Sonsthagen, Sarah A. (ORCID: 0000-0001-6215-5874) Pierson, Barbara J. (ORCID: 0000-0001-8233-874X) 20171116 tabular digital data Peregrine Falcon (Falco peregrinus) mtDNA and Microsatellite Genetic Data, Alaska, Canada and Russia, 1880-2012 ver 2.0, June 2022 Anchorage, Alaska U.S. Geological Survey, Alaska Science Center Suggested Citation: Sonsthagen, S.A., Pierson, B.J., 2017. Peregrine Falcon (Falco peregrinus) mtDNA and microsatellite genetic data, Alaska, Canada and Russia, 1880-2012 (ver 2.0, June 2022): U.S. Geological Survey data release, https://doi.org/10.5066/F7F18WV0 https://doi.org/10.5066/F7F18WV0 This data set provides sample information, microsatellite genotype, and NCBI accession numbers for mitochondrial DNA sequences of peregrine falcons (Falco peregrinus) from North America and Russia. To examine spatial and temporal genetic structure, inter-individual relationships, and assess subspecific taxonomic relationships of peregrine falcons. 2012 2015 observed Complete None planned northwestern North America and Commander Island Group, Russia 166.628198 -109.5 70.4461111 48.3720442 USGS Metadata Identifier USGS:ASC143 ISO 19115 Topic Category Biota Environment NASA GCMD Earth Science Keyword Thesaurus Animals/Vertebrates Birds Eagles/Falcons/Hawks and Allies USGS CSA Biocomplexity Thesaurus Birds of prey Genetic Diversity Biodiversity Gene Flow Population Genetics Genetic Variance USGS Thesaurus Wildlife Terrestrial ecosystems Genetics Polymerase chain reaction MeSH-National Library of Medicine DNA Alleles Genetic Markers Microsatellite Repeats Genotype None Raptors Peregrine Falcon Falco peregrinus USGS Geographic Names Information System (GNIS) Alaska Washington NGA GEOnet Names Server Canada Russia Alberta Saskatchewan British Columbia Nunavut Northwest Territories None Animals Birds Raptors ITIS Integrated Taxonomic Information System Unknown online database online ITIS-North America Taxonomic details retrieved August 21, 2022, from the Integrated Taxonomic Information System online database https://www.itis.gov https://doi.org/10.5066/F7KH0KBK Samples were provided by scientists competent to identify Falco peregrinus to the species level. DNA data generated in this study would have identified any non-Falco peregrinus samples. Sub-species designations were based on currently recognized geographic bounds of each, with samples in contact zones labeled as possibly of either species. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Aves Order Falconiformes Family Falconidae Subfamily Falconinae Genus Falco Species Falco peregrinus Peregrine Falcon TSN: 175604 Subspecies Falco peregrinus anatum American Peregrine Falcon TSN: 175605 Subspecies Falco peregrinus pealei TSN: 175606 Subspecies Falco peregrinus tundrius Arctic Peregrine Falcon TSN: 175608 No access constraints. No use constraints. We request that the suggested citation of this USGS data release be included in any publications that reference or utilize these data. U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov U.S. Geological Survey, Alaska Science Center, Anchorage, Alaska; Fairbanks Fish and Wildlife Field Office, U. S. Fish and Wildlife Service, Fairbanks, Alaska; Alaska Maritime National Wildlife Refuge. U. S. Fish and Wildlife Service, Homer, Alaska, USA; Los Alamos National Laboratory, Los Alamos, New Mexico; Yukon-Charley Rivers National Preserve and Gates of the Arctic National Park and Preserve, National Park Service, Fairbanks, Alaska; Migratory Bird Management, U. S. Fish and Wildlife Service, Juneau, Alaska; Alaska Regional Office, National Park Service, Anchorage, Alaska; Kenai Fjords National Park, National Park Service, Seward, Alaska; Falcon Research Group, Bow, Washington, USA; Department of Plant and Wildlife Sciences and Monte L. Bean Life Science Museum, Brigham Young University, Provo, Utah, USA Talbot, S.L. Sage, G.K. Sonsthagen, S.A. Gravley, M.C. Swem, T. Williams, J.C. Longmire, J.L. Ambrose, S. Flamme, M.J. Lewis, S.B. Phillips, L. Anderson, C. White, C.M. 2017 journal article PLoS One 12(11):e0188185 online PLoS Talbot, S.L., Sage, G.K., Sonsthagen, S.A., Gravley, M.C., Swem, T., Williams, J.C., Longmire, J.L., Ambrose, S., Flamme, M.J., Lewis, S.B., Phillips, L., Anderson, C., White, C.M., 2017. Intraspecific evolutionary relationships among peregrine falcons in western North American high latitudes. PLoS One 12(11):e0188185, https://doi.org/10.1371/journal.pone.0188185 https://doi.org/10.1371/journal.pone.0188185 Sonsthagen, S.A. Williams, J.C. Drew, G.S. White, C.M. Sage, G.K. Talbot, S.L. 2017 journal article Ecology and Evolution 7(1):107-114 online Wiley Sonsthagen, S.A., Williams, J.C., Drew, G.S., White, C.M., Sage, G.K., Talbot, S.L., 