Sergio A. Sabat-Bonilla Sally Entrekin Abigail Belvin Gregory B. Noe 20231201 spreadsheet https://doi.org/10.5066/P9H41UDY Gregory B. Noe 20220818 vector digital data https://www.sciencebase.gov/catalog/item/62fea68ed34e3a4442876167 From 20 May - 8 June 2021, a Virginia Tech team of 2-3 sampled the macroinvertebrate community in 30 Shenandoah Valley streams (Virginia and West Virginia, USA) as part of a larger stream-health study including other teams who surveyed geomorphology, water quality, flow, temperature,fish community, and fish health at the same 30 streams. The macroinvertebrate community team also characterized the habitat units sampled for macroinvertebrates. At each stream, we sampled macroinvertebrates in two ways:1) in each stream we took 5 slack samples in riffles according to NAWQA protocol, and 2) we selected the most available and productive habitat where we used the Slack sampler to collect 5 additional samples in wood debris accumulations, undercut banks or leaf packs. Mesh size was 250 micrometer and samples were all taken for a known area. Other measurements included water temperature, electrical conductivity, and clarity, and percent of the reach comprising pool, riffle, and run habitats. These macroinvertebrate community data were collected as part of a larger interdisciplinary Shenandoah Valley field campaign (https://www.sciencebase.gov/catalog/item/636a6018d34ed907bf6a684f) to evaluate the effect of agricultural land use and conservation practices on holistic stream health. 20210520 20210806 ground condition In work None planned Shenandoah Valley, Virginia and West Virginia, USA -79.4352 -77.9275 39.5693 37.6789 ISO 19115 Topic Category biota USGS Aquatic Macroinvertebrates field sampling river systems freshwater ecosystems community ecology USGS Metadata Identifier USGS:64e4f07ad34e736b81e1861b CommonGeographicAreas Shenandoah United States Virginia West Virginia Potomac Rockbridge Augusta Rockingham Warren Clarke Jefferson Berkeley USGS Ephemeroptera Baetidae Baetiscidae Caenidae Ephemerellidae Ephemeridae Heptageniidae Leptophlebiidae Odonata Aeshnidae Calopterygidae Cordulegastridae Gomphidae Plecoptera Leuctridae Nemouridae Perlidae Perlodidae Taeniopterygidae Orthoptera Hemiptera Corixidae Lepidoptera Crambidae Megaloptera Corydalidae Sialidae Trichoptera Brachycentridae Goeridae Helicopsychidae Hydropsychidae Hydroptilidae Leptoceridae Limnephilidae Philopotamidae Polycentropodidae Rhyacophilidae Coleoptera Curculionidae Dryopidae Elmidae Gyrinidae Haliplidae Hydrophilidae Psephenidae Scirtidae Diptera Athericidae Ceratopogonidae Dixidae Empididae Psychodidae Simuliidae Stratiomyidae Tabanidae Tipulidae Amphipoda Crangonyctidae Bivalvia Cyrenidae Sphaeriidae Decapoda Cambaridae Gastropoda Ancylidae Physidae Hydrobiidae Lymnaeidae Planorbidae Pleuroceridae Hexapoda Collembola Trombidiformes Isopoda Asellidae Oligochaeta Chironomidae Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda TSN: 563886 Class Insecta Subclass Pterygota Infraclass Palaeoptera Order Ephemeroptera TSN: 100502 Suborder Pisciforma Family Baetidae TSN: 100755 Superfamily Heptagenioidea Family Heptageniidae TSN: 100504 Suborder Carapacea Family Baetiscidae TSN: 101493 Suborder Furcatergalia Infraorder Pannota Superfamily Caenoidea Family Caenidae TSN: 101467 Superfamily Ephemerelloidea Family Ephemerellidae TSN: 101232 Infraorder Scapphodonta Superfamily Ephemeroidea Family Ephemeridae TSN: 101525 Infraorder Lanceolata Family Leptophlebiidae TSN: 101095 Order Odonata TSN: 101593 Suborder Anisoptera Family Aeshnidae TSN: 101596 Family Cordulegastridae TSN: 102026 Family Gomphidae TSN: 101664 Suborder Zygoptera Family Calopterygidae TSN: 102043 Infraclass Neoptera Superorder Polyneoptera Order Plecoptera TSN: 102467 Suborder Euholognatha Family Leuctridae TSN: 102840 Family Nemouridae TSN: 102517 Family Taeniopterygidae TSN: 102788 Suborder Systellognatha Family Perlidae TSN: 102914 Family Perlodidae TSN: 102994 Order Orthoptera TSN: 102160 Superorder Paraneoptera Order Hemiptera TSN: 103359 Suborder Heteroptera Infraorder Nepomorpha Superfamily Corixoidea Family Corixidae TSN: 103364 Superorder Holometabola Order Lepidoptera TSN: 117232 Superfamily