RT-QuIC Florescence Data from Experimental Soils Varying in Soil Texture

Stephanie J. Katircioglu Heather N. Inzalaco Allen Herbst Daniel J. Storm Stuart S. Lichtenberg Rodrigo Morales Reece McGinn Daniel P. Walsh Wendy C. Turner 20260401 RT-QuIC Florescence Data from Experimental Soils Varying in Soil Texture tabular digital data https://doi.org/10.5066/P13AJ49L We assessed the performance of the real-time quaking-induced conversion (RT-QuIC) assay under different soil textures. We combined sand, silt and clay in different proportions to create experimental soils representative of southwest Wisconsin, USA. Experimental soils were spiked with a brain homogenate from a chronic wasting disease positive white-tailed deer (to test assay sensitivity) or water (to test assay specificity). The dataset includes raw fluorescence data collected every 15 minutes for 48 hours. To understand the influence of soil texture on a prion detection assay 20220601 20251231 ground condition Complete None planned University of Wisconsin–Madison -89.4291 -89.4014 43.0735 43.0805 ISO 19115 Topic Category environment USGS Thesaurus soil sciences chronic wasting disease USGS Metadata Identifier USGS:6995c1afb66b01c0fd9b2afb No access constraints. Please see 'Distribution Information' for details. These data are marked with a Creative Commons Zero v1.0 Universal (CC0-1.0) public domain dedication and do not have any use constraints. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Wendy C. Turner USGS Wisconsin Cooperative Wildlife Research Unit mailing and physical

