Melissa M. Baustian Emily J. Lain Sibel Bargu Jacob Cheng Scott Mize Ryder Myers Bingqing Liu Francesca Messina Ioannis Y. Georgiou 20250930 spreadsheet Reston, VA U.S. Geological Survey https://doi.org/10.5066/P13URVEC Monthly surface water samples were collected at sites in the Lake Pontchartrain estuary to assess the phytoplankton biomass and composition using algal pigment analysis. A total of 27 algal pigments including chlorophyll a and accessory pigments were evaluated from each of the water samples. Using algal pigments from surface waters to explore the phytoplankton biomass and composition. Author ORCIDs: Baustian, M.M.(0000-0003-2467-2533);Mize, S.(0000-0001-6751-5568);Myers, R.(0000-0002-1835-9615) 20241120 20250514 ground condition Complete None planned Lake Pontchartrain estuary -90.7031 -89.6292 30.4108 29.9859 ISO 19115 Topic Category biota USGS Thesaurus phytoplankton aquatic ecosystems surface water quality ecology USGS Metadata Identifier USGS:68a4886dd4be023065e4c623 Common geographic areas Louisiana Lake Pontchartrain Lake Maurepas No access constraints. Please see 'Distribution Information' for details. These data are marked with a Creative Commons Zero v1.0 Universal (CC0-1.0) public domain dedication and do not have any use constraints. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Melissa M. Baustian mailing and physical

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Baton Rouge LA 70808 (337) 266-8500 mbaustian@usgs.gov Jay L. Pinckney 20101201 publication Pinckney, J.L. 2010. The USC Method. In The Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4), edited by Stanford B Hooker, Crystal S Thomas, Laurie Van Heukelem, et al. NASA Center for AeroSpace Information. https://ntrs.nasa.gov/citations/20110008482 N/A N/A N/A Water samples were filtered through 25 mm GF/F filters under low vacuum and kept frozen at -80° Celsius in the dark until analyzed for pigments to determine biomass (chlorophyll a) and community composition (accessory pigments) of phytoplankton based on high-performance liquid chromatography methods as described in Pinckney 2010. Pinckney, J.L. 2010. The USC Method. In The Fourth SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-4), edited by Stanford B Hooker, Crystal S Thomas, Laurie Van Heukelem, et al. NASA Center for AeroSpace Information. 20250819 USACE_ERDC_LakeP_HPLC_Phyto_sites.csv Comma Separated Value (CSV) file containing data. Producer Defined SiteLabel 10 site labels from across Lake Pontchartrain estuary, Louisiana, USA Producer Defined Producer defined Producer defined Producer defined C4 Site label Producer defined C7 Site label Producer defined H11 Site label Producer defined HYPER2 Site label Producer defined HYPER3 Site label Producer defined LP10 Site label Producer defined LP11 Site label Producer defined N2 Site label Producer defined PM Site label Producer defined T12 Site label Producer defined Latitude_decimal_degrees Latitude coordinates of site in decimal degrees. Producer Defined 30.07 30.341124 Longitude_decimal_degrees Longitude coordinates of site in decimal degrees. Producer Defined -90.401573 -89.854444 USACE_ERDC_LakeP_HPLC_Phyto_pigments.csv Comma Separated Value (CSV) file containing data. Producer Defined SampleID The unique identifier for the sample that contains the site, replicate (A or B), and the date (MM.DD.YY) it was collected. Producer Defined Unique identifier for the sample. SampleVolume_L Volume (liter) of surface water filtered Producer Defined 0.05 0.2 Chlide_a Chlorophyllide a, microgram per liter Producer Defined 0.0 0.0 Chl_c3 Chlorophyll c3, microgram per liter Producer Defined 0.0 1.08 Chl_c1c2 Chlorophyll c1 + Chlorophyll c2, microgram per liter Producer Defined 0.0 3.36 Peri Peridinin, microgram per liter Producer Defined 0.0 6.81 But-fuco 19'-Butanoyloxyfucoxanthin, microgram per liter Producer Defined 0.0 0.18 Fuco Fucoxanthin, microgram per liter Producer Defined 0.0 2.58 Hex-fuco 19'-Hexanoyloxyfucoxanthin, microgram per liter Producer Defined 0.0 0.0 Neo Neoxanthin, microgram per liter Producer Defined 0.0 0.24 Prasino Prasinoxanthin, microgram per liter Producer Defined 0.0 0.1 Viola Violaxanthin, microgram per liter Producer Defined 0.0 0.22 Diadino Diadinoxanthin, microgram per liter Producer Defined 0.0 2.53 Anth Antheraxanthin, microgram per liter Producer Defined 0.0 0.13 Allo Alloxanthin, microgram per liter Producer Defined 0.0 1.35 Diato Diatoxanthin, microgram per liter Producer Defined 0.0 0.11 Lut Lutein, microgram per liter Producer Defined 0.0 0.49 Zeax Zeaxanthin, microgram per liter Producer Defined 0.0 1.61 Gyro Gyroxanthin-diesther, microgram per liter Producer Defined 0.0 0.0 Chl_b Chlorophyll b, microgram per liter Producer Defined 0.0 1.59 Chl_a Chlorophyll a, microgram per liter Producer Defined 0.58 13.11 a-Car alpha-carotene, microgram per liter Producer Defined 0.0 0.01 b-Car beta-carotene, microgram per liter Producer Defined 0.0 2.23 Myxo Myxoxanthophyll, microgram per liter Producer Defined 0.0 0.0 Croco Crocoxanthin, microgram per liter Producer Defined 0.0 0.06 Echin Echinenone, microgram per liter Producer Defined 0.0 0.23 DVChl_a Divinyl chlorophyll a, microgram per liter Producer Defined 0.0 0.0 Monado Monadoxanthin, microgram per liter Producer Defined 0.0 0.0 Phe_a Pheophytin a, microgram per liter Producer Defined 0.0 0.0 U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P13URVEC None 20250930 Melissa M. Baustian mailing and physical

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Baton Rouge Louisiana 70808 225-508-7796 mbaustian@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999