Bethany K. Kunz Michael G. Iacchetta Danielle M. Cleveland 20250815 tabular digital data Reston, VA U.S. Geological Survey Authors Open Researcher and Contributor Id (ORCID) are as follows: Bethany K. Kunz: 0000-0002-7193-9336; Michael G. Iacchetta: 0000-0001-9459-1435; Danielle M. Cleveland: 0000-0003-3880-4584 https://doi.org/10.5066/P1TJGMCZ The dataset reports biological endpoints (survival, growth, developmental stage, and body burden of selected metals) for larval spadefoot toads (Spea multiplicata) in laboratory-based exposures to 1) field-collected water or sediment from a containment pond at the Pinyon Plain Mine in Arizona, USA, during its pre-production phase and 2) six concentrations of copper or cobalt in water-only bioassays. Also reported are data on water quality and the concentration of metals in the test media during the assays. In the Grand Canyon watershed, native spadefoot toads (Spea multiplicata) may opportunistically use containment ponds associated with breccia pipe uranium mines for egg-laying and aquatic larval development. It is unknown whether chronic exposures to elevated concentrations of metals in the mine ponds can detrimentally affect the survival, growth, and development of larvae or whether metals can be bioaccumulated by S. multiplicata larvae and then transferred into terrestrial food webs when the toads metamorphose. We conducted several types of exposures with S. multiplicata larvae to evaluate these unknowns, including 10-day exposures to field-collected water or sediment from a containment pond at the Pinyon Plain Mine (formerly known as the Canyon Mine) in Arizona, USA, during its pre-production phase. We also conducted 50-day exposures to copper or cobalt in water-only bioassays. These data can be used to better understand potential effects of exposure to mine pond water and sediment for native wildlife. 20210412 20211005 ground condition Complete None planned Pinyon Plain Mine containment pond -112.098076 -112.095898 35.883617 35.881818 ISO 19115 Topic Category biota USGS Thesaurus amphibians bioaccumulation organism growth and development copper cobalt mine waste food web ecotoxicology None metal toxicity trophic transfer USGS Metadata Identifier USGS:689f4888d4be027e7351d922 Common Geographic Areas Coconino Arizona None Columbia Environmental Research Center Pinyon Plain Mine Integrated Taxonomic Information System Spea multiplicata Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Amphibia Order Anura Family Scaphiopodidae Genus Spea Species Spea multiplicata TSN: 206993 None. Please see 'Distribution Info' for details. These data are marked with a Creative Common CC0 1.0 Universal License. These data are in the public domain and do not have any use constraints. It is requested that the authors be cited for any subsequent publications that reference this dataset. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Bethany K Kunz USGS - SOUTHEAST REGION Supervisory Research Ecologist mailing and physical

Columbia Environmental Res Ctr, CERC - Tech Center

Columbia MO 65201 573-441-2998 bkunz@usgs.gov Many thanks to U.S. Geological Survey's Arizona Water Science Center (Flagstaff, AZ), Energy Fuels Inc., and the U.S. Forest Service for their assistance with sample collection; Doug Hardesty, Jo Ellen Hinck, Katy Klymus, Cathy Richter, Jeff Steevens, Nathan Thompson, and Katie Walton-Day (all of USGS) performed the field collections. Thanks to Jo Ellen Hinck for liaising with Energy Fuels to coordinate sampling. Christina Mackey, Holly Puglis, Erinn Scott, and Samantha Tidwell (all of USGS) played important roles in data collection during bioassays. Windows 11 version 23H2, build 22631.3880; notepad++ 64-bit x64 version 8.6.9 Instruments measuring water quality parameters were calibrated regularly using standard solutions. Instruments used in ICP-MS analysis are regularly calibrated by third-party professionals. Data were entered and spot-checked independently by multiple scientists to confirm accurate recording of each observation. All measured data fall within expected ranges. Measured concentrations of metals in the water and sediment fall within ranges expected to be seen in a site associated with breccia pipe mining. Measured concentrations of cobalt and copper in the test water were reasonable, given nominal treatment concentrations. Data were entered into spreadsheets by one scientist and checked for transcription errors by another. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted. No formal positional accuracy tests were conducted. ASTM 2014 publication Annual Book of ASTM International Standards v. 11.06 doi,org/10.1520/E0729-96R14 Digital and/or Hardcopy 2014 publication date ASTM (2014) Standard used to guide toxicity testing methods US Environmental Ptrotection Agency 2002 4th ed publication https://www.epa.gov/sites/default/files/2015-08/documents/short-term-chronic-freshwater-wet-manual_2002.pdf. Digital and/or Hardcopy 2002 publication date EPA (2002) Standard used to guide toxicity testing methods Kenneth L. Gosner 1960 publication Herpetologica Vol. 16, No. 3 https://www.jstor.org/stable/3890061 Digital and/or Hardcopy 19600923 publication date Gosner 1960 reference for staging of tadpoles Daniel R. Buchholz Tyrone B. Hayes 2000 publication Herpetologica Vol. 56, No. 4 https://www.jstor.org/stable/3893113 Digital and/or Hardcopy 2000 publication date Buchholz and Hayes 2000 Methods for rearing tadpoles ASTM 2013 publication Annual Book of ASTM International Standards v. 11.06 doi.org/10.1520/E2591-07R13 Digital and/or Hardcopy 2013 publication date ASTM 2013 Standard used to guide toxicity testing methods Experiment 1. Pinyon Plain Mine pond water exposures:Newly fertilized S. multiplicata eggs were obtained from captive-bred adults maintained in a colony at the University of North Carolina. Eggs were shipped overnight to the USGS Columbia Environmental Research Center (CERC) in dechlorinated tap water at 18 degrees Celsius. After arrival, eggs were slowly transitioned to test temperature (28 degrees Celsius) and acclimated to test water (CERC well water; 280-300 mg/L hardness) over the course of two days. Test endpoints were survival, growth, development, and body burden of metals. The test was conducted based on ASTM (2014) and EPA (2002). Test waters were collected in June 2017 and September 2017 from four areas within the Pinyon Plain Mine containment pond and shipped to CERC. After arrival, waters from all locations on a given sampling date were homogenized and frozen at -20 degrees Celsius. Two days prior to the amphibian test, waters were thawed. The day before the test (Test Day -1 or 20180408), each water sample was mixed, and 800 mL were added to each of three (June 2017) or four (September 2017) replicate chambers (1-L beakers). Three rather than four replicate chambers were used for the June 2017 sample, due to a limited volume of water from that sampling date. CERC well water (800 mL) was added to four replicate chambers as a control. All chambers were placed in a water bath at 28 degrees Celsius. At the beginning of the test (20180409), less than 72-hour-old S. multiplicata tadpoles were assigned impartially to test chambers by placing five organisms in each replicate of either Pinyon Plain Mine June 2017 water, Pinyon Plain Mine September 2017 water, or CERC well water control. Water in each chamber