Stewart, A.R. Dal Porto, D. Wagner, C. Godbout, J. Solomon, A. Kakouros, E. 20191220 Excel and CSV spreadsheets Menlo Park, CA U.S. Geological Survey https://doi.org/10.5066/P9GI73P9 Stewart, A. Robin Feyrer, Frederick Johnson, Rachel. C. 2019 Manuscript Online Elsevier B.V. https://doi.org/10.1016/j.scitotenv.2019.135919 Estuaries provide critical habitat for a vast array of fish and wildlife but are also a nexus for core economic activities that mobilize and concentrate contaminants that can threaten aquatic species. Selenium (Se), an essential element and potent reproductive toxin, is enriched in parts of the San Francisco Estuary (SFE) to levels known to cause toxicity, yet the risk of Se to species that inhabit the SFE is not well understood. We quantified Se concentrations in muscle, liver, and ovary of the demersal cyprinid Sacramento Splittail from six regions in the SFE at three time points (fall 2010-11, spring 2017) to evaluate Se exposure risk. Here we report fish morphological attributes, total selenium concentrations in fish boneless skinless muscle, liver, and ovary, as well as carbon, nitrogen and sulfur stable isotope values and elemental mass concentrations in fish muscle. Data were collected to understand selenium regional distributions and exposure risk to Sacramento Splittail to inform regulatory and management decisions. 20101115 20111208 20170105 20170510 ground condition Complete None planned -122.75573730338 -121.11877441281 38.470451156149 37.84848701694 Petaluma River, Napa River, Suisun Bay and Sacramento River California USGS Thesaurus selenium bioaccumulation biological monitoring cyprinid estuary delta exposure risk USGS Metadata Identifier USGS:5db7525de4b0b0c58b5a4954 Geographic Names Information System (GNIS) San Francisco Bay Sacramento-San Joaquin Delta none none Robin Stewart WATER: ESP HEIB Research Hydrologist mailing and physical

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Menlo Park CA 94025 US 650-329-4550 arstewar@usgs.gov EPA Rare Grant (DW1492426401), U.S. Fish and Wildlife Service (IAA4500109746), U.S. Geological Survey (USGS) Water Mission Area - Hydrological Ecological Interactions Branch, and in-kind support by NOAA Fisheries. Bureau of Reclamation funded the 2017 field collections (IA#R15PG00085) USGS Biocomplexity Thesaurus Fishes Integrated Taxonomic Information System (ITIS) 2019 ONLINE_REFERENCE Washington, D.C. Integrated Taxonomic Information System (ITIS) http://itis.gov Frederick V Feyrer SOUTHWEST REGION: PROJ OFFC-WATERSHED,ECOL,INTG MOD Fish Biologist mailing and physical

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Sacramento CA 95819 US 916-278-3232 ffeyrer@usgs.gov expert advice Sacramento Splittail are endemic to the San Francisco Estuary. A local expert on this species identified individual fish used in this study. The fish used in this study was identified to the level of genus and species. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Actinopterygii Class Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Pogonichthys Species Pogonichthys macrolepidotus splittail Sacramento splittail No formal attribute accuracy tests were conducted No formal logical accuracy tests were conducted Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted No formal positional accuracy tests were conducted Data were complied into Excel spreadsheets 20191210 both Fish were collected according to methods described in Feyrer F., Hobbs J., Acuna S., Mahardja B., Grimaldo L., Baerwald M., et al., 2015. Metapopulation structure of a semi-anadromous fish in a dynamic environment. Canadian Journal of Fisheries and Aquatic Sciences. 72, 709-721. https://doi.org/10.1139/cjfas-2014-0433. Samples were processed and analyzed according to the methods described in Kleckner A.E., Kakouros E., Stewart A.R., 2017. A practical method for the determination of total selenium in environmental samples using Isotope Dilution-Hydride Generation-Inductively Coupled Plasma-Mass Spectrometry. Limnology and Oceanography: Methods. 15, 363-371. https://doi.org/10.1002/lom3.10164. Geographic Names Information System (GNIS) place names Point 5.0 5.0 Decimal degrees T1_Splittail_data_dictionary This table defines all terms for all data tables (Tables 2-5) in this data release. A. Robin Stewart Table 1, columns 1-14 Table No., Table Name, Table Column, Column heading, Column description, Extended Description, Unit Abbreviation, Unit Definition, Additional Column Note, Range of Value (if applicable) (minimum and maximum), Analytical Laboratory Name, Analytical Laboratory Location, Method Reference-Primary, Method Reference-Secondary Producer defined All column headings are defined in the data dictionary for all data tables. T2_Splittail_Morp_Se_SIA Morphological, selenium and stable isotope data for individual Sacramento Splittail collected in the San Francisco Estuary. A. Robin Stewart Table 2, columns 1-52 All terms are defined in data dictionary (Table 1). Producer defined All column headings are defined in the data dictionary for all data tables. T3_Splittail_QA_QC_Blanks Procedural blank selenium concentrations for individual analysis dates. A. Robin Stewart Table 3, columns 1-5 All terms are defined in data dictionary (Table 1). Producer defined All column headings are defined in the data dictionary for all data tables. T4_Splittail_QA_QC_Precision Precision of selenium (Se) sample analyses. A. Robin Stewart Table 4, columns 1-7 All terms are defined in data dictionary (Table 1). Producer defined All column headings are defined in the data dictionary for all data tables. T5_Splittail_QA_QC_CRM Recovery of Certified Reference Materials (CRM) A. Robin Stewart Table 5, columns 1-8 All terms are defined in data dictionary (Table 1) Producer defined All column headings are defined in the data dictionary for all data tables ScienceBase U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 US 888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data for other purposes, nor on all computer systems, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. 20200827 Robin Stewart WATER: ESP HEIB Research Hydrologist mailing and physical

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Menlo Park CA 94025 US 650-329-4550 arstewar@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999