to the project PIs and its team members, and do not necessarily reflect those of project funders.</resdesc>
  <distliab>Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.</distliab>
  <stdorder>
   <digform>
    <digtinfo>
     <formname>XLSX</formname>
     <formvern>Created in Excel Office 365 version 2408</formvern>
     <transize>1</transize>
    </digtinfo>
    <digtopt>
     <onlinopt>
      <computer>
       <networka>
        <networkr>https://doi.org/10.26008/1912/bco-dmo.959765.1</networkr>
       </networka>
      </computer>
      <accinstr>The link is the doi to the data release landing page where the data can be downloaded.</accinstr>
     </onlinopt>
    </digtopt>
   </digform>
   <digform>
    <digtinfo>
     <formname>CSV</formname>
     <formvern>Created in Excel Office 365 version 2408</formvern>
     <transize>1</transize>
    </digtinfo>
    <digtopt>
     <onlinopt>
      <computer>
       <networka>
        <networkr>https://doi.org/10.26008/1912/bco-dmo.959765.1</networkr>
       </networka>
      </computer>
      <accinstr>The link is the doi to the data release landing page where the data can be downloaded.</accinstr>
     </onlinopt>
    </digtopt>
   </digform>
   <fees>None.</fees>
  </stdorder>
 </distinfo>
 <metainfo>
  <metd>20250522</metd>
  <metc>
   <cntinfo>
    <cntperp>
     <cntper>Brian J. Buczkowski</cntper>
     <cntorg>U.S. Geological Survey, Northeast Region, Woods Hole Coastal and Marine Science Center</cntorg>
    </cntperp>
    <cntpos>Supervisory Physical Scientist</cntpos>
    <cntaddr>
     <addrtype>mailing and physical</addrtype>
     <address>U.S. Geological Survey</address>
     <address>384 Woods Hole Rd.</address>
     <city>Woods Hole</city>
     <state>MA</state>
     <postal>02543-1598</postal>
    </cntaddr>
    <cntvoice>508-548-8700 x2361</cntvoice>
    <cntemail>whsc_data_contact@usgs.gov</cntemail>
    <cntinst>The metadata contact email address is a generic address in the event the person is no longer with USGS.</cntinst>
   </cntinfo>
  </metc>
  <metstdn>Content Standard for Digital Geospatial Metadata</metstdn>
  <metstdv>FGDC-STD-001-1998</metstdv>
  <metac>None</metac>
  <metuc>None</metuc>
 </metainfo>
</metadata>
