Robert D. Hunter Matthew R. Acre Ryan Brown Ryan Young Robert Mapes Jessica L. Bowser 20250421 spreadsheet Reston, VA U.S. Geological Survey [(Robert D. Hunter)(U.S. Geological Survey, Great Lakes Science Center, Lake Erie Biological Station)(https://orcid.org/0000-0002-6021-4479)]: [(Matthew R. Acre)(U.S. Geological Survey, Columbia Environmental Research Center)(https://orcid.org/0000-0002-5417-9523)]: [(Ryan Brown)(Michigan Department of Natural Resources, Fisheries Division)( https://orcid.org/0000-0003-0147-7831)]: [(Ryan Young)(U.S. Fish and Wildlife Service)(https://orcid.org/0000-0002-6967-4260)]: [(Robert Mapes)(University of Toledo, Department of Environmental Sciences, Lake Erie Center)(https://orcid.org/0009-0004-9946-4274)]: [(Jessica L. Bowser)(U.S. Fish and Wildlife Service)( https://orcid.org/0000-0002-8466-5362)]: https://doi.org/10.5066/P14DGNFN Robert D. Hunter Song S. Qian Jason L. Fischer Ryan Brown Lucas Nathan John M. Dettmers James J. Roberts Corbin D. Hilling Matthew R. Acre Robert Mapes Ryan Young Christine M. Mayer 202505 publication Fisheries Research vol. 285 n/a Elsevier BV ppg. 107344 https://doi.org/10.1016/j.fishres.2025.107344 Data were collected during efforts to remove invasive grass carp (Ctenopharyngodon idella) from the Sandusky River, OH, USA during 2020–2023. The data includes the number of grass carp captured, capture method, total vessel hours, removal effort year, removal effort month, number of non-target species captured, and river kilometer (distance from the river mouth measured in 0.5 kilometer units). Data include non-spawning grass carp removal efforts from the lower 24 kilometers of the Sandusky River using electrofishing and combined (i.e., electrofishing around a trammel net) removal methods when vessels were working independently. Data were collected to document grass carp (Ctenopharyngodon idealla) removals from the Sandusky River, OH, USA during 2020–2023. The data can be used to compare capture efficiency (per vessel hour and per removal effort) between the electrofishing only method, and electrofishing in combination with a trammel net. Information about the year, month, and river kilometer (distance from the river mouth measured in 0.5 km units) can be used to account for spatial and temporal trends in capture. 202006 202310 See Supplemental Info Complete None planned Sandusky River, OH, USA -83.1339 -82.9763 41.4715 41.3382 ISO 19115 Topic Category transportation biota environment USGS Thesaurus invasive species None trammel-netting electrofishing capture method grass carp USGS Metadata Identifier USGS:67dc37a3d34eae450ac1c3df Geographic Names Information System (GNIS) Sandusky River Ohio Sandusky County Integrated Taxonomic Information System (ITIS) grass carp Kingdom Animalia animals Subkingdom Bilateria triploblasts Infrakingdom Deuterostomia Phylum Chordata chordates Subphylum Vertebrata vertebrates Infraphylum Gnathostomata Superclass Actinopterygii ray-finned fishes spiny rayed fishes Class Teleostei Superorder Ostariophysi Order Cypriniformes minnows suckers Superfamily Cyprinoidea Family Cyprinidae carps minnows carps and minnows Genus Ctenopharyngodon grass carps Species Ctenopharyngodon idella grass carp silver orfe TSN: 163537 No access constraints. No use constraints. Robert D Hunter USGS - MIDCONTINENT REGION Fishery Biologist mailing and physical

