J. Hardin Waddle Daniel A. Grear Brad M. Glorioso Adam R. Backlin William J. Barichivich Adrianne B. Brand Daniel L. Calhoun Jillian E. Fleming Andrew E. Dietrich Patrick M. Kleeman Marcella A. Cruz 20190522 tabular digital data https://doi.org/10.5066/P9BGQA1T Daniel A. Grear Michael J. Adams Adam R. Backlin William J. Barichivich Adrianne B. Brand Gary M. Bucciarelli Daniel L. Calhoun Tara Chestnut Jon M. Davenport Andrew E. Dietrich Graziella DiRenzo Robert N. Fisher Brad M. Glorioso Evan H. Campbell Grant Brian J. Halstead Marc P. Hayes Blake R. Hossack Morgan Kain Patrick M. Kleeman Jeffrey M. Lorch Brome McCreary David A.W. Miller Brittany A. Mosher Erin Muths Christopher A. Pearl Charles W. Robinson Mark F. Roth Jennifer C. Rowe Walt Sadinski Brent H. Sigafus Iga Stasiak Samuel Sweet J. Hardin Waddle Susan C. Walls Gregory J. Watkins-Colwell Lori A. Williams Megan E. Winzeler 20241130 publication Ecological Monographs Wiley This dataset provides the results of a national survey of the United States for the salamander chytrid fungus Batrachochytrium salamandrivorans (Bsal) and the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) conducted by the U.S. Geological Survey Amphibian Research and Monitoring Initiative from May 2014 to August 2017. Sites were sampled by capturing amphibians by hand or by traps or nets that were then swabbed individually using methods that prevent sample contamination. All swabs were then analyzed using a real-time TaqMan PCR for detection of B. salamandrivorans and Bd on the extracted DNA. The data consist of locality information and data on the individual sampled, as well as the result of the test for B. salamandrivorans and Bd. No cases of B. salamandrivorans were detected in this study. These data were collected to attempt to document the current distribution of Batrachochytrium salamandrivorans (Bsal) in the United States Locality data can be used to determine the spatial extent of this survey. No cases of B. salamandrivorans were detected in this study. The number of individuals sampled per site can be used to determine the level of sampling effort at each site. The species data provides information on the species that were sampled. We also tested the samples for Batrachochytrium dendrobatidis (Bd), another amphibian fungal pathogen. Author ORCIDs: Waddle, J.H.(0000-0003-1940-2133);Grear, D.A.(0000-0002-5478-1549);Glorioso, B.M.(0000-0002-5400-7414);Backlin, A.R.(0000-0001-5618-8426);Barichivich, W.J.(0000-0003-1103-6861);Brand, A.B.(0000-0003-2664-0041);Calhoun, D.L.(0000-0003-2371-6936);Fleming, J.E.(0000-0003-2570-914X);Kleeman, P.M.(0000-0001-6567-3239);Cruz, M.A.(0000-0002-8100-8738) 20140502 20170817 ground condition Complete None planned United States of America -125.2441 -66.8848 49.3251 23.9662 ISO 19115 Topic Category biota USGS Thesaurus wildlife disease amphibians USGS Metadata Identifier USGS:5af5ef0de4b0da30c1b5f9eb None United States Common geographic areas United States None Ambystoma maculatum Ambystoma opacum Ambystoma cingulatum Ambystoma annulatum Ambystoma californiense Ambystoma gracile Ambystoma laterale Ambystoma macrodactylum Ambystoma mavortium Ambystoma talpoideum Ambystoma tigrinum Amphiuma means Amphiuma tridactylum Anaxyrus boreas Anaxyrus punctatus Aneides lugubris Aneides vagrans Batrachoseps attenuatus Batrachoseps gavilanensis Batrachoseps major Batrachoseps minor Batrachoseps nigriventris Batrachoseps pacificus Batrachoseps robustus Batrachoseps stebbinsi Dicamptodon ensatus Dicamptodon tenebrosus Ensatina eschscholtzii Eurycea bislineata Eurycea cirrigera Gyrinophilus porphyriticus Lithobates catesbeianus Lithobates chiricahuensis Lithobates pipiens Necturus beyeri Necturus maculosus Notophthalmus perstriatus Notophthalmus viridescens Plethodon cinereus Plethodon glutinosus Pseudacris cadaverina Pseudacris maculata Pseudacris regilla Pseudacris triseriata Pseudotriton ruber Rana aurora Rana luteiventris Rana muscosa Siren intermedia Spea hammondii Taricha granulosa Taricha sierrae Taricha Taricha torosa Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Amphibia Order Caudata Family Ambystomatidae Genus Ambystoma Species Ambystoma maculatum TSN: 173590 Species Ambystoma opacum TSN: 173591 Species Ambystoma cingulatum TSN: 173596 Species Ambystoma annulatum TSN: 173594 Species Ambystoma californiense TSN: 173595 Species Ambystoma gracile TSN: 173597 Species Ambystoma laterale TSN: 173599 Species Ambystoma macrodactylum TSN: 173601 Species Ambystoma mavortium TSN: 668193 Species Ambystoma talpoideum TSN: 173604 Species Ambystoma tigrinum TSN: 173592 Genus Dicamptodon