Robin L. DeBruyne Jaquelyn M. Craig Gregory W. Kennedy Edward F. Roseman 20221205 tabular digital data Reston, VA U.S. Geological Survey Robin L. DeBruyne [(0000-0002-9232-7937), (U.S. Geological Survey, Great Lakes Science Center)] Jaquelyn M. Craig [(0000-0002-7601-8616), (U.S. Geological Survey, Great Lakes Science Center)] Gregory W. Kennedy [(0000-0003-1686-6960), (U.S. Geological Survey, Great Lakes Science Center)] Edward F. Roseman [(0000-0002-5315-9838), (U.S. Geological Survey, Great Lakes Science Center)] https://doi.org/10.5066/P9MMM8OT A long-term, multiseason, fish egg sampling program is conducted annually on the Detroit, Saint Clair, and Saint Marys rivers to identify where productive fish spawning habitat currently exists and evaluate habitat restoration projects. Egg mats were placed on the river bottom during the spring and fall at historic spawning areas, candidate fish spawning habitat restoration sites, and completed spawning habitat restoration sites throughout the rivers. Widespread evidence was found of lithophilic spawning by numerous native fish species, including walleye (Sander vitreus), lake whitefish (Coregonus clupeaformis), lake sturgeon (Acipenser fulvescens), suckers (Family: Catostomidae), and trout-perch (Percopsis omiscomaycus). The data describe the number, density, date of collection, and exact collection location of fish eggs collected in the St. Clair (2010-2022), Detroit (2005-2022), and St. Marys rivers (2018-2022). The data were collected as both general monitoring of lithophilic fish spawning throughout the rivers, and as pre- and post-assessment of spawning habitat restoration projects. 20050411 20220731 ground condition Complete Not planned St. Clair, Detroit, and St. Marys rivers, flowing between Michigan, USA and Ontario, Canada. -85.4297 -82.1338 46.8151 41.9431 ISO 19115 Topic Category biota inlandWaters National Agricultural Library Thesaurus fish reproduction aquatic habitat ecological restoration Sander vitreus Coregonus clupeaformis Acipenser fulvescens Catostomidae Percopsis Marine Realms Information Bank (MRIB) keywords habitat alteration fisheries biology Coastal and Marine Ecological Classification Standard Fish Spawning and Larval Aggregation Construction Reef None Walleye Lake Whitefish Lake Sturgeon Trout-perch USGS Metadata Identifier USGS:6307700cd34e3b967a8c0d8e USGS Thesaurus fishery resources habitat alteration and disturbance fish Geographic Names Information System (GNIS) Saint Clair River Detroit River Saint Marys River Michigan None lake sturgeon walleye trout-perch Catostomidae bony fishes Kingdom Animalia animals Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata chordates Subphylum Vertebrata vertebrates Infraphylum Gnathostomata Superclass Actinopterygii ray-finned fishes spiny rayed fishes Class Chondrostei paddlefishes sturgeons Order Acipenseriformes sturgeons spoonfishes paddlefishes Suborder Acipenseroidei Family Acipenseridae sturgeons Subfamily Acipenserinae Genus Acipenser sturgeons greater sturgeons Species Acipenser fulvescens lake sturgeon TSN: 161071 Class Teleostei Superorder Acanthopterygii Order Perciformes perch-like fishes Suborder Percoidei Family Percidae true perches perches Genus Sander pikeperches Species Sander vitreus walleye Superorder Paracanthopterygii Order Percopsiformes percopsiforms trout-perches Suborder Percopsoidei Family Percopsidae trout perches trout-perches Genus Percopsis trout-perches Species Percopsis omiscomaycus trout-perch TSN: 164409 Superorder Ostariophysi Order Cypriniformes minnows suckers Superfamily Cobitoidea Family Catostomidae suckers TSN: 163892 No data access constraints No data use constraints Robin L DeBruyne U.S. Geological Survey, MIDCONTINENT REGION Ecologist mailing address

1451 Green Rd

Ann Arbor MI 48105 US 734-994-3331 734-994-8780 rdebruyne@usgs.gov The data (or portions of the data) presented here has been supported through the years by the US Geological Survey, US Environmental Protection Agency's Great Lakes Restoration Initiative, and the US Army Corps of Engineers. Environment as of Metadata Creation: Microsoft Windows 7 Version 6.1 (Build 7601) Service Pack 1; Esri ArcGIS 10.4.1 (Build 5686) Service Pack N/A (Build N/A) Carson G. Prichard Jaquelyn M. Craig Edward F. Roseman Jason L. Fischer Bruce A. Manny Gregory W. Kennedy 2017 publication Scientific Investigations Report 2017-5003 n/a US Geological Survey https://doi.org/10.3133/sir20175003 Jason L. Fischer Jeremy J. Pritt Edward F. Roseman Carson G. Prichard Jaquelyn M. Craig Gregory W. Kennedy Bruce A. Manny 20180226 publication Transactions of the American Fisheries Society vol. 147, issue 1 n/a Wiley