Nathan A. Johnson Sean M. Keogh 20201209 tabular digital data https://doi.org/10.5066/P9LI4LKX Sean M. Keogh Nathan A. Johnson James D. Williams Charles R. Randklev Andrew M. Simons 20210312 publication n/a Wiley https://doi.org/10.1111/jbi.14066 Here we provide public access to three DNA sequence alignments (COI, ND1, ITS1) and details on all specimens utilized in Keogh et al. (in review). These data were collected to investigate phylogeographic patterns, species boundaries, and the demographic history of freshwater mussels in the Lampsilis teres species complex. Author ORCIDs: Johnson, N.A.(0000-0001-5167-1988) 20201001 ground condition Complete None planned -178.2100 179.7700 71.3500 18.9200 eastern United States ISO 19115 Topic Category biota none freshwater mussel conservation genetics DNA USGS Metadata Identifier USGS:5faaaf84d34eb413d5df1f36 None. Please see 'Distribution Info' for details. None. Users are advised to read the dataset's metadata thoroughly to understand appropriate use and data limitations. Nathan A Johnson U.S. Geological Survey, Southeast Region Research Biologist mailing address

7920 NW 71St Street

Gainesville FL 32653 US 352-264-3574 352-378-4956 najohnson@usgs.gov Funding for collection of these data was provided by the U.S. Geological Survey, Wally Dayton Wildlife Fellowship, Moos Graduate Research Fellowship in Aquatic Biology (University of Minnesota), Joyce Davenport Fellowship, Dayton Research Grant & Dayton Fellowship (Bell Museum of Natural History), and Minnesota Agricultural Experiment Station. None None None None None We used polymerase chain reaction (PCR) to amplify gene fragments, which were carried out in a solution containing 1.5 uL template DNA, 2.75 uL water, 6.25 uL GoTaq Green Master Mix (Promega, Madison, WI), and 1.0 uL of each forward and reverse primer. Purified PCR products were sequenced using ABI BigDye Terminator v3.1. Forward and reverse electropherograms were edited and assembled in Geneious v6.1.6 and aligned using the MUSCLE algorithm. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. 20201001 Nathan A Johnson U.S. Geological Survey, Southeast Region Research Biologist mailing address

7920 NW 71St Street

Gainesville FL 32653 US 352-264-3574 352-378-4956 najohnson@usgs.gov Appendix S1.csv Comma Separated Value (CSV) file containing data. Producer Defined Alignment_ID Unique identifier for each specimen and corresponding DNA alignment files Producer Defined Unique identifier for each specimen and corresponding DNA alignment files Genus Genus name for the specimen Producer Defined Genus name for the specimen Species Species name for the specimen Producer Defined Species name for the specimen Locality Detailed locality information describing location where specimen was collected Producer Defined Detailed locality information describing location where specimen was collected Drainage Major river basin of collection site Producer Defined NULL No Data Producer defined Major river basin of collection site Museum Museum institution where specimens were deposited or accessed Producer Defined NULL No Data Producer defined Museum institution where specimens were deposited or accessed Catalog_Number Museum catalog number Producer Defined NULL No Data Producer defined Museum catalog number COI_GenBank GenBank accession number for the COI locus Producer Defined GenBank accession number for the COI locus ND1_GenBank GenBank accession number for the ND1 locus Producer Defined NULL No Data Producer defined GenBank accession number for the ND1 locus ITS1_GenBank GenBank accession number for the ITS1 locus Producer Defined NULL No Data Producer defined GenBank accession number for the ITS1 locus Col_Day_DD The two-digit day the specimen was collected Producer Defined NULL No Data Producer defined 1 31 Days 1 Col_Month_MM The two-digit month the specimen was collected Producer Defined NULL No Data Producer defined 1 12 month 1 Col_Year_YYYY The four-digit year the specimen was collected Producer Defined NULL No Data Producer defined 2007 2018 year 1 Latitude Latitude of the collection site Producer Defined NULL No Data Producer defined Latitude of the collection site Longitude Longitude of the collection site Producer Defined NULL No Data Producer defined Longitude of the collection site DNA alignment files are provided in Phylip format. The filenames are as follows: • CO1.phy • ND1.phy • ITS1.phy The phylip file (.phy) is formatted as follows: the first line indicates the number of taxa and number of nucleotides and subsequent lines contain a taxon identifier (Genus.Species.Isolate) in the first column, and the DNA sequence in the second column. Also provide are the four phylogenetic tree files in newick format constructed from the DNA alignments listed above. The concatenated tree included data from all DNA loci. • COI_genetree • NDI_genetree • ITSI_genetree • Concatenated_tree none U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P9LI4LKX None 20201209 Nathan A Johnson U.S. Geological Survey, Southeast Region Research Biologist mailing address

7920 NW 71St Street

Gainesville FL 32653 US 352-264-3574 352-378-4956 najohnson@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999