Talbot, Sandra L. (ORCID: 0000-0002-3312-7214) Pierson, Barabara J. (ORCID: 0000-0001-8233-874X) Sonsthagen, Sarah A. (ORCID: 0000-0001-6215-5874) Haughey, Christy L. (ORCID: 0000-0002-4846-6008) 20200921 tabular digital data Anchorage, Alaska U.S. Geological Survey, Alaska Science Center Suggested Citation: Talbot, S.L., Pierson, B.J., Sonsthagen, S.A., Haughey, C.L., 2020, Genetic data from three accipiter species, North America, 1996-2014: U.S. Geological Survey data release, https://doi.org/10.5066/P9U28F8X https://doi.org/10.5066/P9U28F8X This data set provides sample collection information and genetic data used to identify relationships between groups of North American accipiters, including one hybrid. Genetic data includes gender identification, nuclear and mtDNA sequence markers, and microsatellite genotypes derived from 20 autosomal loci (Age1302, Age1303, Age1304, Age1305, Age1306, Age1307, Age1308, Age1309, Age1310, Age1311, Age1312, Age1313, Age1314, Age1316, Age1317, Age1318, Age1319, Age1320, Age1321, and Age1322). These data were collected to measure the usefulness of new microsatellite markers in three North American accipiter species and assess the existence of a suspected naturally occurring hybrid. 201301 201911 observed Complete None planned From New Jersey in the east to Alaska in the west and from Utah in the south to Yukon Territory, Canada in the North -149.18 -74.90 69.63 38.03 USGS Metadata Identifier USGS:ASC314 ISO 19115 Topic Category Biota Environment NASA GCMD Earth Science Thesaurus Animals/Vertebrates Birds Eagles/Falcons/Hawks and Allies USGS CSA Biocomplexity Thesaurus Birds of prey Genetic diversity Population genetics Genetic variance Hybrids USGS Thesaurus Wildlife Ecological processes Genetics DNA sequencing Polymerase chain reaction Systematics and taxonomy MeSH-National Library of Medicine DNA, Mitochondrial Microsatellite Repeats Genotype None Northern Goshawk Accipiter gentilis Cooper's Hawk Accipiter cooperii Sharp-shinned Hawk Accipiter striatus USGS Geographic Names Information System (GNIS) Alaska Utah Minnesota Wisconsin New Jersey North Dakota NGA GEOnet Names Server Canada British Columbia Yukon Territory None Animals Vertebrates Birds ITIS Integrated Taxonomic Information System Unknown online database online ITIS-North America Taxonomic details retrieved August 13, 2022, from the Integrated Taxonomic Information System online database https://www.itis.gov https://doi.org/10.5066/F7KH0KBK Samples were provided by scientists competent to identify genus Accipiter to the species level. DNA data generated in this study would have identified any incorrectly labeled samples. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Aves Order Accipitriformes Family Accipitridae Genus Accipiter Species Accipiter gentilis Northern Goshawk TSN 175300 Species Accipiter striatus Sharp-shinned Hawk TSN 175304 Species Accipiter cooperii Cooper's Hawk TSN 175309 No access constraints. No use constraints. We request that the suggested citation of this USGS data release be included in any publications that reference or utilize these data. U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov U.S. Geological Survey, Alaska Science Center, Anchorage, Alaska, USA; Cape May Raptor Banding Project, 105 Yucca Court, Pine Knoll Shores, North Carolina, USA; Department of Biology, University of Wisconsin-Stevens Point, Stevens Point, Wisconsin, USA; Functional Biosciences, Madison, Wisconsin, USA Haughey, C.L. Sage, G.K. DeGange, G.R. Sonsthagen, S.A. Talbot, S.L. 2016 journal article Avian Biology Research 9(3):195-199 online SAGE Publishing Haughey, C.L., Sage, G.K., DeGange, G.R., Sonsthagen, S.A., Talbot, S.L., 2016. Development of novel microsatellite markers for the Northern Goshawk (Accipiter gentilis) and their utility in cross-species amplification. Avian Biology Research 9(3):195-199, https://doi.org/10.3184/175815516X14667737479433 https://doi.org/10.3184/175815516X14667737479433 Haughey, C.L. Nelson, A. Napier, P. Rosenfield, R.N. Sonsthagen, S.A. Talbot, S.L. 2019 journal article Wilson Journal of Ornithology 131(4):838–844 online Wilson Ornithological Society Haughey, C.L., Nelson, A., Napier, P., Rosenfield, R.N., Sonsthagen, S.A., Talbot, S.L., 