Erik Hofmeister Melissa Lund Valerie Shearn-Bochsler 20161205 Tabular Digital Data Online U.S. Geological Survey https://dx.doi.org/10.5066/F7707ZM3 Erik Hofmeister Melissa Lund Valerie Shearn-Bochsler Christopher N. Balakrishnan 20161205 Publication (Journal) Online PLOS https://dx.doi.org/10.1371/journal.pone.0167876 The data set contains the results of experimental challenge of captive zebra finches with an American crow isolate of West Nile virus (WNV). Data include infectivity, mortality, viremia, oral shedding of virus, and serology for anti- WNV antibodies. Australian and Timor zebra finches were used in this study and both are useful as a laboratory model of an avian species with moderate susceptibility to WNV. The data were collected to assess the relative susceptibility of captive zebra finches to WNV. 20060501 20141231 ground condition Complete None planned -174.99023437161 -48.427734376667 78.793357529303 7.1440647168087 North america USGS Thesaurus disease None West Nile virus immunoassay qRT-PCR survival analysis USGS Metadata Identifier USGS:5853fa1ce4b0e2663625f350 None National Wildlife Health Center none none Erik Hofmeister U.S. Geological Survey mailing and physical

6006 Schroeder Road

Madison WI 53711 USA 608-270-2476 ehofmeister@usgs.gov Christopher N. Balakrishnan, East Carolina University Greenville, NC Environment as of Metadata Creation: Microsoft Windows 7 Version 6.1 (Build 7601) Service Pack 1; Esri ArcGIS 10.4.1 (Build 5686) Service Pack N/A (Build N/A) USGS Biocomplexity Thesaurus Birds ITIS Unknown ONLINE_REFERENCE Unknown ITIS www.itis.gov Erik Hofmeister U.S. Geological Survey mailing and physical

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Madison WI 53711 USA 608-270-2476 ehofmeister@usgs.gov expert advice avian experts identified bird species Australian and Timor zebra finches were used in this study and both are useful as a laboratory model of an avian species with moderate susceptibility to WNV. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Aves Order Passeriformes Family Estrildidae Genus Taeniopygia Species Taeniopygia guttata Zebra Finch Subspecies Taeniopygia guttata castanotis Subspecies Taeniopygia guttata guttata Summary statistics were computed with R. Linear models and figures were constructed using R. https://www.r-project.org/ download from web Erik Hofmeister U.S. Geological Survey mailing and physical

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Madison WI 53711 USA 608-270-2476 ehofmeister@usgs.gov R Core Team 2013 Software Summary statistics were computed with GraphPad Prism http://www.graphpad.com/scientific-software/prism/ Purchase from web Erik Hofmeister U.S. Geological Survey mailing and physical

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Madison WI 53711 USA 608-270-2476 ehofmeister@usgs.gov GraphPad Software 2016 Software All data recorded in the laboratory were spot checked by the lead researcher (EH) before being transferred and recorded into digital files No formal logical accuracy tests were conducted Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted No formal positional accuracy tests were conducted Virus was cultured on Vero cells according to standard methods (Beaty, 1989). 20150930 Viral RNA was amplified using standard methods (Lanciotti, 2000) 20150930 Sera were tested for anti-WNV antibodies using the WNV wild bird IgG enzyme immunoassay ( EIA) (Ebel et al. 2002) with slight modifications as previously described (Hofmeister et al. 2016). Sera were also tested for neutralizing antibodies to WNV with the plaque reduction neutralization test (PRNT) (Beaty, 1989). 20150930 Lab [4:12 PM] Burgess, Jodi L Hofmeister, E.K., Newhouse, D.J., and Balakrishnan, C.N., 2017, Transcriptional response to West Nile virus infection in the zebra finch (Taeniopygia guttata), a songbird model for immune function: U.S. Geological Survey data release, https://dx.doi.org/10.5066/F7G44NHF ​[4:17 PM] Burgess, Jodi L https://www.sciencebase.gov/catalog/item/5a0b1033e4b09af898cb6e7a ​[4:17 PM] Burgess, Jodi L ​[4:20 PM] Burgess, Jodi L Verant, M.L., Bohuski, E.A., Richgels, K.L.D., Olival, K.J., and Blehert, D.S., 2017, Determinants of Pseudogymnoascus destructans within bat hibernacula: data: U.S. Geological Survey data release, https://doi.org/10.5066/F77D2SP5. ​[4:25 PM] Burgess, Jodi L https://www.sciencebase.gov/catalog/item/5b619edbe4b006a11f6b6bfa ​[4:25 PM] Burgess, Jodi L IP-093244 ​[4:26 PM] Burgess, Jodi L Bron, G.M., Rocke, T.E., Richgels, K.L.D., Samuel, M.D., Matteson, J.P., Poje, J.E., Osorio, J.E., Lorenzsonn, F., and Boulerice, J., 2018, Impact of the oral sylvatic plague vaccine on non-target small rodents: Data: U.S. Geological Survey data release, https://doi.org/10.5066/P9WCSCDK ​[4:34 