2017. Legacy or colonization? Posteruption establishment of peregrine falcons (Falco peregrinus) on a volcanically active subarctic island. Ecology and Evolution 7(1):107-114, https://doi.org/10.1002/ece3.2631 https://doi.org/10.1002/ece3.2631 Sonsthagen, S.A. Swem, T. Ambrose, S. Flamme, M.J. White, C.M. Sage, G.K. Talbot, S.L. 2022 journal article Ibis 164(4):1265-1272 online Wiley Sonsthagen, S.A., Swem, T., Ambrose, S., Flamme, M.J., White, C.M., Sage, G.K., Talbot, S.L., 2022. DDT-induced decline influenced genetic diversity in naturally recovered Peregrine falcons (Falco peregrinus) nesting within the Alaska Arctic and eastern interior. Ibis 164(4):1265-1272, https://doi.org/10.1111/ibi.13095 https://doi.org/10.1111/ibi.13095 Identifying information about samples were cross-checked with original field data. Polymerase chain reaction (PCR) amplification and electrophoresis followed protocols described in Sonsthagen et al 2004. Quality control (QC) included a minimum of 10% re-extraction and PCR replication for each locus and all laboratory techniques were performed using sterile procedures. All duplicate characterizations of the same individual resulted in concordant data. GenBank accession numbers were cross-referenced to the appropriate individual samples as recorded. For microsatellite loci, reported allele sizes were reviewed to confirm that they conform to expected distributions (e.g. dinucleotide repeats with 1 base pair differences were reviewed on gel images by laboratory personnel, gaps in allele sizes were reviewed, and single occurrences of an allele size were scrutinized). No inconsistencies in genotype scores were observed between replicates. Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004). Data omissions for particular entities are explained in the Entity and Attribute definitions. Blank cells are described for each attribute. Subspecies of a sample is based upon currently recognized distribution maps and location of sampling unless field sampling information precluded this assignment (e.g. sampling occurred near a recognized subspecies boundary). In these instances (Porcupine River and San Juan Islands), the two possible subspecies are listed. Known related individuals (from field records) are noted. LAB: DNA extractions were performed as described in Talbot et al (2017). Polymerase chain reaction (PCR) amplification and scoring of microsatellites followed Talbot et al (2011) using primer pairs as published by Nesje et al (2000). Sequencing protocols followed Talbot et al (2011) and White et al (2013); for some feather and museum samples that did not produce product using these protocols, a set of internal primers were designed to amplify 2 smaller, overlapping portions of the target sequence and are described in Talbot et al (2017). Initial microsatellite allele sizes were determined by scoring based on PCR product size relative to an M13 DNA sequence ladder using Gene Profiler 4.05 (Scanalytics, Inc., Fairfax, VA). Subsequent gels included these known size individuals from which relative sizes of unknown individuals were assigned using Gene Profiler 4.05. For this reason, these microsatellite data cannot be combined with data generated outside the USGS Alaska Science Center without molecular size calibration between laboratories. MtDNA sequences from opposite strands were reconciled using Sequencher 4.1.2 (Gene Codes Corporation, Ann Arbor, MI). Tests for null alleles and allelic dropout were implemented in MicroChecker (van Oosterhout et al 2004). MtDNA sequences were analyzed using eSeq ver.2.0 (LI-COR, Lincoln, NE) imaging software with visual proofing and aligned with each other using AlignIR ver. 2.0. Unknown Sequences of novel microsatellite loci developed in this study were submitted to the NCBI Genbank publicly available genetic sequence data database. Unknown NCBI GenBank LITERATURE CITED: Nesje, M., Roed, K.H., Lifjeld, J.T., Lindberg, P., Steen, O.F., 2000. Genetic relationships in the peregrine falcon (Falco peregrinus) analysed by microsatellite DNA markers. Molecular Ecology 9(1):53-60, https://doi.org/10.1046/j.1365-294x.2000.00834.x Shank, C.C., Bromley, R.G., Poole, K.G., 1993. Increase in breeding population of tundra Peregrine Falcons in the central Canadian arctic. Wilson Bulletin 105:188-190, https://www.jstor.org/stable/4163260 Talbot, S.L, Palmer, A.G., Sage, G.K., Sonsthagen, S.A., Swem, T., Brimm, D.J., White, C.M., 2011. Lack of genetic polymorphism among peregrine falcons Falco peregrinus of Fiji. Journal of Avian Biology 42(5):415–428, https://doi.org/10.1111/j.1600-048X.2011.05280.x