was partially renewed (50%) on Test Day 3 (20180412), 6 (20180415), and 8 (20180417). Chambers were checked daily, with tadpoles recorded as alive or dead (death/immobility defined as lack of movement in response to gentle prodding with a glass rod). After tadpoles reach the feeding stage (Stage 25; Gosner 1960), each chamber of S. multiplicata was fed finely ground rabbit chow daily, with increasing amounts to supplement as needed (methods after Buchholz and Hayes 2000; ASTM 2013). Exposures were conducted for 9 days. At the end of the experiment (20180418), surviving tadpoles were euthanized with MS-222, blotted dry, and measured for snout-vent length, mass, and developmental stage (Gosner 1960). Tadpoles were pooled into three samples per treatment to obtain adequate sample mass and were frozen in precleaned plastic bottles for analysis of whole-body metals. The test acceptability criterion was at least 80% survival in the control treatment. A sample of each water was collected for metals analysis at the beginning of the study (20180409). Major ions and dissolved organic carbon were measured in each treatment water at the beginning (20180409) and end (20180418) of the study, and in control water at the beginning of the study (20180409). Hardness, alkalinity, conductivity, ammonia, and pH were measured in each water and the control water at the beginning (20180409) and end (20180418) of the study. Dissolved oxygen and temperature were determined daily. Tadpole tissues were collected at the end of the study for metals analysis (20180418). 20180418 Experiment 2. Pinyon Plain Mine pond sediment exposure: Newly fertilized S. multiplicata eggs were obtained and acclimated as described for Experiment 1, above. Test endpoints were survival, growth, development, and body burden of metals. The test was conducted based on ASTM E2591 (2013). Test sediments were collected in June 2017 from four areas within the Pinyon Plain Mine containment pond and shipped to CERC. After arrival, sediments from all locations were homogenized and refrigerated at 4 degrees Celsius. The day before the amphibian test (Test Day -1 or 20180501), sediments were rehomogenized and added to eight replicate chambers of each of two types—1-L beakers and 300-mL beakers with a screened overflow. Each 1-L beaker received 200 mL of sediment, and each 300-mL beaker received 100 mL of sediment. Overlying water (CERC well water) was added with minimal sediment disturbance and chambers were left undisturbed overnight. On Test Day 0 (20180502), all beakers were placed in a modified Mount and Brungs diluter at 28 degrees Celsius. With each cycle of the diluter, splitters delivered approximately 125 mL or 250 mL of well water to each replicate 300-mL or 1-L beaker, respectively. At the beginning of the test (20180502), less than 72-hour-old S. multiplicata tadpoles were assigned impartially to test chambers by placing five organisms in one of eight replicates of either Pinyon Plain Mine sediment or a control sediment (Spring River) in 1-L beakers, and three organisms in one of eight replicates of the same treatments in 300-mL beakers. Chambers were checked daily, with tadpoles recorded as alive or dead (death/immobility defined as lack of movement in response to gentle prodding with a glass rod). After tadpoles reach the feeding stage (Stage 25; Gosner 1960), each chamber of S. multiplicata was fed finely ground rabbit chow daily, with increasing amounts to supplement as needed (methods after Buchholz and Hayes 2000; ASTM 2013). Exposures were conducted for 10 days. At the end of the experiment (20180512), surviving tadpoles were euthanized with MS-222, blotted dry, and measured for snout-vent length, mass, and developmental stage (Gosner 1960). Tadpoles were pooled into three or four samples per treatment to obtain adequate sample mass and were frozen in precleaned plastic bottles for analysis of whole-body metals. The test acceptability criterion was at least 80% survival in the control sediment treatment. One sediment sample was collected for metals analysis at the beginning of the study (20180502). Major ions and dissolved organic carbon were measured in well water at the beginning of the study (20180502). Hardness, alkalinity, conductivity, ammonia, and pH were measured in overlying water of the mine sediment and control sediment beakers at the beginning and end of the study. Dissolved oxygen and temperature were measured daily. Tadpole tissues were collected at the end of the study (20180512) for metals analysis. 20180512 Experiments 3 and 4. Chronic toxicity of cobalt and copper: Newly fertilized S. multiplicata eggs were obtained and acclimated as described for Experiment 1, above. Test endpoints for tadpoles remaining at the end of the exposures were survival, growth, developmental stage, and body burden of metals. Test endpoints for individuals that metamorphosed during the exposures were survival, mass at metamorphosis, time to metamorphosis, and body burden of metals. Although no standardized method exists for chronic amphibian tests, test methods were based on standard methods, such as ASTM (2014) and EPA (2002), and on previous chronic amphibian studies at CERC. At the beginning of the test (20180502), less than 72-hour-old S. multiplicata tadpoles were assigned impartially to test chambers by placing five organisms in one of four replicates. Test chambers were placed in a modified Mount and Brungs diluter at 28 degrees Celsius. With each cycle of the diluter, four-way splitters delivered approximately 250 mL of test solution to each replicate at each of five treatment concentrations (Cobalt: 30, 60, 120, 240, and 480 micrograms per liter; Copper: 3.75, 7.5, 15, 30, and 60 micrograms per liter) and a well water control. Chambers were checked daily, with tadpoles recorded as alive or dead (death/immobility defined as lack of movement in response to gentle prodding with a glass rod). After tadpoles reached the feeding stage (Stage 25; Gosner 1960), each chamber of S. multiplicata was fed finely ground rabbit chow daily, with increasing amounts to supplement as needed (methods after Buchholz and Hayes 2000; ASTM 2013). As tadpoles approached forelimb emergence (Stage 42; Gosner 1960), chambers were checked twice per day. At forelimb emergence, each metamorph was removed from exposure in the diluter and placed in a clean, individual plastic container until tail resorption was complete. Exposures were ended on Day 50 (20180621) for any remaining tadpoles. The test acceptability criterion was at least 80% survival in the control treatment. Water samples for metal analysis were collected at each concentration for each metal on Day -1 (20180501), Day 14 (20180516), Day 28 (20180530), and Day 50 (20180621) of each study. Major ions and dissolved organic carbon were measured in the control well water at the beginning of each study (20180501). Hardness, alkalinity, conductivity, ammonia, and pH were measured in the control, low, medium, and high concentration weekly for each study. Dissolved oxygen and temperature were measured daily. Metamorphs were collected for metals analysis once tail resorption was complete and remaining tadpoles were collected for metals analysis on Day 50 (20180621). In some cases, animals were analyzed individually and in other cases individuals were pooled to reach a sufficient mass for analysis. Water samples were preserved with 1% nitric acid before inductively coupled plasma-mass spectrometry (ICP-MS) analysis. ICP-MS analysis was conducted using a PerkinElmer NexION 2000. Sediment and tissue samples were lyophilized and acid-digested using a MARS6 Xpress microwave before ICP-MS analysis was conducted using the same instrument as that utilized for the water samples. 