Kraus, Johanna M. Pribil, Michael J. Rutherford, Danny L. Stricker, Craig A. McGee, Ben N. Todd, Andrew S. Holloway, JoAnn M. 20220414 Digital tabular data Reston, VA U.S. Geological Survey Authors Open Researcher and Contributor Id (ORCID) are as follows: Johanna M. Kraus:0000-0002-9513-4129, Michael J. Pribil:0000-0003-4859-8673, Danny L. Rutherford:0000-0003-1013-8006, Craig A.Stricker: 0000-0002-5031-9437, Ben N. McGee:0000-0001-8798-0037, JoAnn M. Holloway:0000-0003-3603-7668 https://doi.org/10.5066/P9P43XBX Johanna M. Kraus JoAnn M. Holloway Michael J. Pribil Ben N. McGee Craig A. Stricker Danny L. Rutherford Andrew S. Todd 20220411 publication n/a Wiley https://doi.org/10.1002/etc.5342 This data release includes sampling location data; field-collected stream attribute data; laboratory-analyzed chemistry concentrations (total mercury, methyl mercury) and isotopic composition (total mercury, carbon, and nitrogen) for stream biota (seston, periphyton, benthic insects, emerging adult insects, riparian spiders, and fish); density, body length, and taxonomic information for benthic insects; and density, biomass, and taxonomic information for emerging adult aquatic insects for biota sampled from stream reaches up and downstream of an historical mercury mine site. Sampling took place during summer low-flow conditions in 2015 and 2016. Stream reaches were located on U.S. Forest Service land near the Cinnabar Mine Site in the vicinity of Yellow Pine, ID, USA. Version 2.0 includes an additional table which describes subsampling of the Hess samples during processing. All data on benthic macroinvertebrates derived from Hess samples should be interpreted using this information about proportion of sample processed. These data were collected to test hypotheses about the ecological effects of mercury mining on downstream linked aquatic and riparian food webs. They were collected as part of a larger effort to understand chemical and physical processes linked to downstream effects of mining in this watershed. Taxonomy of insects is provided to class in the metadata. Complete taxonomy of sampled insects can be found in Taxonomy.sgml 20150723 20160803 ground condition Complete None planned Boundary box for sampling locations -115.3245 -115.2914 44.95361 44.9386 USGS Thesaurus food web mine drainage mercury contamination macroinvertebrates riparian ecosystems ISO 19115 Topic Category biota National Agricultural Library Thesaurus aquatic insects biomagnification spiders None aquatic-terrestrial linkages riparian spiders Nez Perce Tribe USGS Metadata Identifier USGS:621e2f68d34ee0c6b389a7de Geographic Names Information System Idaho sugar creek Yellow Pine USGS Biocomplexity Thesaurus Arachnids Fishes Insects Integrated Taxonomic Information System (ITIS) 2022 ONLINE_REFERENCE Washington, D.C. Integrated Taxonomic Information System (ITIS) http://itis.gov Dondale, C.D Redner, J.H., 2003 Digital tabular data Unknown NRC Research Press McAlpine, J.F. Peterson, B.V. Shewell, G.E. Teskey, H.J. Vockeroth, J.R. Wood, D.M. 1981 Digital tabular data Quebec, Canada Canadian Government Publishing Centre Merritt, R.W. Cummins, K.W. Berg, M.B. 2008 Digital tabular data Duguque, IA. USA. Kendall Hunt Publishing Triplehorn, C.A. Johnson, N.F. 2005 Digital tabular data United States Thomson Brooks/Cole Ubick, D. Paquin, P. Cushing, P.E. Roth, V.D. 2005 Digital tabular data Unknown American Arachnological Society Johanna M Kraus U.S. Geological Survey, Columbia Environmental Research Center Ecologist mailing and physical

4200 New Haven Road

Columbia MO 65201 US 573-875-5399 jkraus@usgs.gov expert advice;;identification keys Identification keys were used to perform taxonomic identification. Benthic aquatic insects were identified by Timberline Aquatics. Other identifications were done in house by Ben McGee. All benthic insects were identified to the level of family, genus, or species. All adult aquatic insects were identified to the level of family. All spiders were identified to the level of family, or genus. All fish were identified to speceis. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Actinopterygii Class Teleostei Superorder Protacanthopterygii Order Salmoniformes Family Salmonidae Subfamily Salmoninae Genus Salvelinus Species Salvelinus confluentus bull trout Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Hexapoda Class Insecta Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Chelicerata Class Euchelicerata Subclass Arachnida Order Araneae Family Tetragnathidae longjawed orbweavers None. Please see 'Distribution Info' for details. These data are marked with a Creative Common CC0 1.0 Universal License. These data are in the public domain and do not have any use constraints. It is requested that the authors be cited for any subsequent publications that reference this dataset. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Johanna M Kraus U.S. Geological Survey, Columbia Environmental Research Center Ecologist mailing and physical

4200 New Haven Road

