Robert J Dusek Hon Ip 20250314 spreadsheet https://doi.org/10.5066/P13E4DAC Collection data for rabbit tissues sequenced for metagenomic evaluation of selected tissues. Specimens were submitted for diagnostic testing to determine rabbit hemorrhagic disease 2 presence and/or determine cause of death. Data can be used to examine species and geographic relationships of results following metagenomic analysis of tissues. 20200605 20210512 ground condition Complete None planned Contiguous United States -127.2656 -65.5664 49.4967 24.0465 ISO 19115 Topic Category biota None lagomorpha rabbit cottontail USGS Thesaurus genetics wildlife disease USGS Metadata Identifier USGS:67cb332fd34ed373770989ae None United States of America Arizona California Iowa Massachusetts Montana Nevada New Hampshire Oregon Texas Washington None Lepus californicus Sylvilagus audubonii Sylvilagus bachmani Sylvilagus floridanus Sylvilagus nuttallii Kingdom Animalia animals Subkingdom Bilateria triploblasts Infrakingdom Deuterostomia Phylum Chordata chordates Subphylum Vertebrata vertebrates Infraphylum Gnathostomata Superclass Tetrapoda Class Mammalia mammals Subclass Theria Infraclass Eutheria Order Lagomorpha lagomorphs Rabbits Hares Pikas Family Leporidae Hares Rabbits Genus Lepus Hares Jackrabbits Jack Rabbits Species Lepus californicus Black-tailed Jack Rabbit Black-tailed Jackrabbit California Jackrabbit Gray-sided Jackrabbit Narrow-gauge Mule Jackass-hare Texas Jack Texan Hare Great Plains Jackrabbit TSN: 180115 Genus Sylvilagus Cottontails Species Sylvilagus audubonii Desert Cottontail Audubon's Cottontail TSN: 180122 Species Sylvilagus bachmani Brush Rabbit Riparian Brush Rabbit TSN: 180123 Species Sylvilagus floridanus Eastern Cottontail Florida cottontail TSN: 180124 Species Sylvilagus nuttallii Nuttall's cottontail Mountain Cottontail TSN: 180126 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Robert J Dusek USGS - MIDCONTINENT REGION Wildlife Biologist mailing and physical

National Wildlife Health Ctr,NWHC - Main Building

6006 Schroeder Rd.

Madison WI 53711 608-270-2403 rdusek@usgs.gov We greatly appreciate the efforts of the many agencies and staff, and volunteer organizations who submitted rabbits for diagnostic evaluation. Health and Biosecurity, CSIRO, Acton, ACT 2601, Australia Natural Science Research Laboratory, Museum of Texas Tech University, Lubbock, TX Texas Tech University, Lubbock, TX USGS National Wildlife Health Center, Madison, WI 2025 publication Virus Evolution Oxford University Press Jenckel, M., W-S Chang, E.A. Wright, R. Bradley, R.J. Dusek, H. Ip, R. Hall, I. Smith, T. Strive. Identification of novel hepaciviruses via pathogen profiling in North American lagomorphs. Virus Evolution. The data were extracted from the NWHC laboratory information management system and provided by State, Federal, and Tribal partners. No formal positional accuracy tests were conducted. The data were reviewed for appropriateness value ranges. Rabbit tissues were selected for inclusion based on obtaining a broad geographical distribution across the United States and rabbits that tested negative for rabbit hemorrhagic disease virus 2. n/a n/a Rabbits that tested positive for RHDV2 were not included. Rabbits included were selected based on obtaining as broad a geographical representation as possible within the limits of what was submitted to NWHC for diagnostic testing. 2025 Rabbit metagenomics specimen data.csv Comma Separated Value (CSV) file containing data. Producer Defined Sample number Unique identifier for a sample to be analyzed. Producer Defined Pooled tissues from multiple accessions to be analyzed together are indicated by the same sample number. Case-accession number Unique animal identifier assigned at time of submission. Producer Defined ‘Case’ identifies a unique rabbit or group of rabbits collected from the same approximate time and location. ‘Accession’ indicates an individual animal within a ‘case’. Species (Scientific) Scientific name of species as identified by submitter. Producer Defined ITIS Integrated Taxonomic Information System Species (common) Common name of species as identified by submitter. Producer Defined ITIS Integrated Taxonomic Information System State United States state of location (or equivalent). Reported by submitter. Producer Defined United States state of location (or equivalent). County County of location (or equivalent). Reported by submitter. Producer Defined County of location (or equivalent) Tissue Specific tissue(s) used for metagenomic analysis. Producer Defined Liver Liver sample Producer defined Liver/Spleen Pooled liver and spleen samples Producer defined Collection date Date individual animal was collected on as reported by submitter. Producer Defined Date "mm/dd/yyyy" U.S. Geological Survey - ScienceBase mailing address

Denver Federal Center

Building 810

Mail Stop 302

Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P13E4DAC None 20250314 Robert J Dusek USGS - MIDCONTINENT REGION Wildlife Biologist mailing and physical

National Wildlife Health Ctr,NWHC - Main Building

Madison WI 53711 608-270-2403 rdusek@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999