Species Dicamptodon ensatus TSN: 173743 Species Dicamptodon tenebrosus TSN: 550242 Family Amphiumidae Genus Amphiuma Species Amphiuma means TSN: 173609 Species Amphiuma tridactylum TSN: 173612 Family Plethodontidae Subfamily Plethodontinae Genus Aneides Species Aneides lugubris TSN: 173703 Species Aneides vagrans TSN: 668242 Genus Ensatina Species Ensatina eschscholtzii TSN: 173732 Genus Plethodon Species Plethodon cinereus TSN: 173649 Species Plethodon glutinosus TSN: 173650 Subfamily Bolitoglossinae Genus Batrachoseps Species Batrachoseps attenuatus TSN: 173706 Species Batrachoseps gavilanensis TSN: 668243 Species Batrachoseps major TSN: 208343 Species Batrachoseps minor TSN: 668246 Species Batrachoseps nigriventris TSN: 173708 Species Batrachoseps pacificus TSN: 173709 Species Batrachoseps robustus TSN: 668247 Species Batrachoseps stebbinsi TSN: 173711 Subfamily Spelerpinae Genus Eurycea Species Eurycea bislineata TSN: 173685 Species Eurycea cirrigera TSN: 550246 Genus Gyrinophilus Species Gyrinophilus porphyriticus TSN: 173715 Genus Pseudotriton Species Pseudotriton ruber TSN: 173680 Family Proteidae Genus Necturus Species Necturus beyeri TSN: 173629 Species Necturus maculosus TSN: 173630 Family Salamandridae Genus Notophthalmus Species Notophthalmus perstriatus TSN: 173618 Species Notophthalmus viridescens TSN: 173615 Genus Taricha TSN: 173619 Species Taricha granulosa TSN: 173620 Species Taricha sierrae TSN: 775939 Species Taricha torosa TSN: 173622 Family Sirenidae Genus Siren Species Siren intermedia TSN: 173736 Order Anura Family Bufonidae Genus Anaxyrus Species Anaxyrus boreas TSN: 773513 Species Anaxyrus punctatus TSN: 773527 Family Ranidae Genus Lithobates Species Lithobates catesbeianus TSN: 775084 Species Lithobates chiricahuensis TSN: 775086 Species Lithobates pipiens TSN: 775108 Genus Rana Species Rana aurora TSN: 173446 Species Rana luteiventris TSN: 550546 Species Rana muscosa TSN: 173454 Family Hylidae Subfamily Hylinae Genus Pseudacris Species Pseudacris cadaverina TSN: 550237 Species Pseudacris maculata TSN: 207312 Species Pseudacris regilla TSN: 207313 Species Pseudacris triseriata TSN: 173525 Family Scaphiopodidae Genus Spea Species Spea hammondii TSN: 206990 None. Please see 'Distribution Info' for details. None. Users are advised to read the data set's metadata thoroughly to understand appropriate use and data limitations. Hardin Waddle U.S. Geological Survey, Southeast Region Research Ecologist mailing address

7920 NW 71St Street

Gainesville FL 32653 US 352-264-3524 352-378-4956 waddleh@usgs.gov M. Blooi F. Pasmans J. E. Longcore A. Spitzen-van der Sluijs F. Vercammen A. Martel 20131009 publication Journal of Clinical Microbiology vol. 51, issue 12 n/a American Society for Microbiology ppg. 4173-4177 http://dx.doi.org/10.1128/JCM.02313-13 AD Hyatt DG Boyle V Olsen DB Boyle L Berger D Obendorf A Dalton K Kriger M Hero H Hines R Phillott R Campbell G Marantelli F Gleason A Colling 20070118 publication Diseases of Aquatic Organisms vol. 73 n/a Inter-Research Science Center ppg. 175-192 http://dx.doi.org/10.3354/dao073175 Pascale Van Rooij An Martel Joachim Nerz Sebastian Voitel Filip Van Immerseel Freddy Haesebrouck Frank Pasmans 20110913 publication EcoHealth vol. 8, issue 2 n/a Springer Nature ppg. 237-243 http://dx.doi.org/10.1007/s10393-011-0704-z The attributes included are measured using standard techniques and methods. No methods were used to assess the accuracy of the location coordinates or the body length of sampled individuals. We collected two separate swabs from each animal, with one held in reserve in case Bsal was detected to provide confirmation. Bsal was not detected. All data in this release were checked for logical consistency. We ensured that all values were in ranges that were possible, and we checked carefully for duplicates. The dataset is complete. All records from the United States during this sampling period that we had access to were included. All sampling was conducted by capturing target animals by hand, nets, or traps. After capture, we handled each animal separately using disposable powderless-vinyl gloves and new, clean plastic bags to avoid cross contamination. We rubbed rayon-tipped sterile swabs (MW-113, Medical Wire & Equipment, Corsham, England) over the plantar side of one front limb, the plantar side of one hind limb, the ventral tail surface of caudates, the dorsal side of the body, and the ventral surface of the body 5 times each (Van Rooij et al 2011). We placed the swabs into sterile plastic vials with 20 μl of sterile deionized water. We recorded the snout-vent length and