Robert Peck Dan Mikros Ellen Dunkle Kylle Roy 20250422 tabular digital data https://doi.org/10.5066/P14EOVKX Robert W. Peck Dan Mikros Ellen J. Dunkle Kelly Jaenecke Kylle Roy 20250811 publication Agricultural and Forest Entomology vol. 28, issue 1 n/a Wiley ppg. 49-60 https://doi.org/10.1111/afe.70007 This data release includes metadata and tabular data that describes ambrosia beetles that emerged from galleries within trees inoculated with Ceratocystis lukuohia. Beetles were collected in boring dust traps (BDTs) that were placed over the entrances of active galleries. The dataset includes beetles identified by species and the date on which they collected. The purpose of this dataset is to identify the beetle species attacking ohia inoculated with Ceratocystis lukuohia at each site and to determine the length of time that galleries remained active. 20190404 20210813 observed Complete None planned East side, Island of Hawaiʻi -155.3838 -154.9525 19.7525 19.4044 ISO 19115 Topic Category biota None Rapid Ohia Death fungal disease Ceratocystis Ambrosia beetles USGS Metadata Identifier USGS:67d3137dd34e1acf3979cebc None Island of Hawaii None Metrosideros polymorpha Xyleborus ferrugineus Xyleborus simillimus Ceratocystis Ceratocystis lukuohia Domain Eukaryota Kingdom Plantae Subkingdom Viridiplantae Infrakingdom Streptophyta Superdivision Embryophyta Division Tracheophyta Subdivision Spermatophytina Class Magnoliopsida Superorder Rosanae Order Myrtales Family Myrtaceae Genus Metrosideros Species Metrosideros polymorpha TSN: 27259 Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda Class Insecta Subclass Pterygota Infraclass Neoptera Superorder Holometabola Order Coleoptera Suborder Polyphaga Infraorder Cucujiformia Superfamily Curculionoidea Family Curculionidae Subfamily Scolytinae Genus Xyleborus Species Xyleborus ferrugineus TSN: 188145 Species Xyleborus simillimus TSN: 188162 Kingdom Fungi Subkingdom Dikarya Division Ascomycota Subdivision Pezizomycotina Class Sordariomycetes Subclass Sordariomycetidae Order Ophiostomatales Family Ophiostomataceae Genus Ceratocystis TSN: 181740 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Robert W Peck Hawaii Cooperative Studies Unit, University of Hawaii at Hilo Research Specialist physical

Bldg 344 Chain of Craters Road

Hawaii National Park HI 96718 USA 808-210-6141 808-967-8568 bwpeck@usgs.gov We thank Theo Black and Josie Tupu for help in the field. We also thank Dustin Swan of the Big Island Invasive Species Committee for felling and sectioning trees. J.B. Friday and Susan Miyasaka generously provided laboratory space at the University of Hawaii at Manoa Komohana Research and Extension Center. Hawaii Department of Land and Natural Resources invertebrate collection and state forest access permits were issued by Cynthia King and Steve Bergfeld, respectively. We thank Craig Blaisdell for his continued support of ROD studies and access to the Keaukaha Military Reservation. This study was supported by U.S.D.A. Forest Service, Hawaii Division of Forestry and Wildlife, and the U.S. Geological Survey Biological Threats and Invasive Species Program. Windows 10, Excel 365 ProPlus No formal attribute accuracy tests were conducterd. Beetles were identified using a taxonomic key and be comparison to reference specimens. All data are logical, consistent, and falls within expected ranges. All data were checked for duplications and omissions. Dataset is complete and represent results obtained at the three study sites on the Island of Hawaii during 4 April 2019 - 3 August 2021. NA NA Within one to three weeks of beetles starting to attack inoculated trees, beetle boring dust traps (BDTs) were placed over a subset of all active galleries to collect extruded frass and beetles that emerged from the galleries over time. BDTs consisted of 60 ml screw-capped clear plastic vials that were painted white, except for an unpainted 1.5 cm wide longitudinal strip that that allowed visual inspection of the vial. A 6 mm hole was drilled in the center of each cap. Before the vial was capped, the cap was placed over the gallery so that the hole aligned with the gallery entrance. The cap was then secured to the tree using two 1 cm long screws drilled through the cap and into surface of the bark. Fast-drying silicone caulk was applied to the outer surface of the cap about 30 seconds before attaching it to the tree to help secure the trap to the tree and to prevent rainwater from entering the trap. Once the cap was secured to the tree, the vial was screwed onto the cap and the BDT was complete. Frass and beetles were removed from BDTs using alcohol and flame-sterilized forceps and placed into sterile 1.5 ml microcentrifuge tubes. Beetles collected in BDTs were archived for identification. Typically, three BDTs were placed over galleries on each tree, but as many as 10 BDTs were placed on some trees. 20190404 Robert Peck physical

Bldg 344 Crater Rim Drive

Hawaii National Park HI 96718 808-210-6141 Data were entered into spreadsheets and proofed for accuracy. 20211230 ROD beetles in boring dust traps.csv Comma Separated Value (CSV) file containing data. Producer Defined Beetle ID Unique number assigned to each beetle collected in a BDT (boring dust trap) Producer Defined 1706 3949 Site Name of each study site Producer Defined KMR Keaukaha Military Reservation Producer defined OLA Olaa Forest Reserve Producer defined WAI Waiakea Forest Reserve Producer defined Tree number Unique number assigned to each tree on each study site Producer Defined 1 9 BDT number Unique number assigned to each BDT (boring dust trap) on each tree at each study site Producer Defined 1 10 Installation date Date that a BDT (boring dust trap) was installed over a beetle gallery on a tree Producer Defined n/a No Data Producer defined 04/04/19 12/30/19 Collection date Date that a beetle was collected from a BDT (boring dust trap) Producer Defined n/a No Data Producer defined 05/15/19 08/03/21 Beetle species Species of beetle collected in a BDT (boring dust trap) Producer Defined Xyleborus ferrugineus Species Xyleborus ferrugineus Producer defined Xyleborinus saxesenii Species Xyleborinus saxesenii Producer defined Xyleborus simillimus Species Xyleborus simillimus Producer defined Number of beetles Number of individual beetles collected from a BDT (boring dust trap) on a particular date Producer Defined 1 8 U.S. Geological Survey GS ScienceBase mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes and does not imply endorsement by the U.S. Government. Digital Data https://doi.org/10.5066/P14EOVKX None 20260224 U.S. Geological Survey - PIERC Data Steward physical

Bldg 344 Crater Rim Drive

Hawaii National Park HI 96718 USA 808-210-6141 pierc-datasteward@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999