Columbia MO 65201 US 573-875-5399 jkraus@usgs.gov Holloway, J.M. Pribil, M.J., McCleskey, R.B., Rutherford, D.L., Baldwin, A.K., DeWild, J.F., Breitmeyer, S.E., 2018 OTHER Reston, VA U.S. Geological Survey https://doi.org/10.5066/P9WC9J0M Delphine Foucher Holger Hintelmann 20060321 publication Analytical and Bioanalytical Chemistry vol. 384, issue 7-8 n/a Springer Science and Business Media LLC ppg. 1470-1478 https://doi.org/10.1007/s00216-006-0373-x Instrument calibrations, visual review of all data during data entry, spot-checking data, summary statistics on data, quality control procedures by laboratory were conducted to ensure accuracty of data. Review of original field datasheets, review of database construction notes and documentation, visual plotting of the data, review of chemical datasets with analytical chemists. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. GPS readings were taken multiple times at a site and were used to locate sites in both years of the study. Locations were confirmed with satellite images and previous maps of the sites produced by other scientists. Elevation is an approximate metric and matched with the known/mapped elevation range for the location. both Sampling was conducted within two 50-m reaches at each stream site (N = 5) during summer base flow on July 23-28, 2015 and August 3, 2016. Seston, biofilm, larval aquatic insects and spiders were collected from the downstream reach, while adult aquatic insect emergence were collected from the undisturbed upstream reach. Additional riparian spiders were collected from the same sites in 2016. Water, sediment and fish, mainly native resident bull trout (Salvelinus confluentus), were also collected as part of an extended study of the area in 2015-16. Field water chemistry parameters, including pH, dissolved oxygen, and specific conductance, were measured at each site during 2015 stream ecology sampling. Stream geomorphology and riparian vegetation metrics were also measured within each reach at that time. Seston, periphyton, larval aquatic insects, adult aquatic insects, spiders and fish were prepared for chemical analysis. Samples were analyzed for mercury in two different laboratories. Samples were analyzed for total mercury concentration and isotopic composition in the High-Resolution Laboratory at the USGS (Denver, Colorado). A subset of biota and seston samples, some splits and some previously unanalyzed, were analyzed for total mercury and methyl mercury concentrations in the USGS Mercury Laboratory in Wisconsin. Light stable isotope analysis was completed on sample splits. Holloway, J.M. and others. 2018 Tabular Reston, VA U.S. Geological Survey https://doi.org/10.5066/P9WC9J0M Tabular 20150626 20170125 Current at time of publication Geochem Dissolved concentrations of arsenic in stream water were used to bolster the argument that patterns seen in our data were likely due to mercury contamination alone. Study Area: Our study took place near the Cinnabar mine site in the East Fork South Fork Salmon River (EFSFSR) watershed, an area of 528 km2 in the Salmon River Mountains in central Idaho, USA. Historical mining for gold, antimony, tungsten, and Hg in the EFSFSR watershed at the Cinnabar and Stibnite mine sites resulted in elevated Hg and arsenic (As) concentrations in sediments and water in Cinnabar Creek and in Sugar Creek below the confluence with Cinnabar Creek relative to unmined background concentrations. Stream reaches in this study include background sites Cane Creek above the confluence with Sugar Creek (site ID021) and Sugar Creek directly upstream of the confluence with Cane Creek and above the confluence with Cinnabar Creek (ID020, Sugar Creek Up, also referred to as "upstream comparison site"). Mining-impacted sites include Cinnabar Creek upstream of the confluence with Sugar Creek (ID019, ~4.3 km downstream of the mine site), Sugar Creek ~1.4 km downstream of Cinnabar Creek (ID018, Sugar Creek Mid), and Sugar Creek ~3.3 km downstream of Cinnabar Creek (ID010, Sugar Creek Down). 2016 Field Sampling: Field sampling focused on characterizing effects of mine drainage on 1) benthic and adult aquatic insect density, biomass, and species diversity and 2) Hg accumulation and flux through the aquatic-riparian food web. Mercury metrics included total Hg (THg) and MeHg concentrations in sediment, organic matter, and biota and the total mass of Hg m-2 (or m-2 d-1) in the aquatic insect samples (or subsamples for some Hess samples). Sampling was conducted within two 50-m reaches at each stream site (N = 5) during summer base flow on July 23-28, 2015 and August 3, 2016. Seston, biofilm, larval aquatic insects and spiders were collected from the downstream reach, while adult aquatic insect emergence were collected from the undisturbed upstream reach. Additional riparian spiders were collected from the same sites in 2016. Water, sediment and fish, mainly native resident bull trout (Salvelinus confluentus), were also collected as part of an extended study of the area in 2015-16. Field water chemistry parameters, including pH, dissolved oxygen, and specific conductance, were measured ~10 cm below the surface of the water using a YSI Professional Plus Multiparameter Water Meter at each site during 2015 stream ecology sampling. Stream geomorphology was defined by stream width and areas of laminar (i.e., pools) and non-laminar (i.e., riffles) flow. Wetted width was measured every 10-meters. Riparian vegetation was characterized by canopy cover measured in the middle of the stream, volume of vegetation overhanging the stream up to 2 meters above the water surface, and the number of trees and shrubs on each bank within 1 meter of the stream. Canopy cover was estimated using a densiometer that was held chest height facing in each of four directions (upstream, right bank, downstream, left bank) every 10 meters. Numbers of trees and shrubs were counted within each 10 meter segment. Volume of vegetation overhanging the stream was estimated by eye as the product of length and width of vegetation above water within each 10 meter segment. 