Marissa Wulff Larry Brown Jason May 2017 CSV Reston, VA U.S. Geological Survey http://dx.doi.org/10.5066/F72B8W48 Data included in this dataset: Fish count data for the Santa Ana Sucker (Catostomus santaanae) and the Arroyo Chub (Gila orcutti) in the upper Santa Ana River for 2015. Collection of additional data on the Santa Ana Sucker (Catostomus santaanae) and the Arroyo Chub (Gila orcutti) has been identified as a needed task to support development of the upper Santa Ana River Habitat Conservation Plan (HCP; http://www.uppersarhcp.com/). The ability to monitor population abundance and understanding the habitats used by species are important when developing such plans. The Santa Ana Sucker (Catostomus santaanae) is listed as a threatened species under federal legislation and is considered a species of special concern in California by the California Department of Fish and Wildlife (Moyle 2002). The Arroyo Chub (Gila orcutti) is considered a species of special concern in California by the California Department of Fish and Wildlife (Moyle 2002). Both species are present in the Santa Ana River watershed in the area being evaluated for establishment of the upper Santa Ana River Habitat Conservation Plan (HCP; http://www.uppersarhcp.com/, Fig. 1). The HCP is a collaborative effort involving the water resource agencies of the Santa Ana River Watershed, the US Fish and Wildlife Service, California Department of Fish and Wildlife, and other government agencies and stakeholder organizations. The goals of the HCP are to: 1) enable the water resource agencies to provide a reliable water supply for human uses; 2) conserve and maintain natural rivers and streams that provide habitat for a diversity of unique and rare species; and 3) maintain recreational opportunities for activities such as hiking, fishing, and wildlife viewing, provided by the protection of these habitats and the river systems they depend on. The HCP will specify how species and their habitats will be protected and managed in the future and will provide the incidental take permits needed by the water resource agencies under the federal and State endangered species acts to maintain, operate, and improve their water resource infrastructure. Although the Santa Ana Sucker has been the subject of various research and monitoring studies within its remaining habitat in California (see below for more detail), initial assessment of the available data within the HCP suggested that additional data on population size, fish habitat use and availability of suitable habitat would be needed to support development of the HCP. Similarly, work on the Arroyo Chub has been limited and there is little data on the species within the HCP area, particularly the mainstem Santa Ana River. Thus, the collection of additional data on these two species has been identified as a needed task to support development of the HCP. The goals of the current study are: 1. Compare snorkeling, seining, and electrofishing as methods for estimating native fish abundance. 2. Develop a population estimate for native fish species in the study area based on the results from Goal 1. 3. Develop a habitat suitability model for the Santa Ana River for Santa Ana Sucker, and if possible Arroyo Chub. 20150701 present ground condition In work Annually -117.45208740155 -117.11700439375 34.211440334249 33.942996974552 Upper Santa Ana River, CA, USA USGS Thesaurus Santa Ana Sucker (Catostomus santaanae) population estimate habitat use Arroyo Chub (Gila orcutti) USGS Metadata Identifier USGS:5797ef00e4b0589fa1c61c0a None Santa Ana River California none none Marissa Wulff USGS mailing and physical

6000 J Street

Sacramento CA 95819 USA 9162783000 mwulff@usgs.gov Water resource agencies of the Santa Ana River Watershed, the US Fish and Wildlife Service, California Department of Fish and Wildlife USGS Biocomplexity Thesaurus Amphibians Crustaceans Fishes Peter Moyle 2002 BOOK Berkeley University of California Press Unknown Larry Brown USGS mailing and physical

