Kristin M. Romanok Kelly L. Smalling Paul M. Bradley Elliott P. Barnhart Margaret J. Eggers John T. Doyle Shannon M. Meppelink Molly L. Schreiner 20260126 Tabular digital data Denver, CO U.S. Geological Survey Romanok, K.M., Smalling, K.L., Bradley, P.M., Barnhart, E.P., Eggers, M.J., Doyle, J.T., Meppelink, S.M., and Schreiner, M.L., 2026, Concentration results for per- and polyfluoroalkyl substances (PFAS) analyzed in fish tissue, bed sediment, and biofilm samples, Montana, 2023: U.S. Geological Survey data release, https://doi.org/10.5066/P1DAMSMX. https://doi.org/10.5066/P1DMSMX Kelly L. Smalling Paul M. Bradley Kristin M. Romanok John T. Doyle Margaret J. Eggers Christine Martin Elliott P. Barnhart Picabo Binette Eric M. Castro Madisan Chavez Stephanie A. Ewing Stephanie E. Gordon Mathew W. Fields James L. Gray Ashley M. Groshong Chiachi Hwang Leslie K. Kanagy JoRee WClay LaFrance Keith A. Loftin Carrie Mae Long R. Blaine McCleskey Shannon M. Meppelink Crystal L. Richards Molly L. Schreiner Jonathan I. Shikany Mahelat Tesfamariam 2026 publication Environmental Science: Processes & Impacts vol. 28, issue 2 n/a Royal Society of Chemistry (RSC) ppg. 579-597 https://doi.org/10.1039/d5em00565e This data release contains the concentration results of per- and polyfluoroalkyl substances (PFAS) collected in fish tissue and whole blood, bed sediment and biofilm samples from water bodies in and surrounding the Little Bighorn River (LBHR) in Montana in July 2023. A total of 29 filets and whole blood samples were collected from 10 brown trout, 10 catfish, 6 rock bass, and 3 goldeye specimens collected by U.S. Geological Survey personnel and community volunteers in two locations along the LBHR. Bed sediment samples were collected from the top 2.54 centimeters in depositional zones in 12 sampling locations where surface-water-quality samples also were collected (Romanok and others, 2023). Biofilm scrapings were collected from rocks at five locations on the LBHR where bed-sediment and surface-water-quality samples were collected. Fish filet, whole blood, and biofilm samples were analyzed for 28 PFAS at RTI Laboratories, Inc., Livonia, Michigan, following EPA method 537.1 (liquid chromatography, tandem mass spectrometry). Bed-sediment samples were analyzed for 40 PFAS at SGS AXYS, Sidney, British Columbia, Canada, also using EPA method 537.1. References cited: Romanok, K.M., Bradley, P.M., Smalling, K.L., Doyle, J.T., Eggers, M.J., Gray, J.L., Hladik, M.L., Kanagy, L.K., McCleskey, R.B., Meppelink, S.M., Richards, C.L., and Roth, D.A., 2023, Target-chemical concentrations and microbiological results in surface water and tapwater, Montana, 2022-23 (ver. 2.0, December 2025): U.S. Geological Survey data release, https://doi.org/10.5066/P9R7MV6I. These data were collected in conjunction with a larger study conducted by the U.S. Geological Survey, Environmental Health Program, Drinking Water and Wastewater Infrastructure Project assessing water-quality concerns in tapwater and surface water along the Little Bighorn River. Tapwater and surface-water samples are reported in Romanok and others (2023). The aforementioned study analyzed samples for a broad suite of inorganic and organic contaminants, of which PFAS was included. To help assess the quality of the broader ecosystem, fish filet and whole blood samples were collected as well as bed sediment and biofilm samples to determine if PFAS is present in the ecosystem. References cited: Romanok, K.M., Bradley, P.M., Smalling, K.L., Doyle, J.T., Eggers, M.J., Gray, J.L., Hladik, M.L., Kanagy, L.K., McCleskey, R.B., Meppelink, S.M., Richards, C.L., and Roth, D.A., 2023, Target-chemical concentrations and microbiological results in surface water and tapwater, Montana, 2022-23 (ver. 2.0, December 