Shannon L. White Justin M. Waraniak Luke R. Iwanowicz Tyler Wagner 20250711 spreadsheet Reston, VA U,S, Geological Survey https://doi.org/10.5066/P1C8EP3V This dataset contains gene expression data for brook trout (Salvelinus fontinalis) collected from the Loyalsock Creek watershed in Pennsylvania. Gene expression was quantified for three genes of interest: elongation factor 1-alpha (EF1), SfontSerpinh1 (a heat shock protein gene), and SfontColdRNA (a gene associated with cold response). The dataset was generated to support investigations into both individual-level variation and inter-population differences in gene expression among brook trout inhabiting coldwater stream environments. Tissue samples were collected from multiple individuals across distinct sites within the watershed, and quantitative PCR (qPCR) was used to assess relative gene expression levels. These data can be used to explore patterns of transcriptomic plasticity and potential local adaptation in response to environmental variation across the Loyalsock Creek system. Data were collected to quantify individual- and population-level variation in gene expression profiles. 20160512 20170413 ground condition Complete None planned Loyalsock Creek watershed, Pennsylvania -76.60688 -76.51033 41.46914 41.42714 ISO 19115 Topic Category biota None brook trout gene expression transcriptomics Brook trout transcriptomics brook trout gene expression transcriptomics USGS Metadata Identifier USGS:686fd0afd4be025ce526c366 None Loyalsock Creek Pennsylvania Brook trout brook trout Life Life No access constraints. Please see 'Distribution Information' for details. These data are marked with a Creative Common CC0 1.0 Universal License. These data are in the public domain and do not have any use constraints. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Shannon White U.S. Geological Survey mailing