2019. Genetic confirmation of a natural hybrid between a Northern Goshawk (Accipiter gentilis) and a Cooper's Hawk (A. cooperii). Wilson Journal of Ornithology 131(4):838–844, https://doi.org/10.1676/1559-4491-131.4.838 https://doi.org/10.1676/1559-4491-131.4.838 Individual sample ID's, collection dates, sampling locations, numbers, and reported sex of individual samples provided by collectors were cross-checked and verified to match the accompanying worksheet. Sampling locations were verified with collectors and formally labeled and cross-referenced to the Geographic Names Information Service (GNIS) when possible. Genbank accession numbers were cross-referenced to the appropriate individual samples as recorded. For quality control purposes, DNA from two to five individuals representing each designated population were extracted, amplified, and sequenced in duplicate. We observed sterile techniques in the handling of all DNA, and all procedures were performed with positive and negative controls to provide evidence of replication without contamination. Per laboratory standard operating procedures, if discrepancies occurred, analyses were repeated until the laboratory manager was satisfied results were accurate. For microsatellite loci, reported allele sizes were reviewed to confirm they conform to expected distributions (e.g. dinucleotide repeats with 1 base pair differences were reviewed on gel images by laboratory personnel, gaps in allele sizes were reviewed, and single occurrences of an allele size were scrutinized). Only representative unique sequences were submitted to Genbank for partial control regions of mtDNA and partial intron 7 of beta-fibrinogen, i.e. multiple individuals may have the same accession number if the sequences are identical to the original sequence submitted to Genbank. Data omissions for particular entities are explained in the Entity and Attribute definitions. Blank cells indicate that data were not collected or not provided with the sample for a particular attribute. Collection years are provided when available, but were not available for all samples provided to the laboratory. Field sex was not provided with all samples, nor were all field sexes confirmed with a laboratory analysis for all individuals. National Center for Biotechnology Information (NCBI) Unknown database https://www.ncbi.nlm.nih.gov/genbank digital database file 2020 observed NCBI GenBank Sequence information are publicly accessible in the NCBI Genbank database (accession numbers provided in this data package: "accipter_geneticData_Haughey_2020.csv") LAB: DNAs were extracted by a modified salt extraction technique (Medrano et al 1990; Sonsthagen et al 2004) or using a Qiagen Dneasy Blood and Tissue extraction kit (QIAGEN, Düsseldorf, Germany) following manufacturer's protocol. Polymerase chain reaction (PCR) amplification and scoring of microsatellite loci followed Sonsthagen et al 2004 except the thermocycler parameters consisted of a 2 min denaturation at 94 °C, followed 40 cycles at 94°C for 15 seconds, 50°C for 15 seconds and 72 °C for 30 seconds with a final extension at 72 °C for 30 min. Microsatellite alleles were resolved on a 25 cm 6% polyacrylamide gel using a LI-COR (IR 4200) automated DNA sequencer. For quality control purposes, we extracted, amplified, and sequenced, 10% of the samples in duplicate. Polymerase chain reaction (PCR) amplification and sequencing of mtDNA control region followed Sonsthagen et al 2004 and beta-fibrinogen intron 7 loci followed Sonsthagen et al 2007 with PCR conditions following Sonsthagen et al 2007. Sequences were generated and resolved either with simultaneous bidirectional sequencing methods at the Alaska Science Center as described in Sonsthagen et al 2004 or at Functional Biosciences (Madison, Wisconsin, USA) using methods described in Sonsthagen et al 2007. For quality control purposes, we extracted, amplified, and sequenced, 10% of the samples in duplicate. Unknown Initial microsatellite and CHD allele sizes were determined by scoring based on PCR product size relative to an M13 DNA sequence ladder using Gene Profiler 4.05 (Scanalytics, Inc., Fairfax, VA). Subsequent gels