2016 Ecological Sample Collection: Benthic aquatic insects were sampled for biomass, density, and diversity from the stream bottom using a Hess sampler (Wildco Hess Stream Bottom Sampler, 243 micrometer net, bottom open area: 0.086 square meters). The sampler was placed opportunistically in areas where an effective seal against invertebrate escape could be obtained along the stream bottom. All hand-sized and larger cobbles were scrubbed and checked for invertebrates before removing them from the sampler. Finer substrate was thoroughly disturbed with a gardening claw, and buried cobbles were dislodged. Samples were not elutriated in the field but transferred directly to a 532-ml polyethylene plastic bag (Whirl-Pak sample bags) and preserved in 80% ethanol. Three Hess samples were collected each stream site within a 50 meter reach. Habitats targeted for sampling were characterized by coarse, stable substrate, intermediate depths, and a moderate current (i.e., low-gradient riffles or runs). Water depth at the sample location was recorded. Emerging adult aquatic insects were collected for biomass, density, and diversity, using floating emergence traps (bottom open area, 0.36 square meters). Briefly, mesh traps were built on a polyvinyl chloride (PVC) pyramid-shaped frame floating on polyethylene foam and anchored by a nylon cord tied to rebar. High density polyethylene (HDPE) collection bottles loosely filled with mesh were attached the side of the trap near the top. Traps were deployed over pools or areas of slow-moving water. After four days, bottles were capped, and trap contents were hand aspirated. Samples were placed on ice in field then frozen in a conventional freezer. Five traps total were deployed per stream site with one trap placed within each of the 10 meter stretches available in the 50 meter reach. Location and water depth under the traps were recorded. 2016 Chemical Sample Collection: Briefly, stream sediment was collected into pre-cleaned glass jars and frozen on dry ice for transport to the U.S. Geological Survey (USGS) Mercury Laboratories in Wisconsin, USA. An additional split of sediment was shipped frozen to USGS laboratories in Denver, CO, where the material was air-dried and sieved to less than 2 mm for full chemical analyses. Seston, i.e., particulate matter in the water column, was collected using a plankton net fitted with a dolphin bucket (Wildco Dolphin Plankton Bucket-1000mL, 241 micrometer mesh) and set in the water column for 1.5 to 4 hours as a modified drift net. Material collected in the dolphin bucket was filtered through a plastic 250-micrometer sieve to remove large course particulate organic matter (CPOM), and then through a 30-micrometer sieve to remove excess water. Periphyton and associated biofilm were collected by scrubbing 4 - 5 cobble sized rocks from three locations within each stream site. Rocks were chosen that had abundant periphyton and no filamentous algae. Macroinvertebrates and Nostoc cyanobacterial mats were removed from the rocks before scrubbing. Periphyton and attached particulates were scrubbed from the rocks using a course brush and Teflon scraper. Larval aquatic insects were collected for chemical analysis by kicking the substrate and capturing drifting insects in a net downstream (i.e., kick-netting). Kick-net samples were sorted in the field. Abundant large taxa and common taxa found at multiple sites were targeted. Emerging adult aquatic insects were collected for chemical analysis using emergence traps floating on the surface of pools over a 4-day period (same samples as for density, biomass, and diversity). To avoid sample contamination for chemical analysis, no preservatives were used in the collection bottles associated with the kick samples or emergence traps. Fish were collected by a two-person quantitative sampling crew using a Smith-Root LR24 backpack electrofishing unit. One reach at each site was surveyed using two-pass removal (quantitative depletion) methods. All salmonids were measured for length (mm) and salmonids greater than 75mm were weighed (g) using spring drop scales. Up to ten bull trout collected at each site were harvested for Hg tissue analysis. Fish tissue sampling efforts were conducted in accordance with U.S. Fish and Wildlife Service Endangered Species Permit Office, Native Threatened Species Recovery Permit TE-89855B-0 and the U.S. Department of Commerce, National Oceanic and Atmospheric Administration, National Marine Fisheries Service Guidelines for Electrofishing Waters Containing Salmonids Listed Under the Endangered Species Act, June 2000. Riparian web-building spiders were collected off terrestrial vegetation, mainly Alnus and Salix shrubs, within 1-m of the stream channel using beat sheets or hand collection methods. Spiders in the family Tetragnathidae (Araneae) were targeted because their diets have been previously found to consist largely of adult aquatic insects. Leaves from shrubs from which spiders were sampled were also hand-collected to rule out a terrestrial source of Hg for riparian spiders (mediated by terrestrial insect herbivores). All samples collected for chemical analysis were collected using clean techniques for metals (i.e., nitrile gloves rinsed with deionized water, fiber forceps, polyethylene vials) and stored on ice in the field and frozen in a conventional freezer until sorting and analysis. Additionally, all fish processing equipment was sterilized with 20% bleach solution and triple rinsed with DI water between each monitoring location. 2016 Taxonomy: Benthic aquatic insect samples were sorted and identified to the lowest practical taxonomic level (mainly genus) using the whole or subsample of each Hess sample (if sample contained more than 300 individuals) by Timberline Aquatics, Inc. (Fort Collins, CO, USA; https://www.timberlineaquatics.com/). Body length was measured for each specimen to the nearest millimeter. Biomass for benthic insect taxa in the Hess sample (or subsample) was estimated using length-mass regressions. Emerged adult aquatic insects were sorted and identified to taxonomic family using McAlpine et al. (1981), Ward et al. (2002), Merritt et al. (2008). After sorting, emergence samples were freeze-dried and weighed separately by family and trap using a 6-point microbalance (Sartorius MSE6.6S). Riparian spiders were identified to taxonomic family, freeze-dried and weighed separately by individual using a 6-point microbalance 2016 Chemical