ppg. 79-93 https://doi.org/10.1002/tafs.10016 Robert D. Hunter Edward F. Roseman Nicholas M. Sard Robin L. DeBruyne Jinliang Wang Kim T. Scribner 20200320 publication Transactions of the American Fisheries Society vol. 149, issue 3 n/a Wiley ppg. 266-283 https://doi.org/10.1002/tafs.10225 Robert D. Hunter Edward F. Roseman Nick M. Sard Daniel B. Hayes Travis O. Brenden Robin L. DeBruyne Kim T. Scribner 20200320 publication North American Journal of Fisheries Management vol. 40, issue 2 n/a Wiley ppg. 307-319 https://doi.org/10.1002/nafm.10333 E. K. Bouckaert N. A. Auer E. F. Roseman J. Boase 20140924 publication Journal of Applied Ichthyology vol. 30, issue 6 n/a Wiley ppg. 1393-1401 https://doi.org/10.1111/jai.12603 E. F. Roseman B. Manny J. Boase M. Child G. Kennedy J. Craig K. Soper R. Drouin 20111205 publication Journal of Applied Ichthyology vol. 27 n/a Wiley ppg. 66-76 https://doi.org/10.1111/j.1439-0426.2011.01829.x Edward F. Roseman Gregory W. Kennedy James Boase Bruce A. Manny Thomas N. Todd Wendylee Stott 200701 publication Journal of Great Lakes Research vol. 33, issue 2 n/a Elsevier BV ppg. 397-406 https://doi.org/10.1016/S0380-1330(07)70064-3 Edward F. Roseman Eric Adams Robin L. DeBruyne Jason Gostiaux Hal Harrington Kevin Kapuscinski Ashley Moerke Christopher Olds 202010 publication Journal of Great Lakes Research vol. 46, issue 5 n/a Elsevier BV ppg. 1479-1484 https://doi.org/10.1016/j.jglr.2020.07.005 E. F. Roseman J. Boase G. Kennedy J. Craig K. Soper 20111205 publication Journal of Applied Ichthyology vol. 27 n/a Wiley ppg. 89-92 https://doi.org/10.1111/j.1439-0426.2011.01828.x Jaquelyn Craig Gregory Kennedy Edward Roseman 2017 dataset https://www.sciencebase.gov U.S. Geological Survey https://doi.org/10.5066/f7vd6wph Jaquelyn Craig Gregory W Kennedy Edward Roseman 2019 dataset https://www.sciencebase.gov U.S. Geological Survey https://doi.org/10.5066/p9vjmipo Wendylee Stott Robin DeBruyne Edward Roseman 20240729 publication International Journal of Limnology vol. 60 n/a EDP Sciences ppg. 10 https://doi.org/10.1051/limn/2024010 Calculations within the database were used to quality control data entry. Each record was double checked after data entry. Worked with a team of knowledgeable people to ensure all data are accurate. All values fall within expected ranges. Data entry was checked after entry to ensure no duplications/omissions occurred. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. All positions were marked using a GPS. All positions were marked with a GPS. E. F. Roseman J. Boase G. Kennedy J. Craig K. Soper 20111205 publication Journal of Applied Ichthyology vol. 27 n/a Wiley ppg. 89-92 https://doi.org/10.1111/j.1439-0426.2011.01828.x Digital and/or Hardcopy 20111205 publication date Roseman et al. 2011 Field methods Carson G. Prichard Jaquelyn M. Craig Edward F. Roseman Jason L. Fischer Bruce A. Manny Gregory W. Kennedy 20171231 publication n/a US Geological Survey https://doi.org/10.3133/sir20175003 Digital and/or Hardcopy 20171231 publication date Prichard et al. 2017 Egg Identification Methods Jenny L. Sutherland Bruce A. Manny Gregory Kennedy Edward F. Roseman Jeffrey Allen M. Glen Black 20140909 publication North American Journal of Aquaculture vol. 76, issue 4 n/a Wiley ppg. 391-398 https://doi.org/10.1080/15222055.2014.933751 Digital and/or Hardcopy 20140909 publication date Sutherland et al. 2014 Egg Hatch Methods Nancy A. Auer 19821231 publication Auer, Nancy A. “Identification of Larval Fishes of the Great Lakes Basin with Emphasis on the Lake Michigan Drainage.” Special Publication 82-3. Ann Arbor, MI: Great Lakes Fishery Commission, 1982. http://www.glfc.org/glfc-publications-reports.php?year=1982&issue=&keywords= Digital and/or Hardcopy 19821231 publication date Auer 1982 Methods Field collection for eggs. Egg mats were fished throughout the spring and fall spawning seasons, typically from mid-March through June or July, and again in late October through early to mid December in each year. Fish egg deposition was determined by placing furnace-filter egg mats on the river bottom using a small research vessel outfitted with a differential global positioning navigation system (GPS). Each egg mat consisted of a rectangle of furnace filter material wrapped around a metal frame and secured with four document clamps (see Roseman et al. 2011 for egg mat construction and field methods methods). Three to five such egg mats linked together, end to end, with lengths of braided nylon line formed a gang of egg mats (Roseman et al. 2011). At some MasterCode sites we placed 3 gangs of 4 mats each, resulting in 12 mats at that MasterCode site during those week(s) (see EggMatData.csv table). Each gang was anchored with a small trapnet anchor on the upstream end and a navy anchor on the downstream end. Both anchors were separated from the egg mats using a nylon braided line. The sample location was identified as a waypoint on the vessel’s GPS (see EggMatLocation.csv table). Our study typically left the egg mat gang in place in the river up to 16 weeks, returning once per week to retrieve the egg mats and inspect for eggs before redeploying for another week. Roseman et al. 2011 20050411 Robin L DeBruyne U.S. Geological Survey, MIDCONTINENT REGION Ecologist mailing address