sex. We chilled swabs immediately after field collection and froze them at -20 C or colder. Swabs were sent frozen to the National Wildlife Health Center in Madison, Wisconsin for analysis. We extracted DNA from swabs as described by Hyatt et al. (2007) except that 125 μl of PrepMan® Ultra Sample Preparation Reagent (Applied Biosystems, Foster City, CA) and 100 mg of zirconium/silica beads (Biospec Products, Bartlesville, OK) were used so that the entire swab was immersed during the procedure and the bead-beating steps were conducted using a FastPrep®-24 homogenizer (MP Biomedicals, Santa Ana, CA). We used a real-time TaqMan PCR for duplex detection of B. salamandrivorans and B. dendrobatidis on the extracted DNA as described in Blooi et al. (2013, 2016). We ran reactions on the 7500 fast real-time PCR system (Applied Biosystems, Foster City, CA) using QuantiFast Probe RT-PCR mastermix kit with ROX dye (Qiagen, Valencia, CA) and BSA as per the kit instructions. We used five microliters of the PrepMan® solution containing the extracted DNA as template for the PCR. We included a negative extraction control and a standard curve run in duplicate on each PCR plate. The standard curve consisted of five different concentrations of the target sequence for B. salamandrivorans or B. dendrobatidis inserted into plasmids. The concentrations of the standards occurred at ten-fold dilutions ranging from 110-1,100,000 copies (0.5 fg – 5000 fg DNA) per reaction (on some initial runs, the standard range was 11-110,000 copies per reaction). The threshold for signal detection was set at 5% of the maximum fluorescence of the standards run for that assay. We considered a positive detection of Bsal or Bd DNA if there was a detectable signal at 40 or fewer PCR cycles and no detection in all other cases. We calculated the efficiency of each run using standard curve amplification and repeated PCR plates with an efficiency of less than 90% or greater than 110%. 20180530 Daniel A Grear USGS - MIDCONTINENT REGION mailing and physical

National Wildlife Health Ctr,NWHC - Main Building

Madison WI 53711 608-270-2478 dgrear@usgs.gov BsalBdDataFull.csv Comma Separated Value (CSV) file containing data. Producer Defined SwabID Barcode or submitter-assigned identification number Producer Defined Most of these values are 10 digits and correspond to the barcode placed on vials that contained the swabs. CollectionDate Date of field swab collection Producer Defined NA No Data Producer defined The dates are recorded in the format MM-DD-YYYY, e.g. 2-15-2017 is February 15, 2017. Six samples from Missouri are missing TaxaID Numeric code for species corresponding to the Integrated Taxonomic Information System (ITIS) found at https://www.itis.gov/ Integrated Taxonomic Information System (ITIS) 173446 775939 ScientificName The species name that corresponds to the ITIS TaxaID in previous column Producer Defined NA No Data Producer defined The species name in this field consists of the genus and specific epithet that correspond to the ITIS TaxaID SiteName Submitter-assigned site name where swabs were collected Producer Defined NA No Data Producer defined This is the name of the site assigned by the submitter and used in the Amphibian Reasearch and Monitoring Initiative (ARMI) database. In many instances the site name is the known name of the particular public place, but other site names may be made up by the individual submitter. State The full name of the state in which the swab was collected is given. Producer Defined The full name of the state in which the swab was collected is given. County TheThe county (or parish in Louisiana) in which the swab was collected is given county (or parish in Louisiana) in which the swab was collected is given Producer Defined The full name of the state in which the swab was collected is given. Latitude The degrees latitude of the site where swabs were collected (WGS84). For two records of a sensitive species, locality is withheld and is denoted as NA. Producer Defined NA No Data Producer defined 26.60217 48.98806 degrees Longitude The degrees longitude of the site where swabs were collected (WGS84). For two records of a sensitive species, locality is withheld and is denoted as NA. Producer Defined NA No Data Producer defined -124.09576 -71.4883 degrees Elevation_m The elevation of the site where swabs were collected. An NA is denoted where elevation was not recorded. Producer Defined NA No Data Producer defined 0 3265 meters Sex The sex of the individual swabbed Producer Defined NA No Data Producer defined Male Sex was determined to be male Producer defined