Talbot, S.L., Sage, G.K., Sonsthagen, S.A., Gravley, M.C., Swem, T., Williams, J.C., Longmire, J.L., Ambrose, S., Flamme, M.J., Lewis, S.B., Phillips, L., Anderson, C., White, C.M., 2017. Intraspecific evolutionary relationships among peregrine falcons in western North American high latitudes. PLoS One 12(11):e0188185, https://doi.org/10.1371/journal.pone.0188185 van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., Shipley, P., 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4(3):535-538, https://doi.org/10.1111/j.1471-8286.2004.00684.x White, C.M., Sonsthagen, S.A., Sage, G.K., Anderson, C., Talbot, S.L., 2013. Genetic relationships among some subspecies of the Peregrine Falcon (Falco peregrinus L.), inferred from mitochondrial DNA control-region sequences. Auk 130(1):78–87, https://doi.org/10.1525/auk.2012.11173 Unknown General sampling locations have been described by corresponding entries in the USGS Geographic Names Information System (GNIS) when possible. peregrineFalcon_microsat_talbot.csv Table with sample identifying information, collection data, and genotype numbers for 12 autosomal microsatellite loci (NVHfp05, NVHfp13, NVHfp31, NVHfp46-1, NVH54, NVHfp79-1, NVHfp79-4, NVHfp82-2, NVHfp86-2, NVHfp89, NVHfp92-1, NVHfp107). Genotype numbers (nucleotide base pairs) were determined by scoring based on allele size relative to an M13 DNA sequence ladder and to samples established as size standards that were run on each gel. Presented in a Comma Separated Value (CSV) formatted table. Author defined Version The data package version that was current at the time each record was released. See "versionHistory.txt" included with this data package for additional details. Author defined 1 Data were originally released with version 1.0 of this data release. Author defined 2 Data were originally released with version 2.0 of this data release. Author defined Sample_ID USGS Alaska Science Center, Molecular Ecology Laboratory (MEL) alphanumeric label that uniquely identifies each sample. Blank cells indicate samples that are not archived at MEL. Author defined Unique identifier; an alphanumeric code for each individual sample in this dataset. Specimen_voucher Description of the museum voucher number. Alpha characters denote museum code where specimen is archived. Alpha characters combine with numeric characters uniquely identify a sample. Blank cells indicate samples that are not museum vouchers. Author defined BYU Monte L. Bean Life Museum Author defined CMW Monte L. Bean Life Museum Author defined MSB Museum of Southwest Biology Author defined MVZ Museum of Vertebrate Zoology Author defined UAM Museum of the North Author defined UMNH Utah Museum of Natural History Author defined USNM National Museum of Natural History Author defined Sampling_Location This is the geographic area at which the sample was collected. Blank cells indicate location information is available from museum database. Author defined Tanana River This refers to the geographic location of the Tanana River, Alaska. Geographic Names Information System (GNIS) Yukon River This refers to the geographic location of the Yukon River, Alaska. Geographic Names Information System (GNIS) Yukon-Charley This refers to the geographic location of the Yukon-Charley Rivers National Preserve, Alaska. Geographic Names Information System (GNIS) Porcupine River This refers to the geographic location of the Porcupine River, Alaska. Geographic Names Information System (GNIS) Colville River This refers to the geographic location of the Colville River, Alaska. Geographic Names Information System (GNIS) Sagavanirktok River This refers to the geographic location of the Sagavanirktok River, Alaska. Geographic Names Information System (GNIS) San Juan Islands This refers to the geographic location of the San Juan Islands, Washington. Geographic Names Information System (GNIS) Alexander Archipelago This refers to the geographic location of the Alexander Archipelago, Alaska. Geographic Names Information System (GNIS) Kasatochi Island This refers to the geographic location of Kasatochi Island, Alaska. Geographic Names Information System (GNIS) Amat Island This refers to the geographic location of Amatignak Island, Alaska. Geographic Names Information System (GNIS) Amchitka Island This refers to the geographic location of the Amchitka Island, Alaska. Geographic Names Information System (GNIS) Buldir Island This refers to the geographic location of Buldir Island, Alaska. Geographic Names Information System (GNIS) Attu Island This refers to the geographic location of Attu Island, Alaska. Geographic Names Information System (GNIS) Chatham Island This refers to the geographic location of the Chatham Island, Alaska. Geographic Names