2025): U.S. Geological Survey data release, https://doi.org/10.5066/P9R7MV6I. 20230723 20230726 ground condition Complete None planned -116.0600 -104.0400 49.0000 44.3500 ISO 19115 Topic Category biota geoscientificInformation environment health inlandWaters None surface water bed sediment fish tissue biofilm USGS Thesaurus water quality PFAS environmental health (human) surface water quality USGS Metadata Identifier USGS:67c8856bd34ea599a3b9ac74 Common Geographic Areas Montana United States None Hiodon alosoides Ambloplites rupestris Siluriformes Salmo trutta Kingdom Animalia animals Subkingdom Bilateria triploblasts Infrakingdom Deuterostomia Phylum Chordata chordates Subphylum Vertebrata vertebrates Infraphylum Gnathostomata Superclass Actinopterygii ray-finned fishes spiny rayed fishes Class Teleostei Superorder Osteoglossomorpha Order Osteoglossiformes bonytongues Suborder Notopteroidei Superfamily Hiodontoidea Family Hiodontidae mooneyes Genus Hiodon mooneyes Species Hiodon alosoides goldeye TSN: 161905 Superorder Acanthopterygii Order Perciformes perch-like fishes Suborder Percoidei Family Centrarchidae sunfishes Genus Ambloplites rock basses Species Ambloplites rupestris Rock Bass TSN: 168097 Superorder Ostariophysi Order Siluriformes catfishes TSN: 163992 Superorder Protacanthopterygii Order Salmoniformes salmons Family Salmonidae salmonids trouts and salmons salmons trouts Subfamily Salmoninae Genus Salmo Atlantic salmon Species Salmo trutta brown trout TSN: 161997 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata to understand appropriate use and data limitations. Kelly L. Smalling U.S. Geological Survey, New Jersey Water Science Center Research Hydrologist physical

3450 Princeton Pike, Suite 110

Lawrenceville New Jersey 08648 US 609-331-4850 ksmall@usgs.gov This work was funded by the U.S. Geological Survey, Ecosystems Mission Area, Environmental Health Program. Tables were generated in Microsoft Excel and should be imported as tab-delimited text (.txt) files to keep the integrity of values (no loss of leading or trailing zeros). Kelly L. Smalling Ashok D. Deshpande Vicki S. Blazer Bruce W. Dockum DeMond Timmons Beth L. Sharack Ronald J. Baker Jennifer Samson Timothy J. Reilly 2016 Publication Marine Pollution Bulletin vol. 107, issue 2 Amsterdam, Netherlands Elsevier BV Smalling, K.L., Deshpande, A.D., Blazer, V.S., Dockum, B.W., Timmons, D., Sharack, B.L., Baker, R.J., Samson, J., and Reilly, T.J., 2016, Young of the year bluefish (Pomatomus saltatrix) as a bioindicator of estuarine health: Establishing a new baseline for persistent organic pollutants after Hurricane Sandy for selected estuaries in New Jersey and New York: Marine Pollution Bulletin, v. 107, no. 2, accessed on February 19, 2025, at https://doi.org/10.1016/j.marpolbul.2016.03.019 https://doi.org/10.1016/j.marpolbul.2016.03.019 Vicki S. Blazer Heather L. Walsh Ryan P. Braham Cheyenne Smith 2018 Publication Journal of Visualized Experiments issue 139; e57946 Cambridge, MA MyJove Corporation Blazer, V.S., Walsh, H.L., Braham, R.P., and Smith, C., 2018, Necropsy-based wild fish health assessment: Journal of Visualized Experiments, no. 139, e57946, accessed on February 19, 2025, at https://doi.org/10.3791/57946 https://doi.org/10.3791/57946 Dean B. Radtke 2005 Version 1.1 Publication Techniques of Water-Resources Investigations 09-A8 Reston, VA U.S. Geological Survey Radtke, D.B., 2005, Nation field manual for the collection of water-quality data: Chapter A8, Bottom-material samples (ed.1.1): U.S. Geological Survey Techniques of Water-Resources Investigations, book 9; chap. A8, accessed November 19, 2025, at https://doi.org/10.3133/twri09A8. https://doi.org/10.3133/twri09A8 Ying Zhang Zhiqian Qv Jingwen Wang Yi Yang Xin Chen Jingzhen Wang Yanfeng Zhang