Nicholas S. Barnes James R. Rodgers Christopher P. Bonvillain Matthew J. Catalano William R. Budnick Annie Allert James A. Stoeckel Rachel G. Nelson 20250725 tabular digital data https://doi.org/10.5066/P1JJEGG9 This study used artificial burrowing chambers to investigate the effects of groundwater drainage rates and soil type on burrow initiation and morphology of red swamp crayfish (Procambarus clarkii). The relationship between caloric density of crayfish and burrowing was also examined. The objectives of this study were to (1) determine the effect of ground water drainage rates on burrowing, (2) determine the effect of soil composition on burrowing, and (3) test for differences in burrowing behaviors between males and females. These datasets include data collected from groundwater drainage rate trials and soil composition trials. These data were collected to highlight the influences of groundwater, soil, and energy stores on RSC burrowing activity and suggest potential strategies for managing invasive populations by manipulating water levels and habitat conditions. 20230328 20240129 ground condition Complete None planned Auburn University EW Shell Fisheries Station Laboratory -180.0000 180.0000 90.0000 -90.0000 ISO 19115 Topic Category boundaries None Procambarus clarkii burrowing behavior USGS Thesaurus invasive species animal behavior aquaculture bioenergetics USGS Metadata Identifier USGS:67925ea7d34e88f5864c4862 None Procambarus clarkii Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Crustacea Superclass Altocrustacea Class Malacostraca Subclass Eumalacostraca Superorder Eucarida Order Decapoda Suborder Pleocyemata Infraorder Astacidea Superfamily Astacoidea Family Cambaridae Genus Procambarus Species Procambarus clarkii TSN: 97491 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Rachel G Nelson U.S. Geological Survey, MIDCONTINENT REGION Biologist mailing