Huron Lake Erie Biological Station,Huron Lake Erie Biological Station

Huron OH 44839 419-616-0060 rhunter@usgs.gov We would like to thank the hardworking crews who collected the data from University of Toledo; U.S. Fish and Wildlife Service; U.S. Geological Survey; and Michigan Department of Natural Resources. Support for this research came from the Great Lakes Fishery Commission and the Great Lakes Restoration Initiative administered by the Ohio Department of Natural Resources Division of Wildlife. Robert D. Hunter Song S. Qian Jason L. Fischer Ryan Brown Lucas Nathan John M. Dettmers James J. Roberts Corbin D. Hilling Matthew R. Acre Robert Mapes Ryan Young Christine M. Mayer 202505 publication Fisheries Research vol. 285 n/a Elsevier BV ppg. 107344 https://doi.org/10.1016/j.fishres.2025.107344 Values reported here contain counts of fish that were captured and handled following the protocols outlined in the University of Toledo, Institutional Animal Care and Use Committee 108727–04 - Great Lakes Fish Population Assessments and in accordance with agency specific protocols. Bycatch counts were done once in the field and bycatch were immediately released to reduce handling time. Bycatch ranges were used to assess accuracy as described below. Tabular errors may have been possible during bycatch counts, but care was taken to minimize risk. Captured grass carp were either harvested or tagged and released. Because of the additional handling time, grass carp counts were confirmed during multiple data recording steps. Date and time information was recorded using Survey123 or boat mounted gps units. Date and time information was reviewed by field crews following sampling and confirmed during quality control steps that took place prior to data download. All values in this dataset were confirmed by quality controls steps that were performed prior to data download. Additional quality control steps are briefly mentioned here. Grass carp captures numbers were between 0–5 with most efforts resulting in zero capture. Values for total grass carp captures were within expected values. Bycatch values were mean 12.13, range = 0–282. Values for bycatch were within expected values based on reported observations in the field. Methods included electrofishing only and electrofishing combined with trammel nets. All efforts involved electrofishing. Combined efforts were confirmed to have information about an associated trammel net set or reported bycatch (only occurred with trammel nets) numbers. Years fell between expected year ranges for the study, 2020–2023. Months fell between expected month ranges for the study sampling period, 4–11 during 2020–2023. Hours (difference between start and end time in numeric hours) was between expected values based on observations in the field, mean 0.921, range = 0.0167–5.733. Any effort values >8 hours were filtered from the dataset because such values did not occur during the course of normal sampling. Such values likely represented errors in start or end times or a major deviation from removal protocols. Values above 3 hours were rare but occasionally were observed when trammel nets became entangled on debris in the river. River kilometers were within expected ranges mean 14.968, range = 0–24. Associated grass carp captures and efforts were confirmed to have occurred in the study area during topology quality control checks performed prior to download. No duplicated rows were found in the data after it was checked for duplicates using the duplicated() function in R. Data included in this report contain information about non-spawning grass carp removal efforts in the lower 24 km of the Sandusky River, OH, USA during 2020–2023 using non-spawning, electrofishing or electrofishing combined with a trammel net by one vessel. Removal efforts that took over 8 hours to complete were considered major deviations from protocol or inaccurate and were removed from consideration. Data about additional removal efforts, locations, or time periods associated are collected by partner agencies and can be requested from the Grass Carp Advisory Committee (GCAC) that supports "signatory agencies to A Joint Strategic Plan for Management of Great Lakes Fisheries (Joint Strategic Plan), the Council of Lake Committees and the Great Lakes Fishery Commission". not applicable not applicable Andrew S. Briggs Jan C. Dean James C. Boase Patrick M. Kocovsky James A. Luoma 20190614 publication North American Journal of Fisheries Management vol. 39, issue 4 n/a Oxford University Press (OUP) ppg. 705-713 https://doi.org/10.1002/nafm.10303 Digital and/or Hardcopy 20190614 publication date Briggs et al. 2019 Briggs et al. (2019) recommended electrofishing settings Ryan T. Young James C. Boase John T. Buszkiewicz Jan C. Dean Jesse T. McCarter 20230413 publication North American Journal of Fisheries Management vol. 43, issue 3 NA Oxford University Press (OUP) ppg. 859-868 https://doi.org/10.1002/nafm.10899 Digital and/or Hardcopy 20230413 publication date Young et al. (2023) Young et al. (2023) recommended electrofishing settings. Grass carp removal efforts: Removal crews from the University of Toledo, U.S. Fish and Wildlife Service, Ohio Department of Natural Resources, Michigan Department of Natural Resources, and U.S. Geological Survey used netting and boat electrofishing to capture and remove invasive Grass Carp from waters and tributaries of Lake Erie during 2020–2023. Removal crews used electrofishing only and electrofishing in combination with trammel nets to capture invasive grass carp. Electrofishing was done with a single vessel using settings described by Young et al. (2023) and Briggs et al. (2019). The combination method used electrofishing in combination with a 182 x 4 m (length x depth) trammel net with 10.2 cm monofilament and 45.7 cm square twisted multifilament nylon mesh panel trammel net. Effort and catch information were recorded by removal crews on paper datasheets that were uploaded in Survey123 after quality control, or electronically in Survey123 and uploaded to ArcGIS Online following quality control. Additional quality control steps were performed semi-annually following upload to ArcGIS Online. 20240828 Data were downloaded from ArcGIS Online on August 28, 2024. Data were filtered in R version 4.4.1 to include only non-spawning removal methods completed by a single vessel that used electrofishing only or the combination method in the lower 24 km of the Sandusky River, OH, USA to capture grass carp during 2020–2023. Removal efforts that reported over 8 hours of effort were also removed from the dataset because recorded times were likely inaccurate or represent a major deviation from standard protocols. 20240828 Robert D Hunter USGS - MIDCONTINENT REGION Fishery Biologist mailing and physical