Pyraloidea Family Crambidae TSN: 693963 Order Trichoptera TSN: 115095 Superfamily Limnephiloidea Family Brachycentridae TSN: 116905 Family Goeridae TSN: 115934 Family Limnephilidae TSN: 115933 Superfamily Sericostomatoidea Family Helicopsychidae TSN: 117015 Superfamily Hydropsychoidea Family Hydropsychidae TSN: 115398 Family Polycentropodidae TSN: 117043 Superfamily Hydroptiloidea Family Hydroptilidae TSN: 115629 Superfamily Leptoceroidea Family Leptoceridae TSN: 116547 Superfamily Philopotamoidea Family Philopotamidae TSN: 115257 Superfamily Rhyacophiloidea Family Rhyacophilidae TSN: 115096 Order Coleoptera TSN: 109216 Suborder Polyphaga Infraorder Cucujiformia Superfamily Curculionoidea Family Curculionidae TSN: 114666 Infraorder Elateriformia Superfamily Byrrhoidea Family Dryopidae TSN: 113999 Family Elmidae TSN: 114093 Family Psephenidae TSN: 114069 Superfamily Scirtoidea Family Scirtidae TSN: 113924 Infraorder Staphyliniformia Superfamily Hydrophiloidea Family Hydrophilidae TSN: 112811 Suborder Adephaga Family Gyrinidae TSN: 112653 Family Haliplidae TSN: 111857 Order Diptera TSN: 118831 Suborder Brachycera Infraorder Tabanomorpha Family Athericidae TSN: 130928 Family Tabanidae TSN: 130934 Infraorder Muscomorpha Family Empididae TSN: 135830 Infraorder Stratiomyomorpha Family Stratiomyidae TSN: 130150 Suborder Nematocera Infraorder Culicomorpha Family Ceratopogonidae TSN: 127076 Family Dixidae TSN: 125809 Family Simuliidae TSN: 126640 Family Chironomidae TSN: 127917 Infraorder Psychodomorpha Family Psychodidae TSN: 125351 Infraorder Tipulomorpha Family Tipulidae TSN: 118840 Superorder Neuropterida Order Megaloptera TSN: 115000 Family Corydalidae TSN: 115023 Family Sialidae TSN: 115001 Class Collembola Order Collembola TSN: 99237 Subphylum Crustacea Class Malacostraca Subclass Eumalacostraca Superorder Peracarida Order Amphipoda TSN: 93294 Suborder Gammaridea Family Crangonyctidae TSN: 95080 Order Isopoda TSN: 92120 Suborder Asellota Superfamily Aselloidea Family Asellidae TSN: 92657 Superorder Eucarida Order Decapoda TSN: 95599 Suborder Pleocyemata Infraorder Astacidea Superfamily Astacoidea Family Cambaridae TSN: 97336 Subphylum Chelicerata Class Euchelicerata Subclass Arachnida Superorder Acariformes Order Trombidiformes TSN: 82769 Superphylum Spiralia Phylum Mollusca Class Bivalvia TSN: 79118 Subclass Autobranchia Infraclass Heteroconchia Order Venerida Superfamily Cyrenoidea Family Cyrenidae TSN: 1204699 Order Sphaeriida Superfamily Sphaerioidea Family Sphaeriidae TSN: 81389 Class Gastropoda TSN: 69459 Subclass Orthogastropoda Infraclass Heterobranchia Superorder Hygrophila Order Lymnaeida Superfamily Planorboidea Family Planorbidae TSN: 76591 Family Physidae TSN: 76676 Superfamily Lymnaeoidea Family Lymnaeidae TSN: 76483 Infraclass Caenogastropoda Order Littorinida Suborder Rissoidina Superfamily Truncatelloidea Family Hydrobiidae TSN: 70493 Order Cerithiida Superfamily Cerithioidea Family Pleuroceridae TSN: 71541 Phylum Annelida Class Clitellata None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Sally Entrekin Virginia Tech, Department of Entomology Associate Professor mailing

Price Hall, Room 216A

Blacksburg VA 24061-0321 USA 540-231-5978 sallye@vt.edu Spreadsheet Software. Data "Typology1_Macro_Taxa_Count_Complete.csv" size is 2.3MB, CSV Document Macroinvertebrates collected in the field were identified to the lowest possible taxonomic level (typically genera) based on taxonomic keys for the region in the lab. Data validation rules and drop-down lists implemented in spreadsheet software ensured that only valid values were entered. All data columns were checked to confirm that values fell within expected ranges. The dataset contains records of aquatic macroinvertebrate taxon counts at the specified sites. Sampling took place in 30 stream reaches across the Shenandoah River Valley, VA, with stream catchments selected as part of a gradient of agricultural landuse. Selected streams were grouped together into categories of high, medium, and low agriculture and BMP intensity. Streams were selected to be low gradient and of suitable length to conduct macroinvertebrate sampling (300m overall reach minimum) following regional sampling