1630 Russell Laboratories

Madison WI 53706 608-263-6882 USGS Biothreats Funding USGS Wisconsin Cooperative Wildlife Research Unit USGS Montana Cooperative Wildlife Research Unit USGS National Wildlife Health Center Values are generated by a plate reader that is maintained according to the manufacturer's specifications. It is calibrated. The plates are run with positive and negative controls. Data appear logically consistent Data include fluorescent readings of each well of a 96-well plate at each 15-min reading for the duration of the assay. The methodology used to conduct the RT-QuIC assay are detailed in the associated manuscript. Data were generated by the Omega microplate reader (BMG Labtech, Cary, North Carolina, USA) in proprietary software (MARS version 4.01 R2) which generates .ruc files designed to comply with FDA 21 CFR Part 11 regulations. Data were extracted as .csv files within the proprietary software, and combined into a single .csv file for this release. Time-to-threshold data were calculated for each plate from the wells containing soil samples. This threshold was calculated by adding ten times the standard deviation of the raw fluorescence values from cycles 3-14 to the mean of raw fluorescence values from cycles 3-14. In the MARS software, we input the plate threshold value, and the software calculated the time to threshold for each well. We only report these thresholds for the soil samples. 20260209 master-datarelease-file.csv Comma Separated Value (CSV) file containing data. Producer Defined Plate-ID Label identifying plates to connect sample types to plate of origin. Producer Defined 1 84 Experimental_test Type of experimental test for each 96-well plate Producer Defined sensitivity Where soil samples were spiked with CWD positive deer brain Producer defined specificity Where soil samples were spiked with water Producer defined Sample_type The type of sample in each well of a 96-well plate Producer Defined positive control CWD positive deer brain Producer defined experimental soil experimental soil at the defined texture Producer defined buffer control buffer control Producer defined %_Clay The percentage of clay in the experimental soil Producer Defined NA Sample type is not soil Producer defined 5.0 50.0 %_Sand The percentage of sand in the experimental soil Producer Defined NA Sample type is not soil Producer defined 0 80 %_Silt The percentage of silt in the experimental soil Producer Defined NA Sample type is not soil Producer defined 2.0 95.0 Textural_class Soil texture based on United States Department of Agriculture soil classifications USDA NA Sample type is not soil Producer Defined Silt Loam Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Sandy Loam Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Silt Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Loam Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Sandy Clay Loam Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Silt loam Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Silty Clay Loam Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA Sandy Clay Described in detail: https://www.nrcs.usda.gov/sites/default/files/2025-01/Field-Book-for-Describing-and-Sampling-Soils-v4.pdf USDA subsample_ID Each soil textural class was sub-sampled 11 times Producer Defined NA Sample type is not soil Producer defined 1 11 replicate_ID Each sub-sample of soil extracted was run with 8 technical replicates Producer Defined NA Sample type is not soil Producer defined 1 8 Well_ID Sample placement on a 96-well plate Producer Defined Each well has an alphanumeric label for rows A-H and columns 1-12 Time_to_threshold Time at which fluorescence amplification for soil samples is distinguishable from the background fluorescence Producer Defined << empty cell >> Sample type is not soil Producer defined A threshold level was calculated for each plate from the wells containing soil samples. This threshold was calculated by adding ten times the standard deviation of the raw fluorescence values from cycles 3-14 to the mean of raw fluorescence values from cycles 3-14. In the MARS software of the Omega plate reader, we input the plate threshold value, and the software calculated the time to threshold for each well. We only report these thresholds for the soil samples. Raw_data_00.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2490 63319 Raw_data_00.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2063 32549 Raw_data_00.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2098 29877 Raw_data_00.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2083 30745 Raw_data_01.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2053 32149 Raw_data_01.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2026 32434 Raw_data_01.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2012 33358 Raw_data_01.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2001 32456 Raw_data_02.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1993 32073 Raw_data_02.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2011 32890 Raw_data_02.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1968 31795 Raw_data_02.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2008 32388 Raw_data_03.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1928 31923 Raw_data_03.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1967 32662 Raw_data_03.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1925 32129 Raw_data_03.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1967 32274 Raw_data_04.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1931 31681 Raw_data_04.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1915 31246 Raw_data_04.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1918 31640 Raw_data_04.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1901 35266 Raw_data_05.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1884 38667 Raw_data_05.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1910 41408 Raw_data_05.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1904 43298 Raw_data_05.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1913 45157 Raw_data_06.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1929 46587 Raw_data_06.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1906 57199 Raw_data_06.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1914 86330 Raw_data_06.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1906 106121 Raw_data_07.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1932 110102 Raw_data_07.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1896 121899 Raw_data_07.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1899 120581 Raw_data_07.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1906 127841 Raw_data_08.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1898 133475 Raw_data_08.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1879 136399 Raw_data_08.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1908 143829 Raw_data_08.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1839 152689 Raw_data_09.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1863 155042 Raw_data_09.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1869 163678 Raw_data_09.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1888 169216 Raw_data_09.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1821 165382 Raw_data_10.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1886 169341 Raw_data_10.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1864 167963 Raw_data_10.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1889 168852 Raw_data_10.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1883 167840 Raw_data_11.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1880 170902 Raw_data_11.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1856 204463 Raw_data_11.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1879 217020 Raw_data_11.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1869 223600 Raw_data_12.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1855 232421 Raw_data_12.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1824 229690 Raw_data_12.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1878 242898 Raw_data_12.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1887 253495 Raw_data_13.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1853 260000 Raw_data_13.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1847 260000 Raw_data_13.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1884 260000 Raw_data_13.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1857 260000 Raw_data_14.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1856 260000 Raw_data_14.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1849 260000 Raw_data_14.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1853 260000 Raw_data_14.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1883 260000 Raw_data_15.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1859 260000 Raw_data_15.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1874 260000 Raw_data_15.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1857 260000 Raw_data_15.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1875 260000 Raw_data_16.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1883 260000 Raw_data_16.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1892 260000 Raw_data_16.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1893 260000 Raw_data_16.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1834 260000 Raw_data_17.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1831 260000 Raw_data_17.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1862 260000 Raw_data_17.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1835 260000 Raw_data_17.