Female Sex was determined to be female Producer defined Unknown Sex was not determined due to age or lack of secondary sexual characteristics. Producer defined Age The age of the swabbed individual. An NA is given when age was not ascertained. Producer Defined NA No Data Producer defined Adult Age was determined to be adult. Producer defined Juvenile Age was determined to be juvenile. Producer defined Larval Age was determined to be larval. Producer defined BodyLength The body length of the swabbed individual. For amphibians, this is the straight-line distance from the tip of the snout to the vent. It does not include the tail length. An NA is denoted for those records where body length was not measured. Producer Defined NA No Data Producer defined 6 622 millimeters CaptureEnvironment The habitat in which the swabbed animal was captured Producer Defined aquatic Captured in aquatic (water) habitats Producer defined terrestrial Captured in terrestrial (land) habitats Producer defined Bsal.Result binary result for Bsal PCR, positive = detected, negative = not detected Producer Defined negative Swab determined to be negative for Bsal Producer defined Case Internal lab identifier for group of samples received in the same shipment Producer Defined NA No Data Producer defined 27063 28406 integer > 0 Acc Internal lab identifier for individual sample within a Case Producer Defined NA No Data Producer defined 1 446 integer > 0 Bdct Cycle threshold value from PCR reaction (max=40). Lower values indicate more target DNA in sample. Measured at the PCR cycle where a signal was detected at 5% of max fluorescence and subsequent signal followed a true amplification curve. NA = no signal detected; No Amp = signal detected, but due to signal noise and not amplification of the target; equivocal = signal detected during last cycle with likely amplification, but reaction cycles stopped before true amplification could be determined. Producer Defined NA not observed Producer defined 9.81 40.0 positive continuous value minimum = 0, maximum = 40, not observed = NA Bd.Result Interpretation of quantitative PCR results. Positive = true amplification signal detected; Negative = no fluorescent signal detected; No Amp = signal detected, but due to signal noise and not amplification of the target. Manually confirmed during lab QA/QC; equivocal = signal detected during last cycle with likely amplification, but reaction cycles stopped before true amplification could be determined. Producer Defined NA No Data Producer defined positive true amplification signal detected Producer defined negative no fluorescent signal detected Producer defined copyNum Calculated estimate of the number of target DNA copies per sample Producer Defined NA No Data Producer defined 0.0 1209654620.10164 positive continuous value Gesp Genus and species used to estimate phylogenetic distance authority NA No Data Producer defined Anuran taxonomy according to Matzke, K. Iizuka, M. S. Min, S. L. Kuzmin, Y. P. Zhang, D. C. Cannatella, D. M. Hillis, and J. Che. 2016. Spatiotemporal diversification of the true frogs (Genus Rana): A historical framework for a widely studied group of model organisms. Systematic Biology 65:824–842., Caudate taxonomy according to, Pyron A, Wiens JJ. 2011 A large-scale phylogeny of Amphibia with over 2,800 species, and a revised classification of extant frogs, salamanders, and caecilians. Mol Phy Evol 61: 543-583. order taxonomic order authority NA No Data Producer defined Caudata Member of the order caudata, commonly known as the salamanders Pyron A, Wiens JJ. 2011 A large-scale phylogeny of Amphibia with over 2,800 species, and a revised classification of extant frogs, salamanders, and caecilians. Mol Phy Evol 61: 543-583. Anura Member of the order anura, commonly known as the frogs and toads Pyron A, Wiens JJ. 2011 A large-scale phylogeny of Amphibia with over 2,800 species, and a revised classification of extant frogs, salamanders, and caecilians. Mol Phy Evol 61: 543-583. siteID numeric index that groups samples within 0.2km to a site Producer Defined NA No Data Producer defined 1 593 integer > 0 siteVID numeric index for site visits that occurred greater than 5 days apart at each site Producer Defined NA No Data Producer defined 1 692 integer > 0 reg NOAA climate region where the site was sampled authority NA no site location available Producer defined SE Southeast Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. SW Southwest Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. S South Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. W West Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. NE Northeast Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. OV Ohio Valley Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. UM Upper Midwest Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. NRP Northern Rockies and Plains Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. NW Northwest Karl, T. R., and J. Kloss. 