protocols (i.e., NAWQA and MBSS). Quantitative samples of benthic macroinvertebrates were collected at five riffles using a D-frame kick net. The sampling effort was 1-2 minutes over a 0.5m2 length of habitat. When only wood was available, a comparable area was scrubbed, slurry sieved, and saved. All samples were saved individually and preserved in 95% ethanol (EtOH). Macroinvertebrates have been identified to the lowest taxonomic value (e.g., genera and tribe) in the lab using established keys. For the analysis of our data, we utilized code from the ICPRB (Interstate Commission on the Potomac River Basin) to calculate the macroinvertebrate metrics and Chessie B-IBI scores for the 30 sites in Typology 1 of this project, as provided by Rikke D. Jepsen (rjepsen@icprb.org) from the Interstate Commission on the Potomac River Basin and Zachary M. Smith (zachary.smith2@dec.ny.gov) from the New York State Department of Environmental Conservation. We utilized the ICPRB code to calculate the designated metrics and derive the overall Chessie-BIBI, adhering to regional standards for the Chesapeake Bay Watershed. An important aspect of the code is the inclusion of a rarefaction step. This step standardizes the count of individuals across different datasets, addressing sample count variability, which was shown to lead to bias in the results (Smith et al. 2017). To mitigate this bias, the ICPRB Chessie B-IBI scoring was simulated 100 times to ensure robust and accurate results by providing a mean and median score, along with the standard deviation from the 100 simulated scores. The calculated metrics were aggregated at Family or Genus levels, depending on the context of the analysis. Richness and diversity metrics, however, were calculated using taxa identified to the Genus level. In addition, the calculations for the Hilsenhoff Biotic Index (HBI) and Modified Average Score Per Taxon (ASPT_MOD) required the assignment of Tolerance Values (TVs) to each taxon. Taxa lacking an assigned TV were excluded from these calculations to ensure metric accuracy. This rigorous approach enhances the reliability and scientific validity of the Chessie BIBI process, aligning it with USGS protocols while maintaining the integrity of the research. provide a concise description of the Chessie BIBI process. 20210520 Sally Entrekin Virginia Tech, Department of Entomology Associate Professor mailing

Price Hall, Room 216A

Blacksburg VA 24061-0321 USA 540-231-5978 sallye@vt.edu Macros_Typ-1_ChessieB-IBI_Scores_Metrics.csv Comma Separated Value (CSV) file containing data. Producer Defined unique_id this column defines the site in a numerical format Producer Defined this column defines the site in a numerical format icprb_bioregion_id Bioregion of each site based on the ICPRB Producer Defined sgv Southern Great Valley Producer defined srv Southern Ridge and Valley Producer defined site_ID Site identifier Producer Defined Site identifier site_info Stream name Producer Defined Stream site information HUC12 Numeric Hydrologic Unit Code (HUC) 12 area in which stream reach is located. Producer Defined The U.S. is sub-divided into successively smaller hydrologic units which are classified in multiple levels and sub-units. Each unit is identified by a unique hydrologic unit code (HUC). Here the HUC12 is used as a regional location identifier of the sites. date Date of Sampling Event Producer Defined 5/27/21 Date of Sampling Event Producer defined 6/2/21 Date of Sampling Event Producer defined 5/21/21 Date of Sampling Event Producer defined 6/3/21 Date of Sampling Event Producer defined 6/8/21 Date of Sampling Event Producer defined 5/28/21 Date of Sampling Event Producer defined 5/20/21 Date of Sampling Event Producer defined 5/24/21 Date of Sampling Event Producer defined 5/25/21 Date of Sampling Event Producer defined 6/7/21 Date of Sampling Event Producer defined chessie_bibi Raw (non-rarified) Chesapeake Bay Index of Biotic Integrety (Chessie-BIBI) scores, meaning that these are directly taking 100 individuals from each stream to calculate metrics and score the stream. Rarefaction is used to test the range of scores based on the inclusion of rare taxa. Rarefied scores are provided should be used (i.e., median score) Producer Defined 6.235247476 88.55172767 rating Stream health ratings based on the Chessie B-IBI scores Producer Defined very_poor SRV_score$rating <- case_when( SRV_score$chessie_bibi < 21.58 ~ "very_poor", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi < 29.93 ~ "very_poor", TRUE ~ "ERROR") Producer defined poor SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 21.58 & SRV_score$chessie_bibi < 43.16 ~ "poor" SGV_score$rating <- case_when(SGV_score$chessie_bibi >= 29.93 & SGV_score$chessie_bibi < 59.86 ~ "poor", Producer defined fair SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 43.16 & SRV_score$chessie_bibi < 57.97 ~ "fair", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 59.86 & SGV_score$chessie_bibi < 66.02 ~ "fair", TRUE ~ "ERROR") Producer defined good SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 57.97 & SRV_score$chessie_bibi < 71.93 ~ "good", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 66.02 & SGV_score$chessie_bibi < 76.48 ~ "good", TRUE ~ "ERROR") Producer defined excellent SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 71.93 ~ "excellent", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 76.48 ~ "excellent", TRUE ~ "ERROR") Producer defined median_score Rarefied median Chessie BIBI scores from 100 repeats. An important aspect of the code is the inclusion of a rarefaction step. This step standardizes the count of individuals across different datasets, addressing sample count variability, which can lead to bias in the results. To mitigate this bias, the ICPRB suggests running the script 100 times to ensure robust and accurate results. Rarefied scores = median scores Producer Defined 2.884820126 88.55172767 median_rating Stream health ratings based on the median rarefied Chessie B-IBI scores Producer Defined poor SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 21.58 & SRV_score$chessie_bibi < 43.16 ~ "poor" SGV_score$rating <- case_when(SGV_score$chessie_bibi >= 29.93 & SGV_score$chessie_bibi < 59.86 ~ "poor", Producer defined good SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 57.97 & SRV_score$chessie_bibi < 71.93 ~ "good", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 66.02 & SGV_score$chessie_bibi < 76.48 ~ "good", TRUE ~ "ERROR") Producer defined very_poor SRV_score$rating <- case_when( SRV_score$chessie_bibi < 21.58 ~ "very_poor", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi < 29.93 ~ "very_poor", TRUE ~ "ERROR") Producer defined excellent SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 71.93 ~ "excellent", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 76.48 ~ "excellent", TRUE ~ "ERROR") Producer defined median_percent Percent of times the median score was obtained while running the rarefaction procedure. Producer Defined 54 100 mean_score Rarefied mean Chessie BIBI scores from 100 repeats. An important aspect of the code is the inclusion of a rarefaction step. This step standardizes the count of individuals across different datasets, addressing sample count variability, which can lead to bias in the results. To mitigate this bias, the ICPRB suggests running the script 100 times to ensure robust and accurate results. Encouraged to use median-scores instead of mean. Producer Defined 3.93749152 88.55172767 st_dev Standard deviation, a measure of variability between the rarefied 100 repeat scores. The 0 value for the standard deviation alludes to no change in scores across these 2 sites during this round of rarefaction, possible due to both sites containing greater quantities of taxa that would push the scoring to good scores. Producer Defined 0.0 4.069268579 mean_rating Stream health ratings based on the mean rarefied Chessie B-IBI scores Producer Defined poor SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 21.58 & SRV_score$chessie_bibi < 43.16 ~ "poor" SGV_score$rating <- case_when(SGV_score$chessie_bibi >= 29.93 & SGV_score$chessie_bibi < 59.86 ~ "poor", Producer defined good SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 57.97 & SRV_score$chessie_bibi < 71.93 ~ "good", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 66.02 & SGV_score$chessie_bibi < 76.48 ~ "good", TRUE ~ "ERROR") Producer defined