Simanonok, Michael P 20220415 Tabular digital data Jamestown, ND U.S. Geological Survey https://doi.org/10.5066/P9QA5UXU We measured nesting rates and nesting success for a community of cavity-nesting bees across gradients of floral abundance, floral richness, and land-cover in the Prairie Pothole Region. Variables in this dataset include a unique transect identifier, a count of the occupied nests per transect, total floral species richness per transect, total floral stem abundance per transect, bee species richness, number of emerged adult bees per transect, and the proportion of those emerged bees which were female. In addition, there are land-cover measurements of the total area, in hectares, of grasslands, wetlands, and woodlands within 500m and 1500m of each transect. We binned land-covers from the 2019 National Agricultural Statistics Service Cropland Data Layer into our definitions of grasslands (Other Hay/Non Alfalfa, Sod/Grass Seed, Fallow/Idle Cropland, and Grassland/Pasture), wetlands (Woody Wetlands and Herbaceous Wetlands), and woodlands (Deciduous Forest, Evergreen Forest, and Shrubland). We were interested in studying how nest occupancy and nesting success of cavity-nesting bees were related to nests located near managed grassland, wetlands, and woodlands, as well as how the nearby floral resources may influence nesting metrics. This dataset serves as a data release for a peer-reviewed publication, Simanonok, MP, Powley, M, Otto, CRV. 2022. Cavity-nesting bee nesting success across gradients of floral resources and land-cover. 20190523 20201001 ground condition Complete None planned -101.09069824176 -97.311401366907 48.871653692452 46.202343559879 USGS Thesaurus pollinators agriculture land use and land cover National Agricultural Library Thesaurus pollination land use grasslands Great Plains region bees pollinators ISO 19115 Topic Categories biota USGS Metadata Identifier USGS:613254c1d34e40dd9c0ac68a Getty Thesaurus of Geographic Names North Dakota Great Plains none none Michael P Simanonok U.S. Geological Survey Biologist mailing and physical

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Jamestown ND 58401 US 701-253-5514 msimanonok@usgs.gov We thank the collaborating landowners and USGS field technicians who supported this research. Funding for this research was provided in-part by the USDA-Farm Service Agency (17IAMRECRPHB1) and USDA-Natural Resources Conservation Service (673A7514178). Data were recorded on physical datasheets in the field, and were then checked by technicians and supervisors once entered into electronic spreadsheets. Data summaries were imported into R to identify and correct any errors. All values fall within expected and reasonable ranges. No duplicate entries were identified. There were no missing values. These data support the analyses performed in the associated manuscript. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. There are no positional data in these data. There are no positional data in these data. Using R as a Geographic Information System, we quantified the distribution of the land covers of interest (see attributes) within 500m and 1500m distances of each transect based on the 2019 Cropland Data Layer (CDL). We binned land-covers from the CDL which occurred in the 500m and 1500m distances together into categories of hectares grasslands (Other Hay/Non Alfalfa, Sod/Grass Seed, Fallow/Idle Cropland, and Grassland/Pasture), wetlands (Woody Wetlands and Herbaceous Wetlands), and woodlands (Deciduous Forest, Evergreen Forest, and Shrubland). 2021 Three times over the course of the summer in 2019, we identified flowering plants to species and quantified floral abundance, as number of floral stems, of each species for the transect. Because bee nest blocks were present at the transects throughout the summer (see below), our counts of floral stem abundance and richness are totaled per-transect for the entire season. 2021 Bee nesting blocks were constructed out of untreated 4x4” Douglas-fir, to final dimensions of 12” tall, with 21 holes drilled approximately 3” deep and 3/8” in diameter. Within each hole we placed parchment paper, cut to size, to allow for removal of nests at the end of the season. Nest blocks were secured to t-posts with zip-ties at a height of approximately 1m, with cavity entrances facing south-east. Nesting blocks were deployed to each of the 48 transects in early summer 2019 (5/23/19 to 7/15/19), and allowed to remain there until late in the season when they were collected (8/21/19 to 10/9/19). Despite date variability, nest blocks remained at transects for a minimum of 2 months. After the third floral count, nesting blocks were removed from the field. Occupied parchment paper nests were counted, nests were then removed from nest blocks and placed into individual, vented plastic vials where they were overwintered in an uninsulated storage building. Nests not easily removed from their blocks were overwintered in their blocks in order to preserve the nest and decrease the chance that damage from handling may artificially decrease emergence success. Beginning in April 2020, we periodically checked nests for emerging adult bees. When emergence was first detected, nests were moved indoors to room temperature conditions (ca. 22° C) where they were checked approximately daily for emergence. Nesting tubes continued to be observed for emergence approximately daily until October 2020. We considered bee metrics at the transect level, recording the number of tubes occupied out of 21 possible in each nest block, the number of bees which emerged, the proportion of emerged bees which were female, as well as bee species richness. Emerged bees were captured, euthanized via cyanide kill-jars, and identified to species utilizing a combination of dichotomous keys as well as comparison with a reference collection. 2020 NestingData Variables in this dataset include a unique transect identifier, a count of the occupied nests per transect, total floral species richness per transect, total floral stem abundance per transect, bee species richness, number of emerged adult bees per transect, and the proportion of those emerged bees which were female. In addition, there are land-cover measurements of the total area, in hectares, of grasslands, wetlands, and woodlands within 500m and 1500m of each transect. Originator defined transect Unique numeric identifier for each study transect. Transects were 2 x 20 meters in size. Producer defined. 1 48 count_occuped_nests The count of occupied nests within a nesting block at each transect. Producer defined. 0 21 floral_species_richness The number of blooming floral species detected at each transect. Producer defined. 1 22 floral_stem_abundance The total abundance of blooming floral stems counted at each transect. Producer defined. 1 8479 bee_species_richness The bee species richness of bees which successfully emerged from each nesting block at each transect after overwintering. Producer defined. 0 3 number_emerged_bees The total number of bees which emerged from a nesting block after overwintering. Producer defined. 0 39 proportion_female_bees The proportion of emerged bees which were female. Producer defined. 0 1 hectares_grassland500m The area of grassland land-covers, in hectares, within 500 meters of the transect. Producer defined. 2.79 66.15 hectares (ha) hectares_wetlands500m The area of wetland land-covers, in hectares, within 500 meters of the transect. Producer defined. 1.17 30.96 hectares (ha) hectares_woodlands500m The area of woodland land-covers within 500 meters of each transect. Producer defined. 0 3.87 hectares (ha) hectares_grassland1500m The area of grassland land-covers, in hectares, within 1500 meters of each transect. Producer defined. 17.46 574.83 hectares (ha) hectares_wetlands1500m The area of wetland land-covers, in hectares, within 1500 meters of a transect. Producer defined. 5.67 149.13 hectares (ha) hectares_woodlands1500m The area of woodland land-covers, in hectares, within 1500 meters of each transect. Producer defined. 0 18.18 hectares (ha) U.S. Geological Survey, ScienceBase U.S. Geological Survey mailing and physical

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Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. ASCII rfc4180 Comma separated values NestingDataRelease.csv 0.003 https://doi.org/10.5066/P9QA5UXU None 20220415 Michael P Simanonok U.S. Geological Survey Biologist mailing and physical

8711 37Th Street SE

Jamestown ND 58401 US 701-253-5514 msimanonok@usgs.gov Content Standard for Digital Geospatial Metadata FGDC-STD-001-1998