20180621 Water_Sediment_CationsAnionsDOC.csv Comma Separated Value (CSV) file containing the concentration of major cations, anions, and dissolved organic carbon in the test water of the field-collected pond water and sediment assays. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which samples were collected. Producer defined 20180409 20180501 YYYYMMDD CERC_ID A unique five digit integer used to identify an individual sample during ICP-MS analysis. Producer defined A five digit integer. TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined CERC Well water from the Columbia Environmental Research Center (CERC) in Columbia, MO, USA. This well water served as a control treatment in the field-collected water study and as overlying water in the field-collected sediment study. Water hardness is approximately 300 mg/L of CaCO3. Producer defined CM-JW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in June of 2017. Producer defined CM-SW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in September 2017. Producer defined LOCATION The location the exposure SUBSTRATE originated from. Producer defined CERC The Columbia Environmental Research Center located in Columbia, MO, USA. Producer defined Pinyon Plain Mine The Pinyon Plain Mine (formerly known as the Canyon Mine) located 6 miles southeast of Tusayan, AZ, USA. Producer defined SUBSTRATE The type field-collected substrate used as an exposure medium for the corresponding observation. Producer defined Well water Well water supplied in the laboratory where the assays were conducted. Producer defined Pond water Field-collected water collected from within the pond at the corresponding treatment LOCATION. Producer defined FIELD_DATE A literal value defining the month in the year 2017 when the field-collected SUBSTRATE was collected for the experiment. Producer defined NA Not applicable because the substrate was supplied by the laboratory and not field-collected. Producer defined The literal name of the month. MATRIX The type of sample analyzed using ICP-MS. Producer defined Water A water sample was the analyzed matrix Producer defined BID The block initiation date expressed in YYYYMMDD. A representation of time in which the smallest unit of measure is a day. This is a unique date assigned to a group or batch of samples for analysis or other processing to enable QA/QC traceability. Producer defined 20180419 20180523 YYYYMMDD ANALYTE The chemical constituent whose concentration was quantified in the corresponding observation. Producer defined Sodium The 11th element on the periodic table. CAS number 7440-23-5. Producer defined Magnesium The 12th element on the periodic table. CAS number 7439-95-4. Producer defined Potassium The 19th element on the periodic table. CAS number 7440-09-7. Producer defined Calcium The 20th element on the periodic table. CAS number 7440-70-2. Producer defined Manganese The 25th element on the periodic table. CAS number 7439-96-5. Producer defined Iron The 26th element on the periodic table. CAS number 7439-89-6. Producer defined Strontium The 38th element on the periodic table. CAS number 7440-24-6. Producer defined Fluorine The 9th element on the periodic table. CAS number 7782-41-4. Producer defined Chlorine The 17th element on the periodic table. CAS number 7782-50-5. Producer defined Bromine The 35th element on the periodic table. CAS number 7726-95-6. Producer defined Nitrogen as nitrate and nitrite The sum of the 7th element, nitrogen, in nitrate (NO3-) and nitrite (NO2-). Producer defined Phosphate The anion PO4-3. CAS number 14265-44-2. Producer defined Sulfate The anion SO4-2. CAS number 14808-79-8. Producer defined Dissolved organic carbon Organic compounds in the form of carbon that passed through a .45 millimeter filter. Producer defined REPORT_LIMIT A numeric value for the reporting limit of the corresponding ANALYTE. Producer defined 0.01 150.0 See 'Units' attribute VALUE The concentration of the ANALYTE measured in the in the corresponding sample. Producer defined LTRL The measured value was less than the reporting limit. Producer defined 0.1 1400.0 See 'Units' attribute UNITS The standard unit of measure associated with the corresponding VALUE and REPORT_LIMIT. Producer defined A literal value representing the standard unit of measure. Water_Sediment_MediaMetalConcentrations.csv Comma Separated Value (CSV) file containing the concentration of 16 metals measured using ICP-MS in the field-collected pond water and field-collected pond sediment. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which samples were collected. Producer defined 20180409 20180502 YYYYMMDD CERC_ID A unique five digit integer used to identify an individual sample during ICP-MS analysis. Producer defined A five digit integer. TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined CERC The treatment consisted of well water from the Columbia Environmental Research Center (CERC) in Columbia, MO, USA. This well water served as a control treatment. Water hardness is ~300 mg/L of CaCO3. Producer defined CM-JW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in June of 2017. Producer defined CM-SW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in September 2017. Producer defined CM-L/SB The treatment consisted of field-collected pond sediment from the Pinyon Plain Mine site in June of 2017. Producer defined LOCATION The location the exposure SUBSTRATE originated from. Producer defined CERC The Columbia Environmental Research Center located in Columbia, MO, USA. Producer defined Pinyon Plain Mine The Pinyon Plain Mine (formerly known as the Canyon Mine) located 6 miles southeast of Tusayan, AZ, USA. Producer defined SUBSTRATE The type field-collected substrate used as an exposure medium for the corresponding observation. Producer defined Well water Well water supplied in the laboratory where the assays were conducted. Producer defined Pond water Field-collected water collected from within the pond at the corresponding treatment LOCATION. Producer defined Sediment Field-collected substrate collected from the corresponding treatment LOCATION. Producer defined FIELD_DATE A literal value defining the month in the year 2017 when the field-collected SUBSTRATE was collected for the experiment. Producer defined NA Not applicable. The medium was supplied by the laboratory and not field-collected. Producer defined The literal name of the month. MATRIX The type of sample analyzed using ICP-MS. Producer defined Water Treatment water from the experiment. Producer defined Sediment Treatment sediment from the experiment. Producer defined BID The block initiation date expressed in YYYYMMDD. A representation of time in which the smallest unit of measure is a day. This is a unique date assigned to a group or batch of samples for analysis or other processing to enable QA/QC traceability. Producer defined 20180511 20181108 YYYYMMDD ANALYTE The chemical constituent whose concentration was quantified in the corresponding observation. Producer defined Aluminum The 