Christopher R. Dawald Robert M. Burdis 20180214 spreadsheet Online U.S. Geological Survey https://doi.org/10.5066/F7445KR1 Christopher R. Dawald Robert M. Burdis 2017 A completion report submitted to the U.S. Army Corps of Engineers’ Upper Mississippi River Restoration Program from the U.S. Geological Survey, 2015D15. U.S. Army Corps of Engineers’ Upper Mississippi River Restoration Program U.S. Army Corps of Engineers’ Upper Mississippi River Restoration Program N/A Limited information is known regarding the rotifer community on the Upper Mississippi River (UMR). Studies have shown that rotifers are a common prey for invasive bighead carp (Hypophthalmichthys nobilis) and silver carp (H. molitrix) as well as native fishes of the UMR and there is concern these invasive carp will compete with native fishes for food resources. This study was conducted to provide baseline rotifer community data before range expansion of these invasive carps into the upper reaches of the UMR. Vertical tows were taken to collect rotifer samples over a four year period in Lake Pepin, a natural riverine lake in Pool 4 of the UMR. Four dominant genera (Keratella, Polyarthra, Trichocerca and Brachionus) comprised the samples and significant correlations between rotifer abundance and temperature, volatile suspended solids and chlorophyll-a were observed. Our results identify potential drivers of rotifer abundance and provide robust rotifer abundance and composition data. These pre-invasion data are critical to recognizing the effects invasive carp have on zooplankton communities and the UMR ecosystem. The purpose of the data was to provide pre-invasion of silver carp and bighead carp abundance and composition of the rotifer community of the Upper Mississippi River. These pre-invasion data are critical to recognizing the effects invasive carp have on zooplankton communities and the UMR ecosystem. 20120503 20151007 observed Complete None planned Lake Pepin -92.546081542969 -92.062683105471 44.581664700316 44.315005310244 ISO 19115 Topic Category biota None Rotifers Lake Pepin zooplankton invasive carp USGS Metadata Identifier USGS:5a842ee2e4b00f54eb33f867 None Lake Pepin Upper Mississippi River Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Platyzoa Phylum Rotifera rotifers wheel animalcules Class Monogonta Subclass Monogononta Superorder Pseudotrocha Order Ploima Family Asplanchnidae Genus Asplanchna TSN: 59235 Genus Harringia TSN: 59251 Family Brachionidae Genus Brachionus TSN: 58434 Genus Keratella TSN: 58348 Genus Notholca TSN: 58396 Genus Anuraeopsis TSN: 58525 Genus Kellicottia TSN: 58485 Family Notommatidae Genus Cephalodella TSN: 58819 Family Gastropodidae Genus Ascomorpha TSN: 59167 Family Dicranophoridae Genus Encentrum TSN: 58784 Family Euchlanidae Genus Euchlanis TSN: 58578 Family Lecanidae Genus Lecane TSN: 58634 Genus Monostyla TSN: 58747 Family Lepadellidae Genus Lepadella TSN: 58488 Family Mytilinidae Genus Mytilina TSN: 58546 Family Synchaetidae Genus Polyarthra TSN: 59270 Genus Synchaeta TSN: 59255 Family Trichocercidae Genus Trichocerca TSN: 59074 Family Trichotriidae Genus Trichotria TSN: 58564 Superorder Gnesiotrocha Order Flosculariaceae Family Conochilidae Genus Conochilus TSN: 59417 Family Filiniidae Genus Filinia TSN: 59425 Family Testudinellidae Genus Testudinella TSN: 59297 Class Bdelloidea Subclass Bdelloidea TSN: 58246 None. Users are advised to read the data set's metadata thoroughly to understand appropriate use and data limitations. none Robert M. Burdis Minnesota Department of Natural Resources Water Quality Specialist mailing and physical

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Lake City Minnesota 55041 United States 651-345-3331 x224 Robert.Burdis@state.mn.us We would like to give a special thanks to Jodie Hirsch for helping with rotifer identification. We would also like to thank the numerous interns and co-workers for their help collecting rotifer samples. Funding for this project was provided by the U.S. Army Corps of Engineers’ Upper Mississippi River Restoration (UMRR) program, Long Term Resource Monitoring (LTRM) element in collaboration with the Minnesota Department of Natural Resources. Microsoft Windows 7 operating system comma separated value format exported from SAS. Soballe, D. M. Fischer, J. R. 2004 La Crosse (WI) U.S. Geological Survey, Upper Midwest Environmental Sciences Center https://www.umesc.usgs.gov/documents/reports/2004/04t00201.pdf Burdis, R. M. Hoxmeier, R. J. 2011 Hydrobiologia