Jo Ellen Hinck Danielle Cleveland 20161109 Tabular Digital Data, CSV format on line ScienceBase https://doi.org/10.5066/F7QF8R16 Jo Ellen Hinck Danielle Cleveland 20170113 189:56 Publication (Journal) Unknown Environmental Monitoring and Assessment https://doi.org/10.1007/s10661-017-5756-1 The risks to wildlife and humans from uranium (U) mining to the Grand Canyon watershed are largely unknown. In addition to U, other co-occurring ore constituents contribute to risks to biological receptors depending on their toxicological profiles. This data was collected to characterize the pre-mining concentrations of total arsenic (As), cadmium (Cd), copper (Cu), lead (Pb), mercury (Hg), nickel (Ni), selenium (Se), thallium (Tl), U, and zinc (Zn); radiation levels; and histopathologies in biota (vegetation, invertebrates, amphibians, birds, and mammals) at the Canyon Mine. This data was collected to characterize the pre-mining concentrations of radiation levels in biota (vegetation, invertebrates, amphibians, birds, and mammals) at the Canyon Mine. 20140101 20150201 ground condition Complete Not planned None uranium mining chemistry USGS Thesaurus uranium Marine Realms Information Bank (MRIB) keywords biological resources USGS Metadata Identifier USGS:58c30882e4b0f37a93ed91a7 Common geographic areas Grand Canyon none None. Users are advised to read the data set's metadata thoroughly to understand appropriate use and data limitations. Jo Ellen Hinck U.S. Geological Survey mailing and physical

4200 New Haven Road

Columbia MO 65201 USA 573-876-1808 jhinck@usgs.gov U.S. Fish and Wildlife Service Environment as of Metadata Creation: Microsoft [Unknown] Version 6.2 (Build 9200) ; Esri ArcGIS 10.4.1 (Build 5686) Service Pack N/A (Build N/A) USGS Biocomplexity Thesaurus Mammals None 20161121 Other Unknown Unknown https://www.itis.gov Donald F Hoffmeister 1986 BOOK Tucson, AZ University of Arizona Press Jo Ellen Hinck U.S. Geological Survey mailing and physical

