Daniel A. Grear 20210708 tabular digital data https://doi.org/10.5066/P990S43L Daniel A. Grear Brittany A. Mosher Katherine L.D. Richgels Evan H.C. Grant 202108 publication Biological Conservation vol. 260 n/a Elsevier BV ppg. 109222 https://doi.org/10.1016/j.biocon.2021.109222 Results of a literature search performed December 1, 2020. The tables in this document summarize the publications fitting in this search criteria and are split into the publications that update the list of international species that could carry Bsal (Appendix 1 Table S1). Number of individual live amphibians imported in categories of urodelan (salamander, newt, and related taxa) species restricted under the interim regulations and not listed, species not listed on the regulations that have had Bsal detected, notably Bombina spp., anuran species in the genus Rana, and Xenopus (including African clawed frogs) as a comparison of species not thought to be susceptible to Bsal, but commonly imported (Appendix 3 Table S1). Data and relative risk scores per county used for the risk analysis of B. salamandrivorans invasion, consequences, and surveillance for U.S. salamanders. All variables were scaled from 1, lowest relative risk, to 4, highest relative risk. Import and introduction risk scores were calculated from the average live imports per year for the pre-action risk period (2010-2015) and post-action risk period (2016-2019) (Appendix S3 Table S2). Our objectives were to evaluate the effects of regulatory action on the introduction of the amphibian fungal pathogen Batrachochytrium salamandrivorans (Bsal) to the U.S., quantify how a large-scale surveillance effort impacted consequence risk, and to combine other new information on species susceptibility to re-evaluate Bsal risk to the U.S. 20100101 20191031 observed Complete None planned -125.8594 -61.1719 50.2893 23.8858 United States ISO 19115 Topic Category biota None Bsal Bombina chytridiomycosis emerging infectious disease risk surveillance salamander wildlife trade USGS Metadata Identifier USGS:6030436dd34eb12031161ba2 Common geographic areas United States None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Daniel A Grear U.S. Geological Survey mailing address

6006 Schroeder Road

Madison WI 53711 US 608-270-2478 608-270-2415 dgrear@usgs.gov This study was funded by the U.S. Geological Survey Ecosystems Mission Area, Hazards Mission Area, and the U.S. Fish and Wildlife Service Bureau of Aquatic Invasive Species None Urodela Anura Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Amphibia Order Caudata Order Anura TSN: 173423 Manual checks for data that fall outside of expected range All data fall within expected ranges. All data included. Requested import data from USFWS. 20191001 AppendixS1TableS1.xlsx (SpeciesDetails) Excel Worksheet Producer Defined Family Family and sub-family taxonomy according to Amphibiaweb.org method: https://amphibiaweb.org/taxonomy/AWtaxonomy.html Producer Defined Family and sub-family taxonomy according to Amphibiaweb.org method: https://amphibiaweb.org/taxonomy/AWtaxonomy.html Subfamily Family and sub-family taxonomy according to Amphibiaweb.org method: https://amphibiaweb.org/taxonomy/AWtaxonomy.html Producer Defined Family and sub-family taxonomy according to Amphibiaweb.org method: https://amphibiaweb.org/taxonomy/AWtaxonomy.html Genus Genus according to what was reported in the paper Producer Defined Genus according to what was reported in the paper species species according to what was reported in the paper Producer Defined species according to what was reported in the paper Common name Common name, there is no standard naming convention for common names Producer Defined Common name, there is no standard naming convention for common names North American Yes = native north american species, no = not native north american speies Producer Defined Y Native to North America Producer defined N Not native to North America Producer defined PCR Bsal PCR detection, 1 = detected, 0 = not deteected, or not attempted Producer Defined 1 Bsal detected by PCR Producer defined 0 Bsal not detected by PCR Producer defined not attempted PCR not attempted Producer defined Histology 1 = histology consistent with Bsal infection, 0 = no evidence of Bsal infection on histology, or not attempted Producer Defined not attempted histology not attempted Producer defined 0 no histological evidence of Bsal infection Producer defined 1 histology consistent with Bsal infection Producer defined not tested not tested Producer defined Resistant Classification by criteria of Martel etal 2014 For the specific study and species, how the data would be classified according to the criteria stated in Martel et al. 2014: resistant=either was not infected or developed a short-term infection without clinical signs following exposure to Bsal; tolerant=maintains a more prolonged infection with no signs of disease; susceptible = becomes infected and has clinical signs of disease with the possibility of subsequent recovery; lethal = an animal that dies as a result of infection; or not applicable = data presented not sufficient to categorize resistance according to these criteria Producer Defined resistant either was not infected or developed a short-term infection without clinical signs following exposure to