Evelyn Pantelopoulos James J. Roberts Matthew R. Acre John Dettmers Matthew D. Faust Corbin D. Hilling Song Qian Christine M. Mayer Branden E. Kohler 20260513 Comma Separated Values (CSV) and R HTML files Reston, VA U.S Geological Survey [(Author Name)(Author Affiliation)(Author ORCID)]: [(Evelyn Pantelopoulos)(University of Southern Mississippi, Department of Coastal Sciences)(https://orcid.org/0009-0003-2884-6972)] [(James J. Roberts)(U.S Geological Survey Great Lakes Science Center, Lake Erie Biological Station)(https://orcid.org/0000-0002-4193-610X)] [(Matthew R. Acre)(U.S Geological Survey Columbia Environmental Research Center)(https://orcid.org/0000-0002-5417-9523)] [(John Dettmers)(Great Lakes Fishery Commission)(https://orcid.org/0000-0003-1061-210X)] [(Matthew D. Faust)(Ohio Department of Natural Resources Division of Wildlife, Sandusky Fisheries Research Station)(https://orcid.org/0009-0006-0928-0872)] [(Corbin D. Hilling)(U.S Geological Survey Great Lakes Science Center, Lake Erie Biological Station)(https://orcid.org/0000-0003-4040-9516)] [(Song Qian)(University of Toledo, Lake Erie Center)(https://orcid.org/0000-0002-2346-4903)] [(Christine M. Mayer)(University of Toledo, Lake Erie Center)(https://orcid.org/0000-0003-4621-5851)] [(Branden Kohler)(Michigan State University, Department of Fisheries and Wildlife)(https://orcid.org/0009-0005-9159-9761)] https://doi.org/10.5066/P1IKFXW2 This data release contains detection and positional data from acoustically tagged fishes used to determine if fish species in the Sandusky River migrate through a location proposed for an invasive species deterrent. Five species—Shorthead Redhorse (Moxostoma macrolepidotum), White Bass (Morone chrysops), Channel Catfish (Ictalurus punctatus), Freshwater Drum (Aplodinotus grunniens), and Smallmouth Buffalo (Ictiobus bubalus)—were tagged to capture various migratory behaviors and life history strategies that may interact with deterrent systems differently. Fish detection and positional data were collected from 2022-2023 and were used to estimate weekly and monthly probabilities of each fish crossing the proposed deterrent site. Grass carp (Ctenopharyngodon idella) detection and positional data from 2022-2023 were also compiled to compare with the native fish movements. The purpose of this research was to develop a better understanding of how native fish species utilize Lake Erie and it's tributaries including the Sandusky River as well as to inform on the operation and implementation of invasive species deterrents. 2022 2023 ground condition Complete None planned Lake Erie and surrounding tributaries -83.6169 -78.7939 42.9403 41.2241 ISO 19115 Topic Category biota Marine Realms Information Bank (MRIB) keywords fisheries biology USGS Thesaurus telemetry fish migration (organisms) population and community ecology User Defined Acoustic Telemetry Deterrent Moxostoma macrolepidotum Shorthead redhorse Morone chrysops White bass Ictalurus punctatus Channel catfish Aplodinotus grunniens Freshwater drum Ictiobus bubalus Smallmouth buffalo Ctenopharyngodon idella Grass carp Tagging USGS Metadata Identifier USGS:698b8608b66b017b73457efc Common geographic areas Ohio Great Lakes Geographic Names Information System (GNIS) Lake Erie Sandusky River Maumee River Integrated Taxonomic Information System (ITIS) Moxostoma macrolepidotum Ictiobus bubalus Ctenopharyngodon idella Ictalurus punctatus Morone chrysops Aplodinotus grunniens Ictiobus bubalus Moxostoma anisurum Amia calva Kingdom Animalia animals Subkingdom Bilateria triploblasts Infrakingdom Deuterostomia Phylum Chordata chordates Subphylum Vertebrata vertebrates Infraphylum Gnathostomata Superclass Actinopterygii ray-finned fishes spiny rayed fishes Class Teleostei Superorder Ostariophysi Order Cypriniformes minnows suckers Superfamily Cobitoidea Family Catostomidae suckers Subfamily Catostominae Tribe Moxostomatini Genus Moxostoma redhorse suckers redhorses Species Moxostoma macrolepidotum Shorthead Redhorse TSN: 163928 Species Moxostoma anisurum Silver Redhorse TSN: 163933 Subfamily Ictiobinae Genus Ictiobus buffalo suckers buffalo fishes Species Ictiobus bubalus Smallmouth Buffalo TSN: 163955 Superfamily Cyprinoidea Family Cyprinidae carps minnows carps and minnows Genus Ctenopharyngodon grass carps