Information System (GNIS) Cheval Island This refers to the geographic location of Cheval Island, Alaska. Geographic Names Information System (GNIS) Granite Island This refers to the geographic location of Granite Island, Alaska. Geographic Names Information System (GNIS) Yalik Point This refers to the geographic location of Yalik Point, Alaska. Geographic Names Information System (GNIS) Aialik Cape This refers to the geographic location of Aialik Cape, Alaska. Geographic Names Information System (GNIS) Kenai Fjords This refers to the geographic location of Kenai Fjords National Park, Alaska. Geographic Names Information System (GNIS) Palmer This refers to the geographic vicinity of Palmer, Alaska. Geographic Names Information System (GNIS) Southcentral This refers to a large area encompassing Cook Inlet, the Matanuska-Susitna Valley, the Kenai Peninsula, Prince William Sound, and Copper River Valley. More specific location information is unavailable. Author defined Kodiak Island This refers to the geographic location of Kodiak Island, Alaska. Geographic Names Information System (GNIS) Mackenzie River Valley This refers to a geographic location along the Mackenzie River, Northwest Territories, Canada. NGA GEOnet Names Server (GNS) Horton River This refers to a geographic location along the Horton River, Northwest Territories, Canada. NGA GEOnet Names Server (GNS) northern Alberta This indicates a non-specific location in northern Alberta, Canada in the general vicinity of Lake Athabasca. Author defined Lake Athabasca This refers to a geographic location near Lake Athabasca, Canada. NGA GEOnet Names Server (GNS) Hope Bay This refers to a geographic area known as Hope Bay, Nunavut, Canada described as, "situated on the coast of the Arctic Ocean in the central Northwest Territories (68.16 N, 106.25 W)" in Shank et al. 1993. Increase in breeding population of tundra Peregrine Falcons in the central Canadian arctic. Wilson Bull. 105:188-190. Note: Nunavut was established in 1999, after original manuscript referenced the location in NWT. Author defined Langara Island This refers to a geographic location on Langara Island, British Columbia, Canada. NGA GEOnet Names Server (GNS) Haida Gwaii This refers to a geographic location on Haida Gwaii, British Columbia, Canada (formerly known as the Queen Charlotte Islands archipelago). NGA GEOnet Names Server (GNS) Commander Islands This refers to a geographic location on the Commander Islands, Kamchatka Territory, Russia. NGA GEOnet Names Server (GNS) State/Territory/Province This indicates the state, territory or province of the sampling location. Blank cells indicate location information is available from museum database. Author defined Alaska This indicates a location in the state of Alaska, USA. Geographic Names Information System (GNIS) Washington This indicates a location in the state of Washington, USA. Geographic Names Information System (GNIS) Alberta This indicates a location in the province of Alberta, Canada. NGA GEOnet Names Server (GNS) Saskatchewan This indicates a location in the province of Saskatchewan, Canada. NGA GEOnet Names Server (GNS) Northwest Territories This indicates a location in the Northwest Territories of Canada. NGA GEOnet Names Server (GNS) British Columbia This indicates a location in the province of British Columbia in Canada. NGA GEOnet Names Server (GNS) Nunavut This indicates a location in the territory of Nunavut, Canada. NGA GEOnet Names Server (GNS) Kamchatskiy Kray This indicates a location in the "krai" (territory) of Kamchatka, Russia. NGA GEOnet Names Server (GNS) Country This indicates the country of the sampling location. Blank cells indicate location information is available from museum database. Author defined USA This refers to the country of the United States of America. Geographic Names Information System (GNIS) Canada This refers to the country of Canada. NGA GEOnet Names Server (GNS) Russia This refers to the country of Russia. NGA GEOnet Names Server (GNS) Collection_Year This indicates the year the DNA source was sampled from the field. "1970s" indicates the decade, but not specific year of sampling; a specific year was unavailable for these samples. Blank cells indicate information is not available or is available from museum database. Author defined 1880 2012 Year (YYYY) 1970s A value of 1970s indicates the decade a sample was collected when no specific year was available. Author defined DNA_Source_Material This indicates the DNA source material upon receipt in the laboratory. Blank information indicates that source material was not recorded. Author defined DNA DNA for this sample was provided to the laboratory by other researchers. Author