Bsal Producer defined susceptible becomes infected and has clinical signs of disease with the possibility of subsequent recovery Producer defined lethal an animal that dies as a result of infection Producer defined tolerant maintains a more prolonged infection with no signs of disease Producer defined not applicable data presented not sufficient to categorize resistance according to these criteria Producer defined USFWS Resistant Classification For the specific study and species: carrier = capable of transmitted Bsal, includes evidence of short-term infection, as well as Martel et al tolerant, susceptible, or lethal response; non-carriers = Martel et al. resistant category or no histologic or field surveillance to suggest short-term infection could occur; not applicable = data available not sufficient to evaluate carrier vs non-carrier criteria. Producer Defined carrier capable of transmitted Bsal, includes evidence of short-term infection, as well as Martel et al tolerant, susceptible, or lethal response Producer defined not applicable data available not sufficient to evaluate carrier vs non-carrier criteria Producer defined non-carrier Martel et al. resistant category or no histologic or field surveillance to suggest short-term infection could occur Producer defined Captive/Wild captive = data from captive animals; wild = from wild or free-ranging animals Producer Defined captive data from captive animals Producer defined captive and wild data from both captive and wild animals Producer defined wild data from wild animals Producer defined unknown unknown Producer defined Challenge yes = controled laboratory challenge with Bsal; no = not a controlled challenge Producer Defined yes controlled laboratory challenge with Bsal Producer defined no not a controlled laboratory challenge Producer defined Disease Outcome death = death attributed to Bsal infeciton; morbidity = sickness or lesions attributed to Bsal infection; morbidity/death = both morbidity and death reported; recovered = Bsal cleared with no evidence of infection or PCR detection following a confirmed infection course; no disease = no disease signs or evidence of infection via histopathology ; not observed = disease outcomes not observed or reported Producer Defined no disease no disease signs or evidence of infection via histopathology Producer defined morbidty sickness or lesions attributed to Bsal infection Producer defined morbidity sickness or lesions attributed to Bsal infection Producer defined death death attributed to Bsal infeciton Producer defined not observed disease outcomes not observed or reported Producer defined recovered Bsal cleared with no evidence of infection or PCR detection following a confirmed infection course Producer defined Case Definition For the specific study and species: Case definitions according to White et al 2016. Producer Defined Bsal present positive Bsal PCR or culture Producer defined Confirmed Bsal chytridiomycosis both histopathology and PCR or culture positive Producer defined Not detected no histological or molecular evidence of Bsal detected Producer defined Suspect Bsal chytridiomycosis histopathology consistent with Bsal chytridiomycosis in the absence of positive PCR or culture Producer defined not detected no histological or molecular evidence of Bsal detected Producer defined Reference First author, year Producer Defined First author, year Notes notes explaining categorization rationale or additional study details Producer Defined notes explaining categorization rationale or additional study details AppendixS3TableS1.xlsx (ImportTable) Excel Worksheet Producer Defined Port The international port of entry through which live animals were received in the format City, ST Producer Defined The international port of entry through which live animals were received in the format City, ST Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2010 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2010 Producer Defined 0 81559 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2011 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2011 Producer Defined 0 99898 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2012 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2012 Producer Defined 0 77676 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2013 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2013 Producer Defined 0 66453 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2014 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2014 Producer Defined 0 72326 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2015 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2015 Producer Defined 0 54949 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2016 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2016 Producer Defined 0 1040 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2017 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2017 Producer Defined 0 100 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2018 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2018 Producer Defined 0 137 live animal 1 Salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2019 Number of live animals imported into the continental U.S. of salamander species listed under statute 18 USC 42 implemented in 