Species Ctenopharyngodon idella grass carp silver orfe TSN: 163537 Order Siluriformes catfishes Family Ictaluridae bullhead catfishes North American freshwater catfishes North American catfishes Genus Ictalurus channel catfishes forktail catfishes Species Ictalurus punctatus Channel Catfish graceful catfish TSN: 163998 Superorder Acanthopterygii Order Perciformes perch-like fishes Suborder Percoidei Family Moronidae temperate basses Genus Morone striped basses Species Morone chrysops White Bass White Bass Yellow Bass hybrid White Bass White Perch hybrid Wiper hybrid Whiterock Bass hybrid Sunshine Bass hybrid Palmetto Bass hybrid TSN: 167682 Family Sciaenidae croakers drums roncadores drums and croakers coakers Genus Aplodinotus river drums thunderpumpers freshwater drums Species Aplodinotus grunniens freshwater drum TSN: 169364 Class Holostei holosteans Order Amiiformes bowfin Family Amiidae bowfins bowfin Genus Amia bowfin Species Amia calva Bowfin Ruddy Bowfin TSN: 161104 No access constraints. No use constraints Branden Kohler USGS - Lake Erie Biological Station mailing and physical

380 Huron Street

Huron OH 44839 USA 419-625-1976 Raw data were processed according to manufacturer instructions using factory calibration data to help ensure that retrieved files displayed accurate data. In both predation experiments, acoustic receivers were checked prior to use to ensure information was being logged accurately and firmware was updated. No single logical consistency step was performed. Not applicable. Reported latitude and longitude were collected with a shipboard GPS (WGS-84). All coordinates have been checked for accuracy using Google Earth Pro software. Not applicable. Christopher S. Vandergoot Karen J. Murchie Steven J. Cooke John M. Dettmers Roger A. Bergstedt David G. Fielder 20111128 publication North American Journal of Fisheries Management vol. 31, issue 5 n/a Oxford University Press (OUP) ppg. 914-922 https://doi.org/10.1080/02755947.2011.629717 Digital and/or Hardcopy 20111128 publication date Vandergoot et. al., 2011 The procedure developed for using electroanesthesia and tagging fish with acoustic telemetry transmitters is described in this article. This procedure is a central component of Great Lakes Science Center fish tagging operations and follows IACUC standards. Benjamin Altmann Kurt Hornik Tomas Kalibera Konstanze Lauseker Uwe Ligges Sebastian Meyer Jeroen Ooms Brian D. Ripley Simon Urbanek 2025 4.3.3 application/service https://www.r-project.org/ The R Foundation https://cran.r-project.org/CRAN_team.htm Digital and/or Hardcopy 201603 202604 observed Cran.R-Project CRAN. R-Project is a repository for packages and information related to R and was utilized for modeling the instances of native fish crossing the proposed deterrent site as well as estimating weekly and monthly probabilities of crossing events. Evelyn Pantelopoulos 20260406 application/service https://github.com/epantel/VPS-crossing-rates/tree/main Digital and/or Hardcopy 20260406 publication date VPS_Crossing_Rates This R code was used to estimate the crossing rates of tagged native fishes within the Sandusky River over the location of a hypothetical barrier to limit invasive Grass carp (Ctenopharyngodon idella) spawning in the river. The markdown code provided estimates crossing rates for a variety of tagged fishes within an acoustic telemetry array. The five species analyzed here are: shorthead redhorse, white bass, channel catfish, freshwater drum, and smallmouth buffalo. The acoustic array was located in the Sanduskry River (OH), Lake Erie and other Lake Erie Tributaries. Chris Holbrook Tom Hayden Tom Binder Jon Pyre Alex Nunes 20170301 application/service https://github.com/ocean-tracking-network/glatos Digital and/or Hardcopy 20170301 20251001 observed GLATOS The GLATOS GitHub is a repository for packages and information related to using R to process, analyze, simulate and visualize the acoustic telemetry data collected from the tagged grass carp (Ctenopharyngodon idella) as they moved near different receiver stations. Tagging of native fish: All work and tagging for this work was done as a component of the Lake Erie Native Fish Sandusky River project (LENAT) associated with GLATOS (Great Lakes Acoustic Telemetry Observation System). Tagging events