Analyses: Seston, periphyton, larval aquatic insects, adult aquatic insects and spiders were freeze-dried and prepared for chemical analysis. Fish samples were stored at approximately -60 degrees Celsius until moved to a -16 degrees Celsius freezer during sample preparation events. Fish were laterally filleted to remove muscle tissue along the anterior dorsal using a presterilized stainless steel surgical blade. Skin was removed and muscle tissue placed into a labeled metal free polycarbonate tube. Once processed, tissue sample tubes were again stored at approximately -60 degrees Celsius until analyzed. For analysis of THg and MeHg concentrations, and THg stable isotopic composition (delta202Hgtotal only reported here), samples were shipped directly to the laboratories (section 2.4.2) after drying and removing a split for carbon and nitrogen isotope analysis (delta13C, delta15N). Samples were prepared for carbon and nitrogen stable isotope analysis by using stainless steel instruments to fill a tin capsule with approximately 1 mg of dried, homogenized material. Samples were prioritized for analysis based on study questions and sample mass. Animal samples were composited by taxa (insects) or size class (spiders) before analysis. 2016 Total Mercury and Mercury Isotopic Analysis: Samples were analyzed for Hg in two different laboratories. Samples were analyzed for THg concentration and isotopic composition in the High-Resolution Laboratory at the USGS (Denver, Colorado) using a Nu Instruments HR multi-collector inductively coupled plasma mass spectrometer (MC-ICP-MS). Briefly, all samples were digested in concentrated HNO3 and HCl (9:1). In addition, 1 mL of concentrated H2O2 was added to aid in the digestion of organic matter. Samples were heated on a hot plate at 80 degrees Celsius for 4 to 6 h and diluted with 18 milliohm water. For isotopic analyses, Hg was introduced into the MC-ICP-MS using a CETAC HGX-200 cold vapor (CV) generation system. Argon carrier gas was introduced into the CV system at a flow rate of 55-65 mL/min and a 3% w/v solution of SnCl2 was used as the reductant. Thallium (NIST SRM 997) was introduced to the CV system sample flow via a desolvating nebulizer to correct for mass bias. All samples are reported in parts per thousand (delta notation, relative to National Institute of Standards and Technology (NIST) SRM 3131. The UM-Almaden secondary isotopic reference standard (NIST SRM 8610) resulted in an average delta202Hg of -0.56 plus or minus 0.08 parts per thousand (mean plus or minus 2 stdev) and delta199Hg of -0.02 plus or minus 0.08 parts per thousand (n=50). Additionally, a sediment reference material (NIST SRM 1944) was prepared multiple times and resulted in average delta202Hg of -0.53 plus or minus 0.04 parts per thousand and delta199Hg of -0.00 plus or minus 0.08 parts per thousand (n=5) within error of previously reported values. Calibration curves were created by using varying concentrations of NIST SRM 3133 (ranging from 0 to 4 ng per g; using THg response in volts) and checked throughout the sampling period using a 2.5 ng g-1 SRM 3133 bracketing standard. The 2.5 ng g-1 SRM 3133 solution resulted in an average concentration of 2.47 plus or minus 0.12 ng per g (2SD) over a four-month period. All samples were dried before analysis except for fish tissue. Total Hg concentrations in fish tissue were converted from wet wet (ww) to dry weight (dw) concentrations by dividing by 0.25 (THg concentration in wet fish tissue was empirically determined to be 24 plus or minus 2% [mean, stdev] of dw concentrations for n = 4 fish). 2016 Methylmercury and Total Mercury Analysis: A subset of biota and seston samples, some splits and some previously unanalyzed, were analyzed for THg and MeHg concentrations in the USGS Mercury Laboratory in Wisconsin. Briefly, samples were analyzed for THg and MeHg by atomic florescence using a Brooks Rand MERX-T Automated Total Mercury System. Data review suggested that two values for fish (ID018 and ID020) had been mislabeled, so values were switched. One spider value (for spider greater than 11mg) was removed as an outlier due to the unusually low MeHg% (6.5%) that was an order of magnitude lower than all other spider samples, suggesting contamination issues. All samples were dried before analysis. 2016 Light Stable Isotope Analysis: Sample splits (~1 mg dry mass) for stable isotope analyses were transferred to 4 x 6 mm tin capsules (Costech Analytical, Inc.) using an analytical balance, sealed, and stored in 96-well titer plates for analysis by continuous flow isotope ratio analysis using an Micromass Optima mass spectrometer at the USGS Stable Isotope Laboratory located in Denver, CO. Isotopic data expressed in per mil notation (‰) were normalized to the respective scales using USGS 40 (-4.52 per mil for d15N, -26.24 per mil for d13C), USGS 41 (47.57 per mil for d15N, 37.76 per mil for d13C) and USGS 41a (47.55 per mil for d15N, 36.55 per mil for d13C). Analytical precision and replicate sample analyses were generally +/- 0.2 per mil for both isotopes with some exceptions. 