2016, during calendar year 2019, through October 31 Producer Defined 0 50 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2010 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2010 Producer Defined 0 8805 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2011 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2011 Producer Defined 0 8888 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2012 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2012 Producer Defined 0 5154 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2013 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2013 Producer Defined 0 4530 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2014 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2014 Producer Defined 0 1830 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2015 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2015 Producer Defined 0 950 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2016 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2016 Producer Defined 0 2 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2017 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2017 Producer Defined 0 36 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2018 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2018 Producer Defined 0 436 live animal 1 Salamander species not listed under statute 18 USC 42, during calendar year 2019 Number of live animals imported into the continental U.S. of salamander species not listed under statute 18 USC 42 implemented in 2016, during calendar year 2019, through October 31 Producer Defined 0 22 live animal 1 Bombina spp. during calendar year 2010 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2010 Producer Defined 0 227345 live animal 1 Bombina spp. during calendar year 2011 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2011 Producer Defined 0 200395 live animal 1 Bombina spp. during calendar year 2012 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2012 Producer Defined 0 230645 live animal 1 Bombina spp. during calendar year 2013 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2013 Producer Defined 0 142455 live animal 1 Bombina spp. during calendar year 2014 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2014 Producer Defined 0 129946 live animal 1 Bombina spp. during calendar year 2015 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2015 Producer Defined 0 90888 live animal 1 Bombina spp. during calendar year 2016 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2016 Producer Defined 0 67510 live animal 1 Bombina spp. during calendar year 2017 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2017 Producer Defined 0 54563 live animal 1 Bombina spp. during calendar year 2018 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2018 Producer Defined 0 26114 live animal 1 Bombina spp. during calendar year 2019 Number of live animals imported into the continental U.S. in the genus Bombina, during calendar year 2019, through October 31 Producer Defined 0 6073 live animal 1 Rana spp. during calendar year 2010 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2010 Producer Defined 0 1163739 live animal 1 Rana spp. during calendar year 2011 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2011 Producer Defined 0 591140 live animal 1 Rana spp. during calendar year 2012 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2012 Producer Defined 0 704014 live animal 1 Rana spp. during calendar year 2013 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2013 Producer Defined 0 1382678 live animal 1 Rana spp. during calendar year 2014 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2014 Producer Defined 0 1469043 live animal 1 Rana spp. during calendar year 2015 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2015 Producer Defined 0 1108003 live animal 1 Rana spp. during calendar year 2016 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2016 Producer Defined 0 1815116 live animal 1 Rana spp. during calendar year 2017 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2017 Producer Defined 0 1727661 live animal 1 Rana spp. during calendar year 2018 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2018 Producer Defined 0 1720802 live animal 1 Rana spp. during calendar year 2019 Number of live animals imported into the continental U.S. in the genus Rana, during calendar year 2019, through October 31 Producer Defined 0 2536329 live animal 1 Xenopus spp. during calendar year 2010 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2010 Producer Defined 0 25200 live animal 1 Xenopus spp. during calendar year 2011 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2011 Producer Defined 0 28050 live animal 1 Xenopus spp. during calendar year 2012 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2012 Producer Defined 0 35245 live animal 1 Xenopus spp. during calendar year 2013 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2013 Producer Defined 0 46945 live animal 1 Xenopus spp. during calendar year 2014 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2014 Producer Defined 0 40750 live animal 1 Xenopus spp. during calendar year 2015 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2015 Producer Defined 0 36460 live animal 1 Xenopus spp. during