Scott, Laura C. (ORCID: 0000-0003-0303-5340) Ahlstrom, Christina A. (ORCID: 0000-0001-5414-8076) Woksepp, Hanna (ORCID: 0000-0002-1707-2655) Bonnedahl, Jonas (ORCID: 0000-0002-3182-389X) McKeeman, Cherie M. (ORCID: 0000-0001-9868-2502) Miller, Mark E. (ORCID: 0000-0002-4691-7469) Shirokauer, Dave Ramey, Andrew M. (ORCID: 0000-0002-3601-8400) 20250421 tabular digital data Anchorage, Alaska U.S. Geological Survey, Alaska Science Center Suggested Citation: Scott, L.C., Ahlstrom, C.A., Woksepp, H., Bonnedahl, J., McKeeman, C.M., Miller, M.E., Shirokauer, D., Ramey, A.M., 2025, Data for validation and application of standardized quantitative PCR assay for the assessment of antimicrobial resistance genes in surface water: U.S. Geological Survey data release, https://doi.org/10.5066/P14G9MJW https://doi.org/10.5066/P14G9MJW This data set describes results for two laboratory experiments to validate a quantitative PCR assay for the assessment of antimicrobial resistance genes in surface waters. Data are also provided for the application of the assay surface water samples collected from three national parks in Alaska. Data were collected to develop a high throughput quantitative method for measuring antimicrobial resistance genes in water samples. Data can be used for epidemiological estimations of antimicrobial resistance genes in the presented samples. 20230601 20230731 observed Complete None planned State of Alaska -180.0000 -129.0000 72.0000 50.0000 USGS Metadata Identifier USGS:672d21f1d34ea9e45088e566 ISO 19115 Topic Category Biota Environment Health NASA GCMD Earth Science Keyword Thesaurus Bacteria/archaea Birds Freshwater ecosystems Aquatic ecosystems Ecological dynamics Public health Environmental health factors USGS CSA Biocomplexity Thesaurus Disease reservoirs Disease detection Disease spread Disease control Antimicrobial activity USGS Thesaurus Health and disease Bacteria Environmental DNA DNA sequencing NIH MeSH Thesaurus Drug resistance, microbial None Surface water Antimicrobial resistance USGS Geographic Names Information System (GNIS) Alaska USGS Protected Areas Database of the United States (PAD-US) Denali National Park and Preserve Kenai Fjords National Park Wrangell-St. Elias National Park and Preserve No access constraints. No use constraints. These data are marked with a Creative Common CC0 1.0 Universal License and are in the public domain. It is requested that this USGS data release be cited for any subsequent publications that reference or utilize these data. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov Scott, L.C. Ahlstrom, C.A. Woksepp, H. Bonnedahl, J. McHeeman, C.M. Miller, M.E. Shirokauer, D. Ramey, A.M. Unknown journal article Scientific Reports TBA online Springer Nature Scott, L.C., Ahlstrom, C.A., Woksepp, H., Bonnedahl, J., McKeeman, C.M., Miller, M.E., Shirokauer, D., Ramey, A.M., TBA. Validation and application of a standardized quantitative PCR assay for the assessment of antimicrobial resistance genes in surface water. Scientific Reports. TBA https://doi.org/10.1186/TBA Quantitative PCR (qPCR) markers were checked using NCBI Blast and the Comprehensive Antibiotic Resistance Database to confirm primers amplified the correct target. Data have been checked for completeness. Attribute values fall within expected ranges. Null values are described in the Entities and Attributes section. This dataset includes information for two laboratory studies to validate an assay for field use. These data do not represent natural phenomenon, as they were generated for experimental purposes. This dataset also includes information for a field study. Associated data represent values for only the surface waters tested at the date and time of collection. Quantitative PCR (qPCR): Each qPCR reaction was done in 20µl volumes with the following inclusions: 10µl of 2x PowerUp SYBR Mastermix (Applied Biosystems), 6µl of nanopure water, 2µl of template DNA, and 1µl of each forward and reverse primer. Final primer concentrations were 0.5µM and are listed for each target in Supplementary Table 1 of the associated publication. Thermal cycling conditions for the qPCR reaction were as follows: uracil-DNA glycosylase deactivation at 50℃ for 2 minutes, polymerase activation at 95℃ for 2 minutes, 40 cycles of 95℃ for 