occurred on 7/1/2021, 4/14/2022-4/15/2022, 4/26/2022-4/27/2022, 4/19/2023, 4/26/2023-4/27/2023, and 5/4/2023 on the Sandusky River near the Darr Root Fishing Access in Fremont, Ohio. The fish were collected for utilizing boat electrofishing performed by the Ohio Department of Natural Resources and collected from both above and below the proposed deterrent location. Individual captured native fish had biometric data collected (species, length, weight, sex when possible) before being were anesthetized using transcutaneous electrical nerve stimulation (TENS) unit (MAXTENS 1000; Bio Protech, Chino, California). After immobilization, acoustic transmitters (Innovasea Inc., Halifax, Nova Scotia; transmitter model: V13H, diameter = 13mm, length = 36mm, weight = 11g, 160-260 second nominal delay) have been implanted in the body cavities of fish through ventral incisions, with the incisions then closed using 2 to 3 absorbable monofilament sutures (PDS-II, 3-0, Ethicon, Somerville, NJ). All anesthetization and surgical procedures followed procedures described in Vandergoot et.al, 2011. 177 fish were tagged across five species from 2021-2023 that were analyzed: Channel catfish (n = 15) Freshwater drum (n = 15) Shorthead redhorse (n = 16) Smallmouth buffalo (n = 53) White bass (n = 78) Vandergoot et. al., 2011 GLATOS 2021 Detection of tagged fish: Tagged native fish were detected on acoustic receivers (VR2W, VR2TX, VR2AR, VR4; Innovasea Inc., Nova Scotia, CA). These receivers were arranged in two configurations: (1) receivers in the Sandusky and Maumee rivers were arranged as part of a VEMCO positioning system (VPS) array that allowed for accurate two-dimensional positional estimates when a single transmission event is detected on at least three receivers, and (2) non-positional arrays that cover significantly more area but have a reduced density of receivers providing information when a acoustic transmitter was detected within the range of the fixed receiver. Three of the smallmouth buffalo were not detected during the data collection window but all other fish were. 2023 Quantifying positional data and crossing probabilities: Using the following packages in R (Version 4.3.3, https://cran.r-project.org), positional data for the tagged fish over time was utilized to quantify instances of native fish species crossing the proposed deterrent site as well as estimating weekly and monthly probabilities of crossing events: (glatos v. 0.9.4) (dplyr v. 1.2) (tidyr v. 1.3.2) (lubridate v. 1.9.5) (ggplot2 v. 4.0.2) (sf v. 1.0-24) (purrr v. 1.2.1) (ISOweek v. 0.6-2) (lme4 v. 1.1-38) (arm v. 1.15) (lattice v. 0.22-9) (rv v. 2.3.5) The code and analysis process can be found at https://github.com/epantel/VPS-crossing-rates/tree/main VPS_Crossing_Rates Cran.R-Project 2024 positions_2022_2023.csv Comma Separated Value (CSV) file containing data. The positions_2022_2023.csv file is the combined data set showing the grass carp (Ctenopharyngodon idella) detections within the Sandusky River Vemco Positioning System. This file includes information on fish ID, detection date/time, GPS coordinates of the detection as well as horizontal positioning error. This was used to determine grass carp movement throughout the Sandusky River in reference to the planned deterrent location. Producer Defined FullId Unique value given to each tagged fish used to identify the fish. Producer Defined The full ID is a combination of the code space described in the detection file and the transmitter ID. Time Date and time of when the treatment tags were detected. Time is in 24-hour UTC (Coordinated Universal Time). Producer Defined Format of the date/time stamps was yyyy-mm-dd hh:mm:ss. HPEs The horizontal positional error of the fish detections based on the theoretical accuracy of the receivers in certain environmental conditions. Calculated as a distance but not interpreted as a distance because the detection ranges aren't calibrated. Producer Defined 1.480293605 1251069.996 RMSE Root Mean Square Error. This value is highly variable due to it calculating the amount of error in the estimated horizontal position error of the fish. Producer Defined 0.0 946.2453 Longitude The predicted longitude of the fish detection based on the conditions at the time of detection. Data collected via