Ellen J. Dunkle Kylle Roy Roshan Manandhar Michelle Clark Kalli Harshman Robert W. Peck 20231129 tabular digital data https://doi.org/10.5066/P9SY9RUX Roy, Kylle Dunkle, Ellen J. Manandhar, Roshan Clark, Michelle Magnacca, Karl N. Harshman, Kalli Peck, Robert W. 2024 publication 56:61–71 N/A Proceedings of the Hawaiian Entomological Society https://doi.org/10125/110083 These data include bark and ambrosia beetle (Coleoptera: Curculionidae) species directly reared from ʻōhiʻa lehua (Metrosideros polymorpha) wood infected with either Ceratocystis lukuohia or Ceratocystis huliohia, the pathogens responsible for Rapid ʻŌhiʻa Death (ROD). Rearing took place on Kauaʻi between January 2020 and November 2021. Data were collected to understand the ambrosia beetle communities associated with rapid ʻōhiʻa death (ROD) on Kauaʻi and to inform disease management decisions on the island. 202001 20211229 observed Complete None planned Kauaʻi Island -159.7604 -159.2894 22.2281 21.8806 ISO 19115 Topic Category biota None Ceratocystis rapid ohia death ambrosia beetle frass Hawaii ohia USGS Metadata Identifier USGS:655808bdd34ee4b6e05c642f None Ceratocystis rapid ohia death Hawaii ambrosia beetle frass ohia None Xyleborus Metrosideros polymorpha Ceratocystis Domain Eukaryota Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda Class Insecta Subclass Pterygota Infraclass Neoptera Superorder Holometabola Order Coleoptera Suborder Polyphaga Infraorder Cucujiformia Superfamily Curculionoidea Family Curculionidae Subfamily Scolytinae Genus Xyleborus TSN: 188139 Kingdom Plantae Subkingdom Viridiplantae Infrakingdom Streptophyta Superdivision Embryophyta Division Tracheophyta Subdivision Spermatophytina Class Magnoliopsida Superorder Rosanae Order Myrtales Family Myrtaceae Genus Metrosideros Species Metrosideros polymorpha TSN: 27259 Kingdom Fungi Subkingdom Dikarya Division Ascomycota Subdivision Pezizomycotina Class Sordariomycetes Subclass Sordariomycetidae Order Ophiostomatales Family Ophiostomataceae Genus Ceratocystis TSN: 181740 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. U.S. Geological Survey-PIERC Data Steward physical