15 seconds followed by 60℃ for 30 seconds, and a melt curve analysis of 95℃ for 1 minute, 60℃ for 5 minutes, and gradual increase of temperature from 60℃ to 95℃ at 0.1℃/second. For every sample, every target was run in duplicate, and every plate included duplicate non-template control wells. The limit of detection for each target was designated as the lowest concentration that was detected in every standard curve replicate. Unknown Sampling locations are described without coordinates. Borough and census area names are used to describe general sampling locations. Point anitmicrobialResistance_surfaceWater_qPCR_bacterial_Isolates.csv Results of quantitative PCR (qPCR) analysis for antimicrobial resistance gene targets on bacterial isolates. Presented in a Comma Separated Value (CSV) formatted table. Author defined GeneID The GeneID consists of the gene name that is tested, and the technical replicate number separated by an underscore. Author defined Forty-three antimicrobial resistance gene targets were assessed in this study: aminoglycoside resistance genes (aac3-IV, aac(6’)-IIc, aacA43, APH, rmtB), beta-lactam resistance genes (blaCMY, blaGES, blaIMP, blaKPC, blaNDM, blaOXA, blaOXY, blaSHV, blaTEM, blaVIM, blaCTXM), class one integrons (dfrA1, int1), multidrug efflux pumps (qacEdelta, mepR, mexF), glycopeptide resistance genes (vanA, vanD), macrolide resistance genes (ereA, ermB, ermD, ermF), mobile genetic elements (IS613), phenicol resistance genes (catII, catP), polymyxin resistance genes (mcr-1, mcr-2, mcr-3), quinolone resistance genes (oqxA, qnrB, qnrS), sulfonamide resistance genes (sul1, sul3), and tetracycline resistance genes (tetA, tetD, tetM, tetL, tetX). Microbial source trackers were also selected for humans (HF183), birds (GFD), and ruminants (Rum2Bac) and the universal bacteria marker (16S rRNA). Non template controls (NTC) were assessed with every sample. columns "1015b" through "SCCC137" Cycle threshold values for antimicrobial resistance genes measured with qPCR for bacterial isolate with the code indicated in the column header. Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. anitmicrobialResistance_surfaceWater_qPCR_bacterial_Isolates_wholeGen.csv Binary outcomes describing the detection of antimicrobial resistance genes within whole genome sequences of bacterial isolates using the Comprehensive Antibiotic Resistance Database. Presented in a Comma Separated Value (CSV) formatted table. Author defined GeneID The GeneID consists of the gene name that was assessed from whole genome sequences of bacterial isolates on the Comprehensive Antibiotic Resistance Database. Author defined Forty-three antimicrobial resistance gene targets were assessed by whole genome sequencing in this study: aminoglycoside resistance genes (aac3-IV, aac(6’)-IIc, aacA43, APH, rmtB), beta-lactam resistance genes (blaCMY, blaGES, blaIMP, blaKPC, blaNDM, blaOXA, blaOXY, blaSHV, blaTEM, blaVIM, blaCTXM), class one integrons (dfrA1, int1), multidrug efflux pumps (qacEdelta, mepR, mexF), glycopeptide resistance genes (vanA, vanD), macrolide resistance genes (ereA, ermB, ermD, ermF), mobile genetic elements (IS613), phenicol resistance genes (catII, catP), polymyxin resistance genes (mcr-1, mcr-2, mcr-3), quinolone resistance genes (oqxA, qnrB, qnrS), sulfonamide resistance genes (sul1, sul3), and tetracycline resistance genes (tetA, tetD, tetM, tetL, tetX). columns "1015b" through "SCCC137" Binary outcome of detection for an antimicrobial resistance gene for the bacterial isolate with the code indicated in the column header. Author defined 0 Gene target not detected Author defined 1 Gene target detected Author defined anitmicrobialResistance_surfaceWater_qPCR_resultsSpike.csv Results from a laboratory study where known bacteria where spiked into volumes of sterile water at three concentrations, filtered with ultrafiltration, and assessed with quantitative PCR (qPCR). Presented in a Comma Separated Value (CSV) formatted table. Author defined MeasurementID The name of the gene target measured in samples using qPCR and the technical replicate separated by an underscore. Author defined Forty-three antimicrobial resistance gene targets were assessed in this study: aminoglycoside resistance genes (aac3-IV, aac(6’)-IIc, aacA43, APH, rmtB), beta-lactam