1451 Green Rd

Ann Arbor MI 48105 US 734-994-3331 734-994-8780 rdebruyne@usgs.gov Egg identification. Egg mats were retrieved each week. All eggs were removed carefully with forceps, counted, identified, and then mats were redeployed. Identification was conducted on site with the naked eye and was based on size, color, transparency, oil globule position, and time of spawning (Prichard et al. 2017). Hand-held magnifying lenses were also used (and available) when necessary. Eggs identified and recorded belonged to walleye (Sander vitreus), lake whitefish (Coregonus clupeaformis), lake sturgeon (Acipenser fulvescens), suckers (Family: Catostomidae), and trout-perch (Percopsis omiscomaycus) fish species. Round goby (Neogobius melanostomus) and Morone spp. eggs were noted when present, but not counted due to their small size, high densities, and lengthy processing time. Mat number, body of water, and date were recorded for each egg retrieved at the time mats were pulled (see EggMatData.csv). Any juvenile or adult fish bycatch on the egg mats is noted in the Notes column in the EggMatData.csv table. To confirm egg identification, a subset of eggs was brought back to the USGS Great Lakes Science Center to be hatched following the methods in Sutherland et al. (2014) and larvae were identified according to Auer (1982). Prichard et al. 2017 Sutherland et al. 2014 Auer 1982 20220808 Robin L DeBruyne U.S. Geological Survey, MIDCONTINENT REGION Ecologist mailing address

1451 Green Rd

Ann Arbor MI 48105 US 734-994-3331 734-994-8780 rdebruyne@usgs.gov EggMatData.csv Comma Separated Value (CSV) file containing data from fish egg collections in the Detroit, St. Clair, and St. Marys rivers. Data include counts and densities by species and location. Producer Defined MasterCode This is the unique identifier for the sample site. It links to the specific latitude and longitude in the reference table entitled "egg mat location table through 2022 for data release" Producer Defined DTR represents Detroit River. SCR represents St. Clair River. SMR represents St. Marys River. The three-digit numeric portion is unique within each river. The whole alpha-numeric code is unique to a specific latitude and longitude. Week Week of sample collection. Producer Defined << empty cell >> Specific to sampling year 2020 when gear was not checked weekly due to field work restrictions during the Covid-19 pandemic. Gear was pulled and not assigned a week in database. Producer defined Sample sites were inspected weekly. Week 0 is generally the week in which the gear was set, and subsequent weeks the gear was inspected and reset. See Notes column for exceptions. Date Date of sample collection in MM/DD/YYYY format. Producer Defined Date of sample collection, except in the case of the first sample date within a sampling season (Week 0). In this case it is the date that sampling gear was set (noted in Notes column). Mat1_N The number of fish eggs collected on eggmat 1 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 9771 integer Mat2_N The number of fish eggs collected on eggmat 2 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 9142 integer Mat3_N The number of fish eggs collected on eggmat 3 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 3025 integer Mat4_N The number of fish eggs collected on eggmat 4 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 42 integer Mat5_N The number of fish eggs collected on eggmat 5 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 330 integer Mat6_N The number of fish eggs collected on eggmat 6 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 106 integer Mat7_N The number of fish eggs collected on eggmat 7 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 76 integer Mat8_N The number of fish eggs collected on eggmat 8 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 109 integer Mat9_N The number of fish eggs collected on eggmat 9 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 194 integer Mat10_N The number of fish eggs collected on eggmat 10 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 