shipboard GPS unit (WGS-84). Producer Defined -83.12817 -83.01338 Decimal Degrees Latitude The predicted longitude of the fish detection based on the conditions at the time of detection. Data collected via shipboard GPS unit (WGS-84). Producer Defined 41.28741 41.45494 Decimal Degrees. LENAT_detections_2022_2023.csv Comma Separated Value (CSV) file containing data. The LENAT_detections_2022_2023.csv data set includes all detections of tagged native fish made throughout this study. This included information on the tagged fish (species, capture location, length/weight/sex, release location), and information on the receiver that recorded the detection. Producer Defined animal_id Unique value given to each tagged fish used to identify the fish. Producer Defined The alpha-numeric codes were assigned as an based on the transmitter tag implanted in the fish. detection_timestamp_utc Date and time of when the transmitter tags were detected. Time is in 24-hour UTC (Coordinated Universal Time). Producer Defined Format of the date/time stamps was yyyy-mm-dd hh:mm:ss. glatos_array Three letter code indicating the project array this receiver was deployed as part of. A component of the station value. Producer Defined Three letter code indicating the project array this receiver was deployed as part of. These values are selected at the start of GLATOS projects and are subject to change. A list of GLATOS projects can be found on https://glatos.org/projects. station_no Number that is associated with the glatos_array to define a particular site within a GLATOS project. Producer Defined 1 1010 transmitter_codespace Combination of letters and numbers which indicates how a tag transmission is encoded and received. Combining the codespace and the transmitter ID results in a unique transmitter identifier. Producer Defined The transmitter codespace indicates the frequency of the transmitter (A69 = 69 kHz) and the four digit combination afterward is based on several factors including the type of coding in the tag, the number of pings in the transmission and the type of information being transmitted. transmitter_id Unique numerical value that indicates the transmitter being detected. Combining the code space with the transmitter ID results in a unique transmitter ID. Producer Defined 23170 32745 deploy_lat The latitude of the acoustic receiver location. Data collected via shipboard GPS unit (WGS-84). Producer Defined 41.27738 42.73258 deploy_long The longitude of the acoustic receiver. Data collected via shipboard GPS unit (WGS-84). Producer Defined -83.49610 -79.13234 receiver_sn The unique serial number of the acoustic receiver that made the detection. Producer Defined 109125 553171 common_name_e The common names of the tagged fish species that were detected. Corresponds with the scientific names listed in the taxonomy section of the metadata. Producer Defined List of different tagged fish species that were detected throughout the project duration. All common and scientific names were included in the ITIS Taxonomy information. capture_location The approximate location where the fish was captured to be tagged. Deployment Latitude and Deployment Longitude will have more precise capture information. Producer Defined NA No data recorded. Producer defined Sandusky River, Darr Root Ramp The Sandusky River Darr-Root public access boat launch in Fremont, Ohio. Producer defined length The total length of the fish at the time of capture. Producer Defined 0.275 0.838 meters weight The weight of the fish at the time of capture. Producer Defined NA No data currently available. Producer defined The various weights of the tagged fish at the time of capture. sex The sex of the fish identified at the time of capture. Producer Defined NA No data available. Producer defined M Male. Producer defined F Female. Producer defined release_location The location where the fish were released after tagging. Release Latitude and Deployment Longitude will have more precise release information. Producer Defined Sandusky River, Darr Root ramp Darr-Root Public Fishing Access on the Sandusky River in Fremont, Ohio. Producer defined Sandusky River, Tackle Box Ramp Tackle Box Ramp on the Sandusky River located in Fremont, Ohio. Producer defined release_latitude The latitude of the release location. Data