Lingyan Zhu 2022 Publication Water Research vol. 215 Amsterdam, Netherlands Elsevier BV Zhang, Y., Qv, Z., Wang, J., Yang, Y., Chen, X., Wang, J., Zhang, Y., and Zhu, L., 2022, Natural biofilm as a potential integrative sample for evaluating the contamination and impacts of PFAS on aquatic ecosystems: Water Research, v. 215, 9 p., accessed on February 19, 2025, at https://doi.org/10.1016/j.watres.2022.118233 https://doi.org/10.1016/j.watres.2022.118233 J.A. Shoemaker D.R. Tettenhorst 2020 Version 2.0 Publication Cincinnati, OH U.S. Environmental Protection Agency Shoemaker, J.A., and Tettenhorst, D.R., 2020, Method 537.1 Determination of selected per- and polyfluorinated alkyl substances in drinking water by solid phase extraction and liquid chromatography/tandem mass spectrometry (LC/MS/MS), version 2.0: U.S. Environmental Protection Agency, Cincinnati, Ohio, accessed on February 19, 2025, at https://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=P10111J4.txt. https://nepis.epa.gov/Exe/ZyPURL.cgi?Dockey=P10111J4.txt Kristin M. Romanok Paul M. Bradley Kelly L. Smalling John T. Doyle Margaret J. Eggers James L. Gray Michelle L. Hladik Leslie K. Kanagy R. Blaine McCleskey Shannon M. Meppelink Crystal L. Richards David A. Roth 2023 Dataset Denver, CO U.S. Geological Survey Romanok, K.M., Bradley, P.M., Smalling, K.L., Doyle, J.T., Eggers, M.J., Gray, J.L., Hladik, M.L., Kanagy, L.K., McCleskey, R.B., Meppelink, S.M., Richards, C.L., and Roth, D.A., 2023, Target-chemical concentrations and microbiological results in surface water and tapwater, Montana, 2022-23 (ver. 2.0, December 2025): U.S. Geological Survey data release, https://doi.org/10.5066/P9R7MV6I https://doi.org/10.5066/P9R7MV6I Data included in these tables were quality assured through comparison with original data provided by the laboratories to be sure no transcription errors occurred, and evaluated against laboratory blanks and surrogate data, where available. Laboratory blank results are summarized in the text file MT2023_Table4_PFAS_QC_LabBlanks.txt included in this data release. Stable isotope recovery surrogates were used to evaluate method performance and are summarized in the text files MT2023_Table5a_PFAS_QC_Fish_Surrogates.txt, MT2023_Table5b_PFAS_QC_Sed_Surrogates.txt, and MT2023_Table5c_PFAS_QC_Biofilm_Surrogates.txt included in this data release. Laboratory matrix spikes also were evaluated and results are reported in the text files MT2023_Table6a_PFAS_QC_Fish_MatrixSpikes.txt, MT2023_Table6b_PFAS_QC_Sediment_MatrixSpikes.txt, and MT2023_Table6c_PFAS_QC_Biofilm_MatrixSpikes.txt in this data release. Fish filet and whole blood quality assurance (QA) summary: Results for two fish-filet samples (MT_LBHRCA14F and MT_LBHRCA15F) were not reported in the dataset because the spike recovery was near 0 percent indicating some issue during extraction and (or) analysis. Any PFAS result in fish filet or whole blood that had a corresponding surrogate outside the calibration range (20-150 percent) was coded as E, for estimated. Two PFAS (PFBA and PFPeA) were detected in associated laboratory blanks, but at concentrations an order of magnitude below what was reported in the environmental samples, therefore, no adjustments were made to the dataset. Sediment QA summary: PFOSA was detected in the associated laboratory blank (E 0.052 nanograms per gram [ng/g]), resulting in censoring all four detected values. Some values were not reported by the laboratory because of internal QA issues and are reported as an 'x' in the dataset. Biofilm QA summary: PFBA was detected in the associated laboratory blank, resulting in the censoring of all 5 detections; results are reported as an 'x'. Data are deemed to be accurate as reviewed for logical consistency. Dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record