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Columbia MO 65201 USA 573-875-5399 jhinck@usgs.gov expert advice Experts examined and identified the species by consensus. Mammals were identified to genus species. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Mammalia Subclass Theria Infraclass Eutheria Order Rodentia Suborder Myomorpha Superfamily Muroidea Family Cricetidae Subfamily Neotominae Genus Peromyscus Species Peromyscus maniculatus Ratón norteamericano souris sylvestre Deer Mouse North American Deermouse Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Mammalia Subclass Theria Infraclass Eutheria Order Rodentia Suborder Sciuromorpha Family Sciuridae Subfamily Xerinae Tribe Marmotini Genus Tamias Subgenus Tamias (Neotamias) Species Tamias dorsalis Cliff Chipmunk Subspecies Tamias dorsalis carminis Subspecies Tamias dorsalis dorsalis Subspecies Tamias dorsalis grinnelli Subspecies Tamias dorsalis nidoensis Subspecies Tamias dorsalis sonoriensis Subspecies Tamias dorsalis utahensis Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Mammalia Subclass Theria Infraclass Eutheria Order Rodentia Suborder Castorimorpha Family Geomyidae Genus Thomomys Subgenus Thomomys (Megascapheus) Species Thomomys bottae Tuza de Botta Botta's Pocket Gopher Subspecies Thomomys bottae abbotti Subspecies Thomomys bottae abstrusus Subspecies Thomomys bottae actuosus Subspecies Thomomys bottae albatus Subspecies Thomomys bottae albicaudatus Subspecies Thomomys bottae alexandrae Subspecies Thomomys bottae alpinus Subspecies Thomomys bottae alticolus Subspecies Thomomys bottae analogus Subspecies Thomomys bottae angustidens Subspecies Thomomys bottae anitae Subspecies Thomomys bottae aphrastus Subspecies Thomomys bottae aureiventris Subspecies Thomomys bottae aureus Subspecies Thomomys bottae awahnee Subspecies Thomomys bottae baileyi Subspecies Thomomys bottae basilicae Subspecies Thomomys bottae birdseyei Subspecies Thomomys bottae bonnevillei Subspecies Thomomys bottae borjasensis Subspecies Thomomys bottae bottae Subspecies Thomomys bottae brazierhowelli Subspecies Thomomys bottae brevidens Subspecies Thomomys bottae cactophilus Subspecies Thomomys bottae camoae Subspecies Thomomys bottae canus Subspecies Thomomys bottae catalinae Subspecies Thomomys bottae catavinensis Subspecies Thomomys bottae centralis Subspecies Thomomys bottae cervinus Subspecies Thomomys bottae chrysonotus Subspecies Thomomys bottae cinereus Subspecies Thomomys bottae collis Subspecies Thomomys bottae concisor Subspecies Thomomys bottae confinalis Subspecies Thomomys bottae connectens Subspecies Thomomys bottae contractus Subspecies Thomomys bottae convergens Subspecies Thomomys bottae convexus Subspecies Thomomys bottae cultellus Subspecies Thomomys bottae cunicularius Subspecies Thomomys bottae curtatus Subspecies Thomomys bottae depressus Subspecies Thomomys bottae desertorum Subspecies Thomomys bottae detumidus Subspecies Thomomys bottae dissimilis Subspecies Thomomys bottae divergens Subspecies Thomomys bottae estanciae Subspecies Thomomys bottae fulvus Subspecies Thomomys bottae fumosus Subspecies Thomomys bottae guadalupensis Subspecies Thomomys bottae homorus Subspecies Thomomys bottae howelli Subspecies Thomomys bottae humilis Subspecies Thomomys bottae internatus Subspecies Thomomys bottae jojobae Subspecies Thomomys bottae juarezensis Subspecies Thomomys bottae lachuguilla Subspecies Thomomys bottae lacrymalis Subspecies Thomomys bottae laticeps Subspecies Thomomys bottae latus Subspecies Thomomys bottae lenis Subspecies Thomomys bottae leucodon Subspecies Thomomys bottae levidensis Subspecies Thomomys bottae limitaris Subspecies Thomomys bottae limpiae Subspecies Thomomys bottae lucidus Subspecies Thomomys bottae lucrificus Subspecies Thomomys bottae martirensis Subspecies Thomomys bottae mearnsi Subspecies Thomomys bottae mewa Subspecies Thomomys bottae minimus Subspecies Thomomys bottae modicus Subspecies Thomomys bottae morulus Subspecies Thomomys bottae nanus Subspecies Thomomys bottae navus Subspecies Thomomys bottae nesophilus Subspecies Thomomys bottae nigricans Subspecies Thomomys bottae operarius Subspecies Thomomys bottae optabilis Subspecies Thomomys bottae opulentus Subspecies Thomomys bottae osgoodi Subspecies Thomomys bottae paguatae Subspecies Thomomys bottae pascalis Subspecies Thomomys bottae pectoralis Subspecies Thomomys bottae peramplus Subspecies Thomomys bottae perditus Subspecies Thomomys bottae perpallidus Subspecies Thomomys bottae pervagus Subspecies Thomomys bottae pervarius Subspecies Thomomys bottae phelleoecus Subspecies Thomomys bottae pinalensis Subspecies Thomomys bottae planirostris Subspecies Thomomys bottae planorum Subspecies Thomomys bottae powelli Subspecies Thomomys bottae proximarinus Subspecies Thomomys bottae pusillus Subspecies Thomomys bottae retractus Subspecies Thomomys bottae rhizophagus Subspecies Thomomys bottae riparius Subspecies Thomomys bottae robustus Subspecies Thomomys bottae rubidus Subspecies Thomomys bottae ruidosae Subspecies Thomomys bottae rupestris Subspecies Thomomys bottae ruricola Subspecies Thomomys bottae russeolus Subspecies Thomomys bottae saxatilis Subspecies Thomomys bottae scotophilus Subspecies Thomomys bottae sevieri Subspecies Thomomys bottae siccovallis Subspecies Thomomys bottae simulus Subspecies Thomomys bottae sinaloae Subspecies Thomomys bottae solitarius Subspecies Thomomys bottae spatiosus Subspecies Thomomys bottae stansburyi Subspecies Thomomys bottae sturgisi Subspecies Thomomys bottae subsimilis Subspecies Thomomys bottae texensis Subspecies Thomomys bottae tivius Subspecies Thomomys bottae toltecus Subspecies Thomomys bottae tularosae Subspecies Thomomys bottae vanrosseni Subspecies Thomomys bottae varus Subspecies Thomomys bottae vescus Subspecies Thomomys bottae villai Subspecies Thomomys bottae wahwahensis Subspecies Thomomys bottae winthropi Subspecies Thomomys bottae xerophilus No formal attribute accuracy tests were conducted No formal logical accuracy tests were conducted Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted No formal positional accuracy tests were conducted Database was created using data collected in the laboratory. 