Lisa M. McBride Andrea F. Currylow Gretchen E. Anderson Jacquelyn C. Guzy Amy A. Yackel Adams 20250408 Digital Video https://doi.org/10.5066/P13GCMCY Select photographic sequence of a Burmese Python (Python bivittatus) nesting in the wild within a concrete pipe from 01 June 2022 through 01 August 2022 in Big Cypress National Preserve within the Greater Everglades Ecosystem, Florida, USA. This python was tracked to an area of abandoned construction pipes and discovered inside to have oviposited. Throughout incubation, the python was observed to shift her coils, shiver to elevate body temperature, and yawns. Twice the wildlife camera captures a Virginia opossum (Didelphis virginiana) entering the pipe and quickly exiting. The python temporarily leaves her clutch after biologists enclose the nest pipe. After returning on top of her nest, she lays coiled around the nest for 12 more days, she eventually uncoils and leaves the clutch. Six days later, the clutch begins to hatch in the pipe and continued to hatch over the next 13 days. The hatching of this clutch has been documented as largest clutch size ever documented for a free-ranging Burmese Python which adds to our expanding knowledge of this invasive species. Photos were captured by U.S. Geological Survey equipment (Hyperfire 2, Reconyx, Holmen, Wisconsin, USA). Additional csv dataset included showcases range of temperatures in celsius collected by three sets of data loggers (IButton DS1922L Thermochron Data Logger) at three different locations. Each data logger set included one 11-bit data logger collecting a temperature once every 20 mins and one 8-bit data logger collecting temperature once every 40 minutes. The 11-bit data logger recorded temperature to three decimal places and the 8-bit data logger recorded temperature to one decimal place. One set of data loggers were placed inside the coils of the nesting female Burmese python, second set was placed 0 centimeters adjacent to the nesting python, and the third set was placed 40 centimeters adjacent to the nesting python. These data were collected as part of another study on telemetered Burmese pythons. Appropriate use would be to visually supplement the published account. 20220601 20220801 ground condition Complete None planned Big Cypress National Preserve within the Greater Everglades Ecosystem, Florida USA -81.3338 -81.1107 26.0352 25.8611 ISO 19115 Topic Category biota None Burmese python Snake nest Nest hatching USGS Thesaurus invasive species herpetology USGS Metadata Identifier USGS:67006386d34e80be174aeb9a None Greater Everglades Florida Big Cypress National Preserve South Florida None Burmese Python Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Reptilia Order Squamata Suborder Serpentes Infraorder Alethinophidia Family Pythonidae Genus Python Species Python bivittatus TSN: 1094050 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Amy A. Yackel Adams USGS - ROCKY MOUNTAIN REGION Supervisory Research Ecologist mailing and physical

2150 Centre Ave., Bldg C.

Fort Collins CO 80526 970-226-9489 yackela@usgs.gov USGS Greater Everglades Priority Ecosystem Science (GEPES) Program and USGS Ecosystem Mission Area Biological Threats and Invasive Species Program All data were entered by those collecting the data and then proofed by other project personnel, the project biometrician, and analyst. No erroneous outliers or duplicate entries are contained in the data set. This dataset is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. Photos were captured by U.S. Geological Survey equipment (Hyperfire 2, Reconyx, Holmen, Wisconsin, USA) in Big Cypress National Preserve within the Greater Everglades Ecosystem, Florida USA. The brooding python was found on top of her nest as part of another study on telemetered Burmese pythons. The camera was set three feet away from the brooding Burmese python and at ground level. To create the mp4 file, we imported all of the photos from the wildlife camera and selected photos that captured key nesting and hatching behavior of Burmese pythons. We imported those select photos into Adobe software (Premiere Pro, version 24.4) in chronological order to create a video timeline. We then exported the video from the program into .mp4 format. 20240920 Temperature data in celsius was collected by three sets of data loggers (IButton DS1922L Thermochron Data Logger) in Big Cypress National Preserve within the Greater Everglades Ecosystem, Florida USA. The brooding python was found on top of her nest as part of another study on telemetered Burmese pythons. Prior to the data loggers being deployed in the field, they were programmed using Java software (OneWireViewer, version 1.6). There were six data loggers in total. Three data loggers were 8-bit and programmed to collect temperature once every 40 minutes and three data loggers were 11-bit and programmed to collect temperature once every 20 minutes. A set of data loggers were paired together which included one of the 8-bit and one of the 11-bit. The sets of data loggers were coated in a red rubber coating to make the data loggers water resistant in the field. One set of data loggers were placed at "in clutch" location which was inside the nesting female Burmese python's coils to record temperature in celsius. A second set of data loggers were placed at "clutch proximal" which was 0 centimeters adjacent to the coils of the brooding python. A third set of data loggers were placed at "clutch distal" which was 40 centimeters adjacent to the coils of the brooding python. Temperature data was then extracted from the data loggers using Java software (OneWireViewer, version 1.6). Temperature data was then compiled into a csv file. 20241120 NestingPython_iButtonData_2022.csv Comma Separated Value (CSV) file containing temperature data collected by 3 sets of data loggers placed in and adjacent to a nesting female Burmese python. Producer Defined Date Date (formatted as M/DD/YYYY) that data logger recorded individual temperature reading. Producer Defined The values recorded in the date field are the range of dates that data loggers were collecting temperature data in celsius. The date range is from June 01 to July 26, 2022. Time Time (formatted as HH:MM:SS) at which the data logger recorded the temperature reading. Producer Defined 10:02:01 AM 9:55:01 PM second TemperatureCelsius Temperature in celsius that data logger recorded. Producer Defined 22.5 34.063 celsius DataloggerLocation Location that data logger was positioned, there were 3 different locations. Producer Defined Clutch Distal Location of data loggers placed 40 centimeters adjacent to nesting female Burmese python. Producer defined Clutch Proximal Location of data loggers placed 0 centimeters adjacent to nesting female Burmese python. Producer defined In Clutch Location of data loggers placed inside the coils of the nesting female Burmese python. Producer defined DataloggerSampleRate The two different time intervals at which the data logger collected temperature data in celsius. Producer Defined 1 x 20 mins Data logger recorded temperature in celsius once every 20 minutes from June 01 2022 to July 26 2022. Producer defined 1 x 40 mins Data logger recorded temperature in celsius once every 40 minutes from June 01 2022 to July 26 2022. Producer defined BroodingPython.mp4: Entire photo-documented sequence from 01 June 2022 to 01 August 2022 showing nesting behavior of an adult Burmese python (Python bivittatus) that laid 96 eggs in the wild within a concrete pipe in Big Cypress National Preserve within the Greater Everglades Ecosystem, Florida, USA. Not applicable GS ScienceBase U.S. Geological Survey mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Digital Data https://doi.org/10.5066/P13GCMCY None 20250408 FORT Data Management U.S. Geological Survey, Fort Collins Science Center FORT Data Management mailing and physical

2150 Centre Avenue Bldg C

Fort Collins CO 80526 US 970-226-9100 970-226-9230 fortdatamanagement@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999