for additional details. No formal positional accuracy tests were conducted. No formal positional accuracy tests were conducted. Fish tissue and whole blood samples were collected and processed following procedures outlined in Blazer and others (2018) and Smalling and others (2016). A total of 29 filets and whole blood samples were collected from 10 brown trout, 10 catfish, 3 goldeye, and 6 rock bass. All samples were placed on ice at the time of collection and shipped to the U.S. Geological Survey (USGS) New Jersey Water Science Center (NJWSC) and frozen until they were shipped to the analyzing laboratories. The filet and whole blood analysis was conducted at RTI Laboratories, Inc., Livonia, Michigan. RTI laboratories prepared the samples for PFAS analysis by LC-MS/MS for compounds outlined in EPA method 537.1 (Shoemaker and Tennenhorst, 2020). Results are reported in MT2023_Table3a_PFAS_Fish_Results.txt in this data release. Sediment samples were collected using stainless steel spoons that were pre-cleaned and methanol rinsed. Samples were collected from depositional zones in the stream reach from the top 2.54 centimeters of sediment, as described in Radtke (2005). Sediment from approximately five different locations along the same side of the stream was collected and composited into clean wide-mouth, high density polyethylene (HDPE) jars from each stream reach. Samples were not sieved. Samples were placed on ice in the field then shipped cold to the USGS NJWSC by overnight delivery and frozen until shipped to SGS AXYS Laboratory, British Columbia, Canada. Samples were extracted to 4,000 microliters (uL) and 2 uL of extract was analyzed using LC-MS/MS. Mass (in grams) and percent moisture were measured and are reported along with concentration results in MT2023_Table3b_PFAS_Sediment_Results.txt in this data release. Biofilm samples were collected at 5 locations along the Little Bighorn River. At each location 3-4 rocks that were covered in biofilm were selected for sampling. Biofilm was scraped from all rocks at each sample location following procedures outlined in Zhang and others (2022), using stainless steel knives, and composited in plastic bags and frozen until analysis. Ethanol and flame sterilization were used to clean the scraping knife between sampling locations. Frozen samples were stored at the USGS NJWSC until shipped to RTI Laboratories, Inc., Livonia, Michigan. Samples were prepared for analysis by LC-MS/MS at the laboratory. Results are reported in MT2023_Table3c_PFAS_Biofilm_Results.txt in this data release. See the cross-reference section of this metadata for full citation information. Radtke, 2005 Shoemaker and Tennehorst, 2020 Zhang and others, 2022 Blazer and others, 2018 Smalling and others, 2016 2024 MT2023_Table1_SiteInformation.txt Table 1. Site and sample information for fish filet and whole blood, bed sediment, and biofilm samples collected in streams sampled in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number USGS_station_number U.S. Geological Survey station number U.S. Geological Survey U.S. Geological Survey station number, preceded by a "T" to maintain number integrity. USGS_station_name U.S. Geological Survey station name U.S. Geological Survey U.S. Geological Survey station name Sample_date_yyyymmdd Date of sample collection U.S. Geological Survey 20230723 20230726 Date, yyyy, year; mm, month; dd, day Sample_time_HHMM Time of sample collection U.S. Geological Survey 0700 1515 Time, HH, military-hour; MM, minute; --, not noted Sample_type Description of sample U.S. Geological Survey Fish filet Fish tissue filet sample U.S. Geological Survey Whole blood Fish whole blood sample U.S. Geological Survey Bed sediment Bed sediment sample U.S. Geological