included these known size individuals from which relative sizes of unknown individuals were assigned using Gene Profiler 4.05. For this reason, these microsatellite data cannot be combined with data generated outside the USGS Alaska Science Center without molecular size calibration between laboratories. DNA sequences were reconciled using Sequencher 4.1.2 (Gene Codes Corporation, Ann Arbor, MI) or using LI-COR eSeq imaging software with visual proofing and aligned using AlignIR 2.0 (LI-COR, Inc., Lincoln, NE). Unknown Representative sequences of 419 bp (JQ894325-JQ894327) or 593 bp (KU659807) of mtDNA and of 298 bp of beta-fibrinogen intron 7 were submitted to the NCBI Genbank publicly accessible genetic sequence data database. Unknown NCBI GenBank LITERATURE CITED: Haughey, C.L., Sage, G.K., DeGange, G.R., Sonsthagen, S.A., Talbot, S.L., 2016. Development of novel microsatellite markers for the Northern Goshawk (Accipiter gentilis) and their utility in cross-species amplification. Avian Biology Research 9(3):195-199, https://doi.org/10.3184/175815516X14667737479433 Haughey, C.L., Nelson, A., Napier, P., Rosenfield, R.N., Sonsthagen, S.A., Talbot, S.L. 2019. Genetic confirmation of a natural hybrid between a Northern Goshawk (Accipiter gentilis) and a Cooper's Hawk (A. cooperii). Wilson Journal of Ornithology 131(4):838–844, https://doi.org/10.1676/1559-4491-131.4.838 Medrano, J.F., Aasen, E., Sharrow, L., 1990. DNA extraction from nucleated red blood cells. Biotechniques 8(1):43 Sonsthagen, S.A., Talbot, S.L., White, C.M., 2004. Gene flow and genetic characterization of Northern Goshawks breeding in Utah. Condor 106(4):826–836, https://www.jstor.org/stable/3247787 Sonsthagen, S.A., Talbot, S.L., McCracken, K.G., 2007. Genetic characterization of Common Eiders breeding in the Yukon-Kuskokwim Delta, Alaska. Condor 109(4):878-893, https://doi.org/10.1093/condor/109.4.878 Unknown States and Provinces of sample collection are listed to describe generalized field sampling locations. accipiter_microsat_NAmer_haughey.csv Table with sample identifying information, collection data, allele scores (in base pairs) for 20 autosomal microsatellite loci (Age1302, Age1303, Age1304, Age1305, Age1306, Age1307, Age1308, Age1309, Age1310, Age1311, Age1312, Age1313, Age1314, Age1316, Age1317, Age1318, Age1319, Age1320, Age1321, and Age1322), and partial information for field and molecular sexes, mtDNA control region and beta-fibrinogen intron 7 sequence accessions. Presented in a Comma Separated Value (CSV) formatted table. Author defined labID USGS Alaska Science Center, Molecular Ecology Laboratory (MEL) alphanumeric label that uniquely identifies each sample. Author defined Unique identifier; an alphanumeric code for each individual sample in this dataset. species This indicates the species of a sample based upon field identification and verified by genetic analyses. Author defined Accipiter gentilis Scientific name for Northern Goshawk Author defined Accipiter cooperii Scientific name for Cooper's Hawk Author defined Accipiter striatus Scientific name for Sharp-shinned Hawk Author defined NOGOxCOHA Putative hybrid of a Northern Goshawk and Cooper's Hawk Author defined collection_start The starting date of the field sample collection. Zero-zero's (00) in month and day digits indicate only the sampling year is known. Blank cells indicate information is not available. Author defined 19960501 20140907 Date (YYYYMMDD) collection_end The ending date of the field sample collection. Blank cells indicate information is not available. Author defined 19960501 20140907 Date (YYYYMMDD) location The state, territory or province of the sampling location Author defined Alaska Sample collected in the state of Alaska, USA. Geographic Names Information System (GNIS) British Columbia Sample collected in province of British Columbia, Canada NGA GEOnet Names Server (GNS) Minnesota Sample collected in the state of Minnesota, USA Geographic Names Information System (GNIS) New Jersey Sample collected in the state of New Jersey, USA Geographic Names Information System (GNIS) North Dakota Sample collected in the state of North Dakota, USA Geographic Names Information System (GNIS) Utah Sample