Koch, Joshua C. (ORCID: 0000-0001-7180-6982) Couvillion, Charles S. (ORCID: 0009-0006-6187-8708) Best, Heather R. (ORCID: 0000-0003-0764-3060) Carey, Michael P. (ORCID: 0000-0002-3327-8995) 20230719 tabular digital data Macroinvertebrates from Streams and Springs in the 1002 Region of the Arctic National Wildlife Refuge, Alaska, 2021 ver 1.1, January 2026 Anchorage, Alaska U.S. Geological Survey, Alaska Science Center Suggested Citation: Koch, J.C., Couvillion, C., Best, H., Carey, M.P., 2023, Macroinvertebrates from streams and springs in the 1002 region of the Arctic National Wildlife Refuge, Alaska, 2021 (ver 1.1, January 2026): U.S. Geological Survey data release, https://doi.org/10.5066/F7X34VHM https://doi.org/10.5066/F7X34VHM This dataset includes tables related to macroinvertebrate collections in streams and springs of the 1002 region of the Arctic National Wildlife Refuge in northeastern Alaska. Macroinvertebrates were collected using kicknets and driftnets in April and August with the goal of comparing populations to those sampled from the same water bodies by the USGS in the 1970s. These data were collected to compare macrovinvertebrate populations in time. 20210421 20210817 observed Complete None planned Coastal Plain and foothills of the Arctic National Wildlife Refuge, Alaska -146.45 -142.95 69.87 69.60 USGS Metadata Identifier USGS:ASC546 ISO 19115 Topic Category Biota Environment InlandWaters NASA GCMD Earth Science Keyword Thesaurus Animals/Invertebrates Arthropods USGS CSA Biocomplexity Thesaurus Animals/Invertebrates Arthropods Age composition USGS Thesaurus Macroinvertebrates Aquatic ecosystems Freshwater ecosystems Community ecology None ANWR 1002 Area USGS Geographic Names Information System (GNIS) Alaska North Slope USGS Protected Areas Database of the United States (PAD-US) Arctic National Wildlife Refuge None Fish Aquatic Invertebrates None Bivalvia Clitellata Collembola Euchelicerata Arachnida Gastropoda Insecta Malacostraca ITIS Integrated Taxonomic Information System Unknown online database online ITIS-North America Taxonomic details retrieved May 9, 2023, from the Integrated Taxonomic Information System online database https://www.itis.gov https://doi.org/10.5066/F7KH0KBK Species were identified by the National Aquatic Monitoring Center Provided here is the classification of animal taxa to the rank of Class. Further details of taxonomy are provided in the table "taxonomy_ANWR_Hydro_Koch.csv" included with this data package. Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Lophozoa Phylum Mollusca Class Bivalvia TSN: 79118 Class Gastropoda TSN: 69459 Phylum Annelida Class Clitellata TSN: 568832 Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda Class Collembola TSN: 914185 Class Insecta TSN: 99208 Subphylum Chelicerata Class Euchelicerata TSN: 1117839 Subclass Arachnida TSN: 1117840 Subphylum Crustacea Class Malacostraca TSN: 89787 No access constraints. No use constraints. These data are marked with a Creative Commons Zero v1.0 (CC0-1.0) Universal public domain dedication. It is requested that this USGS data release be cited for any subsequent publications that reference or utilize these data. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov Koch, J.C. Best, H.R. Baughman, C. Couvillion, C.S. Carey, M.P. Conaway, J. 2024 publication U.S. Geological Survey Scientific Investigation Report 2024–5008 online U.S. Geological Survey Koch, J.C., Best, H., Baughman, C., Couvillion, C., Carey, M.P., Conaway, J., 2024, A comparison of contemporary and historical hydrology and water quality in the foothills and coastal plain of the Arctic National Wildlife Refuge, Arctic Slope, northern Alaska: U.S. Geological Survey Scientific Investigations Report 2024–5008, 24 p., https://doi.org/10.3133/sir20245008 https://doi.org/10.3133/sir20245008 The macroinvertebrate samples were analyzed by the National Aquatic Monitoring Center, which report QA/QC methods at: https://namc-usu.org/SampleProcessing/QualityStandards. Attribute values fall within expected ranges. The dataset is complete, no data were omitted. FIELD: Macroinvertebrates were collected in streams, springs, and rivers following standard methods [Cuffney et al., 1993; Merritt and Cummins, 1996]. Invertebrates were collected via an hour-long deployment of a Surber net as a drift sampler. The net was placed in area of moderate flow with the bottom of the net frame against the streambed and the top of the net frame above the water surface to collect surface film. After collection of the drift sample, the invertebrates were sampled at eight locations within the channel by agitating the bottom materials within the frame attached to the Surber net for one minute at each location. There were no field splits. Unknown LAB: Samples were shipped to the National Aquatic Monitoring Center, where they were identified to species (Merrit et al 2008), following methods published at https://namc-usu.org/SampleProcessing/QualityStandards Unknown LITERATURE CITED: Cuffney, T.F., Gurtz, M.E., Meador, M.R., 1993. Guidelines for the processing and quality assurance of benthic invertebrate samples collected as part of the National Water-Quality Assessment Program: U.S. Geological Survey Open-File Report 93-407, https://doi.org/10.3133/ofr93407 Merritt, R.W., Cummins, K.W., Berg, M.B., 2008. An Introduction to the Aquatic Insects of North America 4th edition, Kendall Hunt Inc., Dubuque, IA Unknown Point 0.01 0.01 Decimal degrees World Geodetic System of 1984 (WGS84) World Geodetic System of 1984 (WGS84) 6378137 298.257223563 macroinvertebrate_streamSampling_ANWR_koch.csv Table with data related to sampling locations and dates, watershed names and types, and the collected sample