resistance genes (blaCMY, blaGES, blaIMP, blaKPC, blaNDM, blaOXA, blaOXY, blaSHV, blaTEM, blaVIM, blaCTXM), class one integrons (dfrA1, int1), multidrug efflux pumps (qacEdelta, mepR, mexF), glycopeptide resistance genes (vanA, vanD), macrolide resistance genes (ereA, ermB, ermD, ermF), mobile genetic elements (IS613), phenicol resistance genes (catII, catP), polymyxin resistance genes (mcr-1, mcr-2, mcr-3), quinolone resistance genes (oqxA, qnrB, qnrS), sulfonamide resistance genes (sul1, sul3), and tetracycline resistance genes (tetA, tetD, tetM, tetL, tetX). Microbial source trackers were also selected for humans (HF183), birds (GFD), and ruminants (Rum2Bac) and the universal bacteria marker (16S rRNA). Non template controls (NTC) were assessed with every sample. columns "1L_100cpl_Rep1" through "50L_10000cpl_Rep3" Cycle threshold values for antimicrobial resistance genes measured with qPCR for the first, second and third replicate of a 1, 10, 25, or 50 liter water sample spiked with bacterial cells at 100, 1000, or 10000 cells per liter. Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. anitmicrobialResistance_surfaceWater_qPCR_resultsSpike_melt.csv Results from a laboratory study where known bacteria where spiked into volumes of sterile water at three concentrations, filtered with ultrafiltration, and assessed with quantitative PCR (qPCR). Presented in a Comma Separated Value (CSV) formatted table. Author defined MeasurementID The name of the gene target measured in samples using qPCR and the technical replicate separated by an underscore. If the measurement is a melt temperature, the most prominent melting temperature is listed as "MeltTemp1" followed by "MeltTemp2" and "MeltTemp3". Author defined Forty-three antimicrobial resistance gene targets were assessed in this study: aminoglycoside resistance genes (aac3-IV, aac(6’)-IIc, aacA43, APH, rmtB), beta-lactam resistance genes (blaCMY, blaGES, blaIMP, blaKPC, blaNDM, blaOXA, blaOXY, blaSHV, blaTEM, blaVIM, blaCTXM), class one integrons (dfrA1, int1), multidrug efflux pumps (qacEdelta, mepR, mexF), glycopeptide resistance genes (vanA, vanD), macrolide resistance genes (ereA, ermB, ermD, ermF), mobile genetic elements (IS613), phenicol resistance genes (catII, catP), polymyxin resistance genes (mcr-1, mcr-2, mcr-3), quinolone resistance genes (oqxA, qnrB, qnrS), sulfonamide resistance genes (sul1, sul3), and tetracycline resistance genes (tetA, tetD, tetM, tetL, tetX). Microbial source trackers were also selected for humans (HF183), birds (GFD), and ruminants (Rum2Bac) and the universal bacteria marker (16S rRNA). Non template controls (NTC) were assessed with every sample. columns "1L_100cpl_Rep1" through "50L_10000cpl_Rep3" Melt temperatures for antimicrobial resistance genes measured with qPCR for the first, second and third replicate of a 1, 10, 25, or 50 liter water sample spiked with bacterial cells at 100, 1000, or 10000 cells per liter. Blank cells indicate that no data are available. Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. anitmicrobialResistance_surfaceWater_qPCR_measurementsNP.csv Antimicrobial resistance genes assessed with quantitative PCR (qPCR) from 20 liter and 50 liter surface water samples collected from three national parks in Alaska (Denali National Park and Preserve, Kenai Fjords National Park, and Wrangell-St. Elias National Park and Preserve). Presented in a Comma Separated Value (CSV) formatted table. Author defined MeasurementID The name of the gene target measured in samples using qPCR and the technical replicate separated by an underscore. Author defined Forty-three antimicrobial resistance gene targets were assessed in this study: aminoglycoside resistance genes (aac3-IV, aac(6’)-IIc, aacA43, APH, rmtB), beta-lactam resistance genes (blaCMY, blaGES, blaIMP, blaKPC, blaNDM, blaOXA, blaOXY, blaSHV, blaTEM, blaVIM, blaCTXM), class one integrons (dfrA1, int1), multidrug efflux pumps (qacEdelta, mepR, mexF), glycopeptide resistance genes (vanA, vanD), macrolide resistance genes (ereA, ermB, ermD, ermF), mobile genetic elements (IS613), phenicol resistance genes (catII, catP), polymyxin resistance genes (mcr-1, mcr-2, mcr-3), quinolone resistance genes (oqxA, qnrB, qnrS), sulfonamide resistance genes (sul1, sul3), and tetracycline resistance genes (tetA, tetD, tetM, tetL, tetX). Microbial source trackers were also selected for humans (HF183), birds (GFD), and ruminants (Rum2Bac) and the universal bacteria marker (16S rRNA). Non template controls (NTC) were assessed with every sample. columns "DENA_1Rep1_20L" through "DENA_1Rep3_20L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Rock Creek in Denali National Park and Preserve (approximately 63.72N 148.96W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "DENA_1Rep1_50L" through "DENA_1Rep2_50L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Rock Creek in Denali National Park and Preserve (approximately 63.72N 148.96W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "DENA_2Rep1_20L" through "DENA_2Rep3_20L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Riley Creek in Denali National Park and Preserve (approximately 63.73N 148.89W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "DENA_2Rep1_50L" through "DENA_2Rep2_50L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Riley Creek in Denali National Park and Preserve (approximately 63.73N 148.89W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "KEFJ_1Rep1_20L" through "KEFJ_1Rep3_20L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Exit Glacier Creek in Kenai Fjords National Park (approximately 60.18N 149.63W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "KEFJ_2Rep1_20L" through "KEFJ_2Rep3_20L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Surprise Glacier Creek in Kenai Fjords National Park (approximately 59.85N 149.87W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "KEFJ_2Rep1_50L" through "KEFJ_2Rep2_50L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Surprise Glacier Creek in Kenai Fjords National Park (approximately 59.85N 149.87W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "WRST_1Rep1_20L" through "WRST_1Rep3_20L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Jumbo Creek in Wrangell-St. Elias National Park (approximately 61.50N 142.89W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "WRST_1Rep1_50L" through "WRST_1Rep2_50L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Jumbo Creek in Wrangell-St. Elias National Park (approximately 61.50N 142.89W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "WRST_2Rep1_20L" through "WRST_2Rep3_20L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from National Creek in Wrangell-St. Elias National Park (approximately 61.48N 142.88W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. columns "WRST_2Rep1_50L" through "WRST_2Rep2_50L" Cycle threshold values for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from National Creek in Wrangell-St. Elias National Park (approximately 61.48N 142.88W). Author defined Numerical values indicate at which cycle the fluorescence crossed a pre-determined threshold for detection. Gene targets listed as "Undetermined" did not amplify across the threshold for positive detection. anitmicrobialResistance_surfaceWater_qPCR_measurementsNP_melt.csv Antimicrobial resistance genes assessed with quantitative PCR (qPCR) from 20 liter and 50 liter surface water samples collected from three national parks in Alaska (Denali National Park and Preserve, Kenai Fjords National Park, and Wrangell-St. Elias National Park and Preserve). Presented in a Comma Separated Value (CSV) formatted table. Author defined MeasurementID The name of the gene target measured in samples using qPCR and the technical replicate separated by an underscore. If the measurement is a melt temperature, the most prominent melting temperature is listed as "MeltTemp1" followed by "MeltTemp2" and "MeltTemp3". Author defined Forty-three antimicrobial resistance gene targets were assessed in this study: aminoglycoside resistance genes (aac3-IV, aac(6’)-IIc, aacA43, APH, rmtB), beta-lactam resistance genes (blaCMY, blaGES, blaIMP, blaKPC, blaNDM, blaOXA, blaOXY, blaSHV, blaTEM, blaVIM, blaCTXM), class one integrons (dfrA1, int1), multidrug efflux pumps (qacEdelta, mepR, mexF), glycopeptide resistance genes (vanA, vanD), macrolide resistance genes (ereA, ermB, ermD, ermF), mobile genetic elements (IS613), phenicol resistance genes (catII, catP), polymyxin resistance genes (mcr-1, mcr-2, mcr-3), quinolone resistance