Erin Muths Blake Hossack David Pilliod 2017 tabular digital data https://doi.org/10.5066/F7MW2G32 These data detail the presence of disease (Bd, Batrachochytrium dendrobatidis) at a set of sites that are associated with the project detailed in the abstract below: Amphibian decline is a problem of global importance, with over 40% of species considered at risk. This phenomenon is not limited to the tropics or to other countries. Amphibian species in the U.S. are also declining, contributing to the larger, global phenomenon. For example, in the State of Wyoming, the Wyoming toad has been extirpated in the wild and the boreal toad is a species of special concern. Understanding biotic and abiotic factors that influence amphibian persistence is critical for amphibian conservation. This work in northern Wyoming has focused on demography, habitat alteration and creation, and disease in the context of multiple amphibian populations. One of the foci has been to identify the capacity for mitigation wetlands (those created to offset losses due to, for example, road construction) to serve as habitat for amphibians. Four species of amphibians native to Wyoming, including the boreal toad, reside in this region. Our previous research indicates that the toad population at Blackrock is declining at 5-6% per year and that disease due to the amphibian chytrid fungus is contributing to this decline. Our demographic work at this site began in 2003, focusing solely on the boreal toad. Additional funding in 2012 allowed us to increase the scope of the project and assess chorus frog, salamander and Columbia spotted frog populations, invertebrate assemblages, work to quantify the use of mitigation sites by amphibians, and to expand efforts to include sites on Togwotee Pass a short distance away from Blackrock. Because most previous studies of amphibian use of created wetlands have taken place in the eastern United States, this project, incorporating demographic and disease dynamics as well as community composition and mitigation effects of created wetlands, is unique and provides a case study in the Intermountain West. By 2015, all four native amphibian species were observed at one of the created wetlands, and all of them, including the boreal toad, were breeding (evidenced by breeding behavior, eggs or tadpoles). Data were collected to inform conservation and management of amphibian populations. Information will be used to understand demographics of natural populations (survival, recruitment, and population growth rate), disease, and occupancy of amphibians across a landscape. Additional site specific data such as invertebrate species, calls of other organisms (i.e. birds), and vegetation will inform the implementation of mitigation (e.g. as required when amphibian habitat is destroyed during road construction, or possibly the creation of new amphibian-specific sites for reintroduction). 20150425 20160725 ground condition Complete None planned -110.1482391357422 -109.98550415039064 43.79885402720353 43.69667260550321 Togwotee and Blackrock wetlands ISO 19115 Topic Category biota None wetland ecosystems amphibians USGS Metadata Identifier USGS:59f9f2b9e4b0531197aff072 None Blackrock Togwotee None. Please see 'Distribution Info' for details. None. Users are advised to read the data set's metadata thoroughly to understand appropriate use and data limitations. Erin L Muths U.S. Geological Survey, Southwest Region Research Zoologist mailing address

2150 Centre Avenue Bldg C

Fort Collins CO 80526 United States 970-226-9474 970-226-9230 muthse@usgs.gov No formal attribute accuracy tests were conducted. No formal logical accuracy tests were conducted. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. A formal accuracy assessment of the horizontal positional information in the data set has not been conducted. A formal accuracy assessment of the vertical positional information in the data set has either not been conducted, or is not applicable. We collected skin swabs from up to 30 amphibians of each species at selected sites each season. Swabs were collected and assessed for DNA from Bd per Boyle, D. G., D. B. Boyle, V. Olsen, J. A. T. Morgan, and A. D. Hyatt. Diseases of aquatic organisms 60, no. 2 (2004): 141-148. We noted the number of animals swabbed to test for Bd and the number of positive animals (from lab analysis) each year. Data were quality checked at the end of each year. There are no data for 2014 due to unforeseen field circumstances.Data were quality checked at the end of each year to be certain that the appropriate number of swabs was assigned to each species and each site. 2017 Blackrock_Bd_datarelease.csv Comma Separate Value (CSV) file containing data. producer defined Species kind of species tested Producer defined kind of species tested - Latin name bufo boreas = boreal toad ambystoma tigrinum = tiger salamander pseudacris maculata = boreal chorus frog rana lutieventris = Columbia spotted frog Year year samples collected Producer defined 2012 2015 No. Tested number of amphibians swabbed to test for Bd using DNA analysis Producer defined 1 164 No. Positive number of amphibians that tested positive for the presence of Bd Producer defined 0 84 No. Negative number of amphibians that tested negative for the presence of Bd Producer defined 1 80 % naive prevalence: the percent of animals tested that were positive for Bd. For example, the number testing positive divided by the total number tested. Producer defined 0.0 0.83 GS ScienceBase U.S. Geological Survey mailing address

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 United States 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Digital Data https://doi.org/10.5066/F7MW2G32 None 20200820 Erin L Muths U.S. Geological Survey, Southwest Region Research Zoologist mailing address

2150 Centre Avenue Bldg C

Fort Collins CO 80526 United States 970-226-9474 970-226-9230 muthse@usgs.gov FGDC Biological Data Profile of the CDGSM FGDC-STD-001.1-1999 Record created using USGS Metadata Wizard tool. (https://github.com/usgs/fort-pymdwizard)