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La Crosse WI 54603 US 608-781-6377 rgnelson@usgs.gov Auburn University, Michigan DNR All instruments used for data collection were calibrated daily. Data values fall into expected ranges and datasets have been reviewed for duplication/omission. These datasets represent laboratory data collected the Auburn University EW Shell Fisheries Station. NA NA Groundwater Drainage Rate Each burrowing chamber was filled with soil and ground water raised to the surface via the internal standpipe for 24 hours to saturate the soil. RSC were trapped from a nearby pond with a high population of RSC, selecting males with 45-55 mm carapace lengths, and transported directly into the burrowing chamber lab. After 24 h of soil saturation, a single male RSC with a carapace length ranging from 45-55 mm was added to each acclimation chamber above the burrowing chamber and the groundwater immediately drained to the prescribed level associated with a particular treatment. Each chamber was assigned to one of three groundwater drainage treatments: 4 cm/day for 5 days, 8 cm/day for 3 days, or 24 cm/day for 1 day. Each trial contained nine burrowing chambers, with nine RSC being randomly assigned to one of the three treatments. After each trial, all chambers were broken down, had soil replaced, and set up again in the same manner, saturating the soil 24 hours prior to the introduction of the next RSC. The nine burrowing chamber trials were repeated four times, with12 total RSC assigned to each treatment. Regardless of treatment, all crayfish were allowed to burrow for a total of 5 days. Study initiation time was recorded after all crayfish were placed in chambers and water levels were reduced. At that time on each subsequent experimental day, a shade panel was lifted from the front of each chamber and burrow depth was recorded for each crayfish as the linear distance from the soil surface to the bottom of the burrow. 20230328 Soil Composition Burrowing chambers were modified to house two crayfish in each chamber for the soil composition experiment chamber. The soil used to fill burrowing chambers was high clay content (36.7% fine particles, Stoeckel et at. 2011) collected from the Auburn University EW Shell Fisheries Station where RSC frequently create natural burrows. This soil was mixed with increasing amounts of Quick-Crete Play Sand to create 10 different ambient soil:sand ratios: 100:0, 90:10, 80:20, 70:30, 60:40, 50:50, 40:60, 30:70, 20:80, and 10:90. Both sides of a given burrowing chamber were filled with the same soil:sand mixture. Red Swamp Crayfish for this study were collected on site from the E.W. Shell Fisheries Station at Auburn University and all measured between 45-55mm total carapace length. One male and one female were randomly assigned to one of two surface tanks on each burrowing chamber (one crayfish per surface tank). Based on the previous groundwater drainage experiment, we used a drainage rate of 4 cm/day in all soil types to provide favorable conditions for burrowing. After drawing down the groundwater for five days, tracings of the final burrow made by each crayfish were made by shining a light through the back plexiglass and placing a sheet of tracing paper over the front plexiglass. 20240125 Energetics The energetic health of each crayfish was assessed to determine whether non-burrowing crayfish had a lower energy density than burrowers. Bomb calorimetry was used to measure energetic content of each individual following the traditional approach of Glover et al. (2010). After the end of the soil composition trials, crayfish were euthanized via freezing and stored at -80℃. Within 60 days of collection all crayfish were thawed and then dried at 100 ℃ for 12 hours. After drying, the entire crayfish was ground into a fine powder using an analytical mill (IKA A11 basic analytical mill, 2635 Northchase Parkway SE Wilmington, NC 28405 USA) then pressed into a pellet weighing 0.1-0.2 grams. Each pellet was then ignited in a Parr 6725 semimicro calorimeter. Caloric values obtained for two pellets per crayfish were then averaged to obtain a caloric density for that individual (cal/g dry mass). 20240125 gw_exp.csv Comma Separated Value (CSV) file containing data. Producer Defined trmt Groundwater drainage treatment assigned to a test chamber. Producer Defined 4 4 cm of groundwater in a test chamber was drained daily for five consecutive days. Producer defined 8 8 cm of groundwater in a test chamber was drained daily for three consecutive days. Producer defined 24 24 cm of groundwater in a test chamber was drained for one day. Producer defined id ID given to individual crayfish. Producer Defined ID number in format "trial number - chamber number" day Day of experimental trial. Producer Defined 1 5 days depth_bur Depth in centimeters of each crayfish burrow measured from the soil surface to the bottom of the burrow. Producer Defined 0 20 cm mean Average depth burrowed in centimeters. Producer Defined NA No Data Producer defined 3.666666667 16.25 cm se Standard error - standard deviation of the sample distribution. Producer Defined NA No Data Producer defined 0.333333333 1.81794506 cm soil_type_exp.csv Comma Separated Value (CSV) file containing data. Producer Defined id ID number assigned to an individual crayfish. Producer Defined 1 20 none cl Carapace length in millimeters measured from the tip of the rostrum to the posterior edge of the carapace. Producer Defined 45 50 mm wt_pre Weight in grams of crayfish pretrial. Producer Defined 22 36 g wt_post Weight in grams of crayfish post-trial. Producer Defined 21 34 g chamber_id ID of test chamber used in trial. Producer Defined Test chambers were divided into two halves for soil composition experiments. Each side of the test chamber was given a label "a" or "b". sex Sex of crayfish. Producer Defined 0 male Producer defined 1 female Producer defined trmt % sand in soil:sand mixture. Ambient (AMB) soil didn't have any added sand. Producer Defined 40 40% sand Producer defined 70 70% sand Producer defined 20 20% sand Producer defined AMB Ambient (no sand added) Producer defined 50 50% sand Producer defined 30 30% sand Producer defined 10 10% sand Producer defined 80 80% sand Producer defined 60 60% sand Producer defined 90 90% sand Producer defined day1 Describes crayfish activity on day 1 of trial. Producer Defined Followed water line Burrowed to water line Producer defined No activity Crayfish did not show signs of movement or burrowing activity Producer defined No burrowing Crayfish did not burrow Producer defined day2 Describes crayfish activity on day 2 of trial. Producer Defined Followed water line Burrowed to water line Producer defined No activity Crayfish did not show signs of movement or burrowing activity Producer defined No burrowing Crayfish did not burrow Producer defined day3 Describes crayfish activity on day 3 of trial. Producer Defined Followed water line Burrowed to water line Producer defined No activity Crayfish did not show signs of movement or burrowing activity Producer defined No burrowing Crayfish did not burrow Producer defined day4 Describes crayfish activity on day 4 of trial. Producer Defined Followed water line Burrowed to water line Producer defined No activity Crayfish did not show signs of movement or burrowing activity Producer defined No burrowing Crayfish did not burrow Producer defined day5 Describes crayfish activity on day 5 of trial. Producer Defined Followed water line Burrowed to water line Producer defined No activity Crayfish did not show signs of movement or burrowing activity Producer defined No burrowing Crayfish did not burrow Producer defined notes Notes on crayfish burrowing activity. Producer Defined Notes on crayfish burrowing activity final_depth Final depth in centimeters of crayfish burrow. Producer Defined 0 24 cm final_area Final area in cm^2 of crayfish burrow. Producer Defined NA Not applicable Producer defined 9.851 25.014 cm^2 final_perim Final perimeter in centimeters of crayfish burrow. Producer Defined NA Not applicable Producer defined 16.662 34.522 cm final_cal_density Final caloric density of crayfish in cal/gDW. Producer Defined NA Not applicable Producer defined 1737.0 2961.6 cal/gDW GS ScienceBase U.S. Geological Survey mailing address

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Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P1JJEGG9 None 20250725 Rachel G Nelson U.S. Geological Survey, MIDCONTINENT REGION Biologist mailing

2630 Fanta Reed Road

La Crosse WI 54603 US 608-781-6377 rgnelson@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999