defined epithelial cells DNA for this sample was extracted from epithelial cells on a swab provided to the laboratory. Author defined Toe Pad Toe pad tissue sample collected by museum via tissue loan request. Author defined eggshell membrane DNA for this sample was extracted from eggshell membrane provided to the laboratory. Author defined whole blood DNA for this sample was extracted from whole blood provided to the laboratory. Author defined blood/LPB DNA for this sample was extracted from whole blood preserved in Longmire buffer (LMB; as referenced in Longmire, J.L., A.K. Lewis, N.C. Brown, J.M. Buckingham, L.M. Clark, M.D. Jones, L.J. Meincke, J. Meyne, R.L. Ratliff, F.A. Ray, R.P. Wagner, and R.K. Moyzis. 1988. Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae. Genomics 2:14–24). Author defined blood on filter paper DNA for this sample was extracted from whole blood on filter paper (fp). Author defined muscle DNA for this sample was extracted from muscle tissue provided to the laboratory. Author defined muscle/LPB DNA for this sample was extracted from muscle tissue preserved in Longmire buffer (LMB; as referenced in Longmire, J.L., A.K. Lewis, N.C. Brown, J.M. Buckingham, L.M. Clark, M.D. Jones, L.J. Meincke, J. Meyne, R.L. Ratliff, F.A. Ray, R.P. Wagner, and R.K. Moyzis. 1988. Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family Falconidae. Genomics 2:14–24). Author defined muscle DNA for this sample was extracted from a muscle sample provided to the laboratory. Author defined skin DNA for this sample was extracted from a prepared skin sample provided to the laboratory. Author defined feather DNA for this sample was extracted from a feather sample provided to the laboratory. Author defined talon DNA for this sample was extracted from a talon provided to the laboratory. Author defined bone DNA for this sample was extracted from a bone sample provided to the laboratory. Author defined Subspecies This indicates the subspecies of a sample based upon currently recognized distribution maps and location of sampling. Blank fields indicate subspecies information is available from museum database. Author defined anatum This indicates the sample is presumed to be the subspecies Falco peregrinus anatum. Author defined anatum/tundrius? Field sampling information precludes assignment of subspecies given geographic location between these two currently recognized subspecies. Author defined tundrius This indicates the sample is presumed to be the subspecies Falco peregrinus tundrius. Author defined anatum/pealei? Field sampling information precludes assignment of subspecies given geographic location between these two currently recognized subspecies. Author defined pealei This indicates the sample is presumed to be the subspecies Falco peregrinus pealei. Author defined Field_Sex This indicates the sex reported to the laboratory by the source providing the DNA or DNA source tissue to the laboratory. Blank cells indicate that sex information was not provided by source or is available from museum database. Author defined M Sample was identified as a male in the field. This information was provided to, but not generated by the laboratory. Author defined F Sample was identified as a female in the field. This information was provided to, but not generated by the laboratory. Author defined CHD_Sex This is the laboratory assigned sex based on the absence (male: ZZ) or presence (female: ZW) of the W-chromosome CHD PCR product. Blank cells indicate data were not collected. Talbot, S.L. et al. 2011. Lack of genetic polymorphism among peregrine falcons Falco peregrinus of Fiji. J. Avian. Biol. 42: 415-428. M DNA analyses resulted in a ZZ genotype indicating this sample is male. Author defined F DNA analyses resulted in a ZW genotype indicating this sample is female. Author defined Population_Acronym This is a 3-5 letter abbreviation indicating the geographic locale of a sample. Blank cells indicate that population acronym was not used in data analysis. Author defined TAN Samples collected in the general vicinity of the Tanana River, Alaska. Author defined YUK Samples collected in the general vicinity of the Yukon River, Alaska. Author defined POR Samples collected in the general vicinity of the Porcupine River, Alaska. Author defined LKAT Samples collected in the general vicinity of the Lake Athabasca, Canada. Author defined MACV Samples collected in the general vicinity of the Mackenzie River, Canada. Author defined HOPEB Samples collected in the general vicinity of Hope Bay, Nunavut, Canada. Author defined COLV Samples collected in the general vicinity of the Colville River, Alaska. Author defined SJI Samples collected in the