Kylle A. Roy Carter T. Atkinson Carolina Granthon 20190226 tabular digital data https://doi.org/10.5066/P9QN1HBT Carolina Granthon Kylle Roy Carter T. Atkinson 2019 publication Technical Report HCSU-087 University of Hawaii at Hilo Hawaii Cooperative Studies Unit http://hdl.handle.net/10790/4568 We designed two new samplers for monitoring airborne particulates, including fungal and fern spores and plant pollen, that rely on natural wind currents (Passive Environmental Sampler) or a battery operated fan (Active Environmental Sampler). Both samplers are modeled after commercial devices such as the Rotorod® and the Burkard® samplers, but are more economical and require less maintenance than commercial devices. We conducted wind tunnel comparisons of our two new samplers to Rotorod® samplers using synthetic polyethylene spheres (12 - 160 µm in diameter) to compare numbers and size range of particulates that are captured by the samplers. This dataset contains raw particle sizes of polyethylene spheres that were captured by the samplers during eight separate trials in a sealed room with constant recirculating air flow. These data were collected to compare sizes of polyethylene particles captured by Active, Passive and Rotorod® samplers. This comparison will help to determine their relative effectiveness for environmental sampling. 20180308 publication date Complete Annually -155.26051 -155.22738 19.42944 19.39342 Kilauea Field Station, Hawaii National Park, Hawaii ISO 19115 Topic Category environment None Environmental DNA Spore Traps Environmental Sampler USGS Metadata Identifier USGS:5c1c1d83e4b0708288c7ac29 None Hawaiii None. Please see 'Distribution Info' for details. None. Users are advised to read the data set's metadata thoroughly to understand appropriate use and data limitations. Kylle Roy USGS Geneticist mailing and physical

1 Crater Rim Drive

Hawaii National Park HI 96718 US 8089856437 kroy@usgs.gov Tom Harrington from Iowa State University provided Rotorod® Samplers and supplies. This work was funded in part by the U.S. Geological Survey Natural Resource Protection Program, the U.S. Department of Interior and U.S. Department of Agriculture joint Service First Program, and the U.S. Geological Survey Ecosystems Mission Area, Windows 10 Enterprise, Excel 2016 Multiple replicates were measured for each slide and rod. All data is logical. These data represent a controlled study of particles, not in a outside environment. NA NA Two fans with combined wind speeds of about 1m/second were placed in the back of a 5.5 x 2.3 x 3 m room. Two, 2.4 m tall, .05 m diameter, electrical conduit metal poles affixed in cinder blocks were placed in the middle of the room. Paired Passive, Active, and Rotorod® model 20 samplers were attached to the conduit adjacent to one another at ~140, ~180, and ~220 cm above the floor. The three pairs of samplers were rotated between the three vertical pole positions for different trials. One hundred mg of Cospheric microtechnology polyethylene beads ranging in size from 12-160 µm diameter were launched from a modified 10 ml serological pipette with compressed air and allowed to circulate in the room for each 4-hour trial. After each trial, slides and rods were collected, paired samplers were rotated between positions on the poles, and slides and rods were replaced. Slides and rods were examined and bead sizes were measured with CellSens software (version 1.9, Olympus Life Sciences, Tokyo, Japan) under a 10X objective and 10X ocular lens on an Olympus BH50 compound microscope. 20180801 PCount.csv Comma Separated Value (CSV) file containing data. Producer Defined TrialNumber Number assigned to each trial, in the order they occurred. Producer Defined 1 8 TrapPosition Trap location within the setup. Producer Defined Middle Trap positioned 215cm up the metal pole setup. Producer defined Top Trap positioned 230cm up the metal pole setup. Producer defined Bottom Trap positioned 200cm up the metal pole setup. Producer defined TrapType The design used for each trap. Producer Defined Active Trap with continuous air flow generated by a fan. Producer defined Passive Trap with a vane moving with wind direction. Producer defined Rotorod Commercial trap attached to a battery. Producer defined TrapOrder ID for each trap replicate. Producer Defined A First replicate for a particular trap. Producer defined B Second replicate for a particular trap. Producer defined Slide(or Rod)Number The order of each slide within a trap (out of 4), starting from the top. The order of each rod within a trap (out of 2), starting from the left. Producer Defined 1 4 ParticleCount Total number of polyethylene beads on each slide or rod. Producer Defined << empty cell >> No Data Producer defined 0 1366 Particles TrapComparisonSphereParticleSize.csv Comma Separated Value (CSV) file containing data. Producer Defined Number Individual identifier for each data entry. Producer Defined 1 4782 1 TrialNumber Number assigned to each trial, in the order they occurred and pre-release category Producer Defined 1 Trial 1 Producer defined 2 Trial 2 Producer defined 3 Trial 3 Producer defined 4 Trial 4 Producer defined 5 Trial 5 Producer defined 6 Trial 6 Producer defined 7 Trial 7 Producer defined 8 Trial 8 Producer defined Pre-release Measurements of polyethylene beads from the stock container and before beads were released. Producer defined SamplerType The design used for each sampler Producer Defined NA This column does not apply to the pre-release data and is therefore not applicable. Producer defined Active Battery operated sampler with continuous air flow generated by a fan. Producer defined Passive Sampler with a vane moving with wind direction. Producer defined Rotorod Battery operated commercial sampler. Producer defined SamplerOrder Identification number for each sampler replicate Producer Defined NA This column does not apply to the pre-release data and is therefore not applicable. Producer defined A First replicate for a particular sampler. Producer defined B Second replicate for a particular sampler. Producer defined Slide(or Rod)Number The order of each slide within a sampler (out of either 4 or 2), For Passive Samplers numbering starts from the top for a total of four slides. For Rotorod Samplers, numbering starts from the left for a total of 2 rods. Producer Defined NA This column does not apply to the pre-release data and is therefore not applicable. Producer defined 1 4 1 ParticleNumber Number assigned to particles measured within a particular slide or rod Producer Defined NA This column does not apply to the pre-release data and is therefore not applicable. Producer defined 1 143 particle 1 ParticleLength(µm) Length of individual polyethylene particles found on each slide or rod Producer Defined 1.42 169.98 micrometers 0.01 U.S. Geological Survey GS ScienceBase mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/P9QN1HBT None 20200827 Kylle A Roy U.S. Geological Survey, Pacific Island Ecosystem Research Center Geneticist mailing address

Bldg 344 Chain of Craters Rd.

Hawaii National Park HI 96718 US 808-985-6437 808-967-8568 kroy@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999