2016 SiteCharacteristics Text(txt)file containing information on physical and chemical characteristics of stream site where sampling occurred measured once per stream site. Producer Defined Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Text Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Date A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which the survey began. Producer Defined 20150723 20150725 Date Time Time of day when survey began. Expressed in 24 hour clock, HH:MM Producer Defined 11:24 17:33 Hours Metric A literal value representing the element measured. Text Producer Defined air_temp Air temperature, reported in degrees Celsius Producer defined conductivity A measure of the ability of water to pass an electrical current, reported in microsiemens per centimeter. Producer defined depth Depth of wetted stream channel at water sampling location, reported in meters Producer defined DO_concentration Concentration of gaseous oxygen in water or water solutions, unfiltered, reported as milligrams per liter. Producer defined DO_percent The percentage of dissolved oxygen concentration relative to that when completely saturated at the temperature of the measurement depth Producer defined elevation Distance above sea level in meters, using GPS model GPSMAP62STC Producer defined latitude Geographic coordinate that specifies the north–south position of a point on the Earth's surface. Reported in decimal degrees, WGS84, using GPS model GPSMAP62STC Producer defined longitude Geographic coordinate that specifies the east-west position of a point on the Earth's surface. Reported in decimal degrees, WGS84, using GPS model GPSMAP62STC Producer defined pH_1 The first measurement of logarithm of the reciprocal hydrogen ion concentration in atoms per liter or acidity of sample water (liquid) Producer defined pH_2 The second measurement of logarithm of the reciprocal hydrogen ion concentration in atoms per liter or acidity of sample water (liquid) Producer defined pH_3 The third measurement of logarithm of the reciprocal hydrogen ion concentration in atoms per liter or acidity of sample water (liquid) Producer defined pH_4 The fourth measurement of logarithm of the reciprocal hydrogen ion concentration in atoms per liter or acidity of sample water (liquid) Producer defined SPC Reciprocal of the resistance in ohms measured between opposite faces of a centimeter cube of an aqueous solution at a specified temperature, 25° Celsius, reported as microsiemens per centimeter Producer defined stream_width Width of the wetted stream channel at water sampling location, reported in meters Producer defined water_temp water temperature of the stream at the sampling location, reported in degrees Celsius Producer defined Result The analytical result for the METRIC measured. Numeric Producer Defined -115.32 1949.71 see UNITS attribute Units The standard unit of measure for the RESULT. Text National Institute of Standards and Technology (NIST) unitless m Producer defined The standard unit of measure for the RESULT. Text. https://www.nist.gov/pml/weights-and-measures/metric-si/si-units HessTrapInformation Text (txt) file containing information on characteristics of Hess trap sampling including depth of water at trap location. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples were collected. Producer Defined 2015 2015 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Trap_No Numeric identifier for the trap within a stream reach Producer Defined Numeric identifier for the trap within a stream reach. This is a key attribute that ties to other data tables. Trap_Location Position of trap within 50 meter reach sampled for spiders and benthic insects, reported in meters. "0" indicates upstream most limit of reach and "50" indicates downstream most limit of reach, in meters. Producer Defined 13 38 meters Trap_Depth Depth of water in location of hess trap reported in meters. Producer Defined 0.1 0.3 meters HessTrapCount Text (txt) file containing information for benthic aquatic insects captured in sample or subsampled (if sample contained more than 300 individuals) of Hess sampler deployed on bottom of stream including count and total body length of insects captured in trap grouped by taxa. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples were collected. Producer Defined 2015 2015 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Trap_No Numeric identifier for the trap within a stream reach Producer Defined Numeric identifier for the trap within a stream reach. This is a key attribute that ties to other data tables. Taxa A literal value representing the lowest level of taxonomic hierarchy to which the sample organism was identified. In Linnaean classification system called the taxonomic hierarchy, which today has eight ranks of traits from general to specific: domain, kingdom, phylum, class, order, family, genus, and species. Text. Producer Defined The lowest level of taxonomic hierarchy to which the sample organism was identified. In Linnaean classification system called the taxonomic hierarchy, which today has eight ranks of traits from general to specific: domain, kingdom, phylum, class, order, family, genus, and species. Chelifera/Neoplasta Specimen is a true fly belonging to genera Cheliferaone or Neoplasta Producer defined Cricotopus/Orthocladius Specimen is a true fly belonging to genera Cricotopus or Orthocladius Producer defined Microspectra/Tanytarsus Specimen is a true fly belonging to genera Microspectra or Tanytarsus Producer defined Rhyacophila coloradensis group Specimen is a caddisfly belonging to the Rhyacophila coloradensis species complex of caddisfly Producer defined Rhyacophila siberica group Specimen is a caddisfly belonging to the Rhyacophila siberica species complex of caddisfly Producer defined Zapada oregonensis group Specimen is a stonefly belonging to the Zapada oregonensis species complex of stonefly Producer defined Life_Stage A literal value representing the stage of the insect life cycle to which the sample organism had developed. Text Producer Defined larvae An active immature stage of insect life cycle. The larva is a specialized feeding stage that looks very different from the adult Producer defined pupae A resting stage of insect life cycle. The pupal stage is a transition stage, when the larva transforms into the adult. Wings, if present, develop internally within a pupa. The pupa molts to the adult form. Producer defined adult The mature stage of insect life cycle. Producer defined Count An enumeration of the benthic aquatic insects in the sample or subsample. Integer Producer Defined 1 212 