very_poor SRV_score$rating <- case_when( SRV_score$chessie_bibi < 21.58 ~ "very_poor", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi < 29.93 ~ "very_poor", TRUE ~ "ERROR") Producer defined excellent SRV_score$rating <- case_when( SRV_score$chessie_bibi >= 71.93 ~ "excellent", TRUE ~ "ERROR") SGV_score$rating <- case_when( SGV_score$chessie_bibi >= 76.48 ~ "excellent", TRUE ~ "ERROR") Producer defined mean_percent Percent of times the mean score was obtained while running the rarefaction procedure. Producer Defined 54 100 aspt_mod Modified Average Score Per Taxon (ASPT_MOD) calculations required each taxon in a sample to have an assigned tolerance value (TV) Producer Defined 2.746755965 6.739823009 pct_gastro_oligo Percent abundance of Gastropd and Oligochaete Producer Defined 0.0 9.60960961 pct_diptera Percent abundance of the order Diptera Producer Defined 6.600344366 94.03432761 gold The sum of the percent abundance of gastropoda and oligochaeta plus percent abundance of diptera minus 100 Producer Defined 5.715714048 93.3805242 hbi The Hilsenhoff Biotic Index (HBI) calculations required each taxon in a sample to have an assigned tolerance value (TV) Producer Defined 3.324285714 6.645887273 pct_arthropoda Percent abundance of arthopods Producer Defined 89.88988989 100.0 pct_burrow Percent abundance of individuals with the habit classification of burrower Producer Defined 1.607305936 71.525831 pct_chironomidae Percent abundance of the Chironomidae family Producer Defined 1.534246575 71.40568682 pct_cling Percent abundance of individuals with the habit classification of clinger Producer Defined 12.01453989 77.95299938 pct_collect Percent abundance of individuals with the Functional Feeding Group (FFG) classification of Collector-Gatherers Producer Defined 67.2459893 99.74429224 pct_cote Percent abundance of "coleoptera", "trichoptera", "ephemeroptera", "odonata" Producer Defined 5.00456621 82.40643582 pct_ephemeroptera Percent abundance of Ephemeroptera Producer Defined 0.599900017 43.67861363 pct_baetidae Percent abundance of Ephemeroptera minus Baetidae family Producer Defined 0.38593482 36.90429303 pct_ephemeroptera_no_baetid Percent abundance of Ephemeroptera minus Baetidae family Producer Defined 0.066655557 36.73469388 pct_ept Percent abundance of Ephemeroptera, Plecoptera and Trichoptera Producer Defined 2.888846375 73.37534462 pct_hydropsychidae Percent abundance of Hydropsychidae family Producer Defined 0.219178082 43.57351509 pct_ept_no_hydro Percent abundance of Ephemeroptera, Plecoptera and Trichoptera minus Hydropsychidae family Producer Defined 11.9266055 98.92473118 pct_euholognatha Percent abundance of Euholognatha sub order within the Plecoptera order Producer Defined 0.0 24.18274911 pct_filter Percent abundance of individuals with the Functional Feeding Group (FFG) classification of Filter-feeder Producer Defined 1.970537593 61.42465753 pct_heptageniidae Percent abundance of Heptageniidae family Producer Defined 0.0 7.085561497 pct_hexapoda Percent abundance of Hexapoda subphylum Producer Defined 19.24622154 99.58617332 pct_hydro_ept Percent abundance of Hydropsychidae family divided by the percent abundance of Ephemeroptera, Plecoptera and Trichoptera Producer Defined 1.075268817 88.0733945 pct_intol_0_3 Percent abundance of taxa with Tolerance Value (TV) between 0 and 3 Producer Defined 0.146118721 47.45989305 pct_intol_0_4 Percent abundance of taxa with Tolerance Value (TV) between 0 and 4 Producer Defined 1.377463172 69.12170146 pct_amph_iso Percent abundance of "amphipoda", "isopoda" Producer Defined 0.0 76.77444041 pct_ephemerellidae Percent abundance of Ephemerellidae family Producer Defined 0.033327779 31.29251701 pct_limestone Percent abundance of Amphipoda and isopoda plus percent abundance of Ephemerellidae Producer Defined 0.033327779 76.90836044 pct_mod_tol_4_6 Percent abundance of taxa with Tolerance Values (TV) between 4 and 6 Producer Defined 18.92098718 96.65055824 pct_trichoptera Percent abundance of Trichoptera Producer Defined 0.237442922 45.0097371 pct_non_hydrop_trichoptera Percent abundance of Trichoptera minus Hydropsychidae family Producer Defined 2.53164557 89.28571429 pct_odonata Percent abundance