PM] Burgess, Jodi L https://www.sciencebase.gov/catalog/item/5a1ef1b6e4b09fc93dd9743b ​[4:35 PM] Burgess, Jodi L Abbott, R.C., Richgels, K.L.D., Russell, R.E., and Rocke, T.E., 2017, SPV field trials bait uptake data: U.S. Geological Survey data release, https://doi.org/10.5066/F7X92968 ​[4:39 PM] Wesenberg, Katherine E Experimental three groups of West Nile virus challenge groups of domesticated zebra finches were inoculated with 100 microliters of BA1 containing 107,105, and 103 plaque forming units (PFU) of a low passage (< 3) 1999 American crow (Corvus brachyrhynchos) isolate of WNV (NWHC 16399-3). Each of these inoculated groups was randomly divided into two sub-groups with the sub-groups sampled on an alternate day schedule on 1 – 8 days post-inoculation (dpi). All surviving birds were sampled on 10 and 14 dpi. Blood was obtained from the jugular vein, allowed to clot, and the viral load in the serum was tested by plaque assay in vero cells.Plotted are plaque forming units of virus present in the serum at various time points following challenge. Beaty B, Calisher C, Shope R. Arboviruses. In: Schmidt N, Emmons R, editors 1989 Document None Ebel GD, Dupuis II AP, Nicholas D,Young D, Maffei J, Kramer LD 2002 Document None Hofmeister, E, Jankowski M, Goldberg D, Franson J 2016 Document None Lanciotti RS, Kerst AJ, Nasci RS, Godsey MS, Mitchell CJ, Savage HM, et al. 2000 Document None Mean Peak Virenia Attribute Table Table containing attribute information associated with the data set. Producer defined Challenge quantity of plaque forming units of WNV challenge Producer defined quantity of plaque forming units of WNV challenge DPI_0 day inoculated with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined 0 0 DPI-1 1 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 0 6.867 DPI-2 2 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 1.38 6.789 DPI-3 3 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 1.38 10.293 DPI-4 4 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 1.38 6.57 DPI-5 5 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 1.38 6.31 DPI-6 6 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 1.38 6.19 DPI-7 7 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 0 5.22 DPI-8 8 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 0 5.95 DPI-10 10 day post inoculation with WNV, quantity is log10 plaque forming units of WNV cultured Producer defined NULL Not sampled Producer defined 0 3 Days_of_peak_viremia_10^7 day post inoculation with 10^7 WNV peak viremia was recorded Producer defined NULL Not sampled Producer defined 1 3 days Days_of_peak_viremia_10^5 day post inoculation with 10^5 WNV peak viremia was recorded Producer defined NULL Not sampled Producer defined 1 7 days Days_of_peak_viremia_10^3 day post inoculation with 10^3 WNV peak viremia was recorded Producer defined NULL Not sampled Producer defined 4 6 days PFUs Attribute Table Table containing attribute information associated with the data set. Producer defined BID Bird ID Producer defined Bird ID DAY day post inoculation with WNV Producer defined 0 8 day log10PFU log10 plaque forming units of WNV cultured Producer defined 0 10 SPECIES Species of bird Producer defined ZFAU Australian zebra finch Producer defined DOSE log10 plaque forming units of WNV challenge Producer defined 3 7 S Table Attribute Table Table containing attribute information associated with the data set. Producer defined Run RT-PCR run to detect presence of WNV in serum Producer defined 7 11 run AU Arbitrary units of WNV RNA copies detected Producer defined 2 7 Ct cross-over threshold determined by ABI real time instrument Producer defined 15.29 33.56 Dup_ single RT-PCR set-up Producer defined 1 1 Study 1 Schema Attribute Table Table containing attribute information associated with the data set. Producer defined BID Bird ID Producer defined Bird ID Challenge quantity of plaque forming units of WNV challenge (or control) Producer defined quantity of plaque forming units of WNV challenge (or control) DPI--7 day pre inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 1 serum collected Producer defined DPI-0 day of inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 0 no sample collected Producer defined DPI-1 1 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined DPI-2 2 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined DPI-3 3 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined DPI-4 4 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined DPI-5 5 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined DPI-6 6 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined DPI-7 7 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined M Mortality Producer defined 2 serum and oral swab collected Producer defined