collected via shipboard GPS unit (WGS-84). Producer Defined 41.35171 41.35643 Decimal Degrees release_longitude The longitude of the release location. Data collected via shipboard GPS unit (WGS-84). Producer Defined -83.10702 -83.10244 Decimal Degrees utc_release_date_time Date and time of when the tagged fish was released. Time is in 24-hour UTC (Coordinated Universal Time). Producer Defined Format of the date/time stamps was yyyy-mm-dd hh:mm:ss. glatos_project_receiver The GLATOS project of the receiver that made the fish detection. A list of GLATOS projects can be found on https://glatos.org/projects. Producer Defined LEGRC Lake Erie Grass Carp Producer defined LENOP Western Basin Lake Erie Northern Pike. Producer defined SRVPS Sandusky River Vemco Positioning System. Producer defined LEGRD Lake Erie Grid. Producer defined LENSA Lake Erie Nearshore Array. Producer defined LEYPA Lake Erie Yellow Perch Array. Producer defined DRJLS Detroit River Juvenile Lake Sturgeon. Producer defined WAEMF Lake Erie Walleye Mixed Fisheries. Producer defined LEWHF Lake Erie Lake Whitefish. Producer defined HECST Lake Sturgeon Metapopulation Structure. Producer defined LESMB Lake Erie Smallmouth Bass Spatial Ecology. Producer defined EGRWE Lake Erie Grand River Walleye. Producer defined glatos_tag_recovered Indicates if the telemetry tag has been successfully recovered from the fish after harvest or fishing incident. Producer Defined no The acoustic telemetry tag was not recovered as of this data release. Producer defined yes The acoustic telemetry tag was recovered as of this data release. Producer defined glatos_caught_date Date and time of when the treatment tags were recovered/harvested. Time is in 24-hour UTC (Coordinated Universal Time). Producer Defined NA There is no data because the fish was never recovered/harvested. Producer defined 2022-05-01 2022-05-04 station Identifier that combines the GLATOS array and the station number. A list of GLATOS projects can be found on https://glatos.org/projects. Producer Defined Identifier that combines the GLATOS array and the station number. A list of GLATOS projects can be found on https://glatos.org/projects. min_lag The minimum time interval (seconds) between the current detection and another detection from the same tag on the same receiver. Field is used to identify false detections. Producer Defined NA Not detected again on the same receiver. Producer defined 5 61534620 Seconds GLATOS_receiverLocations_2022_2023.csv The GLATOS_receiverLocations_2022_2023 dataset contains all the information regarding the acoustic telemetry receivers in Lake Erie and it's tributaries that made detections during the study timeframe. This includes array information, station codes, deployment and recovery date/time, location data, instrument information, and projects. Producer Defined station Combined GLATOS array and station number. Producer Defined A combination code including both the GLATOS array as well as the station number within the array for site identification. glatos_array Three letter code indicating the project array this receiver was deployed as part of. A component of the station value. A list of GLATOS projects can be found on https://glatos.org/projects. Producer Defined Three letter alphamumeric code indicating the project array this receiver was deployed as part of. A component of the station value. A list of GLATOS projects can be found on https://glatos.org/projects. station_no The site code within a particular GLATOS array. This is a component of Station. Producer Defined 1 1010 consecutive_deploy_no Count of how many times receivers have been deployed at a particular station over the course of the project. Producer Defined 1 24 Deployments deploy_lat Latitude for receiver deployments. Data collected via shipboard GPS unit (WGS-84). Producer Defined 41.27738 42.91848 Decimal Degrees deploy_long Longitude for receiver deployment. Data collected via shipboard GPS unit (WGS-84). Producer Defined -83.61042 -78.8667 Decimal Degrees recover_lat Recovery latitude for the receiver. Data collected via shipboard GPS unit (WGS-84). Producer Defined NA No significant difference between the deployment location and the recovery location. Producer defined 41.3087 42.81001 Decimal Degrees recover_long