of Odonata Producer Defined 0.0 2.56684492 pct_pisciforma Percent abundance of Pisciforma Producer Defined 0.566572238 37.96770382 pct_predator Percent abundance of taxa with the Functional Feeding Group (FFG) of Predator Producer Defined 0.073059361 8.659450458 pct_pterygota Percent abundance of Pterygota Subclass Producer Defined 19.24622154 99.58617332 pct_retreat_caddisfly Percent abundance of Trichoptera in Annulipalpia subfamily that build retreats Producer Defined 0 0 pct_scrape Percent abundance of taxa with the Functional Feeding Group (FFG) of scraper Producer Defined 0.127853881 20.05347594 pct_sprawl Percent abundance of taxa with the habit of sprawler Producer Defined 0.016663889 76.889229 pct_systellognatha Percent abundance of Systellognatha suborder Producer Defined 0.0 6.57785179 pct_tolerant_7_10 Percent abundance of taxa with Tolerance Value (TV) between 7 and 10 Producer Defined 0.034977265 76.8318347 pct_urban_intol Percent abundance of taxa deemed as intolerant of urbanization: "aeshnidae", "ameletidae", "asellidae", "athericidae", "baetidae", "brachycentridae", "caenidae", "calamoceratidae", "cambaridae", "capniidae", "ceratopogonidae", "chironomidae", "chloroperlidae", "cordulegastridae", "corduliidae", "corydalidae", "crangonyctidae", "elmidae", "ephemerellidae", "ephemeridae", "glossosomatidae", "gomphidae", "heptageniidae", "hydropsychidae", "hydroptilidae", "isonychiidae", "lepidostomatidae", "leptophlebiidae", "leuctridae", "libellulidae", "metretopodidae", "nemouridae", "odontoceridae", "peltoperlidae", "perlidae", "perlodidae", "philopotamidae", "phryganeidae", "polycentropodidae", "polymitarcyidae", "potamanthidae", "psephenidae", "pteronarcyidae", "rhyacophilidae", "sericostomatidae", "sialidae", "simuliidae", "stratiomyidae", "tabanidae", "taeniopterygidae", "tipulidae", "uenoidae", "viviparidae" Producer Defined 82.36493374 99.85388128 margalefs Margalefs Species Richness Index Producer Defined 0.868588964 4.342944819 pct_ept_rich Epheremoptera, Plecoptera and Trichoptera richness divided by all family richness Producer Defined 14.28571429 60.0 pct_ept_rich_no_tol Intolerant (Tolerance Value > than 6) Epheremoptera, Plecoptera and Trichoptera richness divided by all family richness Producer Defined 14.28571429 60.0 pielou Pielou's Evenness Index Producer Defined 0.456264981 0.886679215 rich_burrow Richness of taxa with habit category of burrower Producer Defined 1 4 rich_climb Richness of taxa with habit category of climber Producer Defined 0 3 rich_cling Richness of taxa with habit category of clinger Producer Defined 1 14 rich_collect Richness of taxa with functional feeding group (FFG) category of Collector-gatherer Producer Defined 4 12 rich_ephemeroptera Richness of Ephemeroptera Producer Defined 0 7 rich_ept Richness of Ephemeroptera, Plecoptera and Trichoptera Producer Defined 1 12 rich_filter Richness of taxa with functional feeding group (FFG) category of Filter-feeder Producer Defined 0 5 rich_gather Richness of taxa with functional feeding group (FFG) category of Collector-Gatherer Producer Defined 2 10 rich_intol Richness of taxa with Tolerance Value (TV) between 0 and 6 Producer Defined 0 9 rich_modtol Richness of taxa with Tolerance Value (TV) between 4 and 6 Producer Defined 3 11 rich_plecoptera Richness of Plecoptera Producer Defined 0 2 rich_predator Richness of taxa categorized as being predators in Functional Feeding Group (FFG) Producer Defined 0 4 rich_shred Richness of taxa categorized as being shredders in Functional Feeding Group (FFG) Producer Defined 0 4 rich_sprawl Richness of taxa categorized as being sprawlers in habit Producer Defined 0 3 rich_tol Richness of taxa with Tolerance Values (TV) between 7 and 10 Producer Defined 0 4 rich_trichoptera Richness of Trichoptera Producer Defined 0 7 GS ScienceBase U.S. Geological Survey mailing address

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Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P9H41UDY None 20231201 Sally Entrekin Virginia Tech, Department of Entomology Associate Professor mailing

Price Hall, Room 216A

Blacksburg VA 24061-0321 USA 540-231-5978 sallye@vt.edu FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999