Gravley, Megan C. (ORCID: 0000-0002-4947-0236) Pierson, Barabara J. (ORCID: 0000-0001-8233-874X) Sage, G. Kevin (ORCID: 0000-0003-1431-2286) Ramey, Andrew M. (ORCID: 0000-0002-3601-8400) Talbot, Sandra L. (ORCID: 0000-0002-3312-7214) 20191220 tabular digital data Anchorage, Alaska U.S. Geological Survey, Alaska Science Center Suggested Citation: Gravley, M.C., Pierson, B.J., Sage, G.K., Ramey, A.M., Talbot, S.L., 2019, DNA microsatellite markers for Northern fulmar (Fulmaris glacialis) and cross-species amplification in select seabird species: U.S. Geological Survey data release, https://doi.org/10.5066/P9KWA9VZ https://doi.org/10.5066/P9KWA9VZ This data set is comprised of one table with sampling information and allele sizes for 69 individual Northern Fulmars (Fulmarus glacialis) from Chagulak Island, Alaska and Hafnarey Island, Iceland. Fulmar allele sizes are provided for 10 previously published and 26 novel microsatellite loci. Additionally, allele sizes for the 26 microsatellite loci developed in this study are included for Arctic Terns (Sterna paradisaea, n=4), Common Murres (Uria aalge, n=6), Crested Auklets (Aethia cristatella, n=6), Fork-tailed Storm Petrels (Oceanodroma furcata, n=5), Marbled Murrelets (Brachyramphus marmoratus, n=5), Common Gulls (Larus canus, n=5), Pelagic cormorants (Phalacrocorax pelagicus, n=5), and Thick-billed Murres (Uria lomvia, n=5) from various locations throughout Alaska. Prior cross-species screens and classical microsatellite marker development specifically in Northern Fulmar, failed to provide a multilocus marker suite adequate for use in population genetics studies. 1991 2015 observed Complete None planned The sampling area includes Alaska from Attu Island in the southwest to Yakutat in the east northward to the Beaufort Sea. Additional samples originated from Hafnarey Island in the Breidi Fjord of Iceland. -180.0000 -10.5469 72.1818 50.5134 USGS Metadata Identifier USGS:ASC243 ISO 19115 Topic Category Biota Environment NASA GCMD Earth Science Thesaurus Animals/Vertebrates Birds Waders/Gulls/Auks and Allies Albatrosses/Petrels and Allies USGS CSA Biocomplexity Thesaurus Birds Population genetics Genetic variance Genetic diversity USGS Thesaurus Life Sciences Genetics DNA sequencing Polymerase chain reaction Systematics and taxonomy MeSH-National Library of Medicine Animals Charadriiformes Alleles Microsatellite Repeats Birds Arctic Regions Genetics, Population None Fulmarus glacialis Northern fulmar USGS Geographic Names Information System (GNIS) Alaska NGA GEOnet Names Server Iceland None Aethia cristatella Brachyramphus marmoratus maroratum Fulmarus glacialis Larus canus Oceanodroma furcata Phalacrocorax pelagicus Sterna paradisaea Uria aalge ITIS Integrated Taxonomic Information System Unknown online database online ITIS-North America Taxonomic details retrieved August 21, 2022, from the Integrated Taxonomic Information System online database https://www.itis.gov https://doi.org/10.5066/F7KH0KBK Species were identified by skilled observers in the field based on general appearance, plumage, behavior, and vocalization. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Aves Order Charadriiformes Family Alcidae Genus Aethia Species Aethia cristatella Crested Auklet TSN: 177019 Genus Uria Species Uria aalge Common Murre TSN: 176974 Genus Brachyramphus Species Brachyramphus marmoratus Marbled Murrelet TSN: 176996 Family Laridae Genus Larus Species Larus canus Mew Gull TSN: 176832 Genus Sterna Species Sterna paradisaea Arctic Tern TSN: 176890 Order Procellariiformes Family Procellariidae Genus Fulmarus Species Fulmarus glacialis Northern Fulmar TSN: 174536 Family Hydrobatidae Subfamily Hydrobatinae Genus Oceanodroma Species Oceanodroma furcata Fork-tailed Storm-Petrel TSN: 174625 Order Suliformes Family Phalacrocoracidae Genus Phalacrocorax Species Phalacrocorax pelagicus Pelagic Cormorant TSN: 174725 No access constraints. No use constraints. We request that the suggested citation of this USGS data release be included in any publications that reference or utilize these