20161109 Lab Sub-samples of vegetation and rodents were selected for radiochemical analyses; invertebrate masses were too small to complete chemical and radiochemical analyses. Samples were screened for gross alpha and beta radioactivity using gas flow proportional counting (USEPA 1986). Daughter products in the U-238/radium (Ra) series and U-235/actinium (Ac) series decay chains were monitored by gamma spectroscopy (USDOE 1997) as a cost-effective characterization of the site in terms of (1) the presence of radionuclides to ascertain natural or extracted ore states, and (2) the presence of daughter products for which the radiation dose associated with gamma emissions may pose a health risk. Quality control measures for radiochemical analyses included duplicate analyses, laboratory control samples, method blanks, matrix spikes, and matrix spike duplicates. Reporting limits were 4 pCi/g for gross alpha and 10 pCi/g for gross beta. The minimum detectable level (pCi/g) ranges for gamma spectroscopy were as follows: Ac-227, 1.92-20.8; bismuth (Bi)-211, 1.42-14.0; Bi-214, 0.38-7.06; Pb-210, 5.83-383; Pb-211, 5.54-77.3; Pb-214, 0.57-7.82; protactinium (Pa)-231, 6.71-72.6; Pa-234, 43.1-462; Ra-223, 4.72-51.2; Ra-226, 0.66-7.06; thorium (Th)-227, 1.92-20.8; Th-231, 4.72-51.2; Th-234, 5.40-69.5; U-235, 1.32-12.5; and U-238, 5.40-69.5. EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Thomas W. May, 1998. Inspection, Clalibration, Maintenance, and Documentation of hte Mettler PJ-300 Electronic Top-Loading Balance, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab James Zajicek, 1997. Procedure for the Homogenization of Large, Frozen, Biological Samples (Primarily National Contaminant Biomonitoring Program Whole-Body Fish Samples) Using the Hobart Band Saw and Food Grinder, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Thomas W. May, 2004. Sample Transmittal, Receipt and Inventory, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Thomas W. May, 1998. Homogenization of Biological Tissue and Sediments by Brittle Fracture, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Jesse W. Arms, 2007. Mechanical Grinding of Dried or Semi-dried Tissue Samples with the Bamix Mixer/Blender, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Jesse W. Arms, 2005. Homogenization of Samples by Manual Procedures, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Jesse W. Arms, 1998. Sample Storage and Disposal Protocol, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Thomas W. May, 1998. Lyophilization and Percent Moisture in Solid Samples, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Lab Thomas W. May, 1998. Sample Management and Tracking, Uncitable Document, Columbia Environmental Research Center EPA. EMSL 1980 Document None EML, DOE (EML is currently part of the DHS) 1997 Document None Radiation data in support of manuscript "Pre-mining tissue chemistry, radiation, and histopathology data for biota from Canyon Mine" Table containing radiological measurements of flora and mammals collected at Canyon Mine. Producer defined CERC# Analytical laboratory tracking number Producer defined Analytical laboratory tracking number uniquely identifies the sample Receptor Type of biota from which the radiological measurement as collected Producer defined Mammal The sample from which the radiological measurement was collected was a mammal Producer defined Vegetation The sample from which the radiological measurement was collected was a plant Producer defined Site Locale where the sample was collected Producer defined Name of the location were the sample was collected, Canyon Mine breccia pipe mine (35°52’57.50” N, 112°05’44.52” W) is located in the Kaibab National Forest, approximately 10 km south of Tusayan, Arizona. Area An alpha numeric indicator of the area within the site were the sample was collected Producer defined An alpha numeric indicator of the area within the site were the sample was collected Sample Type A description of the sample analytically processed Producer defined Mulit-species composite Sample consists of many species combined into a single sample Producer defined Wholebody Sample consists of the whole body of the organsim collected Producer defined Species Genus and species of the organism collected Producer defined Genus and species of the organism collected, mutispecies composite have value of NA because a common name is not applicable. Common Name Common name of the organism collected Producer defined Common name of the organism collected, mutispecies composite have value of NA because a common name is not applicable. Gross alpha Gross alpha radiation measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Gross beta Gross beta radiation measured in the sample recorded in picocuries per gram Producer defined 10.1 43.5 pCi/g ND Not detected above the detection limit Producer defined Ac_227 Actinium-227 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Bi_211 Bismuth-211 levels in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Bi_214 Bismuth-214 levels in picocuries per gram Producer defined 6.69 6.69 pBi-214/g ND Not detected above the detection limit Producer defined Pb_210 Lead-210 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Pb_211 Lead-211 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Pb_214 Lead-214 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Pa_231 Protactinium-231 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Pa_234m Protactinium-234m levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Ra_223 Radium-223 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Ra_226 Radium-226 levels measured in the sample recorded in picocuries per gram Producer defined 6.69 6.69 pRa-226/g ND Not detected above the detection limit Producer defined Th_227 Thorium-227 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Th_231 Thorium-231 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined Th_234 Thorium-234 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined U_235 Uranium-235 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined U_238 Uranium-238 levels measured in the sample recorded in picocuries per gram Producer defined ND Not detected above the detection limit Producer defined ScienceBase U.S. Geological Survey-ScienceBase mailing and physical

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Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. 20200817 Jo Ellen Hinck U.S. Geological Survey, Midwest Region Research Fish Biologist mailing and physical

4200 New Haven Road

Columbia MO 65201 USA 573-876-1808 573-876-1863 jhinck@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999