genes (oqxA, qnrB, qnrS), sulfonamide resistance genes (sul1, sul3), and tetracycline resistance genes (tetA, tetD, tetM, tetL, tetX). Microbial source trackers were also selected for humans (HF183), birds (GFD), and ruminants (Rum2Bac) and the universal bacteria marker (16S rRNA). Non template controls (NTC) were assessed with every sample. columns "DENA_1Rep1_20L" through "DENA_1Rep3_20L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Rock Creek in Denali National Park and Preserve (approximately 63.72N 148.96W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "DENA_1Rep1_50L" through "DENA_1Rep2_50L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Rock Creek in Denali National Park and Preserve (approximately 63.72N 148.96W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "DENA_2Rep1_20L" through "DENA_2Rep3_20L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Riley Creek in Denali National Park and Preserve (approximately 63.73N 148.89W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "DENA_2Rep1_50L" through "DENA_2Rep2_50L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Riley Creek in Denali National Park and Preserve (approximately 63.73N 148.89W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "KEFJ_1Rep1_20L" through "KEFJ_1Rep3_20L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Exit Glacier Creek in Kenai Fjords National Park (approximately 60.18N 149.63W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "KEFJ_2Rep1_20L" through "KEFJ_2Rep3_20L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Surprise Glacier Creek in Kenai Fjords National Park (approximately 59.85N 149.87W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "KEFJ_2Rep1_50L" through "KEFJ_2Rep2_50L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Surprise Glacier Creek in Kenai Fjords National Park (approximately 59.85N 149.87W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "WRST_1Rep1_20L" through "WRST_1Rep3_20L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from Jumbo Creek in Wrangell-St. Elias National Park (approximately 61.50N 142.89W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "WRST_1Rep1_50L" through "WRST_1Rep2_50L" Melt temperatures values for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from Jumbo Creek in Wrangell-St. Elias National Park (approximately 61.50N 142.89W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "WRST_2Rep1_20L" through "WRST_2Rep3_20L" Melt temperatures values for antimicrobial resistance genes assessed by qPCR from the first, second, and third replicate of a 20 liter sample collected from National Creek in Wrangell-St. Elias National Park (approximately 61.48N 142.88W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. columns "WRST_2Rep1_50L" through "WRST_2Rep2_50L" Melt temperatures for antimicrobial resistance genes assessed by qPCR from the first and second replicate of a 50 liter sample collected from National Creek in Wrangell-St. Elias National Park (approximately 61.48N 142.88W). Author defined Melt temperature equals the temperature, in degrees Celsius, at which the PCR amplicon dissociates. Multiple melting temperatures can be detected per sample, and the top three are recorded here as MeltTemp1, MeltTemp2, and MeltTemp3 for each replicate. “NA” indicates that a measurement was not taken for that sample. U.S. Geological Survey USGS ScienceBase Team Mailing and Physical

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Denver Colorado 80225 USA 1-888-275-8747 sciencebase@usgs.gov The U.S. Geological Survey, Alaska Science Center is the authoritative source of these data, distributed by ScienceBase (a USGS Trusted Digital Repository). Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey, no warranty expressed or implied is made regarding the display or utility of the data for other purposes or on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. CSV Tabular data in CSV format; FGDC metadata in XML and HTML formats. https://doi.org/10.5066/P14G9MJW None 20250421 U.S. Geological Survey, Alaska Science Center Mailing and Physical

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Anchorage Alaska 99508 USA 907-786-7000 gs-ak_asc_datamanagers@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata (CSDGM) FGDC-STD-001.1-1999