data. U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov Gravley, M.C. Sage, G.K. Ramey, A.M. Hatch, S.A. Gill, V.A. Rearick-Whitney, J.R. Petersen, A. Talbot, S.L. 2019 journal article Genes and Genomics 41(9):1015-1026 online Springer Nature Gravley, M.C., Sage, G.K., Ramey, A.M., Hatch, S.A., Gill, V.A., Rearick-Whitney, J.R., Petersen, A., Talbot, S.L., 2019. Development and characterization of polymorphic microsatellite markers in Northern Fulmar, Fulmarus glacialis (Procellariiformes), and cross-species amplification in eight other seabirds. Genes and Genomics 41(9):1015-1026, https://doi.org/10.1007/s13258-019-00819-5 https://doi.org/10.1007/s13258-019-00819-5 Sampling information provided by collectors was cross-checked and verified to match the accompanying data. Sampling locations were verified with collectors, labeled, and cross-referenced to the Geographic Names Information Service (GNIS) or NGA GEOnet Names Server when possible. Quality control (QC) included a minimum of 10% re-extraction and PCR replication for each locus and all laboratory techniques were performed using sterile procedures. All duplicate characterizations of the same individual resulted in concordant data. GenBank accession numbers were cross-referenced to the appropriate individual samples as recorded. Species identification by collectors were informally verified by confirming genetic "signatures" (e.g. microsatellite size or DNA sequence) fell within expected values for that species. Field sex was not confirmed with a laboratory analysis. For microsatellite loci, reported allele sizes were reviewed to confirm they conform to expected distributions (e.g. dinucleotide repeats with 1 base pair differences were reviewed on gel images by laboratory personnel, gaps in allele sizes were reviewed, and single occurrences of an allele size were scrutinized). Any inconsistencies in allele scores were resolved with additional replicates. Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004). Blank cells are described for each attribute. Attribute values of "np" (no product) indicate that a sample did not amplify or no PCR product was detected. Unknown Unknown document paper 1996 observed MEL Tissue Preservation Buffer Laboratory Technique Citation: Tissue Preservation Buffer. This buffer is often used to preserve non-blood tissue samples collected for DNA analysis at ambient temperatures until DNA extraction can occur. Current and past personnel of the Alaska Science Center as well as many associates familiar with the buffer are unsure of its origins, though its use at the Alaska Science Center for tissue preservation is known from at least 1996. Its formulation is (4.00 M Urea, 0.2 M NaCl, 10 mM EDTA, 0.5% N-Lauroyl-Sarcosine, 100 mM Tris-HCl, pH 8.0) stored at room temperature. National Center for Biotechnology Information (NCBI) Unknown database https://www.ncbi.nlm.nih.gov/genbank digital database file 2019 observed NCBI GenBank Genetic Sequence Database: microsatellite locus sequences are archived and publicly available through this database. DNA was extracted by a modified salt extraction technique (Medrano et al 1990; Sonsthagen et al 2004). A suite of microsatellite loci was chosen for testing. Polymerase chain reaction (PCR) amplification and scoring of microsatellite loci followed Sonsthagen et al 2004 except the thermocycler parameters consisted of a 2 min denaturation at 94 °C, followed 40 cycles at 94°C for 15 seconds, 50°C for 15 seconds and 72 °C for 30 seconds with a final extension at 72 °C for 30 min. Microsatellite alleles were resolved on a 25 cm 6% polyacrylamide gel using a LI-COR (IR 4200) automated DNA sequencer. For quality control purposes, we extracted, amplified, and sequenced, 10% of the samples in duplicate. Next generation shotgun sequencing data were collected on a 454 Life Sciences GS-Jr FLX (Roche, Branford CT) platform following a protocol similar to that reported in Sonsthagen et al 2013. A GS FLX Titanium Rapid Library MID Adaptor was ligated following nebulization. Unknown Next-generation sequencing reads used for microsatellite development