Robert Peck Dan Mikros Ellen Dunkle Kylle Roy 20250422 tabular digital data https://doi.org/10.5066/P14EOVKX Robert W. Peck Dan Mikros Ellen J. Dunkle Kelly Jaenecke Kylle Roy 20250811 publication Agricultural and Forest Entomology vol. 28, issue 1 n/a Wiley ppg. 49-60 https://doi.org/10.1111/afe.70007 This data release includes metadata and tabular data that describes ambrosia beetles that were collected on sticky traps placed on trees inoculated with Ceratocystis lukuohia and on control trees which were not inoculated with the fungus. The dataset includes beetles identified by species and the date on which they were collected. Two sticky traps were placed on each tree. The purpose of this dataset is to identify the ambrosia beetle species that land on infected and uninfected ohia at each site. These data aim to help determine the beetle community at each site and complement data collected from BDTs (boring dust traps) that identified beetle species attacking ohia. 20190114 20210812 observed Complete None planned East side, Island of Hawaiʻi -155.3838 -154.9525 19.7525 19.4044 ISO 19115 Topic Category biota None Rapid Ohia Death fungal disease Ceratocystis Ambrosia beetles USGS Metadata Identifier USGS:67d312fcd34e1acf3979ceae None Island of Hawaii None Metrosideros polymorpha Xyleborus ferrugineus Xyleborus simillimus Xyleborus perforans Xyleborus affinis Ceratocystis Ceratocystis lukuohia Domain Eukaryota Kingdom Plantae Subkingdom Viridiplantae Infrakingdom Streptophyta Superdivision Embryophyta Division Tracheophyta Subdivision Spermatophytina Class Magnoliopsida Superorder Rosanae Order Myrtales Family Myrtaceae Genus Metrosideros Species Metrosideros polymorpha TSN: 27259 Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda Class Insecta Subclass Pterygota Infraclass Neoptera Superorder Holometabola Order Coleoptera Suborder Polyphaga Infraorder Cucujiformia Superfamily Curculionoidea Family Curculionidae Subfamily Scolytinae Genus Xyleborus Species Xyleborus ferrugineus TSN: 188145 Species Xyleborus simillimus TSN: 188162 Species Xyleborus perforans TSN: 188158 Species Xyleborus affinis TSN: 188140 Kingdom Fungi Subkingdom Dikarya Division Ascomycota Subdivision Pezizomycotina Class Sordariomycetes Subclass Sordariomycetidae Order Ophiostomatales Family Ophiostomataceae Genus Ceratocystis TSN: 181740 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Robert W Peck Hawaii Cooperative Studies Unit, University of Hawaii at Hilo Research Specialist physical

Bldg 344 Chain of Craters Road

Hawaii National Park HI 96718 USA 808-210-6141 808-967-8568 We thank Theo Black and Josie Tupu for help in the field. We also thank Dustin Swan of the Big Island Invasive Species Committee for felling and sectioning trees at SBK. J.B. Friday and Susan Miyasaka generously provided laboratory space at the University of Hawaii at Manoa Komohana Research and Extension Center. Hawaii Department of Land and Natural Resources invertebrate collection and state forest access permits were issued by Cynthia King and Steve Bergfeld, respectively. We thank Craig Blaisdell for his continued support of ROD studies and access to the Keaukaha Military Reservation. This study was supported by U.S.D.A. Forest Service, Hawaii Division of Forestry and Wildlife, and the U.S. Geological Survey Biological Threats and Invasive Species Program. Windows 10, Excel 365 ProPlus No formal attribute accuracy tests were conducterd. Beetles were identified using a taxonomic key and be comparison to reference specimens. Xyleborus perforans and Xyleborus affinis look very similar and could not be differentiated due to the sticky substance associated with the trap. Therefore, representatives of this species was named Xyleborus perforans/affinis in the dataset. All data are logical, consistent, and falls within expected ranges. All data were checked for duplications and omissions. Dataset is complete and represent results obtained at the three study sites on the Island of Hawaii during 10 January 2019 - 18 August 2021. NA NA The community of ambrosia beetles attracted to inoculated trees was assessed over time using sticky cards stapled to the trees. Sticky cards consisted of 21.6 x 27.9 cm transparent plastic sheets (Apollo Transparency Film, ACCO Brands, Lake Zurich, Illinois) onto which a thin layer of sticky resin (Tree Tanglefoot Insect Barrier, The Scotts Company, Marysville, OH) was applied to the outward facing surface. One sticky card was stapled 25 cm below each of the two inoculation points on each treated tree, and at similar locations on control trees. Ambrosia beetles collected on the traps were removed using sterilized forceps and placed into 1.5 ml microcentrifuge tubes containing 70% isopropyl alcohol and returned to the lab for identification. Sticky cards were placed on treated trees several days after inoculation. Sticky cards were changed periodically as they accumulated debris and the sticky resin became dry. 20190114 Robert Peck physical

Bldg 344 Crater Rim Drive

Hawaii National Park HI 96718 808-210-6141 Data were entered into spreadsheets and proofed for accuracy. 20201230 ROD beetles from sticky traps.csv Comma Separated Value (CSV) file containing data. Producer Defined Site Name of each study site Producer Defined KMR Keaukaha Military Reservation Producer defined OLA Olaa Forest Reserve Producer defined WAI Waiakea Forest Reserve Producer defined Tree number Unique number assigned to each tree on each study site Producer Defined 1 9 Card Unique letter assigned to each sticky trap placed on each tree at each study site Producer Defined A Indicates sticky trap A Producer defined B Indicates sticky trap B Producer defined Installation date Date that a sticky trap was installed on a tree Producer Defined 1/14/2019 Sticky traps installed 14 January 2019 Producer defined 4/11/2019 Sticky traps installed 11 April 2019 Producer defined 6/13/2019 Sticky traps installed 13 June 2019 Producer defined 6/19/2019 Sticky traps installed 19 June 2019 Producer defined 4/12/2019 Sticky traps installed 12 April 2019 Producer defined 4/5/2019 Sticky traps installed 5 April 2019 Producer defined Collection date Date that beetles were collected from a sticky trap Producer Defined 1/24/2019 8/12/2021 XylFer Number of individuals of beetle species Xyleborus ferrugineus collected on sticky card. XylFer is a unique code used to represent this species. Producer Defined 0 19 XylPer/Aff Number of individuals of beetle species Xyleborus perforans or Xyleborus affinis collected on sticky card. XylPer/Aff is a unique code used to represent these two species. Producer Defined 0 6 XylSim Number of individuals of beetle species Xyleborus simillimus collected on sticky card. XylSim is a unique code used to represent this species. Producer Defined 0 6 XylSax Number of individuals of beetle species Xyleborinus saxesenii collected on sticky card. XylSax is a unique code used to represent this species. Producer Defined 0 67 XylAnd Number of individuals of beetle species Xyleborinus andrewsi collected on sticky card. XylAnd is a unique code used to represent this species. Producer Defined 0 3 XylSp Number of individuals of beetle species Xyleborus sp. collected on sticky card. XySp is a unique code used to represent this undetermined species. The particular species could not be determined. Producer Defined 0 6 U.S. Geological Survey GS ScienceBase mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes and does not imply endorsement by the U.S. Government. Digital Data https://doi.org/10.5066/P14EOVKX None 20260224 U.S. Geological Survey - PIERC Data Steward physical

Bldg 344 Crater Rim Drive

Hawaii National Park HI 96718 USA 808-210-6141 pierc-datasteward@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999