11649 Leetown Rd

Kearneysville wv 25430 (304) 725-8461 slwhite@usgs.gov Data are accurate. Multiple spot checks of data sets, instrument calibrations, and inclusion of positive controls were performed. Yes Data set is considered complete for the information presented as described in the abstract. Tissue samples consisted of gill biopsies taken from brook trout within 5 minutes of capture via electrofishing. Temperature data were derived from continuous temperature loggers placed in the stream. All gene expression data were generated via qPCR. 20250701 gene expression data.csv Comma Separated Value (CSV) file containing data. Producer Defined Location General location of fish capture Producer Defined Downstream Shanerburg Run Fish captured in the lower reach of Shanerburg Run Producer defined Upstream Shanerburg Run Fish captured in the upper reach of Shanerburg Run Producer defined Double Run Fish captured in middle section of Double RUn Producer defined Downstream Pole Bridge Run Fish captured in the lower reach of Pole Bridge Run Producer defined Upstream Pole Bridge Run Fish captured in the upper reach of Pole Bridge Run Producer defined East Branch Fish captured in East Branch Producer defined Downstream Double Run Fish captured in the lower section of Double Run Producer defined Upstream Double Run Fish captured in the upper reach of Double Run Producer defined Pole Bridge Fish captured in the middle section of Pole Bridge Run Producer defined Mainstem Fish captured in mainstem Loyalsock Creek Producer defined Mainstream Stream a fish was captured in Producer Defined Shanerburg Run Fish captured in Shanerburg Run Producer defined Double Run Fish captured in Double Run Producer defined Pole Bridge Run Fish captured in Pole Bridge Run Producer defined East Branch Fish captured in East Branch Producer defined Mainstem Fish captured in mainstem Loyalsock Creek Producer defined Date Date a fish was sampled Producer Defined Dates TL (mm) Fish total length, measured in mm Producer Defined 106 262 mm Weight (g) Fish weight Producer Defined 9.9 194.5 g Final_ID Unique identifying number for reach fish Producer Defined NA No Data Producer defined Unique identifying number for reach fish Site_FishID Unique ID for each fish that also includes a site ID Producer Defined NA No Data Producer defined Unique ID for each fish that also includes a site ID SampleTime2 Time a fish was sampled Producer Defined 800 1755 EF1 Elongation factor 1 expression value Producer Defined << empty cell >> No Data Producer defined 137.06 14,732.41 SfontSerpinh1 SfontSerpinh1 expression value Producer Defined << empty cell >> No Data Producer defined 4.631 15,026.24 SfontColdRNA Cold-inducible RNA expression value Producer Defined << empty cell >> No Data Producer defined 158.43 9,609.65 NormSfontSerpinh1 Normalized NormSfontSerpinh1 expression value Producer Defined NA No Data Producer defined 0.011050208 2.818173186 NormSfontColdRNA Normalized cold-inducible RNA value Producer Defined NA No Data Producer defined 0.096082386 51.22344571 lnNormSfontSerpinh1 Log base 2 normalized NormSfontSerpinh1 expression value Producer Defined NA No Data Producer defined -6.499782638 1.494760273 lnNormSfontColdRNA Log base 2 normalized cold-inducible RNA value Producer Defined NA No Data Producer defined -3.379584212 5.678732399 newTime Field that combines sample date and time Producer Defined Date and time month Sample month Producer Defined 3 11 day Numeric day of sampling Producer Defined 2 30 year Sample year Producer Defined 2016 2017 season Sample season Producer Defined spring Sampling occured in March, April, or May. Producer defined summer Sampling occured in June or July Producer defined fall Sampling occured in September or November Producer defined newLocation Abbreviation for sample site Producer Defined shaner Sampling occured in Shanerburg Run Producer defined double Sampling occured in Double Run Producer defined pole Sampling occured in Pole Bridge Run Producer defined east Sampling occured in East Branch Producer defined meanTCap Mean temperature for 1 hour prior to capture Producer Defined 0.246 18.271 sdTCap Standard deviation in temperature 1 hour prior to capture Producer Defined 0.0 1.226117993 maxTCap Maximum temperature 1 hour prior to capture Producer Defined 0.246 18.271 minTCap Minimum temperature 1 hour prior to capture Producer Defined 0.135 18.2 meanT1d Mean temperature for 24 hours prior to capture Producer Defined 0.060061224 17.91695918 sdT1d Standard deviation in temperature 24 hours prior to capture Producer Defined 0.087929045 1.88526645 maxT1d Maximum temperature 24 hours prior to capture Producer Defined 0.273 18.509 minT1d Minimum temperature 24 hours prior to capture Producer Defined -0.004 17.368 meanT2d Mean temperature for 48 hours prior to capture Producer Defined -0.018572165 18.04174227 sdT2d Standard deviation in temperature 48 hours prior to capture Producer Defined 0.180567241 2.093909417 maxT2d Maximum temperature 48 hours prior to capture Producer Defined 0.3425 19.032 minT2d Minimum temperature 48 hours prior to capture Producer Defined -0.704 17.201 meanT3d Mean temperature for 72 hours prior to capture Producer Defined 0.124872414 18.27173103 sdT3d Standard deviation in temperature 72 hours prior to capture Producer Defined 0.352691751 2.456966335 maxT3d Maximum temperature 72 hours prior to capture Producer Defined 1.588 20.412 minT3d Minimum temperature 72 hours prior to capture Producer Defined -0.704 17.201 meanT4d Mean temperature for 96 hours prior to capture Producer Defined 0.612220207 18.16650777 sdT4d Standard deviation in temperature 96 hours prior to capture Producer Defined 0.446703916 2.448874282 maxT4d Maximum temperature 96 hours prior to capture Producer Defined 3.749 20.412 minT4d Minimum temperature 96 hours prior to capture Producer Defined -0.704 17.201 meanT5d Mean temperature for 120 hours prior to capture Producer Defined 1.616487552 18.00812033 sdT5d Standard deviation in temperature 120 hours prior to capture Producer Defined 0.486987151 2.57518781 maxT5d Maximum temperature 120 hours prior to capture Producer Defined 6.077 20.412 minT5d Minimum temperature 120 hours prior to capture Producer Defined -0.704 16.915 deltaTCap Difference in maximum and minimum temperature in the one hour prior to capture Producer Defined 0.0 2.316 deltaT1d Difference in maximum and minimum temperature in the 24 hours prior to capture Producer Defined 0.277 6.042 deltaT2d Difference in maximum and minimum temperature in the 48 hours prior to capture Producer Defined 0.692 7.73 deltaT3d Difference in maximum and minimum temperature in the 72 hours prior to capture Producer Defined 1.71 9.747 deltaT4d Difference in maximum and minimum temperature in the 96 hours prior to capture Producer Defined 1.71 9.747 deltaT5d Difference in maximum and minimum temperature in the 120 hours prior to capture Producer Defined 2.117 9.747 U.S. Geological Survey - ScienceBase mailing address

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Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P1C8EP3V None 20250711 Shannon White mailing

11649 Leetown Road

Kearneysville WV 25430 (304) 725-8461 slwhite@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999