13th element on the periodic table. CAS number 7429-90-5. Producer defined Iron The 26th element on the periodic table. CAS number 7439-89-6. Producer defined Cobalt The 27th element on the periodic table. CAS number 7440-48-4. Producer defined Nickel The 28th element on the periodic table. CAS number 7440-02-0. Producer defined Copper The 29th element on the periodic table. CAS number 7440-50-8. Producer defined Zinc The 30th element on the periodic table. CAS number 7440-66-6. Producer defined Arsenic The 23rd element on the periodic table. CAS number 7440-38-2. Producer defined Selenium The 34th element on the periodic table. CAS number 7782-49-2. Producer defined Molybdenum The 42nd element on the periodic table. CAS number 7439-98-7. Producer defined Silver The 47th element on the periodic table. CAS number 7440-22-4. Producer defined Cadmium The 48th element on the periodic table. CAS number 7440-43-9. Producer defined Thallium The 81st element on the periodic table. CAS number 7440-28-0. Producer defined Lead The 82nd element on the periodic table. CAS number 7439-92-1. Producer defined Bismuth The 83rd element on the periodic table. CAS number 7440-69-9. Producer defined Thorium The 90th element on the periodic table. CAS number 7440-29-1. Producer defined Uranium The 92nd element on the periodic table. CAS number 7440-61-1. Producer defined LOQ A numeric value for the limit of quantification of the corresponding ANALYTE. The limit of quantification may increase if sample dilution is required due to column overload and peak tailing or fronting. Producer defined 0.003 4.0 See 'Units' attribute VALUE The concentration of the ANALYTE measured in the in the corresponding sample. Producer defined LTLOQ The measured value was less than the limit of quantification. Producer defined 0.13 14000.0 See 'Units' attribute UNITS The standard unit of measure associated with the corresponding VALUE and LOQ. Producer defined A literal value representing the standard unit of measure. COMMENTS A description of other important information associated with the corresponding observation. Producer defined << empty cell >> No Data Producer defined A description of other important information associated with the corresponding observation. Water_Sediment_Survival.csv Comma Separated Value (CSV) file containing the number of tadpoles that survived in each replicate of the field-collected pond water and sediment assays. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which samples were collected. Producer defined 20180418 20180512 YYYYMMDD TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined SR-L The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined SR-SB The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 300 mL of field-collected sediment. Producer defined CM-L The treatment consisted of pond sediment collected from the pond at Pinyon Plain Mine. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined CM-SB The treatment consisted of exposure to field-collected pond sediment from the Pinyon Plain Mine. The exposure chamber contained 300 mL of field-collected sediment. Producer defined CERC The treatment consisted of well water from the Columbia Environmental Research Center (CERC) in Columbia, MO, USA. This well water served as a control treatment. Water hardness is approximately 300 mg/L of CaCO3. Producer defined CM-JW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in June of 2017. Producer defined CM-SW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in September 2017. Producer defined LOCATION The location the exposure SUBSTRATE originated from. Producer defined Spring River A site with uncontaminated sediment in the Spring River used as a control treatment for the sediment exposure study. Location on the river is approximately 2 miles south of Waco, Missouri, USA. Coordinates of sediment collection site are N 37.222162, W 94.598575. Producer defined Pinyon Plain Mine The Pinyon Plain Mine (formerly known as the Canyon Mine) located 6 miles southeast of Tusayan, AZ, USA. Producer defined CERC The Columbia Environmental Research Center located in Columbia, MO, USA. Producer defined SUBSTRATE The type field-collected substrate used as an exposure medium for the corresponding observation. Producer defined Sediment Field-collected substrate collected from the corresponding treatment LOCATION. Producer defined Well water Well water supplied in the laboratory where the assays were conducted. Producer defined Pond water Field-collected water collected from within the pond at the corresponding treatment LOCATION. Producer defined FIELD_DATE A literal value defining the month in the year 2017 when the field-collected SUBSTRATE was collected for the experiment. Producer defined NA Not applicable. The medium was supplied by the laboratory and not field-collected. Producer defined The literal name of the month. EXP_CHAMBER The type of experimental chamber used in the corresponding treatment. Producer defined L A 1 liter beaker. Producer defined SB A 300 milliliter sediment beaker. Producer defined REPLICATE A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. Producer defined 1 8 STOCKED The number of tadpoles introduced to the experimental unit at the initiation of the experiment. Producer defined 5 5 count OVR_SURV The total number of tadpoles from the number STOCKED that survived the exposure treatment in the corresponding REPLICATE. Producer defined 1 5 count Water_Sediment_TadpoleBodyBurden.csv Comma Separated Value (CSV) file containing the concentrations of 16 metals measured using ICP-MS in whole body tissue samples of tadpoles after exposure to field-collected pond water and sediment. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which samples were collected. Producer defined 20180418 20180823 YYYYMMDD CERC_ID A unique five digit integer used to identify an individual sample during ICP-MS analysis. Producer defined A five digit integer. TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined CM-SW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in September 2017. Producer defined CM-JW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in June of 2017. Producer defined CERC Well water from the Columbia Environmental Research Center (CERC) in Columbia, MO, USA. This well water served as a control treatment in the field-collected water study and as overlying water in the field-collected sediment study. Water hardness is approximately 300 mg/L of CaCO3. Producer defined CM-L The treatment consisted of pond sediment collected from the pond at Pinyon Plain Mine. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined SR-L The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined CM-SB The treatment consisted of exposure to field-collected pond sediment from the Pinyon Plain Mine. The exposure chamber contained 300 mL of field-collected sediment. Producer defined SR-SB The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 300 mL of field-collected sediment. Producer defined LOCATION The location the exposure SUBSTRATE originated from. Producer defined Pinyon Plain Mine The Pinyon Plain Mine (formerly known as the Canyon Mine) located 6 miles southeast of Tusayan, AZ, USA. Producer defined CERC The Columbia Environmental Research Center located in Columbia, MO, USA. Producer defined Spring River A site with uncontaminated sediment in the Spring River used as a control treatment for the sediment exposure study. Location on the river is approximately 2 miles south of Waco, Missouri, USA. Coordinates of sediment collection site are N 37.222162, W 94.598575. Producer defined SUBSTRATE The type field-collected substrate used as an exposure medium for the corresponding observation. Producer defined Pond water Field-collected water collected from within the pond at the corresponding treatment LOCATION. Producer defined Well water Well water supplied in the laboratory where the assays were conducted. Producer defined Sediment Field-collected substrate collected from the corresponding treatment LOCATION. Producer defined FIELD_DATE A literal value defining the month in the year 2017 when the field-collected SUBSTRATE was collected for the experiment. Producer defined The literal name of the month. EXP_CHAMBER The type of experimental chamber used in the corresponding treatment. Producer defined L A 1 liter beaker. Producer defined SB A 300 milliliter sediment beaker. Producer defined REPLICATE A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. Producer defined A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. MATRIX The type of sample analyzed using ICP-MS. Producer defined Tadpole tissue Pooled whole-body samples of tadpoles. Tadpoles were pooled to reach a sufficient mass for metals analysis. Producer defined BID The block initiation date expressed in YYYYMMDD. A representation of time in which the smallest unit of measure is a day. This is a unique date assigned to a group or batch of samples for analysis or other processing to enable QA/QC traceability. Producer defined 20180914 20180914 YYYYMMDD ANALYTE The chemical constituent whose concentration was quantified in the corresponding observation. Producer defined Aluminum The 13th element on the periodic table. CAS number 7429-90-5. Producer defined Iron The 26th element on the periodic table. CAS number 7439-89-6. Producer defined Cobalt The 27th element on the periodic table. CAS number 7440-48-4. Producer defined Nickel The 28th element on the periodic table. CAS number 7440-02-0. Producer defined Copper The 29th element on the periodic table. CAS number 7440-50-8. Producer defined Zinc The 30th element on the periodic table. CAS number 7440-66-6. Producer defined Arsenic The 23rd element on the periodic table. CAS number 7440-38-2. Producer defined Selenium The 34th element on the periodic table. CAS number 7782-49-2. Producer defined Molybdenum The 42nd element on the periodic table. CAS number 7439-98-7. Producer defined Silver The 47th element on the periodic table. CAS number 7440-22-4. Producer defined Cadmium The 48th element on the periodic table. CAS number 7440-43-9. Producer defined Thallium The 81st element on the periodic table. CAS number 7440-28-0. Producer defined Lead The 82nd element on the periodic table. CAS number 7439-92-1. Producer defined Bismuth The 83rd element on the periodic table. CAS number 7440-69-9. Producer defined Thorium The 90th element on the periodic table. CAS number 7440-29-1. Producer defined Uranium The 92nd element on the periodic table. CAS number 7440-61-1. Producer defined LOQ A numeric value for the limit of quantification of the corresponding ANALYTE. The limit of quantification may increase if sample dilution is required due to column overload and peak tailing or fronting. Producer defined 0.02 40.0 See 'Units' attribute VALUE The concentration of the ANALYTE in the corresponding observation. Producer defined LTLOQ The measured value was less than the limit of quantification. Producer defined 0.06 36000.0 See 'Units' attribute UNITS The standard unit of measure associated with the corresponding VALUE and LOQ. Producer defined The standard unit of measure associated with the corresponding VALUE and LOQ. COMMENTS A description of other important information associated with the corresponding observation. Producer defined A description of other important information associated with the corresponding observation. Water_Sediment_TadpolesGD.csv Comma Separated Value (CSV) file containing the mass, snout-vent length, and Gosner stage of tadpoles at the end of the exposure period in the field-collected pond water and sediment assays. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which samples were collected. Producer defined 20180418 20180512 YYYYMMDD TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined CM-SB The treatment consisted of exposure to field-collected pond sediment from the Pinyon Plain Mine. The exposure chamber contained 300 mL of field-collected sediment. Producer defined SR-SB The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 300 mL of field-collected sediment. Producer defined CM-L The treatment consisted of pond sediment collected from the pond at Pinyon Plain Mine. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined SR-L The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined CERC Well water from the Columbia Environmental Research Center (CERC) in Columbia, MO, USA. This well water served as a control treatment in the field-collected water study and as overlying water in the field-collected sediment study. Water hardness is approximately 300 mg/L of CaCO3. Producer defined CM-JW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in June of 2017. Producer defined CM-SW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in September 2017. Producer defined IND_ID A unique identifier representing an individual tadpole within a replicate for the corresponding observation. Producer defined An alphanumeric identifier, in the format XXXX## where XXXX is the treatment ID and ## are the replicate and individual number within that replicate. LOCATION The location the exposure SUBSTRATE originated from. Producer defined Pinyon Plain Mine The Pinyon Plain Mine (formerly known as the Canyon Mine) located 6 miles southeast of Tusayan, AZ, USA. Producer defined Spring River A site with uncontaminated sediment in the Spring River used as a control treatment for the sediment exposure study. Location on the river is approximately 2 miles south of Waco, Missouri, USA. Coordinates of sediment collection site are N 37.222162, W 94.598575. Producer defined CERC The Columbia Environmental Research Center located in Columbia, MO, USA. Producer defined SUBSTRATE The type field-collected substrate used as an exposure medium for the corresponding observation. Producer defined Sediment Field-collected substrate collected from the corresponding treatment LOCATION. Producer defined Well water Well water supplied in the laboratory where the assays were conducted. Producer defined Pond water Field-collected water collected from within the pond at the corresponding treatment LOCATION. Producer defined FIELD_DATE A literal value defining the month in the year 2017 when the field-collected SUBSTRATE was collected for the experiment. Producer defined NA Not applicable. The medium was supplied by the laboratory and not field-collected. Producer defined The literal name of the month. EXP_CHAMBER The type of experimental chamber used in the corresponding treatment. Producer defined SB A 300 milliliter sediment beaker. Producer defined L A 1 liter beaker. Producer defined REPLICATE A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. Producer defined A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. INDIVIDUAL A