were assembled into contigs using GS De Novo Assembler, Version 3.0 (Roche, Branford, CT). Contigs and unassembled reads were searched using Msatcommander 0.8.2 (Faircloth 2008) for microsatellites with ≥ 8 dinucleotide motifs. Faircloth 2008 Unknown Initial microsatellite allele sizes were determined by scoring based on PCR product size relative to an M13 DNA sequence ladder using Gene Profiler 4.05 (Scanalytics, Inc., Fairfax, VA). Subsequent gels included these known size individuals from which relative sizes of unknown individuals were assigned using Gene Profiler 4.05. For this reason, these microsatellite data cannot be combined with data generated outside the USGS Alaska Science Center without molecular size calibration between laboratories. Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004). Unknown Sequences of novel microsatellite loci developed in this study were submitted to the NCBI Genbank publicly available genetic sequence data database. Unknown NCBI GenBank LITERATURE CITED: Faircloth, B.C., 2008. MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Molecular Ecology Resources 8:92-94, https://doi.org/10.1111/j.1471-8286.2007.01884.x Handel, C.M., Pajot, L.M., Talbot, S.L., Sage, G.K., 2006. Use of buccal swabs for sampling DNA from nestling and adult birds. Wildlife Society Bulletin 34:1094-1100, https://doi.org/10.2193/0091-7648(2006)34[1094:UOBSFS]2.0.CO;2 Longmire, J.L., Lewis, A.K., Brown, N.C., Buckingham, J.M., Clark, L.M., Jones, M.D., Meincke, L.J., Meyne, J., Ratliff, R.L., Ray, F.A., Wagner, R.P., Moyzis, R.K., 1988. Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae. Genomics 2(1):14-24, https://doi.org/10.1016/0888-7543(88)90104-8 Medrano, J.F., Aasen, E., Sharrow, L., 1990. DNA extraction from nucleated red blood cells. Biotechniques 8(1):43 Sonsthagen, S.A., Talbot, S.L., White, C.M., 2004. Gene flow and genetic characterization of Northern Goshawks breeding in Utah. Condor 106(4):826–836, https://www.jstor.org/stable/3247787 Sonsthagen, S.A., Sage, G.K., Fowler, M.C., Hope, A.G., Cook, J.A., Talbot, S.L., 2013. Development and characterization of 21 polymorphic microsatellite markers for the barren-ground shrew, Sorex ugyunak (Mammalia: Sorcidae), through next-generation sequencing, and cross-species amplification in the masked shrew, S. cinereus. Conservation Genetics Resources 5(2):315–318, https://doi.org/10.1007/s12686-012-9792-5 van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P., 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4(3):535-538, https://doi.org/10.1111/j.1471-8286.2004.00684.x Unknown Sampling locations have been described by corresponding entries in the Geographic Names Information System (GNIS) or NGIA GeoNames Names Server when possible. Point 0.001 0.001 Decimal degrees World Geodetic System of 1984 (WGS84) World Geodetic System of 1984 (WGS84) 6378137 298.257223563 northernFulmar_microsat_alaskaIceland_gravley.csv Table with sample identifying information, collection data, and allele scores (in base pairs) for 36 autosomal microsatellite loci. Presented in a Comma Separated Value (CSV) formatted table. Locus names and order of presentation: Dc16, De7, 12H8, Fugl26, Fugl51, FuglDe11, Hbau4, Paequ3, Paequ4, Ruff50, Fgl001, Fgl002, Fgl003, Fgl004, Fgl005, Fgl006, Fgl007, Fgl008, Fgl009, Fgl010, Fgl011, Fgl012, Fgl013, Fgl014, Fgl015, Fgl016, Fgl017, Fgl018, Fgl019, Fgl020, Fgl021, Fgl022, Fgl023, Fgl024, Fgl025, Fgl026. Author defined Sample_ID USGS Alaska Science Center, Molecular Ecology Laboratory (MEL) alphanumeric label that uniquely identifies each sample. Author defined Unique identifier; an alphanumeric code for each individual sample in this dataset. Species The Scientific name of the seabird species of each sample. Author defined Fulmarus glacialis Scientific name of Northern Fulmar. Author defined Sterna paradisaea Scientific name of Arctic Tern. Author defined Uria aalge Scientific name of Common Murre. Author defined Aethia cristatella Scientific name of Crested Auklet. Author defined Oceanodroma furcata Scientific name of Fork-tailed Storm