107 integer Mat11_N The number of fish eggs collected on eggmat 11 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 142 integer Mat12_N The number of fish eggs collected on eggmat 12 within the sampling gear. No data indicates that mat was lost or not included in the gear. Producer Defined << empty cell >> No Data Producer defined 0 170 integer Walleye_N The total number of walleye (Sander vitreus, TSN:650173) eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Producer Defined << empty cell >> No Data Producer defined 0 18825 integer SmallSucker_N The total number of smaller diameter sucker eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Possible species include Carpiodes cyprinus (TSN: 163917) and Carpiodes carpio (TSN:163919). Producer Defined << empty cell >> No Data Producer defined 0 770 integer LargeSucker_N The total number of larger diameter sucker eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Includes Moxostoma spp. (TSN:163927), Catostomus spp. (TSN:163893), and Hypentelium spp (TSN:163948). Producer Defined << empty cell >> No Data Producer defined 0 882 integer UnkSucker_N The total number of unidentified sucker (Catostomidae, TSN:163892) eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Producer Defined << empty cell >> No Data Producer defined 0 152 integer AllSuckers_N The sum of small, large, and unidentified sucker (Catostomidae, TSN:163892) eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Includes Carpiodes spp. (TSN:163916), Moxostoma spp. (TSN:163927), Catostomus spp. (TSN:163893), and Hypentelium spp. (TSN:163948) Producer Defined << empty cell >> No Data Producer defined 0 882 integer Troutperch_N The total number of trout-perch (Percopsis omiscomaycus, TSN:164409) eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Producer Defined << empty cell >> No Data Producer defined 0 322 integer LakeSturgeon_N The total number of lake sturgeon (Acipenser fulvescens, TSN:161071) eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Producer Defined << empty cell >> No Data Producer defined 0 576 integer LakeWhitefish_N The total number of lake whitefish (Coregonus clupeaformis, TSN:161941) eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Producer Defined << empty cell >> No Data Producer defined 0 280 integer Unidentified_N The total number of unidentified fish eggs collected on the sampling gear. These counts were determined from the collection of eggs pooled from the egg mats at that specific master code and date. Producer Defined << empty cell >> No Data Producer defined 0 248 integer BoatTemp_C Water temperature, in degrees Celsius, recorded with the boat's depth sounder. Producer Defined << empty cell >> No Data Producer defined -0.5 24.5 Degrees Celsius Secchi_m Secchi depth in meters. Producer Defined << empty cell >> No Data Producer defined 0.1 7.1 meters Notes Comments about the data, sampling gear deployment and retrieval, species by-catch, etc. Producer Defined << empty cell >> No Data Producer defined Comments about the data, sampling gear deployment and retrieval, species by-catch, etc. Mats_N Number of mats within the sampling gear at a sampling location. Producer Defined << empty cell >> No Data Producer defined 0 12 integer SampleArea_m2 Total area in square meters sampled by the sampling gear. Producer Defined << empty cell >> No Data Producer defined 0.0931 1.1172 meter squared TotalEggs_N Total number of fish eggs collected on the sampling gear. Producer Defined << empty cell >> No Data Producer defined 0 18825 integer TotalDensity_m2 Density of fish eggs collected on the sampling gear, measured as eggs per square meter. Producer Defined << empty cell >> No Data Producer defined 0 67400.64 eggs per square meter WalleyeDensity_m2 Density of walleye (Sander vitreus, TSN:650173) eggs collected on the sampling gear, measured as eggs per square meter. Producer Defined << empty cell >> No Data Producer defined 0 67400.64 eggs per square meter SmallSuckerDensity_m2 Density of smaller diameter sucker eggs collected on the sampling gear, measured as eggs per square meter. Possible species include Carpiodes cyprinus (TSN: 163917) and Carpiodes carpio (TSN:163919). Producer Defined << empty cell >> No Data Producer defined 0 2756.89 eggs per square meter LargeSuckerDensity_m2 Density of larger diameter sucker eggs collected on the sampling gear, measured as eggs per square meter. Includes Moxostoma spp. (TSN:163927), Catostomus spp. (TSN:163893), and Hypentelium spp. (TSN:163948) Producer Defined << empty cell >> No Data Producer defined 0.0 1736.48 eggs per square meter AllSuckersDensity_m2 Density of all species of sucker (Catostomidae, TSN:163892) eggs