1984. Regional and national monthly, seasonal, and annual temperature weighted by area 1895-1983. Historical Climatology Series 4–3:38. KGzone 3rd level Köppen-Geiger climate zone where the sample was collected authority NA no site location available Producer defined Cfa Temperate, No Dry season, Hot summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. BSk Dry, Semi-Arid, Cold Producer defined BSh Dry, Semi-Arid Hot Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. Csb Temperate, Dry Summer, warm summer Producer defined Csa Temperate, dry summer, hot summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. BWh Dry, Arid, Hot Producer defined Dfb Continental, No dry season, warm summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. Dfc Continental, No Dry season, cold summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. Dfa Continental, No Dry season, hot summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. Cfb Temperate, No Dry season, warm summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. KG5 2nd level Köppen-Geiger climate zone where the sample was collected authority NA no site location available Producer defined Cf Temperate No Dry Season Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. BS Dry, Semi-arid Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. Cs Temperate, Dry summer Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. BW Dry, Arid desert Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. Df Continental, No Dry Season Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. KG3 1st level Köppen-Geiger climate zone where the sample was collected authority NA no site location available Producer defined C Temperate Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. B Dry Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. D Continental Köppen, W., E. Volken, and S. Brönnimann. 2011. The thermal zones of the Earth according to the duration of hot, moderate and cold periods and to the impact of heat on the organic world. Meteorologische Zeitschrift 20:351–360. m1max maximum daily temperature estimated at the day of sampling Producer Defined NA site location or date not recorded Producer defined -2.12100005149841 41.4440002441406 degrees Celsius m7max average maximum daily temperature estimated for the 7 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -4.3361428124564 37.9541435241699 degrees Celsius m10max average maximum daily temperature estimated for the 10 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -4.76909995079041 37.5731006622314 degrees Celsius m20max average maximum daily temperature estimated for the 20 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -1.23154997825623 36.6817002296448 degrees Celsius m30max average maximum daily temperature estimated for the 30 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -0.379466656843821 36.8477333704631 degrees Celsius m40max average maximum daily temperature estimated for the 40 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.48657501488924 35.7372999191284 degrees Celsius m50max average maximum daily temperature estimated for the 50 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.270380011945963 35.505479888916 degrees Celsius m60max average maximum daily temperature estimated for the 60 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -1.23033336053292 35.2114498774211 degrees Celsius m70max average maximum daily temperature estimated for the 70 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -1.78474287773882 34.8333427701678 degrees Celsius m80max average maximum daily temperature estimated for the 80 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -1.91463751154952 33.9648499488831 degrees Celsius m90max average maximum daily temperature estimated for the 90 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -1.404355569308 33.4265222549438 degrees Celsius m100max average maximum daily temperature estimated for the 100 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -0.750490018986166 33.201130065918 degrees Celsius m110max average maximum daily temperature estimated for the 110 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -0.590272747488184 32.8168909593062 degrees Celsius m120max average maximum daily