calendar year 2016 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2016 Producer Defined 0 35450 live animal 1 Xenopus spp. during calendar year 2017 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2017 Producer Defined 0 22360 live animal 1 Xenopus spp. during calendar year 2018 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2018 Producer Defined 0 21205 live animal 1 Xenopus spp. during calendar year 2019 Number of live animals imported into the continental U.S. in the genus Xenopus, during calendar year 2019, through October 31 Producer Defined 0 17110 live animal 1 AppendixS3TableS2.xlsx (Table_riskscores) Excel Worksheet Producer Defined County Name County or Parish unit name Producer Defined County or Parish unit name State State name Producer Defined State name FIPS Federal Information Processing Standard 5 digit county identification code Producer Defined FIPS Code (5-Digit State & County ID) FIPS Code Release Relative Risk (captive distribution, same as Richgels et al 2016 Pet Trade score) Relative risk score for Bsal release (Pet Trade) from Richgels et al. 2016, Producer Defined 1.0 4.0 Salamander imports within 26km buffer (2010-2015) Average of number of live salamanders per year imported through ports of entry within 26km of county centroid, calendar years 2010-2015 Producer Defined 0.0 79298.1666666667 live animals per year Salamander imports within 130km buffer (2010-2015) Average of number of live salamanders per year imported through ports of entry within 130km of county centroid, calendar years 2010-2015 Producer Defined 0.0 79298.1666666667 live animals per year Salamander imports within 260km buffer (2010-2015) Average of number of live salamanders per year imported through ports of entry within 260km of county centroid, calendar years 2010-2015 Producer Defined 0.0 79298.1666666667 live animals Salamander import score within 26km buffer (2010-2015) Relative risk score scaled from the average number of imports per year 2010-2015 imported through ports of entry within 26km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Salamander import score within 130km buffer (2010-2015) Relative risk score scaled from the average number of imports per year 2010-2015 imported through ports of entry within 130km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Salamander import score within 260km buffer (2010-2015) Relative risk score scaled from the average number of imports per year 2010-2015 imported through ports of entry within 260km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Salamander imports within 26km buffer (2016-2019) Average of number of live salamanders per year imported through ports of entry within 26km of county centroid, calendar years 2016-October 31, 2019. Average calculated per 3.83 years Producer Defined 0.0 272.584856396867 Salamander imports within 130km buffer (2016-2019) Average of number of live salamanders per year imported through ports of entry within 130km of county centroid, calendar years 2016-October 31, 2019. Average calculated per 3.83 years Producer Defined 0.0 273.107049608355 Salamander imports within 260km buffer (2016-2019c) Average of number of live salamanders per year imported through ports of entry within 260km of county centroid, calendar years 2016-October 31, 2019. Average calculated per 3.83 years Producer Defined 0.0 273.107049608355 Salamander import score within 26km buffer (2016-2019) Relative risk score scaled from the average number of imports per year 2016-October 31, 2019 imported through ports of entry within 26km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk scores were calculated with Ymin and Ymax from 2010-2015 to allow direct comparison of risk scores among time periods Producer Defined 1.0 2.49231992062816 Salamander import score within 130km buffer (2016-2019) Relative risk score scaled from the average number of imports per year 2016-October 31, 2019 imported through ports of entry within 130km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk scores were calculated with Ymin and Ymax from 2010-2015 to allow direct comparison of risk scores among time periods Producer Defined 1.0 2.49282702731815 Salamander import score within 260km buffer (2016-2019) Relative risk score scaled from the average number of imports per year 2016-October 31, 2019 imported through ports of entry within 260km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk scores were calculated with Ymin and Ymax from 2010-2015 to allow direct comparison of risk scores among time periods Producer Defined 1.0 2.49282702731815 Salamander import risk score (2010-2015) Average of the three buffer category risk scores, 26 km, 130 km, and 260 km, then rescaled between 1 and 4, 2010-2015. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Salamander import risk score (2016-2019) Average of the three buffer category risk scores, 26 km, 130 km, and 260 km, then rescaled between 1 and 4, 2016-2019. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 2.49248895619149 Bombina spp. imports within 26km buffer (2010-2015) Average of number of live imports per year of the genus Bombina imported through ports of entry within 26km of county centroid, calendar years 2010-2015 Producer Defined 0.0 170279.0 Bombina spp. imports within 130km buffer (2010-2015) Average of number of live imports per year of the genus Bombina imported through ports of entry within 130km of county centroid, calendar years 2010-2015 Producer Defined 0.0 170279.0 Bombina spp. imports within 260km buffer (2010-2015) Average of number of live imports per year of the genus Bombina imported through ports of entry within 260km of county centroid, calendar years 2010-2015 Producer Defined 0.0 170279.0 Bombina spp. imports within 26km buffer (2016-2019) Average of number of live individuals of the genus Bombina per year imported through ports of entry within 26km of county centroid, calendar years 2016-October 31, 2019. Average calculated per 3.83 years Producer Defined 0.0 39773.3681462141 Bombina spp. imports within 130km buffer (2016-2019) Average of number of live individuals of the genus Bombina per year imported through ports of entry within 130km of county centroid, calendar years 2016-October 31, 2019. Average calculated per 3.83 years Producer Defined 0.0 39773.3681462141 Bombina spp. imports within 260km buffer (2016-2019) Average of number of live individuals of the genus Bombina per year imported through ports of entry within 260km of county centroid, calendar years 2016-October 31, 2019. Average calculated per 3.83 years Producer Defined 0.0 39773.3681462141 Salamander + Bombina spp. import score within 26km buffer (2010-2015) Relative risk score scaled from the average number of salamander and Bombina spp. imports per year 2010-2015 imported through ports of entry within 26km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.30490548997733 Salamander + Bombina spp. import score within 130km buffer (2010-2015) Relative risk score scaled from the average number of salamander and Bombina spp. imports per year 2010-2015 imported through ports of entry within 130km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.30490548997733 Salamander + Bombina spp. import score within 260km buffer (2010-2015) Relative risk score scaled from the average number of salamander and Bombina spp. imports per year 2010-2015 imported through ports of entry within 260km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.30490548997733 Salamander + Bombina spp. import score within 26km buffer (2016-2019) Relative risk score scaled from the average number of salamander and Bombina spp. imports per year 2016-October 31, 2019 imported through ports of entry within 26km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk scores were calculated with Ymin and Ymax from 2010-2015 to allow direct comparison of risk scores among time periods Producer Defined 1.0 3.55830645049822 Salamander + Bombina spp. import score within 130km buffer (2016-2019) Relative risk score scaled from the average number of salamander and Bombina spp. imports per year 2016-October 31, 2019 imported through ports of entry within 130km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk scores were calculated with Ymin and Ymax from 2010-2015 to allow direct comparison of risk scores among time periods Producer Defined 1.0 3.55830959821346 Salamander + Bombina spp. import score within 260km buffer (2016-2019) Relative risk score scaled from the average number of salamander and Bombina spp. imports per year 2016-October 31, 2019 imported through ports of entry within 260km of county centroid, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk scores were calculated with Ymin and Ymax from 2010-2015 to allow direct comparison of risk scores among time periods Producer Defined 1.0 3.55830959821346 Salamander + Bombina spp. import risk score (2010-2015) Average of the three buffer category risk scores, 26 km, 130 km, and 260 km of salamander and Bombina spp. import, then rescaled between 1 and 4, 2010-2015. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.30490548997733 Salamander + Bombina spp. import risk score (2016-2019) Average of the three buffer category risk scores, 26 km, 130 km, and 260 km of salamander and Bombina spp. import, then rescaled between 1 and 4, 2016-2019. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 3.55830749973663 Introduction risk score salamander imports (2010-2015) Average of the import risk score and release relative risk score of salamander species, then rescaled between 1 and 4, 2010-2015. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Introduction risk score salamander imports (2016-2019) Average of the import risk score and release relative risk score of salamander species, then rescaled between 1 and 4, 2016-2019. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Introduction risk score salamander + Bombina spp. imports (2010-2015) Average of the import risk score and release relative risk score of salamander species, then rescaled between 1 and 4, 2010-2015. where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Risk score was calculated with Ymin and Ymax from 2010-2015 salamander only import score to allow direct comparison of risk scores Producer Defined 1.0 4.15963638218708 Introduction risk scoreb salamander + Bombina spp. imports (2016-2019) Risk score was calculated with Ymin and Ymax from 2016-2019 salamander only import score to allow direct comparison of risk scores Producer Defined 1.0 3.76874738206106 Mean Annual Temp (°C) Mean annual 30-year norm temperature Producer Defined NA no temperature data available Producer defined 0.4 24.47 degree Celsius Mean Annual Temp Score Risk score calculated as (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined NA No score calculated for counties with no temperature data available Producer defined 1.0 4.0 Mean July Temp (°C) Mean July annual 30-year norm temperature Producer Defined NA No temperature data available Producer defined 11.21 33.41 Mean July Temp Score with 25C max Risk score calculated as (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the absolute difference from 15 degrees and Ymax occurs at 15 and Ymin occurs with a value of 25 degrees or greater for each county with maximum , Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined NA No score calculated for counties with no temperature data available Producer defined 1.0 4.0 Mean July Temp Scoreb with 27C max Risk score calculated as (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the absolute difference from 15 degrees and Ymax occurs at 15 and Ymin occurs with a value of 27 degrees or greater for each county with maximum , Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined NA No score calculated for counties with no temperature data available Producer defined 1.0 4.0 Environmental Suitability scoreb with 25C max Average of mean annual temp and mean July temp score with a 25 degree threshold as min; rescaled between 1 and 4 Producer Defined NA No score calculated for counties with temperature data not available Producer defined 1.0 4.0 Environmental Suitability score with 27C max Average of mean annual temp and mean July temp score with a 27 degree threshold as min; rescaled between 1 and 4 Producer Defined NA No score calculated for counties with no temperature data available Producer defined 1.0 4.0 Species Richness Score Number of salamander species per county, per Richgels et al 2016, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum Producer Defined NA No score calculated for counties with wild salamander species Producer defined Number of salamander species per county, per Richgels et al 2016, where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum Consequences risk score with 25C max environmental suitability Average of introduction and consequences score with 25 degree environmental suitability threshold; rescaled between 1 and 4; where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum Producer Defined NA No score calculated for counties with wild salamander species Producer defined 1.0 4.01 Consequences risk score with 27C max environmental suitability Average of introduction and consequences score with 27 degree environmental suitability threshold; rescaled between 1 and 4; where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum Producer Defined NA No score calculated for counties with wild salamander species Producer defined 1.0 4.0 No. of surveillance samples collected per county Number of surveillance samples reported per county Producer Defined NA no samples reported for the county Producer defined 1.0 473.0 No. of sites sampled for surveillance per county Number of discrete sites reported per county where surveillance samples were collected Producer Defined NA No samples reported for the county Producer defined 1.0 21.0 Proportional decrease in occupancy (ψ) from surveillance, relative to non-informative ψ prior, δ Updated occupancy estimated from surveillance sample and sites per county compared to an occupancy estimate generated from no data and prior expectation of uniform probability of Bsal occupancy Producer Defined 0.0 0.336425978217023 Consequence risk score Average of species richness and environmental suitability with 25C max scores; rescaled between 1 and 4; where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum Producer Defined 1.0 4.01 Consequence risk score, conditional on surveillance (consq risk score *(1-δ)) Consequence risk score multiplied by the proportional decrease in occupancy, (1-delta) Producer Defined 0.986672045179508 4.01 Total Risk Score from salamander imports (2010-2015) Average of introduction with only salamander import and consequences score; rescaled between 1 and 4; where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Producer Defined 1.0 4.0 Total Risk Score from salamander and Bombina spp. imports (2010-2015) Average of introduction with salamander + Bombina spp. import and consequences score; rescaled between 1 and 4; where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Ymin and Ymax from introduciton score with salamander import only to allow direct comparison of scores Producer Defined 1.0 3.98041917592596 Total Risk Score from salamander and Bombina spp. Imports with surveillance (2016-2019) Average of introduction with salamander and Bombina spp. import and consequences score; rescaled between 1 and 4; where score = (Y - Ymin) x 3/(Ymax -Ymin) + 1 , where Y is the value of a factor for each county, Y min and Ymax are the minimum and maximum values for that factor, 3 is the new range (1-4) and 1 is the new minimum. Ymin and Ymax from introduciton score with salamander + Bombina spp. import only to allow direct comparison of scores Producer Defined 1.0 3.98896515641289 U.S. Geological Survey GS ScienceBase mailing address

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