type and volume. Presented in a Comma Separated Value (CSV) formatted table. Author defined Sample ID Unique identifier: numerical code that links locations in this csv to analytical data in other csv and searchable at NAMC (https://namc-usu.org/data) Author defined 185705 185737 Watershed The name of the river or spring in which the sample was collected. Author defined Aichilik River Sampling sites in the Aichilik River (approximately 69.3523 N, 142.5803 W) Author defined Canning River Sampling site in the Canning River (approximately) Author defined Hulahula River Two sampling site in the Hulahula River (approximately Author defined Jago River Sampling site in the Jago River (approximately Author defined Kataturuk River Two sampling sites in the Kataturuk River (approximately Author defined Marsh Creek Sampling site in Marsh Creek (approximately Author defined Okerokovik River Sampling site in the Okerokovik River (approximately Author defined Red Hill Spring Sampling site near the Canning River(approximately Author defined Sadlerochit Spring Sampling site in the Sadlerochit Spring (approximately Author defined Latitude Latitude of sampling location. Author defined 69.60 69.87 Decimal Degrees Longitude Longitude of sampling location. Author defined -146.45 -142.95 Decimal Degrees Collection_date The date on which the sample was collected Author defined 2021-04-21 2021-08-17 yyyy-mm-dd Sample_Type Indicates whether the sample was a drift sample collected with a drift net or a benthic sample collected with a kick net. Author defined Drift A sample collected with a drift net Author defined Benthic A sample collected with a kick net Author defined Volume The volume of water sampled for a drift sample determined based on the net area, water velocity, and deployment duration. Author defined 64 546 cubic meters NA NA values appear for benthic samples where an area was recorded rather than a volume. Producer defined Area The surface area of the sediment that was disturbed to obtain the kick net sample. Author defined 0.24 0.24 square meters NA NA values appear for drift samples where an volume was recorded rather than an area. Producer defined Lab_Split The fraction of the total volume that the laboratory used for the analysis Author defined 0.125 1 unitless Split_Count The number of individual invertebrates counted within the split fraction Author defined 4 1680 number of invertebrates macroinvertebrate_streamCounts_ANWR_koch.csv Table containing the number of macroinvertebrates counted in each sample. Samples were identified to the lowest possible resolution. Depending on the invertebrate, samples were identified to the subclass, order, family, genus, or species. Author defined Sample ID Unique identifier: numerical code that links count data in this csv to location data in the other csv and searchable at NAMC (https://namc-usu.org/data) Author defined 185705 185737 Scientific_Name Scientific name of the identified taxa. Author defined Scientific name of the taxa. Order Taxonomic hierarchical level; order. ITIS Taxonomic hierarchical level; order Family Taxonomic hierarchical level; family. ITIS Taxonomic hierarchical level; family Genus Taxonomic hierarchical level; genus. ITIS Taxonomic hierarchical level; genus Species Taxonomic hierarchical level; species ITIS Taxonomic hierarchical level; species lifeStage The life stage of the invertebrate. ITIS Larvae The observed invertebrates was in the larval stage of development Author defined Pupae The observed invertebrates was in the pupal stage of development Author defined Adult The observed invertebrates was in the adult stage of development Author defined splitCount The number of individuals counted in the sample. Author defined 0 1255 bigRareCount The number of big and rare organsisms selected non-randomly from the full sample. Author defined 0 0 macroinvertebrate_taxonomy_ANWR_koch.csv Table containing the taxonomic classification of invertebrates identified in the other tables of this data package. Presented in a Comma Separated Value (CSV) formatted table. Missing values exist when an organism was not identified to the taxonomic level. Author defined Taxon_Name Scientific name of the taxa identified in the other tables of this data package. Author defined Scientific name of the taxa. Kingdom Taxonomic hierarchical level; kingdom ITIS Taxonomic hierarchical level; kingdom Phylum Taxonomic hierarchical level; phylum ITIS Taxonomic hierarchical level; phylum Class Taxonomic hierarchical level; class ITIS Taxonomic hierarchical level; class Order Taxonomic hierarchical level; order. ITIS Taxonomic hierarchical level; order Family Taxonomic hierarchical level; family. ITIS Taxonomic hierarchical level; family Genus Taxonomic hierarchical level; genus. ITIS Taxonomic hierarchical level; genus Species Taxonomic hierarchical level; species ITIS Taxonomic hierarchical level; species TSN Taxonomic Serial Number; unique identifier of taxa maintained by Integrated Taxonomic Information System (ITIS). ITIS Taxonomic Serial Number U.S. Geological Survey USGS ScienceBase Team Mailing and Physical

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Denver Colorado 80225 USA 1-888-275-8747 sciencebase@usgs.gov The U.S. Geological Survey, Alaska Science Center is the authoritative source of these data, distributed by ScienceBase (a USGS Trusted Digital Repository). Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data for other purposes or on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. CSV Tabular data in CSV format; FGDC metadata in XML and HTML formats. https://doi.org/10.5066/F7X34VHM None 20260108 U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata (CSDGM) FGDC-STD-001.1-1999