Ackerman, J.T. 20170626 Tabular Digital Data Denver, CO U.S. Geological Survey data release https://doi.org/10.5066/F75H7DG7 Nicole A. Mikoni Robert Poppenga Joshua T. Ackerman Janet Foley Jenny Hazlehurst Güthrum Purdin Linda Aston Sabine Hargrave Karen Jelks Lisa A. Tell 20170512 Publication (Journal) Ecological Indicators 80 Amsterdam, Netherlands ELSEVIER https://doi.org/10.1016/j.ecolind.2017.04.053 Heavy metal contamination (n=17 metals) of feather and tissue samples from live and deceased Anna's hummingbirds (Calypte anna) was evaluated. Feathers taken from live birds are low in sample weight, therefore a novel method was used in feather sample preparation. Feather samples were then analyzed utilizing an inductively coupled plasma mass spectrometry (ICP-MS) assay. In addition, ICP-MS was used to measure heavy metal concentrations in feather and tissue samples from salvaged hummingbird carcasses. Iron, zinc, selenium and mercury concentrations were most commonly elevated in body feathers from live birds with mercury being the metal most likely to be found in elevated concentrations. Because of this, we conducted additional analysis for mercury using atomic absorption spectometry, and this is the mercury data available in this dataset. A strong correlation between total mercury concentrations in feathers and in pectoral muscle was found suggesting that sampling feathers from live hummingbirds may prove a validated, useful, non-lethal sampling method for this species in the wild. These data support the following publication: Nicole A. Mikoni, Robert Poppenga, Joshua T. Ackerman, Janet Foley, Jenny Hazlehurst, Güthrum Purdin, Linda Aston, Sabine Hargrave, Karen Jelks, Lisa A. Tell, Trace element contamination in feather and tissue samples from Anna’s hummingbirds, Ecological Indicators, Volume 80, September 2017, Pages 96-105, https://doi.org/10.1016/j.ecolind.2017.04.053. Heavy metal contamination in birds. Any interested parties in this dataset should contact Josh Ackerman, USGS, before its use 2015 2016 observed Complete Not planned -123.6785888672 -118.6248779297 39.0618491343 37.173448712 Northern California including coastal, valley, and Sierra Nevada foothills. None Anna’s hummingbird Calypte anna Mercury contamination USGS Metadata Identifier USGS:589b6a61e4b0efcedb72d364 None Northern California Sierra Nevada foothills None. Please see 'Distribution Info' for details. The authors of these data require that users direct any questions pertaining to appropriate use or assistance with understanding limitations and interpretation of the data to the individuals/organization listed in the Point of Contact section. Joshua T. Ackerman US Geological Survey, Western Ecological Research Center mailing and physical

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Dixon CA 95620 United States 530-669-5087 jackerman@usgs.gov University of California, Davis and Lindsey Wildlife Museum Environment as of Metadata Creation: Microsoft Windows 7 Version 6.1 (Build 7601) Service Pack 1; Esri ArcGIS 10.3.1 (Build 4959) Service Pack N/A (Build N/A) USGS Biocomplexity Thesaurus Birds Integrated Taxonomic Information System (ITIS) 2017 ONLINE_REFERENCE Washington, D.C. Integrated Taxonomic Information System (ITIS) http://itis.gov comparison and museum materials;;identification keys;;Species, age, and sex of each carcass were determined using guidelines defined in the North American Hummingbird Manual (Scheuhammer 1987). Species, age, and sex of each carcass were determined using guidelines defined in the North American Hummingbird Manual (Scheuhammer 1987). Anna’s hummingbird (Calypte anna) were identified simply as Anna’s hummingbird (Calypte anna). Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Aves Order Apodiformes Family Trochilidae Subfamily Trochilinae Genus Calypte Species Calypte anna Anna's Hummingbird Colibrí cabeza roja U.S. Environmental Protection Agency. 2000. Method 7473, Mercury in solids and solutions by thermal decomposition, amalgamation, and atomic absorption spectrophotometry. In Test Methods for Evaluating Solid Waste, Physical/Chemical Methods; SW 846, Update IVA; U.S. Government Printing Office Washington, D.C. No formal logical accuracy tests were conducted. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. A formal accuracy assessment of the horizontal positional information in the data set has not been conducted. A formal accuracy assessment of the vertical positional information in the data set has either not been conducted, or is not applicable. Development of the data set by the agency / individuals identified in the 'Originator' element in the Identification Info section of the record. Unknown both EPA’s Selected Analytical Methods for Environmental Remediation and Recovery (SAM) lists this method for preparation and/or analysis of solid and wipe samples containing mercury, mercuric chloride (analyzed as total mercury), or methoxyethylmercuric acetate (analyzed as total mercury). U.S. Environmental Protection Agency 1998 Document https://www.epa.gov/homeland-security-research/epa-method-7473-sw-846-mercury-solids-and-solutions-thermal-decomposition Attribute Table Table containing attribute information associated with the data set. Producer defined BirdID Hummingbird identification number Producer defined Hummingbird identification number Age Age of the hummingbird Producer defined AHY After hatch year​ Producer defined HY Hatch year​ Producer defined Sex Whether the hummingbird was a male or female Producer defined F Female Producer defined M Male Producer defined Hg_ppm_dw_Breast_Feather Mercury concentration of breast feathers in parts per million (ppm)​ in dry weight (dw)​ Producer defined 0.276029963 2.201973684 ppm Hg_ppm_dw_Heart_Muscle Mercury concentration of the heart muscle in parts per million (ppm)​​ in dry weight (dw)​​ Producer defined 0.018 0.348950462 ppm Hg_ppm_dw_Head_Feather Mercury concentration of head feathers in parts per million (ppm)​​ in dry weight (dw)​​. NA = No data because feather was missing from the sample Producer defined 0.344897959 3.75254902 ppm Hg_ppm_dw_Pectoral_Muscle Mercury concentration of the pectoral muscle in parts per million (ppm)​​ in dry weight (dw)​​ Producer defined 0.007323371 0.188608049 ppm Hg_ppm_dw_Tail_Feather_R1 Mercury concentration of tail feather​ R1 in parts per million (ppm)​​ in dry weight (dw)​​ Producer defined 0.219484241 1.971787709 ppm The entity and attribute information provided here describes the tabular data associated with the data set. Please review the detailed descriptions that are provided (the individual attribute descriptions) for information on the values that appear as fields/table entries of the data set. The entity and attribute information was generated by the individual and/or agency identified as the originator of the data set. Please review the rest of the metadata record for additional details and information. Joshua T. Ackerman U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Any interested parties in this dataset should contact Josh Ackerman, USGS, before its use. Tabular Digital Data none https://doi.org/10.5066/F75H7DG7 Any interested parties in this dataset should contact Josh Ackerman, USGS, before its use None 20200830 Pai Hui Yu U.S. Geological Survey, PACIFIC REGION Cartographic Technician mailing and physical

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Sacramento CA 95819 US 916-278-9467 916-278-9475 pyu@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999