temperature estimated for the 120 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.068949979947259 32.445316696167 degrees Celsius m1min minimum daily temperature estimated at the day of sampling Producer Defined NA site location or date not recorded Producer defined -11.0030002593994 25.3959999084473 degrees Celsius m7min average minimum daily temperature estimated for the 7 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -14.4977144513811 25.9345716748919 degrees Celsius m10min average minimum daily temperature estimated for the 10 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -14.6392001152039 25.2576002120972 degrees Celsius m20min average minimum daily temperature estimated for the 20 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -12.010050034523 25.2548999786377 degrees Celsius m30min average minimum daily temperature estimated for the 30 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -11.1107666889826 25.0179000854492 degrees Celsius m40min average minimum daily temperature estimated for the 40 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -9.89960002303124 25.0793500900269 degrees Celsius m50min average minimum daily temperature estimated for the 50 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -10.0703800296783 25.0300600814819 degrees Celsius m60min average minimum daily temperature estimated for the 60 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -9.63909999827544 24.9606167475382 degrees Celsius m70min average minimum daily temperature estimated for the 70 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -10.134142854384 25.1061286381313 degrees Celsius m80min average minimum daily temperature estimated for the 80 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -9.97512500211596 24.9785000801086 degrees Celsius m90min average minimum daily temperature estimated for the 90 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -9.37382222182221 24.8689222759671 degrees Celsius m100min average minimum daily temperature estimated for the 100 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -8.72084999567829 24.7487600517273 degrees Celsius m110min average minimum daily temperature estimated for the 110 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -8.43286363000728 24.5233000321822 degrees Celsius m120min average minimum daily temperature estimated for the 120 days preceding sampling Producer Defined NA site location or date not recorded Producer defined -8.28559162636908 24.2375416596731 degrees Celsius t1precip total precipitation estimated on the day of sampling Producer Defined NA site location or date not recorded Producer defined 0.0 53.1570014953613 millimeters t7precip total precipitation estimated over the 7 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 211.193001445383 millimeters t10precip total precipitation estimated over the 10 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 211.193001445383 millimeters t20precip total precipitation estimated over the 20 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 388.180000789464 millimeters t30precip total precipitation estimated over the 30 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 449.148995749652 millimeters t40precip total precipitation estimated over the 40 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 530.477995090187 millimeters t50precip total precipitation estimated over the 50 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 603.063001116621 millimeters t60precip total precipitation estimated over the 60 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 806.481998801231 millimeters t70precip total precipitation estimated over the 70 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 998.462995380163 millimeters t80precip total precipitation estimated over the 80 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 1049.34500417113 millimeters t90precip total precipitation estimated over the 90 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 1440.03400170803 millimeters t100precip total precipitation estimated over the 100 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 1557.1929987669 millimeters t110precip total precipitation estimated over the 110 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.0 1689.18400156498 millimeters t120precip total precipitation estimated over the 120 days preceding sampling Producer Defined NA site location or date not recorded Producer defined 0.384999997913837 1892.12900030613 millimeters U.S. Geological Survey - ScienceBase mailing and physical

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