Shannon White David Kazyak Robin Johnson Barbara Lubinski Michael Eackles 20230616 spreadsheet Reston, VA U.S. Geological Survey https://doi.org/10.5066/P943B0GT Shannon L. White Robin L. Johnson Barbara A. Lubinski Michael S. Eackles David C. Kazyak 2023 Report https://doi.org/10.3133/ofr20231054 Genotypes for Atlantic Sturgeon that were submitted by the National Marine Fisheries Service from individuals encountered as mortalities or taken during permitted activities. Genotypes were produced in order to perform individual-based assignment tests to determine natal origin. 2021 2025 ground condition Complete None planned World -82.6172 -57.4805 49.8380 24.3671 ISO 19115 Topic Category biota None Atlantic Sturgeon Microsatellite genotypes USGS Metadata Identifier USGS:61d834dad34ed79294009ea5 None Atlantic Coast None Atlantic sturgeon Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Actinopterygii Class Chondrostei Order Acipenseriformes Suborder Acipenseroidei Family Acipenseridae Subfamily Acipenserinae Genus Acipenser Species Acipenser oxyrinchus Subspecies Acipenser oxyrinchus oxyrinchus No access constraints. Please see 'Distribution Information' for details. These data are marked with a Creative Common CC0 1.0 Universal License. These data are in the public domain and do not have any use constraints. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. David C Kazyak U.S. Geological Survey, NORTHEAST REGION ECOLOGIST mailing address

11649 Leetown Road

Kearneysville WV 25430 US 304-724-4577 dkazyak@usgs.gov Genotyping error rates are expected to be very low. A positive control sample was included on each plate to check for success of PCR amplification and correct binning in fragment analysis software. A negative control sample was included on each plate to check for contamination of PCR products .All microsatellite scoring was automated and then checked by experienced laboratory personnel. PCR was performed again on all samples with missing data due to weak or unamplified alleles. PCR amplifications that had to be repeated were done with single loci and not in a multiplexed PCR. All Genemapper files were double-checked for scoring errors. All data are within expected range and were screened for duplication. Data are complete. DNA was extracted from preserved Atlantic sturgeon fin clips at the U.S. Geological Survey Eastern Ecological Science Center (USGS-EESC) in Kearneysville, WV. Whole genomic DNA was extracted from tissue samples using the Qiagen Blood and Tissue extraction kit (Qiagen, Valencia, CA, USA). All samples were screened for 12 Atlantic Sturgeon microsatellite loci (LS19, LS39, LS54, LS68, Aox12, Aox23, Aox45, AoxD170, AoxD188, AoxD165, AoxD44, AoxD241) 20231226 Genotypes_for Assignment_10September2025.csv Comma Separated Value (CSV) file containing data. Producer Defined Assigned Sample ID Unique ID for each individual Producer Defined Unique ID for each individual Collection Location General location where an individual was collected Producer Defined NA No collection data provided Producer defined Atlantic Ocean Individual collected at this location Producer defined James River, VA River, VA Individual collected at this location Producer defined Pamunkey River, VA Individual collected at this location Producer defined James River, VA Individual collected at this location Producer defined York River, VA Individual collected at this location Producer defined Other River or Lake - see comments section Individual collected at this location Producer defined NA No collection information provided Producer defined Long Island Sound, NY Individual collected at this location Producer defined Atlantic ocean Individual collected at this location Producer defined Rappahannock River, VA Individual collected at this location Producer defined Mattaponi River Individual collected at this location Producer defined Delaware Bay Individual collected at this location Producer defined Hudson River, NY Individual collected at this location Producer defined Cape Fear River, NC Individual collected at this location Producer defined Nanticoke River, Individual collected at this location Producer defined Delaware River, DE/PA Individual collected at this location Producer defined Latitude Latitude of collection location Producer Defined NA No collection latitude provided Producer defined Latitude of collection location Longitude Longitude of collection location Producer Defined NA No collection longitude provided Producer defined Longitude of collection location LS68 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 167 LS68 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 171 LS19 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 164 LS19 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 164 LS39 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 131 LS39 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 131 LS54 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 189 LS54 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 189 AO23 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 133 AO23 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 133 AO45 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 148 AO45 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 169 AO12 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 205 AO12 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 209 D170 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 160 D170 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 168 D188 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined << empty cell >> No Data Producer defined 0 353 D188 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined << empty cell >> No Data Producer defined 0 353 D165 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 226 D165 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 230 D44 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 186 D44 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 196 D241 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 270 D241 Microsatellite genotype (two scores per locus; "0" denotes a loci that was not scored) Producer Defined 0 298 U.S. Geological Survey - ScienceBase mailing address

Denver Federal Center

Building 810

Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P943B0GT None 20250929 David C Kazyak U.S. Geological Survey, NORTHEAST REGION ECOLOGIST mailing address

11649 Leetown Road

Kearneysville WV 25430 US 304-724-4577 dkazyak@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999