Baldigo, B.P. George, S.D. 20160915 spreadsheet Reston, VA U.S. Geological Survey data release https://dx.doi.org/10.5066/F78913ZC Community composition data from multi-pass electrofishing surveys for assessing fish populations in headwater streams of the Adirondack Mountains, New York, USA. Each row represents one individual fish. This spreadsheet contains 11 columns. The first 7 columns describe the data (year collected, local site ID, stream name, latitude, longitude, USGS site number, and electrofishing pass number) and the remaining 4 columns describe each individual fish (common name, scientific name, total length (mm) and weight (g)). These data support the following publication: Baldigo, B.P., Sporn, L.A., George, S.D., and Ball, Jacob, in press. Efficacy of environmental DNA to detect and quantify Brook Trout Salvelinus fontinalis, populations in streams of the Adirondack Mountains, New York. Trans. Amer. Fish. Soc. na, na; http://dx.doi.org/10.1577/. Data were collected to assess fish communities and estimate population size and biomass for each species at each sampling site. 2014 2015 observed Complete Not planned -75.583190918549 -73.726501465498 44.307813589314 43.128732978164 All data collected from western portion of Adirondack Park or immediately adjacent to it. None environmental DNA eDNA population metrics survey methods Adirondack streams brook trout Salvelinus fontinalis USGS Biocomplexity Thesaurus DNA identification population methods USGS Metadata Identifier USGS:57dc3b20e4b090824ffd5673 U.S. Department of Commerce, 1995, Countries, dependencies, areas of special sovereignty, and their principal administrative divisions, Federal Information Processing Standard 10-4,): Washington, D.C., National Institute of Standards and Technology United States U.S. US U.S. Department of Commerce, 1987, Codes for the identification of the States, the District of Columbia and the outlying areas of the United States, and associated areas (Federal Information Processing Standard 5-2): Washington, D.C., National Institute of Standards and Technology NY Counties and County Equivalents of the States of the United States and the District of Columbia (FIPS Pub 6-3) St. Lawrence County Herkimer County Hamilton County Essex County Lewis County Oneida County Geographic Names Information System New York Adirondack State Park none none Scott George U.S. Geological Survey mailing and physical

425 Jordan Road

Troy New York 12180 Rensselaer County 518-285-5639 sgeorge@usgs.gov New York State Energy Research and Development Authority, New York State Department of Environmental Conservation USGS Biocomplexity Thesaurus Fishes Barry, Baldigo Scott, George Walt, Keller 2015 spreadsheet Steuben, Maine Northeastern Naturalist, v.22, no. 2, p. 345-371 http://dx.doi.org/10.1656/045.022.0209 Scott George U.S. Geological Survey mailing and physical

425 Jordan Road

Troy New York 12180 USA 518-285-5639 sgeorge@usgs.gov identification keys All fishes were identified to species. Freshwater fishes captured during electrofishing surveys were identified to species. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Semotilus Species Semotilus atromaculatus creek chub Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Pimephales Species Pimephales promelas fathead minnow Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Notemigonus Species Notemigonus crysoleucas golden shiner Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Chrosomus Species Chrosomus eos northern redbelly dace Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Scorpaeniformes Suborder Cottoidei Superfamily Cottoidea Family Cottidae Genus Cottus Species Cottus cognatus slimy sculpin Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Perciformes Suborder Percoidei Family Percidae Genus Etheostoma Species Etheostoma olmstedi tessellated darter Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Exoglossum Species Exoglossum maxillingua cutlips minnow cutlip minnow Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Perciformes Suborder Percoidei Family Centrarchidae Genus Lepomis Species Lepomis gibbosus Pumpkinseed Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Luxilus Species Luxilus cornutus common shiner Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Margariscus Species Margariscus nachtriebi northern pearl dace Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Perciformes Suborder Percoidei Family Centrarchidae Genus Micropterus Species Micropterus dolomieu smallmouth bass Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Perciformes Suborder Percoidei Family Percidae Genus Perca Species Perca flavescens yellow perch Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Rhinichthys Species Rhinichthys atratulus blacknose dace eastern blacknose dace Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Rhinichthys Species Rhinichthys cataractae longnose dace Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Protacanthopterygii Order Esociformes Family Umbridae Genus Umbra Species Umbra limi central mudminnow Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Protacanthopterygii Order Salmoniformes Family Salmonidae Subfamily Salmoninae Genus Salvelinus Species Salvelinus fontinalis brook trout No formal attribute accuracy tests were conducted No formal logical accuracy tests were conducted Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted No formal positional accuracy tests were conducted Data were transcribed from field forms. 20151001 n/a Point 1.0E-5 1.0E-5 Degrees, minutes, and decimal seconds NAD 83 USGS_eDNA_fishdatafile Community composition data for assessing fish populations in headwater streams of the Adirondack Mountains, New York, USA. This spreadsheet contains 11 columns. The first 7 columns describe the data (year collected, local site ID, stream name, latitude, longitude, USGS site number, and electrofishing pass number) and the remaining 4 columns describe each individual fish (common name, scientific name, total length (mm) and weight (g)). Scott George Year Year in which fish community survey was conducted. Calendar 2014 2015 Site ID Site Identification Code Producer defined Four number identifier. Stream Name Name of stream/river from which fish were collected. Producer defined Name of stream/river from which fish were collected. Latitude (degrees minutes seconds) Latitude provided in decimal degrees. Producer defined -90 90 Degrees Minutes Seconds and Tenths of Seconds Longitude (degrees minutes seconds) Longitude provided in decimal degrees. Producer defined -180 180 Degrees Minutes Seconds and Tenths of Seconds USGS Station Number Site Identification Code Producer defined 8-15 digit identifier Pass number Electrofishing pass number. Producer defined 1 4 Common Name Common name of fish species. Producer defined Blank indicates no fish captured in the pass. Scientific name Scientific name of fish species. Producer defined Blank indicates no fish captured in the pass. Fish Length (mm) Total fish length in millimeters. Producer defined To the nearest millimeter. Fish weight (g) Fish weight in grams. Producer defined To the nearest tenth gram. Sciencebase Team U.S. Geological Survey - ScienceBase mailing and physical

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov The data are released on the condition that neither the USGS nor the U.S. Government shall be held liable for any damages resulting from its authorized or unauthorized use. Although these data have been processed successfully on a computer system at the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. The USGS or the U.S. Government shall not be held liable for improper or incorrect use of the data described and/or contained herein. 20200825 Scott George U.S. Geological Survey Biologist mailing and physical

425 Jordan Road

Troy NY 12180 USA 518-285-5639 sgeorge@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999