Lake Erie Biological Station, 380 Huron St.

Huron OH 44839 419-616-0060 rhunter@usgs.gov MethodCompare_28Aug2024_Data.csv Comma Separated Value (CSV) file containing data. Producer Defined method Removal method used. Producer Defined Combination Solo Combination Solo efforts used a single vessel to electrofish in combination with a trammel net in the littoral zone to capture grass carp. Trammel nets were 182 x 4 m (length x depth) trammel net with 10.2 cm monofilament and 45.7 cm square twisted multifilament nylon mesh panels. Electrofishing used settings described by Young et al. (2023) and Briggs et al. (2019). Producer defined E-fishing Solo Electrofishing only used a single vessel to electrofish the littoral zone to capture grass carp. Electrofishing used settings described by Young et al. (2023) and Briggs et al. (2019). Producer defined year Year during which a removal method occurred. Producer Defined 2020 2023 year 1 month Month during which a removal method occurred. Producer Defined 4 11 month 1 rkm The river kilometer (distance from the river mouth measured in 1 km units) in which a removal effort occurred. Producer Defined 0 24 kilometer 1 bycatch The count of non-target species that were captured during a removal effort. Producer Defined 0 282 fish 1 hours The total number of hours required to complete a removal effort (e.g., difference between the start time and end time). Hours for electrofishing only were calculated by recording a start time, electrofishing including active shocking and boat positioning time, and recording the end time. The difference between start and end times is hours. For the combined method hours was calculated by recording a start time, setting the trammel net, electrofishing including active shock time and boat positioning time, retrieving the net, processing catch, and recording an end time. Hours was the difference between start and end time. Producer Defined 0.016666667 5.733333333 hours total_grass_carp The numeric count of grass carp captured during a removal effort. Producer Defined 0 5 grass carp 1 U.S. Geological Survey - ScienceBase mailing address

Denver Federal Center

Building 810

Mail Stop 302

Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Digital Data https://doi.org/10.5066/P14DGNFN None 20250430 Great Lakes Science Center mailing and physical

1451 Green Road

Ann Arbor MI 48105 US 734-994-3331 GS_ASK_GLSC@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999