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1862 260000 Raw_data_18.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1860 260000 Raw_data_18.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1863 260000 Raw_data_18.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1867 260000 Raw_data_18.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1875 260000 Raw_data_19.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1863 260000 Raw_data_19.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1850 260000 Raw_data_19.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1853 260000 Raw_data_19.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1834 260000 Raw_data_20.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1847 260000 Raw_data_20.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1888 260000 Raw_data_20.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1869 260000 Raw_data_20.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1849 260000 Raw_data_21.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1852 260000 Raw_data_21.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1840 260000 Raw_data_21.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1871 260000 Raw_data_21.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1889 260000 Raw_data_22.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1873 260000 Raw_data_22.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1858 260000 Raw_data_22.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1862 260000 Raw_data_22.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1872 260000 Raw_data_23.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1837 260000 Raw_data_23.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1832 260000 Raw_data_23.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1883 260000 Raw_data_23.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1865 260000 Raw_data_24.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1868 260000 Raw_data_24.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1893 260000 Raw_data_24.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1879 260000 Raw_data_24.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1908 260000 Raw_data_25.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1894 260000 Raw_data_25.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1847 260000 Raw_data_25.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1894 260000 Raw_data_25.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1888 260000 Raw_data_26.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1895 260000 Raw_data_26.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1891 260000 Raw_data_26.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1877 260000 Raw_data_26.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1903 260000 Raw_data_27.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1904 260000 Raw_data_27.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1911 260000 Raw_data_27.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1926 260000 Raw_data_27.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1910 260000 Raw_data_28.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1870 260000 Raw_data_28.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1915 260000 Raw_data_28.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1897 260000 Raw_data_28.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1919 260000 Raw_data_29.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1909 260000 Raw_data_29.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1891 260000 Raw_data_29.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1898 260000 Raw_data_29.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1909 260000 Raw_data_30.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1916 260000 Raw_data_30.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1922 260000 Raw_data_30.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1902 260000 Raw_data_30.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1900 260000 Raw_data_31.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1883 260000 Raw_data_31.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1899 260000 Raw_data_31.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1876 260000 Raw_data_31.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1872 260000 Raw_data_32.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1925 260000 Raw_data_32.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1903 260000 Raw_data_32.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1907 260000 Raw_data_32.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1933 260000 Raw_data_33.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1908 260000 Raw_data_33.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1879 260000 Raw_data_33.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1937 260000 Raw_data_33.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1960 260000 Raw_data_34.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1860 260000 Raw_data_34.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1919 260000 Raw_data_34.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1901 260000 Raw_data_34.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1871 260000 Raw_data_35.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1937 260000 Raw_data_35.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1949 260000 Raw_data_35.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1927 260000 Raw_data_35.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1933 260000 Raw_data_36.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1941 260000 Raw_data_36.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1886 260000 Raw_data_36.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1919 260000 Raw_data_36.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1924 260000 Raw_data_37.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1949 260000 Raw_data_37.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1878 260000 Raw_data_37.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1976 260000 Raw_data_37.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1915 260000 Raw_data_38.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1905 260000 Raw_data_38.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1943 260000 Raw_data_38.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1909 260000 Raw_data_38.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1957 260000 Raw_data_39.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1932 260000 Raw_data_39.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1955 260000 Raw_data_39.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1931 260000 Raw_data_39.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1941 260000 Raw_data_40.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1954 260000 Raw_data_40.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1937 260000 Raw_data_40.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1927 260000 Raw_data_40.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1965 260000 Raw_data_41.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1953 260000 Raw_data_41.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1985 260000 Raw_data_41.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1923 260000 Raw_data_41.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1967 260000 Raw_data_42.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1942 260000 Raw_data_42.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1922 260000 Raw_data_42.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1913 260000 Raw_data_42.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1933 260000 Raw_data_43.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1949 260000 Raw_data_43.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1937 260000 Raw_data_43.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1990 260000 Raw_data_43.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1958 260000 Raw_data_44.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1961 260000 Raw_data_44.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1967 260000 Raw_data_44.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1959 260000 Raw_data_44.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1997 260000 Raw_data_45.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1988 260000 Raw_data_45.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 1994 260000 Raw_data_45.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2032 260000 Raw_data_45.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2011 260000 Raw_data_46.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2021 260000 Raw_data_46.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2078 260000 Raw_data_46.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2025 260000 Raw_data_46.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2014 260000 Raw_data_47.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2037 260000 Raw_data_47.25_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2041 260000 Raw_data_47.50_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2013 260000 Raw_data_47.75_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2018 260000 Raw_data_48.00_hr Fluorescence data recorded at the time defined in the column label Producer Defined 2073 260000 U.S. Geological Survey - ScienceBase mailing address

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Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P13AJ49L None 20260401 Wendy C. Turner USGS Wisconsin Cooperative Wildlife Research Unit mailing and physical

1630 Russell Laboratories

Madison WI 53706 608-263-6882 wturner@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999