6000 J Street

Sacramento CA 95819 USA 9162782000 lrbrown@usgs.gov expert advice Fish were positively identified to species by experts. All organisms were identified to the level of species. Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cobitoidea Family Catostomidae Subfamily Catostominae Tribe Catostomini Genus Catostomus Species Catostomus santaanae Santa Ana sucker Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Cypriniformes Superfamily Cyprinoidea Family Cyprinidae Genus Gila Species Gila orcuttii Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Ostariophysi Order Siluriformes Family Ictaluridae Genus Ameiurus Species Ameiurus natalis yellow bullhead bagre torito amarillo barbotte jaune Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Cyprinodontiformes Suborder Cyprinodontoidei Family Poeciliidae Subfamily Poeciliinae Genus Gambusia Species Gambusia affinis mosquitofish western mosquitofish guayacón mosquito Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Perciformes Suborder Percoidei Family Centrarchidae Genus Micropterus Species Micropterus salmoides largemouth bass lobina negra achigan à grande bouche Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Reptilia Order Testudines Suborder Cryptodira Superfamily Testudinoidea Family Emydidae Subfamily Deirochelyinae Genus Trachemys Species Trachemys scripta Subspecies Trachemys scripta elegans Red-eared Slider Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Osteichthyes Class Actinopterygii Subclass Neopterygii Infraclass Teleostei Superorder Acanthopterygii Order Perciformes Suborder Percoidei Family Centrarchidae Genus Lepomis Species Lepomis cyanellus pez sol Green Sunfish crapet vert Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Amphibia Order Anura Family Ranidae Genus Lithobates Species Lithobates catesbeianus American Bullfrog Kingdom Animalia Subkingdom Bilateria Infrakingdom Protostomia Superphylum Ecdysozoa Phylum Arthropoda Subphylum Crustacea Class Malacostraca Subclass Eumalacostraca Superorder Eucarida Order Decapoda Suborder Pleocyemata Infraorder Astacidea Superfamily Astacoidea Family Cambaridae Subfamily Cambarinae Genus Procambarus Subgenus Procambarus (Scapulicambarus) Species Procambarus clarkii red swamp crayfish red swamp crawfish Kingdom Animalia Subkingdom Bilateria Infrakingdom Deuterostomia Phylum Chordata Subphylum Vertebrata Infraphylum Gnathostomata Superclass Tetrapoda Class Amphibia Order Anura Family Pipidae Genus Xenopus Species Xenopus laevis African Clawed Frog Rana-de garras africana No formal attribute accuracy tests were conducted Data was checked for values falling within the expected ranges. Data was checked for data duplication/omission. Data set is considered complete for the information presented, as described in the abstract. Users are advised to read the rest of the metadata record carefully for additional details. No formal positional accuracy tests were conducted No formal positional accuracy tests were conducted Population estimates based on the electrofishing data were calculated using standard maximum likelihood techniques. The MicroFish software package was utilized for calculations (http://www.microfish.org/). Population estimates and 95% confidence intervals were calculated for each reach where electrofishing was completed and depletion accomplished. Population estimates were compared among methods. Total numbers of fish in a reach were calculated as the mean of the two snorkeling surveys and as the sum of the number of fish caught by seining plus the number of fish estimated from electrofishing. Each of the estimates were then used to estimate populations within sections by multiplying the number of fish per kilometer of stream by the estimated stream length within each stream section. 20160701 Field Fish sampling using all three methods described below was conducted from 8-21 September, 2015, excluding weekends. Sampling was interrupted by a storm on 15 September that resulted in flows of over 5,000 cubic feet per second at the E Street gage (USGS station 11059300). Sampling was suspended until 17 September, when flows had receded to manageable levels; however; turbidity remained high and snorkeling was not possible for the reaches in segment 5, below Riverside Drive. The unexpected storm provided an opportunity to assess the effects of high flows on fishes, so we conducted single pass snorkeling counts without block nets for the reaches above Riverside Drive (reaches 1-12) on 23 September and 3 November 2015; otherwise, each reach was isolated using block nets consisting of 6 mm mesh seine material. Each reach was sampled with three methods: snorkeling, seining, and backpack electrofishing, in that order. These methods are all widely used in studies of stream fish (Murphy and Willis, 1996; Bonar and others, 2009). Each sampling reach was snorkeled twice by one or two swimmers who counted fishes of all species while moving upstream. Numbers and estimated total lengths to the nearest 10 mm were recorded during each pass by the snorkeler(s) on PVC slates. The second snorkeling pass was conducted after the snorkeler(s) returned to the bottom of the reach and transferred data from the slates to paper notes, usually about 30 minutes. After snorkeling was completed the reach was seined. The reach was seined twice using the following technique. Seines were made of 6 mm mesh seine material. The seine was set and then “sweepers” positioned upstream would chase fish downstream into the seine by rapidly walking toward the seine will sweeping a dip net back and forth through the water column. The seine was then lifted and captured fish