Survey Biofilm Biofilm sample U.S. Geological Survey Fish_specimen_number Identification number of fish specimen collected U.S. Geological Survey -- Not applicable U.S. Geological Survey 01 19 count Common_species_name Common name of fish. U.S. Geological Survey -- Not applicable U.S. Geological Survey See Taxonomy section of this metadata for additional species information. Fish_length_inches Length of fish measured in inches. U.S. Geological Survey -- Not applicable U.S. Geological Survey 5.5 15.5 inches MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt Table 2. List of per- and polyfluoroalkyl substances analyzed in fish filet, whole blood, bed sediment, and biofilm samples, Montana, 2023. U.S. Geological Survey Compound_name Per- and polyfluoroalkyl substance (PFAS) name U.S. Geological Survey Full name of PFAS Abbreviated_name Abbreviation of PFAS listed in column 1. U.S. Geological Survey PFAS abbreviation Sample_type_analyzed Sample medium in which PFAS was analyzed U.S. Geological Survey Bed sediment Bed sediment sample, analyzed at SGS AXYS, British Columbia, Canada. U.S. Geological Survey Fish filet Fish tissue filet sample, analyzed at RTI Laboratories, Inc., Livonia, Michigan. U.S. Geological Survey Whole blood Fish whole blood sample, analyzed at RTI Laboratories, Inc., Livonia, Michigan. U.S. Geological Survey Biofilm Biofilm sample, analyzed at RTI Laboratories, Inc., Livonia, Michigan. U.S. Geological Survey MT2023_Table3a_PFAS_Fish_Results.txt Table 3a. Concentration results for fish filet (in micrograms per kilogram, wet weight [ug/kg, ww]) and whole blood (in nanograms per milliliter [ng/mL]) samples collected from two streams in Montana, 2023. U.S Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number USGS_station_number U.S. Geological Survey station number U.S. Geological Survey USGS station number, preceded by a "T" to maintain number integrity. USGS_station_name U.S. Geological Survey station name U.S. Geological Survey U.S. Geological Survey station name Fish_specimen_number Identification number of fish specimen collected U.S. Geological Survey -- Not applicable U.S. Geological Survey 01 19 count Sample_type_with_units Description of sample U.S. Geological Survey Fish filet (ug/kg) Fish tissue filet reported in micrograms per kilogram. U.S. Geological Survey Whole blood (ng/mL) Fish whole blood sample reported in nanograms per milliliter. U.S. Geological Survey Common_species_name Common name of fish U.S. Geological Survey See Taxonomy section of this metadata for additional species information. Columns 7-34 Concentration results in fish filet and whole blood samples, reported in ug/kg and ng/mL wet weight, respectively, corresponding to column headers: PFOS; PFBA; PFUdA; PFDA; PFNA; PFTrDA; PFDoA; PFPeA; PFDS; PFHxS; PFTA; PFOA; 6:2_FTS; NMeFOSAA; PFHxA; 11Cl_PF3OYUdS; 8:2_FTS; 4:2_FTS; 9Cl_PF3ONS; ADONA; FOSA; GenX; NEtFOSAA; PFBS; PFHpS; PFHpA; PFPeS; PFNS U.S. Geological Survey Concentration results in ug/kg and ng/mL (equivalent units). Results for two fish filet samples (MT_LBHRCA14F and MT_LBHRCA15F) were not reported in the dataset because the spike recoveries were near 0 percent indicating some issue during extraction and (or) analysis. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. [Less than sign, value not detected above listed reporting level (non-detect); E, estimated.] MT2023_Table3b_PFAS_Sediment_Results.txt Table 3b. Concentration results, reported in nanograms per gram (ng/g) dry weight (dw), for per- and polyfluoroalkyl substances (PFAS) analyzed in bed sediment samples collected from streams in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number USGS_station_number U.S. Geological Survey station number U.S. Geological Survey USGS station number, preceded by a "T" to maintain number integrity. USGS_station_name U.S. Geological Survey station name U.S. Geological Survey U.S. Geological Survey station name Sample_date_yyyymmdd Date of sample collection U.S. Geological Survey 20230724 20230726 Date, yyyy, year; mm, month; dd, day Sample_time_HHMM Time of sample collection U.S. Geological Survey 0700 1515 Time; HH, military-hour; MM, minute Mass_gram_dry Mass of sample reported in grams, dry weight U.S. Geological Survey 2.78 5.77 grams, dry weight Moisture_percent Percent of moisture content in sample U.S. Geological Survey 20.8 62.4 percent Columns 8-47 Concentration results in sediment samples, reported in nanograms per gram, dry weight, corresponding to headers: PFBA; PFPeA; PFHxA; PFHpA; PFOA; PFNA; PFDA; PFUnA; PFDoA; PFTrDA; PFTeDA; PFBS; PFPeS; PFHxS; PFHpS; PFOS; PFNS; PFDS; PFDoS; 4:2_FTS; 6:2_FTS; 8:2_FTS; PFOSA; N_MeFOSA; N_EtFOSA; MeFOSAA; EtFOSAA; N_MeFOSE; N_EtFOSE; HFPO_DA_(Gen X); ADONA; 9Cl_PF3ONS; 11Cl_PF3OUdS; 3:3_FTCA; 5:3_FTCA; 7:3_FTCA; PFEESA; PFMPA; PFMBA; NFDHA U.S. Geological Survey PFOSA was detected in the laboratory blank (E 0.052 ng/g), resulting in censoring all four detected values. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. [ng/g, nanograms per gram; less than sign, less than reporting limit (not detected); x, value not reported because of the laboratory blank detection; E, estimated value.] MT2023_Table3c_PFAS_Biofilm_Results.txt Table3c. Concentration results, reported in nanograms per gram (ng/g), wet weight (ww), for per- and polyfluoroalkyl substances (PFAS) analyzed in biofilm samples collected streams in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number USGS_station_number U.S. Geological Survey station number U.S. Geological Survey USGS station number, preceded by a "T" to maintain number integrity. USGS_station_name U.S. Geological Survey station name U.S. Geological Survey U.S. Geological Survey station name Sample_type Description of sample U.S. Geological Survey Biofilm sample Sample_date_yyyymmdd Date of sample collection U.S. Geological Survey 20230725 20230726 Date, yyyy, year; mm, month; dd, day Sample_time_HHMM Time of sample collection U.S. Geological Survey 0730 1315 Time; HH, military-hour; MM, minute Columns 7-34 Concentration results in biofilm, reported in nanograms per gram, wet weight, corresponding to headers: PFBA; PFPeA; PFHxA; PFHpA; PFOA; PFNA; PFDA; PFUdA; PFDoA; PFTrDA; PFTA; PFBS; PFPeS; PFHxS; PFHpS; PFOS; PFNS; PFDS; 4:2_FTS; 6:2_FTS; 8:2_FTS; FOSA; N_MeFOSAA; N_EtFOSSA; GenX; ADONA; 9Cl_PF3ONS; 11Cl_PF3OYUdS U.S. Geological Survey PFBA was detected in the laboratory blank, resulting in the censoring of all 5 detections. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. [ng_per_g_ww, nanograms per gram, wet weight; E, estimated; x, censored because of the detection in laboratory blank sample.] MT2023_Table4_PFAS_QC_LabBlanks.txt Table 4. Detected quality-control laboratory blank results for batches corresponding to fish filet, bed sediment, and biofilm samples, reported in units listed, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Description of laboratory blank associated sample type: fish filet, bed sediment, or biofilm. Abbreviated_name List of detected per- and polyfluoroalkyl substance (abbreviation). U.S. Geological Survey See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. Remark_and_result Concentration result and remark of detected PFAS in column 3. U.S. Geological Survey Concentration in units listed in column 5. Units_of_measurement Units of measurement for concentrations in column 4. U.S. Geological Survey ng/g dw Nanograms per gram dry weight U.S. Geological Survey ng/L Nanograms per liter U.S. Geological Survey Analyzing_laboratory Laboratory performing analysis U.S. Geological