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Hawaii Natl Park HI 96718 USA 808-985-6420 pierc-datasteward@usgs.gov This work was supported by grants from the Hawaiʻi Department of Land and Natural Resources and the U.S. Forest Service. We thank Dr. Lisa Keith and Eva Brill (United States Department of Agriculture-Agricultural Research Services-Daniel K. Inouye-U.S. Pacific Basin Agricultural Research Center) for diagnostic testing services. We also thank Kauaʻi Division of Forestry and Wildlife (DOFAW) and Kauaʻi Invasive Species Committee (KISC) crews for sampling and collecting trees for rearing. Microsoft 365 Excel Version 2022, Windows 10 Enterprise All insects were identified to species and verified with visual inspection utilizing a dissection microscope. All data are logical; the data was visually reviewed for logical consistency. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details No spatial data reported. No spatial data reported. Bolts were collected from previously tested ʻōhiʻa lehua (Metrosideros polymorpha) trees throughout Kauaʻi Island, ranging between 138-910 m in elevation. Testing for the presence of Ceratocystis lukuohia and C. huliohia DNA was conducted via quantitative polymerase chain reaction (qPCR) (Heller and keith 2018) at United States Department of Agriculture-Agricultural Research Services-Daniel K. Inouye-U.S. Pacific Basin Agricultural Research Center (USDA-ARS-DKI-PBARC) in Hilo, HI from samples collected by Kauaʻi Division of Forestry and Wildlife (DOFAW) and Kauaʻi Invasive Species Committee (KISC) crews. Upon positive DNA confirmation, DOFAW and KISC returned to the infected trees and randomly selected a ~50 cm-long portion of the stem to be cut and transferred to the lab for rearing insects contained in the bolt between January 2020 and November 2021. Bolts were transported to the lab and placed in rearing chambers consisting of pop-up mesh terrarium cages (60 x 40 cm) on top of white computer paper for 3-4 months. References: Heller, W. P. and L. M. Keith. 2018. Real-time PCR assays to detect and distinguish the Rapid Ohia Death pathogens Ceratocystis lukuohia and C. huliohia. Phytopathology. 108: 1395–1401. Roy, K., K.A. Jaenecke, and R.W. Peck. 2020. Ambrosia beetle (Coleoptera: Curculionidae) communities and frass production in 'Ōhi'a (Myrtales: Myrtaceae) infected with ceratocystis (Microascales: Ceratocystidaceae) fungi responsible for rapid 'Ōhi'a death. Environmental Etnomology 49: 1345-1354. DOI: 10.1093/ee/nvaa108 Samuelson, G. 1981. A synopsis of Hawaiian Xyleborini (Coleoptera: Scolytidae). Pacific Insects. 23: 50–92. 20211229 Beetles that emerged from bolts were collected bi-weekly with forceps and stored in glass vials with 70% ethanol for identification via visual inspection with a dissection microscope in accordance with Samuelson 1981 dichotomous key. References: Samuelson, G. 1981. A synopsis of Hawaiian Xyleborini (Coleoptera: Scolytidae). Pacific Insects. 23: 50–92. 20211229 Vector Entity point 10 Mercator 0.0 0.0 0.0 0.0 coordinate pair 0.6096 0.6096 meters unknown Unknown 6378137.0 0.1 Kauai_Reared_Beetles.csv Comma Separated Value (CSV) file containing data. Producer Defined Start_Date Date when bolt (tree stem section) monitoring began Producer Defined 01/30/2020 06/29/2021 days 1 End_Date Date when beetles were collected from bolts (tree stem sections) for the final time. Producer Defined 4/30/2020 12/29/2021 days 1 Site Descriptive locations of where trees from which bolts (tree stem sections) were taken originated on Kauaʻi. Producer Defined Private Property Kahili 1 Private property in Kahili, HI. Producer defined Hanalei Site located in Hanalei, HI. Producer defined North Powerline Site located near northern section of Powerline Rd, HI. Producer defined CTAHR Site located at College of Tropical Agriculture and Human Resources Extension office in Līhue, HI. Producer defined Private Property Omao Private peoperty located in Omao, HI. Producer defined Kokeʻe Nualolo Site located along Nualolo trail in Kokeʻe State Park, HI. Producer defined Moalepe Trail Site located along Moalepe Trail on east Kauaʻi, HI. Producer defined Powerline Resevoir Site located on Powerline Rd, HI. Producer defined Private Property Kahili 2 Private property in Kahili, HI. Producer defined Anahola Site located in Anahola, HI Producer defined Kalalau Valley Site located in Kalalau Valley in the Nā Pali Wilderness State Park. Producer defined Private Property Wailua Homesteads Private Property located in Wailua Homesteads, HI. Producer defined Lawai Mauka Koloa-Lihue Forest Reserve Site located within Līhue-Kolea Forest Reserve, HI. Producer defined Private Property Wailua Private property located in Wailua, HI. Producer defined ROD_species Species of rapid ʻōhiʻa death (ROD)-causing fungal DNA found in bolt (tree stem section). Producer Defined C.lukuohia Ceratocystis lukuohia, one of the fungi that can cause ROD. Producer defined C.huliohia Ceratocystis huliohia, one of the fungi that can cause ROD. Producer defined Elevation.m Elevation in meters where bolt (tree stem section) originated. Producer Defined 138.5 910.9 meters .1 Bolt.Name Descriptive title given to each bolt (tree stem section). Producer Defined Each bolt was given a unique name starting with the year, month, and day when tree was originally identified as ROD-symptomatic and tested for Ceratocysts DNA. Following these numbers include the collectors initials and the number of the sample. X.perforans Count of the beetle species Xyleborus perforans (Curculionidae: Xyleborini) reared. Producer Defined 0 227 count 1 X.ferrugineus Count of the beetle species Xyleborus ferrugineus (Curculionidae: Xyleborini) reared. Producer Defined 0 108 count 1 Xi.saxesenii Count of the beetle species Xyleborinus saxesenii (Curculionidae: Xyleborini) reared. Producer Defined 0 48 count 1 Xa.crassiusculus Count of the beetle species Xylosandrus crassiusculus (Curculionidae: Xyleborini) reared. Producer Defined 0 11 count 1 X.affinis Count of the beetle species Xyleborus affinis (Curculionidae: Xyleborini) reared. Producer Defined 0 2 count 1 C.externedentatus Count of the beetle species Crossotarsus externedentatus (Curculionidae: Platypodinae) reared. Producer Defined 0 59 count 1 Laemophloeidae Count of beetles belonging to family Lemphloeidae (flat bark beetles) reared. Producer Defined 0 2 count 1 U.S. Geological Survey GS ScienceBase mailing address

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Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P9SY9RUX None 20250307 U.S. Geological Survey-PIERC Data Steward physical

Bldg 344 Chain of Craters Rd.

Hawaii Natl Park HI 96718 US 808-985-6420 pierc-datasteward@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999