general vicinity of the San Juan Islands, Washington. Author defined AARCH Samples collected in the general vicinity of the Alexander Archipelago, Alaska. Author defined ANDR Samples collected in the general vicinity of the Andreanof Islands, Alaska. Author defined RAT Samples collected in the general vicinity of the Rat Islands, Alaska. Author defined NEAR Samples collected in the general vicinity of the Near Islands, Alaska. Author defined COMM Samples collected in the general vicinity of the Commander Islands, Russia. Author defined KF Samples collected in the general vicinity of Kenai Fjords, Alaska. Author defined SCEN Samples collected in the general vicinity of Southcentral, Alaska. Author defined Known_Relationships Known related individuals are recorded here. Blank cells indicate that relationship information is not known. Author defined For any given Sample_ID, parents are listed under the Known_Relationships attribute as P=Sample_ID and offspring as F1=Sample_ID where the Sample_ID following the "=" is the parent or offspring, respectively, of the individual for which data is being provided. Associated_Manuscripts In some cases, individual sample data have been analyzed and published in multiple manuscripts. This attribute lists in which manuscript(s) a sample's data have been included and specifically which combination of data were included. Author defined A Indicates microsatellite data for this individual was used for analyses published in Sonsthagen et al 2017 Author defined B Indicates mtDNA control region and microsatellite data were used for analyses published in Talbot et al 2017 Author defined C Indicates only microsatellite data were used for analyses published in Talbot et al 2017 Author defined D Indicates only mtDNA data were used for analyses published in Talbot et al 2017 Author defined E Indicates mtDNA control region and microsatellite data were used for analyses published in Sonsthagen et al 2022 Author defined BE Indicates mtDNA control region and microsatellite data were used for analyses published in Talbot et al 2017 and Sonsthagen et al 2022 Author defined CE Indicates only microsatellite data for this individual was used for analyses published in Talbot et al 2017 and indicates mtDNA control region and microsatellite data were used for analyses published in Sonsthagen et al 2022 Author defined AB Indicates microsatellite data for this individual was used for analyses published in Sonsthagen et al 2017 and indicates mtDNA control region and microsatellite data were used for analyses published in Talbot et al 2017 Author defined AC Indicates microsatellite data for this individual was used for analyses published in Sonsthagen et al 2017 and indicates only microsatellite data were used for analyses published in Talbot et al 2017 Author defined AD Indicates microsatellite data for this individual was used for analyses published in Sonsthagen et al 2017 and indicates only mtDNA data were used for analyses published in Talbot et al 2017 Author defined mtDNA_CR1_Haplotype mtDNA haplotype assigned based on site substitutions at 27 sites in 559 base pairs sequenced for each individual as referenced in White et al. 2013. Representative individual sequences have been submitted to GenBank. Author defined CR2 CR48 GenBank Accession Number These haplotype assignments (CR2-CR48, non-sequential) follow White et al. 2013 Genbank_Accession_mtDNA_CR1 GenBank Accession Number - unique identifier for the mitochondrial DNA control region (CR) nucleotide sequence, assigned by the National Center for Biotechnology Information (NCBI). Blank cells indicate that sequence data from this locus were not collected for this sample. Author defined Unique identifier: GenBank accession number assigned by NCBI. This number may be used to retrieve detailed sample and sequence information from https://www.ncbi.nlm.nih.gov/ NVHfp05_Allele_1 through NVHfp107_Allele_2 These 24 columns represent 12 autosomal microsatellite loci. Each microsatellite locus genotype is represented by two adjacent columns (i.e., allele1 and allele2) each containing one allele score. Blank cells indicate data were not collected for the sample. Locus names and order of presentation: NVHfp05, NVHfp13, NVHfp31, NVHfp46-1, NVH54, NVHfp79-1, NVHfp79-4, NVHfp82-2, NVHfp86-2, NVHfp89, NVHfp92-1, NVHfp107 These microsatellite data cannot be combined with data generated outside the Alaska Science Center without molecular size calibration between laboratories. Author defined Allele scores (in base pairs) for each microsatellite locus. U.S. Geological Survey USGS ScienceBase Team Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata (CSDGM) FGDC-STD-001.1-1999