collected on the sampling gear, measured as eggs per square meter. Includes Carpiodes spp. (TSN:163916), Moxostoma spp. (TSN:163927), Catostomus spp. (TSN:163893), and Hypentelium spp. (TSN:163948) Producer Defined << empty cell >> No Data Producer defined 0 2756.89 eggs per square meter TroutperchDensity_m2 Density of trout-perch (Percopsis omiscomaycus, TSN:164409) eggs collected on the sampling gear, measured as eggs per square meter. Producer Defined << empty cell >> No Data Producer defined 0 1152.88 eggs per square meter LakeSturgeonDensity_m2 Density of lake sturgeon (Acipenser fulvescens, TSN:161071) eggs collected on the sampling gear, measured as eggs per square meter. Producer Defined << empty cell >> No Data Producer defined 0 2062.30 eggs per square meter LakeWhitefishDensity_m2 Density of lake whitefish (Coregonus clupeaformis, TSN:161941) eggs collected on the sampling gear, measured as eggs per square meter. Producer Defined << empty cell >> No Data Producer defined 0 1002.51 eggs per square meter UnidentifiedDensity_m2 Density of unidentified species of fish eggs collected on the sampling gear, measured as eggs per square meter. Producer Defined << empty cell >> No Data Producer defined 0.0 887.93 eggs per square meter Year Sampling year. Producer Defined 2005 2022 year Month Numeric code for month, March = 3 through December = 12. Producer Defined 3 Month of March Producer defined 4 Month of April Producer defined 5 Month of May Producer defined 6 Month of June Producer defined 7 Month of July Producer defined 8 Month of August Producer defined 10 Month of October Producer defined 11 Month of November Producer defined 12 Month of December Producer defined Season Sampling season. Producer Defined << empty cell >> No Data Producer defined Spring Samples collected from March 1 to July 31. Producer defined Fall Samples collected from October 1 to December 31. Producer defined EggMatLocation.csv Comma Separated Value (CSV) file containing location information for fish egg collections in the Detroit, St. Clair, and St. Marys rivers. Producer Defined MasterCode This is the unique identifier for the sample sites. Producer Defined DTR represents Detroit River. SCR represents St. Clair River. SMR represents St. Marys River. The three-digit numeric portion is unique within each river. The whole alpha-numeric code is unique to a specific latitude and longitude. Waterbody Samples were collected from three waterbodies: the St. Clair River, Detroit River, and St. Marys River. Producer Defined Detroit River The Detroit River flows from Lake St. Clair to Lake Erie, between Michigan, USA, and Ontario, Canada. Producer defined St. Clair River The St. Clair River flows from Lake Huron to Lake St. Clair, between Michigan, USA, and Ontario, Canada. Producer defined St. Marys River The St. Marys River flows from Lake Superior to Lake Huron, between Michigan, USA, and Ontario, Canada Producer defined Latitude The latitude for the MasterCode. Geographic projection, decimal degrees, WGS84 datum. Producer Defined 42.066829999999996 46.50978 decimal degrees Longitude Longitude for the MasterCode. Geographic projection, decimal degrees, WGS84 datum. Producer Defined -84.37536999999999 -82.41283 decimal degrees Depth_m Depth was measured using the GPS/depth sounder system for reference purposes only, to ensure gear construction dimensions were appropriate for the water depth, and to verify sample site location was correct when making repeat sample collections at that MasterCode site. The depth entry was taken during the first sampling event at that specific location and is not standardized to any charts, low water datum, etc. Missing depth data represents that depth readings were not collected at the MasterCode location. Depth is measured in meters. Producer Defined << empty cell >> No data collected. Producer defined 0.7 24.5 meters The entity and attribute information provided here describes the tabular data associated with the data set. Please review the detailed descriptions that are provided (the individual attribute descriptions) for information on the values that appear as fields/table entries of the data set. The entity and attribute information was generated by the individual and/or agency identified as the originator of the data set. Please review the rest of the metadata record for additional details and information. U.S. Geological Survey GS ScienceBase mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P9MMM8OT none. 20250124 Great Lakes Science Center mailing and physical

1451 Green Road

Ann Arbor MI 48105 US 734-994-3331 GS_ASK_GLSC@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999