Recovery longitude for the receiver. Data collected via shipboard GPS unit (WGS-84). Producer Defined NA No significant difference between the deployment location and the recovery location. Producer defined -83.4667 -78.8694 Decimal Degrees deploy_date_time The deployment date and time for the receiver in UTC. Producer Defined Date/Time data is recorded in mm/dd/yyyy hh:mm:ss. recover_date_time The recovery date and time for the receiver in UTC. Producer Defined Date/Time data is recorded in mm/dd/yyyy hh:mm:ss. ins_model_no Type of acoustic receiver deployed. All receivers used in this project were developed by Innovasea. Producer Defined VR2W 69 kHz acoustic receiver developed by Innovasea. Producer defined VR2TX 69 kHz acoustic receiver developed by Innovasea. Producer defined VR2AR 69 kHz acoustic receiver developed by Innovasea. Producer defined VR2C 69 kHz real-time receiver developed by Innovasea. Producer defined VR4 69 and 180 kHz acoustic release receiver developed by Innovasea. Producer defined RXLive 69 kHz real-time receiver developed by Innovasea. Producer defined glatos_ins_frequency Instrument detection frequency band. Producer Defined 69 180 kilohertz (kHz) ins_serial_no Serial number of the receiver. Producer Defined 109123 1132192 code_map The collection of transmitter code spaces that receivers are programed to listen for. This is periodically updated over time. Producer Defined NA No Data Producer defined MAP-115 MAP-115, used in 69 kHz receivers. Producer defined MAP-114 MAP-114, used in 69 kHz receivers. Producer defined MAP-113 MAP-113, used in 69 kHz receivers. Producer defined MAP-115 MAP-414 MAP-115 and MAP-414, a combination of code maps used in VR4 acoustic release receivers. Producer defined Generation-2 Generation-2, the most updated code map for 69 kHz receivers. Producer defined code_map_comment Comments on any issues present with the code map. Producer Defined NA No issues or concerns with the code map for the receiver. Producer defined Free text describing any issues or information about the code-map. comments Additional comments or notes about the receiver either during deployment or after recovery. Producer Defined NA No additional comments or issues. Producer defined Variety of comments about specific instances and issues with a receiver either during deployment or recovery. This includes notes on chain lengths, recovery efforts, attached weights and drag lines. glatos_seasonal Information on if this receiver was being deployed on a seasonal basis for longer-term projects or not. Producer Defined No The receiver will not be maintained and deployed at this location beyond a short-term deployment. Producer defined Yes The receiver will be maintained and deployed at this station multiple times. Producer defined glatos_project Project codes used to identify a GLATOS project. A list of GLATOS projects can be found on https://glatos.org/projects. Producer Defined HECST Lake Sturgeon Metapopulation Structure. Producer defined LENSA Lake Erie Nearshore Array. Producer defined LEGRD Lake Erie Grid. Producer defined EGRWE Lake Erie Grand River Walleye. Producer defined LEWHF Lake Eire Lake Whitefish. Producer defined DRJLS Detroit River Juvenille Lake Sturgeon. Producer defined LEGRC Lake Erie Grass Carp. Producer defined LENOP Western Basin Lake Erie Northern Pike. Producer defined WAEMF Lake Erie Walleye Mixed Fisheries. Producer defined SRVPS Sandusky River Vemco Positioning System. Producer defined LEYPA Lake Erie Yellow Perch Array. Producer defined LESMB Lake Erie Smallmouth Bass Spatial Ecology. Producer defined glatos_vps Information on if this receiver was part of a positional system or part of a non-positional array. Producer Defined No Non-positional array used to gather detection information. Producer defined Yes Vemco positional array used to detect finer-scale 2D movement within a sampling range. Producer defined U.S. Geological Survey - ScienceBase mailing address

Denver Federal Center

Building 810

Mail Stop 302

Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Digital Data https://doi.org/10.5066/P1IKFXW2 None 20260513 Great Lakes Science Center mailing and physical

1451 Green Road

Ann Arbor MI 48105 U.S. 734-994-3331 GS_ASK_GLSC@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999