observed individuals Sum_Length Summed body lengths of all benthic aquatic insects in sample (or subsample) measured to the nearest millimeter. Producer Defined 2 504 millimeters HessLabSubsample Text (txt) file containing information regarding processing of benthic aquatic insects captured in Hess sampler deployed on bottom of stream including proportion of total sample enumerated and measured. Producer defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples were collected. Producer defined 2015 2015 YYYY Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Text. Producer defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Sample_Type A literal value for the type of organism included in the sample Producer defined Benthic Aquatic Insect Aquatic insects captured underwater in aquatic swimming stages of life history Producer defined Trap_No Numeric identifier for the trap within a stream reach Producer defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Grid_Sample Number of square grids randomly sampled during identification of macroinvertebrates collected from Hess sample. Producer defined 7 24 count Grid_Total Number of square grids total available for sampling during identification of macroinvertebrates collected from Hess sample Producer defined 24 24 count Subsample Proportion of square grids randomly sampled during identification of macroinvertebrates collected from Hess sample. This value is calculated by dividing "grid_sample" by "grid_total". Producer defined 0.29 1.0 proportion ReachCharacteristics Text (txt) file containing information on physical and biological characteristics of stream site where sampling occurred measured across multiple reaches (on an area basis) along the stream within a site. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples were collected. Producer Defined 2015 2015 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Text Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Reach_Type An alpha code that identifies the major taxonomic group sampled within the reach Producer Defined EM Reach sampled for emerging aquatic insects. Reach was always upstream of reach sampled for spiders and benthic insects. Producer defined SP Reach sampled for riparian spiders and benthic aquatic insects. Reach was always directly downstream of reach sampled for emerging aquatic insects. Producer defined Reach_Location A numeric identifier for the 10 meter segment within a 50 meter reach where samples where collected. "0" indicates upstream most limit of reach and "50" indicates downstream most limit of reach, in meters. Producer Defined 2 Segment sampled was located at 10 to 20 meters Producer defined 1 Segment sampled was located at 0 to 10 meters Producer defined 3 Segment sampled was located at 20 to 30 meters Producer defined 4 Segment sampled was located at 30 to 40 meters Producer defined 5 Segment sampled was located at 40 to 50 meters Producer defined Metric A literal value representing the element measured. Text Producer Defined pool_area Total area of laminar flow within the active channel, in meters squared. Producer defined pools Count of distinct areas of laminar flow within the active channel Producer defined riffle_area Total area of highly non-laminar flow within the active channel, in meters squared. Producer defined riffles Count of distinct areas of highly non-laminar flow within the active channel. Producer defined shrubs Count of shrubs along both banks of the stream within 1 meter of the active channel. Shrubs have multiple branching trunks. Producer defined vegetation Estimated volume of living and dead vegetation over active stream channel up to 2 meters above the water, in meters cubed. Producer defined treesGT2m Count of trees greater than 2 meters tall along both banks of the stream within 1 meter of the active channel. Trees have 1 main trunk. Producer defined treesLT2m Count of trees less than 2 meters tall along both banks of the stream within 1 meter of the active channel. Trees have 1 main trunk. Producer defined Result The analytical result for the METRIC measured. Numeric Producer Defined 0.0 40.0 see UNITS attribute Units The standard unit of measure for the RESULT. Text National Institute of Standards and Technology (NIST) count Unit used to express the enumeration of the METRIC analyzed Producer defined The standard unit of measure for the RESULT. https://www.nist.gov/pml/weights-and-measures/metric-si/si-units PointCharacteristics.txt Text (txt) file containing information on physical and biological characteristics of stream site where sampling occurred measured at multiple points along the stream within a site. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples were collected. Producer Defined 2015 2015 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Text Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Reach_Type An alpha code that identifies the major taxonomic group sampled within the reach Producer Defined EM Reach sampled for emerging aquatic insects. Reach was always upstream of reach sampled for spiders and benthic insects. Producer defined SP Reach sampled for riparian spiders and benthic aquatic insects. Reach was always directly downstream of reach sampled for emerging aquatic insects. Producer defined Point_Location Position in reach where measurement was taken, in meters. Upstream limit of reach is indicated by "0", and downstream limit is indicated by "50". Producer Defined 0 50 meters Metric A literal value representing the element measured. Text Producer Defined densiometer_downstream Count of densiometer squares covered by shade of canopy trees while observer faces downstream. Producer defined densiometer_leftbank Count of densiometer squares covered by shade of canopy trees while observer faces left bank. Producer defined densiometer_rightbank Count of densiometer squares covered