numerical identifier of the individual tadpole within the corresponding REPLICATE. Producer defined A numerical identifier of the individual tadpole within the corresponding REPLICATE. PARAMETER A literal value representing the parameter measured. Producer defined Mass The wet weight mass of the tadpole. Producer defined Snout-vent length The straight length measurement from the tip of the snout to the end of the cloaca. Producer defined Gosner stage The developmental stage of the tadpole at the time of the observation. Developmental stage was classified following Gosner (1960). Producer defined VALUE The analytical result for the parameter measured Producer defined 4.85 510.8 See 'Units' attribute UNITS The standard unit of measure for the VALUE Producer defined The standard unit of measure for the VALUE CERC_ID A unique five digit integer used to identify an individual sample during ICP-MS analysis. The is a key attribute linking data between tables. Producer defined NA No Data Producer defined A five digit integer COMMENTS A description of other important information associated with the corresponding observation. Producer defined A description of other important information associated with the corresponding observation. Water_Sediment_WQ.csv Comma Separated Value (CSV) file containing the dissolved oxygen, temperature, specific conductance, alkalinity, pH, hardness, and ammonia concentration of test water in the field-collected pond water and sediment assays. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the samples were collected. Producer defined 20180409 20180511 YYYYMMDD TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined SR-L The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined SR-SB The treatment consisted of pond sediment collected from the pond at Spring River. The exposure chamber contained 300 mL of field-collected sediment. Producer defined CM-L The treatment consisted of pond sediment collected from the pond at Pinyon Plain Mine. The exposure chamber contained 1000 mL of field-collected sediment. Producer defined CM-SB The treatment consisted of exposure to field-collected pond sediment from the Pinyon Plain Mine. The exposure chamber contained 300 mL of field-collected sediment. Producer defined CERC Well water from the Columbia Environmental Research Center (CERC) in Columbia, MO, USA. This well water served as a control treatment in the field-collected water study and as overlying water in the field-collected sediment study. Water hardness is approximately 300 mg/L of CaCO3. Producer defined CM-JW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in June of 2017. Producer defined CM-SW The treatment consisted of field-collected pond water from the Pinyon Plain Mine site in September 2017. Producer defined LOCATION The location the exposure SUBSTRATE originated from. Producer defined Spring River A site with uncontaminated sediment in the Spring River used as a control treatment for the sediment exposure study. Location on the river is about 2 miles south of Waco, Missouri, USA. Coordinates of sediment collection site are N 37.222162, W 94.598575. Producer defined Pinyon Plain Mine The Pinyon Plain Mine (formerly known as the Canyon Mine) located 6 miles southeast of Tusayan, AZ, USA. Producer defined CERC The Columbia Environmental Research Center located in Columbia, MO, USA. Producer defined SUBSTRATE The field-collected substrate used as an exposure medium for the corresponding observation. Producer defined Sediment Field-collected substrate collected from the corresponding treatment LOCATION. Producer defined Water Water from the corresponding treatment LOCATION. Producer defined FIELD_DATE A literal value defining the month in the year 2017 when the field-collected SUBSTRATE was collected for the experiment. Producer defined NA Not applicable. The medium was supplied by the laboratory and not field-collected. Producer defined The literal name of the month. EXP_CHAMBER The type of experimental chamber used in the corresponding treatment. Producer defined L A 1 liter beaker. Producer defined SB A 300 milliliter sediment beaker. Producer defined REPLICATE An identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. The value C-1 represents a composite sample of test water from multiple experimental units of the same treatment. The value C-2 represents a duplicate composite sample of test water. Producer defined A number representing the experimental replicate. The value C-1 represents a composite sample of test water from multiple experimental units of the same treatment. The value C-2 represents a duplicate composite sample of test water. PARAMETER A literal value representing the parameter measured. Producer defined Dissolved oxygen Concentration of gaseous oxygen in water or water solutions, unfiltered, measured as milligrams per liter. Producer defined Temperature Measure of the thermal energy present in water, reported in degrees Celsius. Producer defined Ammonia Concentration of ammonia in the sample, reported as milligrams per liter as total nitrogen. Producer defined Specific Conductance Reciprocal of the resistance in ohms measured between opposite faces of a centimeter cube of an aqueous solution at a specified temperature, 25 degrees Celsius, microsiemens per centimeter. Producer defined pH Logarithm of the reciprocal hydrogen ion concentration in atoms per liter or acidity of water in test chambers. Producer defined Alkalinity The total titratable bases in water or buffering capacity of culture and test waters as equivalent milligrams of calcium carbonate per liter. Producer defined Hardness Alkaline salts in water, mainly calcium and magnesium, as milligrams calcium carbonate per liter. Producer defined VALUE The analytical result of the parameter measured. Producer defined 0.029 2420.0 See 'UNITS' attribute UNITS The standard unit of measure associated with the corresponding VALUE. Producer defined The standard unit of measure associated with the corresponding VALUE. Co_Cu_AllBodyBurdens.csv Comma Separated Value (CSV) file containing the concentration of cobalt or copper measured using ICP-MS in whole body tissue samples of tadpoles and metamorphs in the chronic exposure assays of cobalt and copper. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which samples were collected. Producer defined 20180823 20180823 YYYYMMDD CERC_ID A unique five digit integer used to identify an individual sample during ICP-MS analysis. Producer defined A five digit integer. TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined An identifier in the format of XX## indicating a laboratory exposure to cobalt (XX=Co) or copper (XX=Cu) at a nominal concentration of ## micrograms per liter. SAMP_ID A unique identifier given to an individual tissue sample during experimentation. This is a key attribute that ties data among tables together. Producer defined A unique identifier given to an individual tissue sample during experimentation. SAMP_TYPE An identifier indicating whether the corresponding individual was analyzed for metals as an individual or as part of a pooled sample of multiple individuals of the same treatment for metals analysis. Individuals were pooled in order to reach a sufficient mass for metals analysis. Producer defined P Body burden analyzed as part of a pooled sample. Producer defined I Body burden analyzed for the individual. Producer