DPI-8 8 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined M Mortality Producer defined 2 serum and oral swab collected Producer defined DPI-10 10 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not Sampled Producer defined 2 serum and oral swab collected Producer defined 2,6 serum and oral swab collected and serum, oral swab, skin, lung, heart, liver, spleen, kidney, brain collected Producer defined M Mortality Producer defined DPI-14 14 day post inoculation with WNV Producer defined 2,6 serum and oral swab collected and serum, oral swab, skin, lung, heart, liver, spleen, kidney, brain collected Producer defined Serum_Virus_Culture result of serum viral culture Producer defined N none detected Producer defined P virus detected Producer defined EIA_Result result of IgG EIA Producer defined N none detected Producer defined NT Not tested Producer defined P virus detected Producer defined PRNT_90 result of PRNT assay with a 90% inhibition scoring rule Producer defined N none detected Producer defined NT Not tested Producer defined P virus detected Producer defined PRNT_80 result of PRNT assay with a 80% inhibition scoring rule Producer defined N none detected Producer defined NT Not tested Producer defined P virus detected Producer defined Swab_Virus_Culture result of oral swab viral culture Producer defined N none detected Producer defined P virus detected Producer defined log10_Peak_PFU_Swab PFU/Swab: quantity of WNV cultured per swab Producer defined N none detected Producer defined 1.69 3.84 Study 1 Survival Attribute Table Table containing attribute information associated with the data set. Producer defined Death_Time day post inoculation with WNV that death was recorded Producer defined 7 14 day WNV_Challenge_Level_10^7 10^7 WNV challenge Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 no sample collected Producer defined 1 serum collected Producer defined WNV_Challenge_Level_10^5 10^5 WNV challenge Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 no sample collected Producer defined 1 serum collected Producer defined WNV_Challenge_Level_10^3 10^3 WNV challenge Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 no sample collected Producer defined Study 2 Schema Attribute Table Table containing attribute information associated with the data set. Producer defined BID Bird ID Producer defined Bird ID Challenge quantity of plaque forming units of WNV challenge (or control) Producer defined quantity of plaque forming units of WNV challenge (or control) DPI-2 2 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined 6 serum, oral swab, skin, lung, heart, liver, spleen, kidney, brain collected Producer defined EIA_Result-2 2 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined ND not done Producer defined Swab_Virus_Culture-2 2 day post inoculation result of oral swab viral culture Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined Tissues_RT_PCR-2 2 day post inoculation result of RT-PCR of kidney or liver Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined P virus detected Producer defined Serum_RT_PCR-2 2 day post inoculation result of RT-PCR of serum Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined ND not done Producer defined P virus detected Producer defined DPI-3 3 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined EIA_Result-3 3 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined DPI-4 4 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined 6 serum, oral swab, skin, lung, heart, liver, spleen, kidney, brain collected Producer defined EIA_Result-4 4 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined P virus detected Producer defined Swab_Virus_Culture-4 4 day post inoculation result of oral swab viral culture Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined P virus detected Producer defined DPI-5 5 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined EIA_Result-5 5 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined P virus detected Producer defined DPI-7 7 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined 6 serum, oral swab, skin, lung, heart, liver, spleen, kidney, brain collected Producer defined EIA_Result-7 7 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined P virus detected Producer defined DPI-10 10 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined EIA_Result-10 10 day post inoculation result of IgG EIA Producer defined ND not done Producer defined DPI-14 14 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined EIA_Result-14 14 day post inoculation result of IgG EIA Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined PRNT_Result-14 14 day post inoculation result of PRNT assay with a 90% inhibition scoring rule Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined Serum_Virus_Culture-14 14 day post inoculation result of serum virus culture on any day of study Producer defined N none detected Producer defined P virus detected Producer defined Swab_Virus_Culture-14 14 day post inoculation result of swab virus culture on any day of study Producer defined N none detected Producer defined Tissues_Virus_Culture-14 14 day post inoculation result of tissue virus culture on any day of study Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined Tissues_RT_PCR-14 14 day post inoculation result of tissue RT-PCR on any day of study Producer defined N none detected Producer defined ND not done Producer defined Study 3 AUs by dpi Attribute Table Table containing attribute information associated with the data set. Producer defined AZF2 Australian zebra finch days 2, quantity recorded is arbitrary units of WNV RNA detected Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0.02 2.24 AZF3 Australian zebra finch days 3, quantity recorded is arbitrary units of WNV RNA detected Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0.7 3.93 AZF4 Australian zebra finch days 4, quantity recorded is arbitrary units of WNV RNA detected Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0.57 4.75 AZF5 Australian zebra finch days 5, quantity recorded is arbitrary units of WNV RNA detected Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 3.9 AZF10 Australian zebra finch days 10, quantity recorded is arbitrary units of WNV RNA detected Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 0.54 TZFH2 Timor zebra finch challenged with 105 WNV days 2, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0.15 2.85 TZFH3 Timor zebra finch challenged with 105 WNV days 3, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 3.32 TZFH4 Timor zebra finch challenged with 105 WNV days 4, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2.58 6.6 TZFH5 Timor zebra finch challenged with 105 WNV days 5, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 3.39 5.82 TAFH10 Timor zebra finch challenged with 105 WNV days 10, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 1.3 TZFL2 Timor zebra finch challenged with 103 WNV days 2, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 2.9 TZFL3 Timor zebra finch challenged with 103 WNV days 3, quantity recorded is arbitrary units of WNV RNA detected. Producer defined 2.08 5 TZFL4 Timor zebra finch challenged with 103 WNV days 4, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 1.63 4.44 TZFL5 Timor zebra finch challenged with 103 WNV days 5, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 3.84 7.04 TZFL10 Timor zebra finch challenged with 103 WNV days 10, quantity recorded is arbitrary units of WNV RNA detected. Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0 0.96 Study 3 EIA AU Attribute Table Table containing attribute information associated with the data set. Producer defined EIA_AU_AZF_10_dpi Result of IgG EIA serology in arbitrary units Australian zebra finch at 10 dpi Producer defined 0.05 5.21 EIA_AU_TZF__10_dpi Result of IgG EIA serology in arbitrary units Timor zebra finch at 10 dpi Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 1.43 8.13 EIA_AU_AZF_21_dpi Result of IgG EIA serology in arbitrary units Australian zebra finch at 21 dpi Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 0.88 5.08 EIA_AU_TZF__21_dpi Result of IgG EIA serology in arbitrary units Timor zebra finch at 10 dpi Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2.42 4.12 Study 3 Fig 2 AUs Attribute Table Table containing attribute information associated with the data set. Producer defined BID Bird ID Producer defined Bird ID DAY day post inoculation with WNV Producer defined 0 10 day Aus Arbitrary units of WNV RNA copies detected Producer defined 0 7 Species Bird species Producer defined AZF Australian zebra finch Producer defined TZF Timor zebra finch Producer defined logDOSE log10 quantity of plaque forming units of WNV challenge Producer defined 3 5 Study 3 Schema Attribute Table Table containing attribute information associated with the data set. Producer defined Species Bird Species Producer defined A Australian zebra finch Producer defined T Timor zebra finch Producer defined BID Bird ID Producer defined Bird ID WNV_Challenge quantity of plaque forming units of WNV challenge Producer defined quantity of plaque forming units of WNV challenge DPI-1 1 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 1 serum collected Producer defined Mass-1 1 day post inoculation weight of bird Producer defined ND not done Producer defined 9 14.92 g DPI-2 2 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined ND not done Producer defined Mass-2 2 day post inoculation weight of bird Producer defined ND not done Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 