by shade of canopy trees while observer faces right bank. Producer defined densiometer_upstream Count of densiometer squares covered by shade of canopy trees while observer faces upstream. Producer defined stream_width Width of active stream channel, in meters. The active channel is defined as a portion of the channel that is somewhat lower than bankfull, the portion of the channel commonly wetted during and above base flows. The ordinary high water mark is sometimes given as the elevation defining the active channel. Producer defined Result The analytical result for the METRIC measured. Numeric Producer Defined 1.3 96.0 see UNITS attribute Units The standard unit of measure for the RESULT. Text National Institute of Standards and Technology Count Unit used to express the enumeration of the METRIC analyzed Producer defined The standard unit of measure for the RESULT. Text https://www.nist.gov/pml/weights-and-measures/metric-si/si-units StableIsotope_MethylMercury_TotalMercury_Analysis Text (txt) file containing information on methyl mercury and total mercury concentrations and light stable and total mercury stable isotope composition for split samples of seston, periphyton, benthic insects, emerging adult aquatic insects, riparian spiders, and fish. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples Producer Defined 2015 2016 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Sample_Type Literal value representing the common name of the type of organism included in the sample Producer Defined Terrestrial Vegetation Leaves of shrubs growing along the banks of streams Producer defined Aquatic Insect Larvae Whole bodies of juvenile stage aquatic insects Producer defined Biofilm Microbes scraped off rocks in the stream Producer defined Seston Small particles of detritus (organic and inorganic) suspended in the stream channel Producer defined Fish Muscle plug from lateral fillet of fish Producer defined Spider Whole bodies of riparian spiders living near streams Producer defined Sediment Grab sample of dominantly fine to coarse sand. Producer defined Aquatic Insect Adult Whole bodies of recently emerged adult aquatic insects Producer defined Taxa A literal value representing the lowest level of taxonomic hierarchy to which the sample organism was identified. In Linnaean classification system called the taxonomic hierarchy, which today has eight ranks of traits from general to specific: domain, kingdom, phylum, class, order, family, genus, and species. Text. Producer Defined Periphyton Freshwater organisms attached or clinging to plants and other objects projecting above the bottom sediments Producer defined ND No data Producer defined A literal value representing the lowest level of taxonomic hierarchy to which the sample organism was identified. In Linnaean classification system called the taxonomic hierarchy, which today has eight ranks of traits from general to specific: domain, kingdom, phylum, class, order, family, genus, and species. Text. Size_Class Literal value representing the range of dry weights of whole spiders grouped together Producer Defined ND No data Producer defined 0.5-4.0mg whole body dry weight of spiders included in sample ranged from 0.5 to 4.0 milligrams Producer defined GT11mg Whole body dry weight of spiders included in sample was greater than 11 milligrams Producer defined 4-11mg Whole body dry weight of spiders included in sample ranged from 4 to 11 milligrams Producer defined Fish_ID Numeric identifier of individual fish whose fillet was analyzed for chemistry Producer Defined ND No data. The The sample was not a fish. Producer defined Numeric identifier of individual fish whose fillet was analyzed for chemistry. Lab Alphabetic code indicating named laboratory in which chemical analysis of sample took place Producer Defined SIF USGS Stable Isotope Facility, Center for Mineral Resources Science, Denver, Colorado Producer defined MRL USGS Mercury Research Laboratory, Wisconsin Producer defined HRL USGS High-Resolution Laboratory; Geology, Geophysics, Geochemistry Science Center; Denver, Colorado Producer defined Metric A literal value representing the element measured. Text Producer Defined %C Percent of carbon in sample by dry mass Producer defined %MeHg Percent of total mercury in sample that is in the form of methyl mercury Producer defined %N Percent of nitrogen in sample by dry mass Producer defined C:N Ratio of carbon to nitrogen in sample by dry mass Producer defined d13C_VPDB Carbon isotopic composition of sample compared to PeeDee Beleminte, in ‰ Producer defined d15N_Air Nitrogen isotopic composition of sample compared to air, in ‰ Producer defined dC_adj Site-adjusted carbon isotopic composition of sample compared to biofilm-periphyton, in ‰ Producer defined dN_adj Site-adjusted nitrogen isotopic composition of sample compared to biofilm-periphyton, in ‰ Producer defined MeHg_ng_g Concentration of methyl mercury in sample, in nanograms per gram dry weight Producer defined THg_ng_g Concentration of total mercury in sample, in nanograms per gram dry weight Producer defined D199Hg Mass independent fractionation for Hg-199/198 of sample, in per mil (‰) Producer defined D200Hg Mass independent fractionation for Hg-200/198 of sample, in per mil (‰) Producer defined D201Hg Mass independent fractionation for Hg-201/198 of sample, in per mil (‰) Producer defined d199Hg Mercury-199/198 stable isotope composition of sample, in per mil (‰) Producer defined d200Hg Mercury-200/198 stable isotope composition of sample, in per mil (‰) Producer defined d201Hg Mercury-201/198 stable isotope composition of sample, in per mil (‰) Producer defined d202Hg Mercury-202/198 stable isotope composition of sample, in per mil (‰) Producer defined Result The analytical result for the METRIC measured. Numeric Producer Defined -35.40 201000 See UNITS attribute Units The standard unit of measure for the RESULT. Text National Institute of Standards and Technology (NIST) unitless The RESULT has no standard unit of measure Producer defined The standard unit of measure for the RESULT. Text. https://www.nist.gov/pml/weights-and-measures/metric-si/si-units EmergenceTrapInformation Text (txt) file containing information on characteristics of emergence trap sampling including time and date of deployment and depth of water under trap. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples Producer Defined 2015 2015 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Set_Date A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which sampling survey began. Numeric Producer Defined 20150723 20150724 Date Set_Time Time of day when sampling survey began, expressed in 24 hour clock. Mountain daylight savings time. Numeric Producer Defined 11:30 17:30 HH:MM Collect_Date A representation of time in which the smallest unit of measure is a day. The value is expressed in YYYYMMDD. It represents the date upon which sampling survey ended and samples were collected. Numeric Producer Defined 20150727 20150728 Date Collect_Time Time of day when sampling survey ended and samples were collected. Expressed in 24 hour clock. Mountain daylight savings time. Producer Defined 11:00 15:00 HH:MM Trap_No Numeric identifier for the trap within a stream reach Producer Defined Numeric identifier for the trap within a stream reach. This is a key attribute that ties to other data tables. Reach_Location A numeric identifier for the 10 meter segment within a 50 meter reach where samples where collected. "0" indicates upstream most limit of reach and "50" indicates downstream most limit of reach, in meters. Producer Defined 2 Segment sampled was located at 10 to 20 meters Producer defined 1 Segment sampled was located at 0 to 10 meters Producer defined 3 Segment sampled was located at 20 to 30 meters Producer defined 4 Segment sampled was located at 30 to 40 meters Producer defined 5 Segment sampled was located at 40 to 50 meters Producer defined SP 1-10 Segment located in adjacent reach, directly downstream of emergence reach sampled for spiders and benthic insects, at 0 to 10 meters in the spider reach. Producer defined Metric A literal value representing the element measured. Text Producer Defined Hole Indicates whether a hole was observed in the mesh netting of the emergence trap during deployment. Producer defined Pool_Area Estimated area of laminar flow on which the emergence trap was placed, reported in square meters. Producer defined Trap_Location Position of trap within 50 meter reach sampled for emergence, or in one case for spiders and benthic insects, reported in meters. Producer defined Water_Depth Depth of water under emergence trap, reported in meters. Producer defined Result The analytical result for the METRIC measured. Numeric Producer Defined Y Hole was observed in mesh netting of trap Producer defined N Hole was not observed in mesh netting of trap Producer defined 0 48 See UNITS attribute Units The standard unit of measure for the RESULT. Text National Institute of Standards and Technology (NIST) NA Not applicable Producer defined The standard unit of measure for the RESULT. Text. https://www.nist.gov/pml/weights-and-measures/metric-si/si-units EmergenceTrapCount Text (txt) file containing information for adult aquatic insects emerging into traps floating on stream surface including count and dry mass of emergence into traps grouped by taxonomic order, and family. Producer Defined Year A representation of time in which the smallest unit of measure is a year. The value is expressed in YYYY. It represents the year of the study in which samples Producer Defined 2015 2015 Year Field_Id An alpha/numeric identifier for the stream reach from which the sample was collected. Producer Defined An alpha/numeric identifier for the stream reach from which the sample was collected. This is a key attribute that ties to other data tables. Trap_No Numeric identifier for the trap within a stream reach Producer Defined Numeric identifier for the trap within a stream reach. This is a key attribute that ties to other data tables. Taxa A literal value representing the lowest level of taxonomic hierarchy to which the sample organism was identified. In Linnaean classification system called the taxonomic hierarchy, which today has eight ranks of traits from general to specific: domain, kingdom, phylum, class, order, family, genus, and species. Text. Producer Defined The lowest level of taxonomic hierarchy to which the sample organism was identified. In Linnaean classification system called the taxonomic hierarchy, which today has eight ranks of traits from general to specific: domain, kingdom, phylum, class, order, family, genus, and species. Count An enumeration of the benthic aquatic insects in the sample. Integer Producer Defined 1 621 observed individuals ND No data Producer defined Dry_Mass Mass of adult aquatic insects in sample, in milligrams dry weight. Numeric Producer Defined 0.039 50.004 milligrams dry weight Deployed Count of days emergence traps were deployed on the surface of the stream. Numeric Producer Defined 3.73 4.08 Days Trap_Area Surface area of the trap bottom in contact with stream surface, reported in square meters. Numeric Producer Defined 0.36 0.36 square meters CountFlux Count of aquatic aquatic insects in sample scaled to square meters of surface area of stream per day. Producer Defined ND No data Producer defined 0.7 422.8 observed individual per square meter DryMassFlux Mass of adult aquatic insects in sample, in milligrams dry weight per square meter of surface area of stream per day. Producer Defined 0.03 37.24 milligrams per square meter GS ScienceBase U.S. Geological Survey mailing and physical

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