Petrel. Author defined Brachyramphus marmoratus Scientific name of Marbled Murrelet. Author defined Larus canus Scientific name of Common Gull. Author defined Phalacrocorax pelagicus Scientific name of Pelagic Cormorant. Author defined Uria lomvia Scientific name of Thick-billed Murre. Author defined Band_ID Unique field identification code, used to mark individual birds in the field. Five-digit numbers indicate a metal leg band issued by the USGS Bird Banding Laboratory, Patuxent Wildlife Research Center, Patuxent, Maryland. Six-digit numbers indicate the individual was banded and information maintained by the Iceland Bird Banding Office. Blank cells indicate that the bird was not banded. Author defined Unique field identification marker code. Sex The sex of the sampled bird, as reported to the laboratory by the source providing the DNA or DNA source tissue to the laboratory. Blank cells indicate information was not provided. Author defined M Male Author defined F Female Author defined Collection_location Place names of sample collection sites. Author defined Chagulak Island Samples collected on or around Chagulak Island, Alaska (approx 52.56N, 171.16W) Geographic Names Information System (GNIS) Hafnarey Island (Iceland) Samples collected on or around Hafnarey Island in the Breidi Fjord, northwest Iceland (approx 65.378N,22.919W). NGIA GeoNames Kokechik Bay Samples collected in or around Kokechik Bay, Alaska (approx 61.7N, 166.0W) Geographic Names Information System (GNIS) Anchorage Samples collected in or around Anchorage, Alaska (approx 61.2N, 149.9W) Geographic Names Information System (GNIS) Beaufort Sea Samples collected in or near the Beaufort Sea, Alaska Geographic Names Information System (GNIS) Matanuska-Susitna Valley Samples collected in or around the Matanuska Valley and Susitna Valley, Alaska (approx 61.3N, 150.2W) Geographic Names Information System (GNIS) Cape Espenberg Samples collected on or around Cape Espenberg, Alaska (approx 66.6N, 164.1W) Geographic Names Information System (GNIS) Prince William Sound Samples collected in or around Prince William Sound, Alaska (approx 60.5N, 147.2W) Geographic Names Information System (GNIS) Pribilof Islands Samples collected in or around the Pribilof Islands, Alaska (approx 56.8N, 170.0W) Geographic Names Information System (GNIS) Bering Sea Samples collected in or around the Bering Sea, Alaska Geographic Names Information System (GNIS) Unalga Pass Samples collected in or around Unalga Pass, Alaska (approx N53.95, 166.2W) Geographic Names Information System (GNIS) Dutch Harbor Samples collected in or around Dutch Harbor, Alaska (approx 53.9N, 166.6W) Geographic Names Information System (GNIS) Innoko NWR Samples collected in or around the Innoko National Wildlife Refuge, Alaska (approx 63.2N, 158.7W) Geographic Names Information System (GNIS) Alaska Peninsula NWR Samples collected on or around the Alaska Peninsula National Wildlife Refuge, Alaska (approx 57.2N, 156.8W) Geographic Names Information System (GNIS) Sitka Samples collected in or around Sitka, Alaska (approx 57.05N, 135.35W) Geographic Names Information System (GNIS) Cape Uganik Samples collected in or around Cape Uganik, Alaska (approx 58.0N, 153.5W) Geographic Names Information System (GNIS) Kodiak Samples collected on or around Kodiak Island, Alaska (approx 57.5N, 153.5W) Geographic Names Information System (GNIS) Anchor Point Samples collected in or around Anchor Point on the Kenai Peninsula, Alaska (approx 59.8N, 151.9W) Geographic Names Information System (GNIS) Yakutat Samples collected in or around Yakutat, Alaska (approx 59.6N, 139.7W) Geographic Names Information System (GNIS) Alaska Peninsula NWR Samples collected in or around the Alaska Peninsula National Wildlife Refuge, Alaska (approx 56.3N, 159.2W) Geographic Names Information System (GNIS) Attu Samples collected on or around Attu Island, Alaska (approx 52.9N, 172.6E) Geographic Names Information System (GNIS) Middleton Island Samples collected on or around Middleton Island, Alaska (approx 59.45N, 146.31W) Geographic Names Information System (GNIS) St. George Island Samples collected on or around Saint George Island, Alaska (approx 56.6N, 169.6W) Geographic Names Information System (GNIS) Latitude Latitude of field collection site. Blank cells indicate information is not available. Author defined 52.566 65.378 Decimal degrees (WGS84) Longitude Longitude of field collection site. Blank cells indicate information is not available. Author defined -171.161 -22.919 Decimal degrees (WGS84) Field_collection_start The date the sample was collected in the field. If the specific date is not known, then this is the start date of the field collection effort. Blank cells indicate a specific date or date range is not available. Author defined 8/4/1991 5/24/2015 Date (MM/DD/YYYY) Field_collection_end The date the sample was collected in the field. If the specific date is not known, then this is the start date of the field collection effort. Blank cells indicate a specific date or date range is not available. Author defined 8/4/1991 5/24/2015 Date (MM/DD/YYYY) Field_collection_misc Samples obtained opportunistically sometimes have incomplete collection information preventing assignment of a specific collection date. Less precise dates are given here when available. Blank cells indicate a specific date or date range has been given in the Field_collection_start and/or Field_collection_end, or if all three are blank, indicates sampling date information is unavailable. Author defined 2008 This individual was labelled as collected in 2008 with no more specific date or season listed. Author defined Spring-summer This individual was labelled as collected in Spring-summer with a note indicating it was from an unknown year. Author defined Tissue_type The DNA source material. Author defined Blood DNA for this sample was extracted from whole blood. Author defined Tissue DNA for this sample was extracted from muscle tissue. Author defined Storage_medium Blood or tissue samples are often received in a storage medium. Blank cells indicate the storage medium is unknown. Author defined Filter paper DNA extracted from blood droplets fixed on filter paper with alcohol and dried. Author defined LMB DNA for this sample was extracted from whole blood preserved in Longmire buffer. Longmire et al 1988 TPB DNA for this sample was extracted from tissue preserved in 4 M Urea Tissue Preservation Buffer. MEL Tissue Preservation Buffer Dc16_allele1 through Fgl026_allele_2 These 72 columns represent 36 autosomal microsatellite loci. Each microsatellite locus genotype is represented by two adjacent columns (i.e., allele1 and allele2) each containing one allele score. NP indicates resulting PCR product was absent or of insufficient quality to enable analysis. Blank cells indicate data were not collected for the sample. Locus names and order of presentation: Dc16, De7, 12H8, Fugl26, Fugl51, FuglDe11, Hbau4, Paequ3, Paequ4, Ruff50, Fgl001, Fgl002, Fgl003,Fgl004, Fgl005, Fgl006, Fgl007, Fgl008, Fgl009, Fgl010, Fgl011, Fgl012, Fgl013, Fgl014, Fgl015, Fgl016, Fgl017, Fgl018, Fgl019, Fgl020, Fgl021, Fgl022, Fgl023, Fgl024, Fgl025, Fgl026. These microsatellite data cannot be combined with data generated outside the Alaska Science Center without molecular size calibration between laboratories. Details and full citations for microsatellite loci are available at https://doi.org/10.5066/P9GJEX8Y Author defined Allele scores (in base pairs) for each microsatellite locus. U.S. Geological Survey USGS ScienceBase Team Mailing and Physical

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Denver Colorado 80225 USA 1-888-275-8747 sciencebase@usgs.gov The U.S. Geological Survey, Alaska Science Center is the authoritative source of these data, distributed by ScienceBase (a USGS Trusted Digital Repository). Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data for other purposes or on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. CSV Tabular data in CSV format; FGDC metadata in XML and HTML formats. https://doi.org/10.5066/P9KWA9VZ None 20251203 U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata (CSDGM) FGDC-STD-001.1-1999