James A Luoma Diane L Waller Todd J Severson Matt T Barbour Jeremy K Wise Eric G Lord Lynn Bartsch Michelle Bartsch Brian Gray 20200228 shapefile Online U.S. Geological Survey https://doi.org/10.5066/P9ZPKI64 James A Luoma Diane L Waller Todd J Severson Matt T Barbour Jeremy K Wise Lynn Bartsch Michelle Bartsch Brian Gray 20191222 publication Open File Report Open-File Report 2019-1126 https://doi.org/10.3133/ofr20191126 We applied Zequanox using a custom-engineered, boat mounted application system to replicated 0.30 Hectare plots within a small inland lake. The objectives of these applications were to determine if uncontained, open-water Zequanox applications could effectively control zebra mussel populations and protect native unionid mussel populations within zebra mussel infested systems. The datasets included are as follows: Exposure Water Chemistry Hardness and Alkalinity Native Mussel Sonde Water Chemistry Zebra Mussel Density Zebra Mussel Length Zebra Mussel Mortality Zequanox Concentration ShapeFiles: PLOTS, UNIONID, ZEQUANOX CONCENTRATION, ZM_DENSITY, ZM_MORTALITY Data were collected to monitor water quality during and after treatments, measure treatment impacts to native mussels and zebra mussels, quantitate Zequanox concentrations during treatment, spatially correlate concentration to mortality, density, native mussel impacts, and to investigate effects of the treatment on zebra mussel populations (density, length, and mortality). 20170707 20180717 ground condition Complete None planned -84.8987 -84.8807 45.4129 45.4068 Round Lake, Emmet County, Michigan, USA None Zebra mussel Zequanox USGS Metadata Identifier USGS:5e593e63e4b01d50924a9b58 None Round Lake, Emmet County, Michigan, USA none none James A Luoma U.S. Geological Survey Research Fish Biologist mailing address

2630 Fanta Reed Road

La Crosse WI 54603 United States 608-781-6391 608-783-6066 jluoma@usgs.gov Funding through GLRI and USGS appropriated funds. Patty Reis (UMESC), Seth Donovich and Carrie Link (Marrone Bio Innovations), Matt Claucherty, David Edwards (Tip of the Mitt Watershed Council), Darrin McCullough (MI DEQ), Barbara Bennie (University of Wisconsin-La Crosse) ITIS Dreissena polymorpha Lampsilis siliquoidea Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Lophozoa Phylum Mollusca Class Bivalvia Subclass Heterodonta Order Veneroida Superfamily Dreissenoidea Family Dreissenidae Genus Dreissena Species Dreissena polymorpha TSN: 81339 Subclass Palaeoheterodonta Order Unionoida Superfamily Unionoidea Family Unionidae Subfamily Ambleminae Tribe Lampsilini Genus Lampsilis Species Lampsilis siliquoidea TSN: 80028 Data proofing, instrument calibrations All data matches up with details provided. All values fall within expected ranges except one glycogen measurement was omitted for being over a concentration threshold . All data has been proofed a number of times for duplicates and omissions. See explanation tabs in excel summary files Multiple GPS readings per point as measured with RTK system with base station None was needed – no vertical data recorded 3 sampling points were indiscriminately selected within each zone of each plot. Each point was sampled at 1 hour, 2, 4, 6, and 8 hours after the midpoint of the application in both treated and untreated plots. Samples were collected with a peristaltic pump from a float located on the shore-side of the plots via 250’ of ¼ inch airline. The airline ran to a rigid screen tube with large pore filter bag material secured over it to prevent detritus and other materials from clogging the lines. Lines were purged before each sample was collected to ensure an accurate representation of the sampling period. Samples were measured on a spectrophotometer (wavelength = 660 nanometers against a standard curve to determine the concentration of Zequanox in milligrams per liter as active ingredient. The location of each sampling point was measured and recorded using a Trimble RTK-GPS system. Proofed data were attached to the respective spatial reference data and a shapefile was created. 20170707 20170707-20181101 process data 20170707 Vector Entity point 54 Universal Transverse Mercator 16 0.9996 -87.0 0.0 500000.0 0.0 coordinate pair 0.6096 0.6096 meters WGS_1984 WGS_84 6378137.0 298.257223563 ZEQUANOX_CONCENTRATION.shp Attribute Table Table containing attribute information associated with the data set. Producer defined FID Internal feature number. ESRI Sequential unique whole numbers that are automatically generated. Shape Feature geometry. ESRI Coordinates defining the features. PLOT there were a total of 6 individual plots within the study lake. Each plot is a square (~55 x 55 meters and ;~0.304 hectares in area) Producer defined 1 6 plot ZONE Edge, Fringe, or Core; divides each plot into 3 separate zones. Edge is the outer most 1 meter around plot; Fringe is from 1 to 4 meters around plot; Core is everything inside of Fringe Producer defined C Core is everything inside of Fringe Producer defined E Edge is the outer most 1 meter around plot Producer defined F Fringe is from 1 to 4 meters around plot Producer defined REPLICATE NUMBER each sample was collected 3 individual times Producer defined 1 3 replicate number TREATMENT Treated or Control; Treated received Zequanox treatment, Controls received no Zequanox Producer defined T Treated received Zequanox treatment Producer defined C Controls received no Zequanox Producer defined EASTING Easting Producer defined 664435.259 665826.99 Meter NORTHING Northing Producer defined 5030328.004 5030971.511 Meter CONC_1H Zequanox concentration 1 hour after application midway point (milligrams per liter as active ingredient) Producer defined 0.0 106.65 milligrams per liter as active ingredient CONC_2H Zequanox concentration 2 hour after application midway point (milligrams per liter as active ingredient) Producer defined 0.0 85.521 milligrams per liter as active ingredient CONC_4H Zequanox concentration 4 hour after application midway point (milligrams per liter as active ingredient) Producer defined 0.0 37.372 milligrams per liter as active ingredient CONC_8H Zequanox concentration 8 hour after application midway point (milligrams per liter as active ingredient) Producer defined 0.0 6.906 milligrams per liter as active ingredient U.S. Geological Survey - ScienceBase mailing and physical

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. 20210608 James A Luoma U.S. Geological Survey Research Fish Biologist mailing address

2630 Fanta Reed Road

La Crosse WI 54603 United States 608-781-6391 608-783-6066 jluoma@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999