Survey RTI RTI Laboratories, Inc., Livonia, Michigan U.S. Geological Survey SGS SGS AXYS, British Columbia, Canada U.S. Geological Survey MT2023_Table5a_QC_PFAS_Fish_Surrogates.txt Table 5a. Surrogate results, in percent recovery, analyzed in conjunction with fish-filet, whole-blood, and associated quality-control samples, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Fish filet Fish tissue filet sample U.S. Geological Survey Whole blood Fish whole blood sample U.S. Geological Survey Laboratory blank Laboratory quality control blank used to evaluate possible laboratory contamination. U.S. Geological Survey Laboratory control sample Laboratory Control Sample (LCS); prepared by adding a known amount of target analytes to a specified amount of clean matrix and prepared with the batch of samples, used to calculate accuracy (percent recovery). U.S. Geological Survey Laboratory control duplicate sample A duplicate LCS sample, used to calculate both accuracy (percent recovery) and precision (relative percent difference). U.S. Geological Survey Columns 3-26 Surrogate results, in percent recovery, analyzed in conjunction with fish tissue filet, whole blood, and quality assurance samples, corresponding to column headers: 13C2_4:2FTS; 13C2_6:2FTS; 13C2_8:2FTS; 13C2_PFTeDA; 13C3_PFBS; 13C3_PFHxS; 13C8_PFOSA; D3_NMeFOSAA; D5_NEtFOSAA; M2PFDoA; M3HFPODA; M4PFHpA; M5PFHxA; M5PFPeA; M6PFDA; M7PFUnA; M8PFOA; M8PFOS; M9PFNA; MFPBA; D3_NMeFOSA; D5_NEtFOSA; D7_NMeFOSE; D9_NEtFOSE U.S. Geological Survey Quality-control surrogate results in percent recovery results. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details; --, no data. MT2023_Table5b_PFAS_QC_Sed_Surrogates.txt Table 5b. Surrogate results, in percent recovery, analyzed in conjunction with bed sediment and associated quality-control samples, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Bed sediment Bed sediment sample U.S. Geological Survey Laboratory blank Quality control laboratory blank used to evaluate possible laboratory contamination. U.S. Geological Survey Laboratory control sample Quality control laboratory reference or spike sample U.S. Geological Survey Columns 3-26 Surrogate results, in percent recovery, analyzed in conjunction with bed sediment and quality-control samples, corresponding to headers: 13C4_PFBA; 13C5_PFPeA; 13C5_PFHxA; 13C4_PFHpA; 13C8_PFOA; 13C9_PFNA; 13C6_PFDA; 13C7_PFUnA; 13C2_PFDoA; 13C2_PFTeDA; 13C3_PFBS; 13C3_PFHxS; 13C8_PFOS; 13C2_4:2_FTS; 13C2_6:2_FTS; 13C2_8:2_FTS; 13C8_PFOSA; D3_N_MeFOSA; D5_N_EtFOSA; D3_MeFOSAA; D5_EtFOSAA; d7_NMe_FOSE; d9_NEt_FOSE; 13C3_HFPO_DA_(GenX) U.S. Geological Survey Quality-control surrogate results in percent recovery results. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. MT2023_Table5c_PFAS_QC_Biofilm_Surrogates.txt Table 5c. Surrogate results, in percent recovery, analyzed in conjunction with biofilm and associated quality-control samples, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Biofilm Biofilm samples U.S. Geological Survey Laboratory blank Quality assurance laboratory blank used to evaluate possible laboratory contamination. U.S. Geological Survey Laboratory control sample Laboratory Control Sample (LCS); prepared by adding a known amount of target analytes to a specified amount of clean matrix and prepared with the batch of samples, used to calculate accuracy (percent recovery). U.S. Geological Survey Laboratory control duplicate sample A duplicate LCS sample, used to calculate both accuracy (percent recovery) and precision (percent recovery). U.S. Geological Survey Columns 3-26 Surrogate results, in percent recovery, analyzed in conjunction with biofilm and quality-assurance samples, corresponding to headers: 13C2_4:2FTS; 13C2_6:2FTS; 13C2_8:2FTS; 13C2_PFTeDA; 13C3_PFBS; 13C3_PFHxS; 13C8_PFOSA; D3_NMeFOSA; D3_NMeFOSAA; D5_NEtFOSA; D5_NEtFOSAA; D7_NMeFOSE; D9_NEtFOSE; M2PFDoA; M3HFPODA; M4PFHpA; M5PFHxA; M5PFPeA; M6PFDA; M7PFUnA; M8PFOA; M8PFOS; M9PFNA; MFPBA U.S. Geological Survey Quality-control surrogate results in percent recovery results. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details; --, no data. MT2023_Table6a_PFAS_QC_Fish_MatrixSpikes.txt Table 6a. Laboratory quality-control matrix spike results, in percent recovery, analyzed in conjunction with fish filet, and whole blood samples, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Matrix spike Prepared by adding a known amount of target analytes to a specified amount of matrix sample for which an independent estimate of target analyte concentration is available, used to calculate accuracy (percent recovery). U.S. Geological Survey Matrix spike duplicate A duplicate matrix spike sample, used to calculate both accuracy (percent recovery) and precision (relative percent difference). U.S. Geological Survey Columns 3-30 Matrix spike results, in percent recovery, analyzed in conjunction with fish filet, and whole blood samples, corresponding to headers: PFBA; PFPeA; PFHxA; PFHpA; PFOA; PFNA; PFDA; PFUdA; PFDoA; PFTrDA; PFTA; PFBS; PFPeS; PFHxS; PFHpS; PFOS; PFNS; PFDS; 4:2_FTS; 6:2_FTS; 8:2_FTS; FOSA; NMeFOSAA; NEtFOSSA; GenX; ADONA; 9Cl_PF3ONS; 11Cl_PF3OYUdS U.S. Geological Survey Quality control matrix spike results in percent recovery. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. MT2023_Table6b_PFAS_QC_Sed_MatrixSpikes.txt Table 6b. Laboratory quality-control matrix spike results, in percent recovery, analyzed in conjunction with bed sediment samples, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Matrix spike Quality assurance matrix spike U.S. Geological Survey Columns 3-42 Matrix spike results, in percent recovery, analyzed in conjunction with bed sediment samples, corresponding to headers: PFBA; PFPeA; PFHxA; PFHpA; PFOA; PFNA; PFDA; PFUnA; PFDoA; PFTrDA; PFTeDA; PFBS; PFPeS; PFHxS; PFHpS; PFOS; PFNS; PFDS; PFDoS; 4:2_FTS; 6:2_FTS; 8:2_FTS; PFOSA; N_MeFOSA; N_EtFOSA; MeFOSAA; EtFOSSA; N_MeFOSE; N_EtFOSE; HFPO-DA_(GenX); ADONA; 9Cl_PF3ONS; 11Cl_PF3OUdS; 3:3_FTCA; 5:3_FTCA; 7:3_FTCA; PFEESA; PFMPA; PFMBA; NFDHA U.S. Geological Survey Quality-control matrix spike results in percent recovery. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. MT2023_Table6c_PFAS_QC_MatrixSpikes.txt Table 6c. Laboratory quality-control matrix spike results, in percent recovery, analyzed in conjunction with biofilm samples, in Montana, 2023. U.S. Geological Survey Sample_ID Sample identification number U.S. Geological Survey Sample identification number Sample_type Description of sample U.S. Geological Survey Matrix spike Prepared by adding a known amount of target analytes to a specified amount of matrix sample for which an independent estimate of target analyte concentration is available, used to calculate accuracy (percent recovery). U.S. Geological Survey Matrix spike duplicate A duplicate matrix spike sample, used to calculate both accuracy (percent recovery) and precision (relative percent difference). U.S. Geological Survey Columns 3-30 Matrix spike results, in percent recovery, analyzed in conjunction with biofilm samples, corresponding to headers: PFBA; PFPeA; PFHxA; PFHpA; PFOA; PFNA; PFDA; PFUdA; PFDoA; PFTrDA; PFTA; PFBS; PFPeS; PFHxS; PFHpS; PFOS; PFNS; PFDS; 4:2_FTS; 6:2_FTS; 8:2_FTS; FOSA; NMeFOSAA; NEtFOSSA; HFPO_DA_(GEN X); ADONA; 9Cl_PF3ONS; 11Cl_PF3OYUdS U.S. Geological Survey Quality-control matrix spike results in percent recovery. See MT2023_Table2_PFAS_Tis_Sed_Biofilm_MethodInfo.txt for compound details. GS ScienceBase U.S. Geological Survey mailing address

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