collected in the state of Utah, USA Geographic Names Information System (GNIS) Wisconsin Sample collected in the state of Wisconsin, USA Geographic Names Information System (GNIS) Yukon Territory Sample collected in the Yukon Territory, Canada NGA GEOnet Names Server (GNS) country The country of the sampling location Author defined USA Sample collected in United States of America Geographic Names Information System (GNIS) Canada Sample collected in Canada NGA GEOnet Names Server (GNS) fieldSex The sex reported to the laboratory by the source providing the DNA or DNA source tissue to the laboratory. Author defined M Sample was identified as a male in the field. This information provided to, but not generated by the laboratory. Author defined F Sample was identified as a female in the field. This information provided to, but not generated by the laboratory. Author defined unk The sex of this sample was not provided to the lab. Author defined associated_manuscripts Indicates whether the sample data have been analyzed and presented in any published manuscripts. Blank cells indicate that the sample was not presented in a published manuscript. Author defined A Sample was used in the analysis for Haughey 2020. Author defined Bfib GenBank Accession Number - unique identifier for Intron 7 of the nuclear beta-fibrinogen (FGB) gene sequence, assigned by the National Center for Biotechnology Information (NCBI). Blank cells indicate that sequence data from this locus were not collected for this sample. NCBI GenBank Unique identifier: GenBank accession number assigned by NCBI. Only representative unique sequences were submitted to Genbank for partial control regions of mtDNA and partial intron 7 of beta-fibrinogen, i.e. multiple individuals may have the same accession number if the sequences are identical to the original sequence submitted to Genbank. This number may be used to retrieve detailed sequence information from https://www.ncbi.nlm.nih.gov/ mtDNA GenBank Accession Number - unique identifier for partial mitochondrial DNA (mtDNA) control region nucleotide sequence, assigned by the National Center for Biotechnology Information (NCBI). Blank cells indicate that sequence data from this locus were not collected for this sample. NCBI GenBank Unique identifier: GenBank accession number assigned by NCBI. Only representative unique sequences were submitted to Genbank for partial control regions of mtDNA and partial intron 7 of beta-fibrinogen, i.e. multiple individuals may have the same accession number if the sequences are identical to the original sequence submitted to Genbank. This number may be used to retrieve detailed sequence information from https://www.ncbi.nlm.nih.gov/ CHD_Gametolog1 Gametolog one from the chromo helicase DNA-binding gene (CHD) locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not collected at this locus for that sample. Author defined 385 390 base pairs CHD_Gametolog2 Gametolog two from the chromo helicase DNA-binding gene (CHD) locus: genotype numbers were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. A blank cell indicates data were not collected at this locus for that sample. Author defined 388 390 base pairs Age1302_allele1 through Age1322_allele2 These 40 columns represent twenty autosomal microsatellite loci. Each microsatellite locus genotype is represented by two adjacent columns (i.e., allele1 and allele2) each containing one allele score. Blank cells indicate data were not collected for this sample. NP indicates that no product was amplified from the sample. Locus names and order of presentation: Age1302, Age1303, Age1304, Age1305, Age1306, Age1307, Age1308, Age1309, Age1310, Age1311, Age1312, Age1313, Age1314, Age1316, Age1317, Age1318, Age1319, Age1320, Age1321, Age1322 These microsatellite data cannot be combined with data generated outside the Alaska Science Center without molecular size calibration between laboratories. Details and full citations for microsatellite loci are available at https://doi.org/10.5066/P9GJEX8Y Author defined Allele scores (in base pairs) for each microsatellite locus. U.S. Geological Survey USGS ScienceBase Team Mailing and Physical

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