defined ELEMENT The metal to which individual were exposed in the experiment. Producer defined Copper CASRN 7440-50-8 Producer defined Cobalt CASRN 1307-96-6 Producer defined NOMCONC The target concentration of the metal in the test chamber, calculated using the measured weight of the element and the volume of water into which it was diluted, reported in micrograms per liter. Producer defined 0.0 480.0 micrograms per liter MATRIX The type of sample analyzed using ICP-MS. Producer defined Tadpole tissue Tissue sample of tadpoles at a developmental stage before Gosner stage 46. Developmental stage was determined following the guidelines of Gosner (1960). Producer defined Metamorph tissue Tissue sample of metamorphs at a developmental stage at or above Gosner stage 46. Developmental stage was determined following the guidelines of Gosner (1960). Producer defined BID The block initiation date expressed in YYYYMMDD. A representation of time in which the smallest unit of measure is a day. This is a unique date assigned to a group or batch of samples for analysis or other processing to enable QA/QC traceability. Producer defined 20180928 20181004 YYYYMMDD ANALYTE The chemical constituent whose concentration was quantified in the corresponding observation. Producer defined Copper The 29th element on the periodic table. CAS number 7440-50-8. Producer defined Cobalt The 27th element on the periodic table. CAS number 7440-48-4. Producer defined VALUE The concentration of the ANALYTE measured in the in the corresponding sample. Producer defined 0.17 499.0 See UNITS attribute UNITS The standard unit of measure associated with the corresponding VALUE Producer defined A literal value representing the standard unit of measure. COMMENTS A description of other important information associated with the corresponding observation. Producer defined << empty cell >> No Data Producer defined A description of other important information associated with the corresponding observation. Co_Cu_MetamorphMass.csv Comma Separated Value (CSV) file containing the date at metamorphosis and mass of individual metamorphs in the chronic exposure assays of cobalt and copper. Producer defined TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined An identifier in the format of XX## indicating a laboratory exposure to cobalt (XX=Co) or copper (XX=Cu) at a nominal concentration of ## micrograms per liter. IND_ID A unique identifier given to an individual animal during experimentation. Producer defined An identifier for an individual in the format of XX### where XX indicates the element, cobalt (XX=Co) or copper (XX=Cu), and ### is a sequentially increase integer. ELEMENT The metal to which individual were exposed in the experiment. Producer defined Cobalt CASRN 1307-96-6 Producer defined Copper CASRN 7440-50-8 Producer defined NOMCONC The target concentration of the metal in the test chamber, calculated using the measured weight of the element and the volume of water into which it was diluted, reported in micrograms per liter. Producer defined 0.0 480.0 micrograms per liter REPLICATE A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. Producer defined A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. REMOVED_DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the individual reached Gosner stage 42 and was removed from the corresponding exposure treatment to complete metamorphosis. Producer defined 20180525 20180621 YYYYMMDD MORPH_DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the individual completed metamorphosis or Gosner stage 46. Producer defined 20180529 20180627 YYYYMMDD PARAMETER A literal value representing the parameter measured. Producer defined Mass The wet weight mass of the metamorph. This measurement was taken on the corresponding MORPH_DATE. Producer defined VALUE The analytical result for the parameter measured. Producer defined 0.23 0.59 See 'UNITS' attribute UNITS The standard unit of measure for the VALUE Producer defined A literal value representing the standard unit of measure. SAMP_ID A unique identifier given to an individual tissue sample during experimentation. This is a key attribute that ties data among tables together. Producer defined NA No Data. The sample was not measured for body burden and was not assigned a SAMP_ID. Producer defined A unique identifier given to an individual tissue sample during experimentation. SAMP_TYPE An identifier indicating whether the corresponding individual was analyzed for metals as an individual or as part of a pooled sample of multiple individuals of the same treatment for metals analysis. Individuals were pooled in order to reach a sufficient mass for metals analysis. Producer defined NA No Data. Body burden was not analyzed Producer defined P Body burden analyzed as part of a pooled sample. Producer defined Co_Cu_Survival2Metamorphosis.csv Comma Separated Value (CSV) file containing the number of individuals that survived as tadpoles at the end of the exposure period and the number of individuals that survived overall (tadpoles plus metamorphs) in the chronic exposure assays of cobalt and copper. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the observations were made. Producer defined 20180621 20180621 YYYYMMDD TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined An identifier in the format of XX## indicating a laboratory exposure to cobalt (XX=Co) or copper (XX=Cu) at a nominal concentration of ## micrograms per liter. ELEMENT The metal to which individual were exposed in the experiment. Producer defined Copper CASRN 7440-50-8 Producer defined Cobalt CASRN 1307-96-6 Producer defined NOMCONC The target concentration of the metal in the test chamber, calculated using the measured weight of the element and the volume of water into which it was diluted, reported in micrograms per liter. Producer defined 0.0 480.0 micrograms per liter REPLICATE A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. Producer defined A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. STOCKED The number of tadpoles introduced to the REPLICATE at the initiation of the experiment. Producer defined 5 5 count TAD_SURV The number of tadpoles that did not complete metamorphosis and survived to the end of the experiment. Producer defined 0 5 count MORPH_SURV The number of metamorphs (tadpoles that completed metamorphosis) that survived their exposure period. Producer defined 0 5 count OVR_SURV The total number of individuals from the number STOCKED that survived the exposure treatment in the corresponding REPLICATE. The total number of individuals includes tadpoles and metamorphs. Producer defined 3 5 count Co_Cu_TadpoleGD.csv Comma Separated Value (CSV) file containing the mass, snout-vent length, and Gosner stage of tadpoles at the end of the exposure period in the chronic exposure assays of cobalt and copper. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the observations were made. Producer defined 20180621 20180621 YYYYMMDD TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined An identifier in the format of XX## indicating a laboratory exposure to cobalt (XX=Co) or copper (XX=Cu) at a nominal concentration of ## micrograms per liter. IND_ID A unique identifier given to an individual animal during experimentation. This is a key attribute that ties data among tables together. Producer defined An identifier for an individual in the format of XX### where XX indicates the element, cobalt (XX=Co) or copper (XX=Cu), and ### is a sequentially increase integer. ELEMENT The metal to which individual were exposed in the experiment. Producer defined Cobalt CASRN 1307-96-6 Producer defined Copper CASRN 7440-50-8 Producer defined NOMCONC The target concentration of the metal in the test chamber, calculated using the measured weight of the element and the volume of water into which it was diluted, reported in micrograms per liter. Producer defined 0.0 480.0 micrograms per liter REPLICATE A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. Producer defined A numeric identifier used to distinguish the replicate testing chambers tested under identical conditions. Replicate measurements capture random biological variation. PARAMETER A literal value representing the parameter measured. Producer defined Mass The wet weight mass of the tadpole. Producer defined Snout-vent length The straight length measurement from the tip of the snout to the end of the cloaca. Producer defined Gosner stage The developmental stage of the tadpole at the time of the observation. Developmental stage was classified following Gosner (1960). Producer defined VALUE The analytical result for the parameter measured. Producer defined 0.14 41.0 See 'UNITS' attribute UNITS The standard unit of measure for the VALUE. Producer defined A literal value representing the standard unit of measure. SAMP_ID A unique identifier given to an individual tissue sample during experimentation. This is a key attribute that ties data among tables together. Producer defined NA No Data. The sample was not measured for body burden and was not assigned a SAMP_ID. Producer defined A unique identifier given to an individual tissue sample during experimentation. SAMP_TYPE An identifier indicating whether the corresponding individual was analyzed for metals as an individual or as part of a pooled sample of multiple individuals of the same treatment for metals analysis. Individuals were pooled in order to reach a sufficient mass for metals analysis. Producer defined NA No Data. Body burden was not analyzed. Producer defined P Body burden analyzed as part of a pooled sample. Producer defined I Body burden analyzed for the individual. Producer defined Co_Cu_TreatmentConc.csv Comma Separated Value (CSV) file containing the measured concentration of cobalt and copper found in the test water from the chronic exposure assays of cobalt and copper. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the samples were collected. Producer defined 20180501 20180621 YYYYMMDD CERC_ID A unique five digit integer used to identify an individual sample during ICP-MS analysis. Producer defined A five digit integer. TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined An identifier in the format of XX## indicating a laboratory exposure to cobalt (XX=Co) or copper (XX=Cu) at a nominal concentration of ## micrograms per liter. ELEMENT The metal to which individual were exposed in the experiment. Producer defined Cobalt CASRN 1307-96-6 Producer defined Copper CASRN 7440-50-8 Producer defined NOMCONC The target concentration of the metal in the test chamber, calculated using the measured weight of the element and the volume of water into which it was diluted, reported in micrograms per liter. Producer defined 0.0 480.0 micrograms per liter DAY The number of 24 hour days that have elapsed since the test commenced, representing when samples were collected. Producer defined 0 50 count MATRIX The type of sample analyzed using ICP-MS. Producer defined Water Treatment water used in the exposure. Producer defined BID The block initiation date expressed in YYYYMMDD. A representation of time in which the smallest unit of measure is a day. This is a unique date assigned to a group or batch of samples for analysis or other processing to enable QA/QC traceability. Producer defined 20180514 20180708 YYYYMMDD ANALYTE The chemical constituent whose concentration was quantified in the corresponding observation. Producer defined Cobalt CASRN 1307-96-6 Producer defined Copper CASRN 7440-50-8 Producer defined LOQ A numeric value for the limit of quantification of the corresponding ANALYTE. The limit of quantification may increase if sample dilution is required due to column overload and peak tailing or fronting. Producer defined 0.2 1.0 See UNITS attribute VALUE The concentration of the ANALYTE in the corresponding observation. Producer defined LTLOQ The measured value was less than the limit of quantification. Producer defined 0.56 433.0 See UNITS attribute UNITS The standard unit of measure associated with the corresponding VALUE and LOQ. Producer defined The standard unit of measure associated with the corresponding VALUE and LOQ. Co_Cu_WQ.csv Comma Separated Value (CSV) file containing the dissolved oxygen, temperature, specific conductance, alkalinity, pH, hardness, and ammonia concentration of test water in the chronic exposure assays of cobalt and copper. Producer defined DATE A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the samples were collected. Producer defined 20180501 20180621 YYYYMMDD TRT_ID An identifier for the treatment of the corresponding observation. This is a key attribute that ties data among tables together. Producer defined An identifier in the format of XX## indicating a laboratory exposure to cobalt (XX=Co) or copper (XX=Cu) at a nominal concentration of ## micrograms per liter. ELEMENT The metal to which individual were exposed in the experiment. Producer defined Copper CASRN 7440-50-8 Producer defined Cobalt The metal to which individual were exposed in the experiment. Producer defined NOMCONC The target concentration of the metal in the test chamber, calculated using the measured weight of the element and the volume of water into which it was diluted, reported in micrograms per liter. Producer defined 0.0 480.0 micrograms per liter PARAMETER A literal value representing the parameter measured. Producer defined Dissolved oxygen Concentration of gaseous oxygen in water or water solutions, unfiltered, measured as milligrams per liter. Producer defined Temperature Measure of the thermal energy present in water, reported in degrees Celsius. Producer defined Specific conductance Reciprocal of the resistance in ohms measured between opposite faces of a centimeter cube of an aqueous solution at a specified temperature, 25 degrees Celsius, microsiemens per centimeter. Producer defined pH Logarithm of the reciprocal hydrogen ion concentration in atoms per liter or acidity of water in test chambers. Producer defined Alkalinity The total titratable bases in water or buffering capacity of culture and test waters as equivalent milligrams of calcium carbonate per liter. Producer defined Hardness Alkaline salts in water, mainly calcium and magnesium, as milligrams calcium carbonate per liter. Producer defined Ammonia Concentration of ammonia in the sample, reported as milligrams per liter as total nitrogen. Producer defined VALUE The analytical result of the parameter measured. Producer defined 0.0 710.0 See 'UNITS' attribute UNITS The standard unit of measure associated with the corresponding VALUE. Producer defined The standard unit of measure associated with the corresponding VALUE. GS ScienceBase U.S. Geological Survey mailing and physical

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