Robert Peck Dan Mikros Ellen Dunkle Kylle Roy 20250422 tabular digital data https://doi.org/10.5066/P14EOVKX Robert W. Peck Dan Mikros Ellen J. Dunkle Kelly Jaenecke Kylle Roy 20250811 publication Agricultural and Forest Entomology vol. 28, issue 1 n/a Wiley ppg. 49-60 https://doi.org/10.1111/afe.70007 This data release includes metadata and tabular data that documents the response of ohia (Metrosideros polymorpha) and ambrosia beetles (Curculionidae, Xyleborini) following the intentional inoculation of healthy ohia to Ceratocystis lukuohia, one of two fungal pathogens that causes Rapid Ohia Death (ROD). Ohia were inoculated with the ROD pathogen at three sites differing in elevation on the Island of Hawaii during January and June 2019. Inoculated and control trees were monitored for changes in foliage condition, beetle attack rates, beetle species composition, and the viability of the fungi within the tree through August 2021. These data are represented in the following seven files: 1) descriptions of trees, 2) tree conditions and the presence of beetle galleries, 3) beetles collected in boring dust traps (BDTs), 4) viability of fungi in beetle frass, 5) beetles collected on sticky traps, 6) descriptions of felled trees, and 7) beetles emerging from felled trees. Temporal interactions among the fungal pathogens that causes Rapid Ohia Death (ROD) and the ambrosia beetles that are involved in the spread of the disease are poorly understood. Data were collected to document the response of ohia (Metrosideros polymorpha), ambrosia beetles and pathogenic fungi following the intentional inoculation of healthy ohia with Ceratocystis lukuohia, the fungal pathogen that causes Ceratocystis wilt, the more damaging of the two fungi that cause ROD. Data from this study aim to provide managers with a better understanding of when beetles attack trees, how long beetles continue their attack, and how long viable fungal propagules may be released from an infected tree. 20190110 20220318 observed Complete None planned East side, Island of Hawaiʻi -155.3838 -154.9525 19.7525 19.4044 ISO 19115 Topic Category farming None Rapid Ohia Death fungal disease Ceratocystis Ambrosia beetles USGS Metadata Identifier USGS:677dd8dfd34e009b43365a4c None Island of Hawaii None Ceratocystis Metrosideros polymorpha Xyleborus ferrugineus Xyleborus simillimus Xyleborus perforans Xyleborus affinis Xyleborinus saxesenii Ceratocystis lukuohia Domain Eukaryota Kingdom Fungi Subkingdom Dikarya Division Ascomycota Subdivision Pezizomycotina Class Sordariomycetes Subclass Sordariomycetidae Order Ophiostomatales Family Ophiostomataceae Genus Ceratocystis TSN: 181740 Kingdom Plantae Subkingdom Viridiplantae Infrakingdom Streptophyta Superdivision Embryophyta Division Tracheophyta Subdivision Spermatophytina Class Magnoliopsida Superorder Rosanae Order Myrtales Family Myrtaceae Genus Metrosideros Species Metrosideros polymorpha TSN: 27259 Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda Class Insecta Subclass Pterygota Infraclass Neoptera Superorder Holometabola Order Coleoptera Suborder Polyphaga Infraorder Cucujiformia Superfamily Curculionoidea Family Curculionidae Subfamily Scolytinae Genus Xyleborus Species Xyleborus ferrugineus TSN: 188145 Species Xyleborus simillimus TSN: 188162 Species Xyleborus perforans TSN: 188158 Species Xyleborus affinis TSN: 188140 Genus Xyleborinus Species Xyleborinus saxeseni None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Robert W Peck Hawaii Cooperative Studies Unit, University of Hawaii at Hilo Research Specialist physical

Bldg 344 Chain of Craters Road

Hawaii National Park HI 96718 USA 808-210-6141 808-967-8568 bwpeck@usgs.gov We thank Theo Black and Josie Tupu for help in the field. We also thank Dustin Swan of the Big Island Invasive Species Committee for felling and sectioning trees. J.B. Friday and Susan Miyasaka generously provided laboratory space at the University of Hawaii at Manoa Komohana Research and Extension Center. Hawaii Department of Land and Natural Resources invertebrate collection and state forest access permits were issued by Cynthia King and Steve Bergfeld, respectively. We thank Craig Blaisdell for his continued support of ROD studies and access to the Keaukaha Military Reservation. This study was supported by U.S.D.A. Forest Service, Hawaii Division of Forestry and Wildlife, and the U.S. Geological Survey Biological Threats and Invasive Species Program. Windows 10, Excel 365 ProPlus Accuracy has been addressed separately for each of the seven datasets. All data are logical, consistent, and fall within expected ranges. All data were checked for duplications and omissions. Dataset is complete and represent results obtained at the three study sites on the Island of Hawaii during 10 January 2019 - 18 March 2022. NA NA Seven separate data files were created to document how affected trees, beetles and fungi respond to ohia inoculated with Ceratocystis lukuohia. These data are represented in the following seven files: 1) tree descriptions, 2) tree response, 3) beetles collected in boring dust traps (BDTs), 4) frass viability, 5) beetles collected on sticky cards, 6) characteristics of felled trees, and 7) beetles reared from felled trees. 20190110 Robert Peck physical

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Hawaii National Park HI 96718 808-210-6141 U.S. Geological Survey GS ScienceBase mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes and does not imply endorsement by the U.S. Government. Digital Data https://doi.org/10.5066/P14EOVKX None 20260224 U.S. Geological Survey - PIERC Data Steward physical

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Hawaii National Park HI 96718 USA 808-210-6141 pierc-datasteward@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999