Hydrobiologia Chick, J. H. Levchuk, A. P. Medley, K. A. Havel, J. H. 2010 Limnology and Oceanography: Methods Limnology and Oceanography: Methods No formal attribute accuracy tests were conducted. No formal logical accuracy tests were conducted Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. A formal accuracy assessment of the horizontal positional information in the data set has not been conducted. A formal accuracy assessment of the vertical positional information in the data set has either not been conducted, or is not applicable. both water was collected in the field and examined in the lab Soballe, D. M. Fischer, J. R. 2004 publication. Technical Report: no. LTRMP2004-T002-1 https://www.umesc.usgs.gov/documents/reports/2004/04t00201.pdf Burdis, R. M. Hoxmeier, R. J. 2011 publication. Hydrobiologia, 667(1), 69-87 https://doi.org/10.1007/s10750-011-0639-y Chick, J. H. Levchuk, A. P. Medley, K. A. Havel, J. H. 2010 publication, Limnology and Oceanography: Methods 8(3), 79-87 10.4319/lom.2010.8.0079 Upper Mississippi River Restoration Program-Long Term Resource Monitoring Element 1998 application/service Online USGS https://www.umesc.usgs.gov/data_library/water_quality/water1_query.shtml Digital and/or Hardcopy 19880101 20170801 observed UMRR-LTRM Water Quality Monitoring Water quality data collected at the fixed sites was part of the Long Term Resource Monitoring's sampling effort. United States Corps of Engineers St. Paul District 1959 tabular digital data St Paul, MN USACOE http://www.mvp-wc.usace.army.mil/data/LockDam_03.Data.html Digital and/or Hardcopy 19590101 20171127 observed Lock and Dam 3 Flow Data Ten day average before sampling date of the lock and dam 3 flow data, from the field Flow-Out of the one day flow summaries. Rotifer samples were collected during routine water quality sampling for the Upper Mississippi River Restoration Program’s, Long Term Resource Monitoring (LTRM) element. Samples were collected at four LTRM fixed sites (Soballe & Fischer, 2004) in Lake Pepin. Samples were taken biweekly from May through August and monthly in September and October from 2012 to 2015. Samples were collected with a plankton net (20 µm mesh, 20 cm diameter) towed vertically starting one meter above the substrate. The length of tow was recorded in order to calculate sample volume. A 20 µm mesh was chosen because coarser nets were shown to underestimate rotifer abundance (Chick et al., 2010). Samples were preserved with 95% reagent alcohol. A suite of limnological variables were also collected at each site as part of the routine LTRM water quality monitoring effort (Soballe & Fischer, 2004). 20171101 Samples were brought to a volume of 40 to 70 mL with distilled water. A Hensen Stemple pipette was used to place aliquots (1 mL) into a gridded Sedgewick Rafter cell. Rotifer densities were calculated using counts and sample volumes. At least 100 rotifers were enumerated per sample, replicating methods from a previous zooplankton study in Pool 4 of the UMR (Burdis & Hoxmeier, 2011). A combination of Eosin-B and Biebrich scarlet was used to stain the samples for easier identification. Specimens were viewed under a compound microscope at 100 to 200X magnification. Identification was completed to genus, except that Bdelloidea were only recorded to class. 20171101 Vector G-polygon 1114 Universal Transverse Mercator 15 0.9996 -93.0 0.0 500000.0 0.0 coordinate pair 0.6096 0.6096 North_American_Datum_1927 Clarke_1866 6378206.4 294.9786982 Rotifer.csv Comma Separated Value (CSV) file containing data. Producer defined Date Sample Date Producer defined 20120503 20151007 Site Site identification number. Sites use the annn.na format where the leading a designates the river (M = Mississippi and I = Illinois), nnn.n is the river mile (recorded to the nearest 0.1 mile), and the trailing a is an alphabetic code for the relative lateral position across the floodplain Producer defined 766 LTRM water quality fixed site M766.0I Producer defined 771 LTRM water quality fixed site M771.2P Producer defined 775 LTRM water quality fixed site M775.6Q Producer defined 781 LTRM water qaulity fixed site M781.2O Producer defined Total_density Total rotifers collected per Liter of water Producer defined 4.8 220.9 number per liter Asplanchna Total Asplanchna per liter of water Producer defined 0.0 7.5 number per liter Brachionus Total Brachionus per liter of water Producer defined 0.0 53.4 number per liter Cephalodella Total Cephalodella per