8.85 14.58 g RT_PCR-2 2 day post inoculation result of detection of WNV by PCR in serum Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined EIA_Results-2 2 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined P virus detected Producer defined N none detected Producer defined ND not done Producer defined DPI-3 3 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined ND not done Producer defined Mass-3 3 day post inoculation weight of bird Producer defined 8.87 14.18 g RT_PCR-3 3 day post inoculation result of detection of WNV by PCR in serum Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined ND not done Producer defined P virus detected Producer defined EIA_Results-3 3 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined ND not done Producer defined DPI-4 4 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined ND not done Producer defined Mass-4 4 day post inoculation weight of bird Producer defined ND Not done Producer defined 8.91 14.29 g RT_PCR-4 4 day post inoculation result of detection of WNV by PCR in serum Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined EIA_Results-4 4 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined ND not done Producer defined DPI-5 5 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 serum and oral swab collected Producer defined ND not done Producer defined Mass-5 5 day post inoculation weight of bird Producer defined 8.82 14.85 g RT_PCR-5 5 day post inoculation result of detection of WNV by PCR in serum Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined EIA_Results-5 5 day post inoculation result of IgG EIA Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined DPI-10 10 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 1 serum collected, 2= serum and oral swab collected Producer defined M Mortality Producer defined Mass-10 10 day post inoculation weight of bird Producer defined ND Not done Producer defined 8.61 14.2 g RT_PCR-10 10 day post inoculation result of detection of WNV by PCR in serum Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined EIA_Results-10 10 day post inoculation result of IgG EIA Producer defined BL Baseline Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined DPI-14 14 day post inoculation with WNV Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined M Mortality Producer defined DPI-21 21 day post inoculation with WNV Producer defined 1 serum collected Producer defined ND not done Producer defined Mass-21 21 day post inoculation weight of bird Producer defined ND Not done Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 9.28 14.45 g EIA_Results-21 21 day post inoculation result of IgG EIA Producer defined BL Baseline Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined Infection_Status Infection status Producer defined N no WNV detected Producer defined P RT-PCR or serology detection of WNV Producer defined EIA_Results result of IgG EIA Producer defined BL Baseline Producer defined N none detected Producer defined ND not done Producer defined P virus detected Producer defined PRNT90_Titer result of PRNT assay with a 90% inhibition scoring rule Producer defined N Not detected Producer defined ND Not done Producer defined 20 2560 Peak_day_viremia DPI with peak detection of WNV by RT-PCR Producer defined ND Not done Producer defined {Null Value / Empty Field Entry} Not sampled Producer defined 2 5 days Swab_virus_culture result of oral swab viral culture Producer defined N none detected Producer defined P virus detected Producer defined Study 3 wtdeltas Attribute Table Table containing attribute information associated with the data set. Producer defined BID Bird ID Producer defined Bird ID SP Species and level of WNV challenge Producer defined Aus 10^5 Aus 10^5= Australian zebra finch 105 WNV challenge Producer defined Tim 10^3 Timor zebra finch 105 WNV challenge Producer defined Tim 10^5 Timor zebra finch 103 WNV challenge Producer defined WtDelta Difference between previous weight and that particular day Producer defined -2 1 Daypi Days post inoculation Producer defined 0 10 DxMethod Diagnostic method in this case virus culture or serological evidence of infection Producer defined 1 virus culture Producer defined 2 serological evidence of infection Producer defined NA Not available Producer defined U.S. Geological Survey - ScienceBase mailing and physical

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Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. 20250805 Erik K Hofmeister U.S. Geological Survey, Midwest Region Veterinary Medical Officer mailing and physical

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Madison WI 53711 USA 608-270-2476 608-270-2418 ehofmeister@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999