liter of water Producer defined 0.0 0.8 number per liter Ascomorpha Total Ascomorpha per liter of water Producer defined 0.0 21.2 number per liter Conochilus Total Conochilus per liter of water Producer defined 0.0 2.3 number per liter Bdelloidea Total Bdelloidae per liter of water Producer defined 0.0 1.2 number per liter Encentrum Total Encentrum per liter of water Producer defined 0.0 8.9 number per liter Euchlanis Total Euchlanis per liter of water Producer defined 0.0 29.9 number per liter Filinia Total Filinia per liter of water Producer defined 0.0 9.3 number per liter Kellicottia Total Kelicottia per liter of water Producer defined 0.0 1.1 number per liter Keratella Total Keratella per liter of water Producer defined 0.5 125.1 number per liter Lecane Total Lecane per liter of water Producer defined 0.0 0.5 number per liter Lepadella Total Lepadella per liter of water Producer defined 0.0 0.3 number per liter Monostyla Total Monostyla per liter of water Producer defined 0.0 0.5 number per liter Mytilina Total Mytilina per liter of water Producer defined 0.0 0.3 number per liter Notholca Total Notholca per liter of water Producer defined 0.0 1.8 number per liter Polyarthra Total Polyarthra per liter of water Producer defined 0.4 66.2 number per liter Synchaeta Total Synchaeta per liter of water Producer defined 0.0 11.9 number per liter Testudinella Total Testundinella per liter of water Producer defined 0.0 0.1 number per liter Trichocerca Total Trichocerca per liter of water Producer defined 0.0 60.6 number per liter Trichotria Total Trichotria per liter of water Producer defined 0.0 0.5 number per liter Anuraeopsis Total Anuraeopsis per liter of water Producer defined 0.0 11.0 number per liter Harringia Total Harringia per liter of water Producer defined 0.0 6.0 number per liter Flow10day ten day mean discharge at Lock and Dam 3 prior to the sampling date Producer defined 4760.0 108500.0 cubic feet per second ZMAX The vertical distance (to the nearest 0.1 m) between the substrate and the free water surface. Zmax is recorded as zero when the water is frozen to the substrate (no free water surface exists or cannot be reliably determined). Producer defined 4.6 11.2 meter SECCHI The vertical distance below the water surface at which a secchi disk disappears from view. Recorded in centimeters. Producer defined 22 276 centimeters Z The vertical distance (to the nearest 0.1m) between the free water surface and the point of sample collection or ambient measurement. When ice is present, the following conventions are used to record Z: (1) samples collected more than 25 cm below the bottom of the ice are assigned a z value in the usual manner as described above, (2) samples collected 16-25 cm below the ice are assigned a z value of 0.2 m. (3) Samples collected between 0 and 14 cm below the ice are assigned a z value of 0.1m, and (4) samples collected above the lower level of the ice (i.e., in the access hole) are assigned a z value of zero. Producer defined 0.2 0.2 meter TEMP Water temperature for the sample measured in degrees Celsius Producer defined 12.8 31.8 degrees celsius DO Amount of Dissolved Oxygen in the sample. Measured in milligrams/liter Producer defined 5.7 15.2 mg/L TURB Turbidity (water clarity) measurement for the sample. Measured in nephelometric turbidity to the nearest whole nephelometric techniques Producer defined 2 37 nephelometric COND Conductivity (ability of the water to conduct an electric current) measurement for the sample. Measured to the nearest uS/cm Producer defined 300 578 uS/cm SS The total particulate material trapped by a glass fiber filter and dried at 105 degrees Centigrade. Reported as milligrams per liter Producer defined 2.0 35.6 milligrams per liter VSS The weight loss on combustion at 500 - 500 degrees Centigrade of the total suspended solids residue for the sample. Reported in milligrams per liter. Producer defined 2.3 22.44 milligrams per liter CHLF Uncalibrated fluorometric chlorophyll. Measured in micrograms per liter Producer defined 1.6 71.6 micrograms per liter U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. 20220623 Benjamin A Schlifer U.S. Geological Survey It Specialist (Datamgt) mailing and physical

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La Crosse WI 54603 United States 608-781-6359 608-783-6066 bschlifer@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999 Record created using USGS Metadata Wizard tool. (https://github.com/usgs/fort-pymdwizard)