transferred to a bucket. The sweepers would then return to their positions and the process repeated a second time. The whole crew would then move upstream and set the seine just above where the sweepers started on the previous pass. In narrow reaches the seine was set across the entire stream. In wider reaches, the seining crew zig zagged from bank to bank. After completion of the first pass, the seining team immediately returned to the bottom of the reach and started the second pass. All fish captured were maintained in live wells outside of the sampling reach until processed. The passes were not kept separate for processing. After seining was complete, the reach as electrofished, using one or two electrofishers. A minimum of three passes was made through the sampling reach (Riley and Fausch 1992). Shocked fish were captured using 6 mm mesh dip nets and immediately placed in buckets of native stream water. Fish were moved to live wells as soon as possible. The fish from each pass of seining and electrofishing were counted, measured, and then returned to the stream. Within each segment, a minimum subsample of 30 fish of each fish species captured were weighed. Sampling was conducted in cooperation with the U.S. Fish and Wildlife Service and Riverside-Corona Resource Conservation District personnel who are permitted to take protected species, including electrofishing. All conditions of those permits pertaining to fish handling were followed. Electrofisher settings were 300 volts, 30 Hertz, and 25% duty cycle. Past experience of the permit holders indicated these settings captured suckers with minimal or no mortality. Bonar, S.A., Hubert, W.A., and Willis, D.W., editors, 2009, Standard methods for sampling North American freshwater fishes: American Fisheries Society, Bethesda, Maryland. Murphy, B.R., and Willis, D.W., editors, 1996, Fisheries techniques, 2nd edition: American Fisheries Society, Bethesda, Maryland. Riley, S.C., and K.D. Fausch, 1992, Underestimation of trout population size by maximum-likelihood removal estimates in small streams: North American Journal of Fisheries Management, v. 12, p.768–776. Garmin GPS unit in NAD83 Point 1.0E-5 1.0E-5 Decimal degrees Santa Ana River fish population estimate data This data set contains all fish collected during population estimate collections Field personnel Section Site description information Producer defined Site location description based on closest major landmarks Segment Segments are user defined larger sections of river composed of multiple reaches. They are numbered from upstream to downstream. Producer defined 1 6 Reach Reaches are user defined sections of the river where sampling activities occurred. They are numbered from upstream to downstream. Producer defined 1 6 Sampled after storm A record for whether the site was sampled after a major storm on 15 September 2015 Producer defined Yes Sampling took place after the storm on 15 September 2015 Producer defined No Sampling took place before the storm on 15 September 2015 Producer defined Date Date of collection MM/DD/YYY Date Latitude Latitude of site location in decimal degrees NAD 83 DDD.DDDDD° Longitude Longitude of site location in decimal degrees NAD 83 DDD.DDDDD° Method Survey method Producer defined Sampling method for survey Pass Sampling pass number Producer defined Sampling pass Observer Name of field personnel observing fish Producer defined The name of individual observing fish Common name Common name of organisim Producer defined Common name of organisim Count Number of organisms observed during snorkeling or captured by seining or electrofishing Producer defined observed during snorkeling or captured by seining or electrofishing Total length (mm) Total length of the fish is measured from the tip of the snout to the tip of the longer lobe of the caudal fin. Standard fish measurement 0 1000000 millimeters Fork length (mm) Fork length is measured from the tip of the snout to the end of the middle caudal fin rays Standard fish measurement 0 100000 millimeters Weight (g) Weight of fish in grams (g) Producer defined 0 1000000 Grams Note Any additional details that may be important to the sampling effort or data collection. Producer defined Observational comments Start time Start time of electrofishing pass in 24-hour time Military time 0100 2400 Hours:minutes End time End time of electrofishing pass in 24-hour time Military time 0100 2400 Hours:minutes Shocker1seconds Electrofishing time duration in seconds of unit 1 Producer defined; recorded on electrofishing unit An automated feature of the electrofishing unit Shocker2seconds Electrofishing time duration in seconds of unit 2 Producer defined; recorded on electrofishing unit An automated feature of the electrofishing unit NA Abbreviation of not applicable Producer defined Not applicable (NA) is used to indicate a data value was not recorded because it does not apply. NV Abbreviation of no value Producer defined No value (NV) is used to indicate that data was not collected. U.S. Geological Survey - ScienceBase U.S. Geological Survey - ScienceBase mailing and physical

Denver Federal Center, Building 810, Mail Stop 302

Denver CO 80225 USA 1-888-275-8747 sciencebase@usgs.gov Unless otherwise stated, all data, metadata and related materials are considered to satisfy the quality standards relative to the purpose for which the data were collected. Although these data and associated metadata have been reviewed for accuracy and completeness and approved for release by the U.S. Geological Survey (USGS), no warranty expressed or implied is made regarding the display or utility of the data on any other system or for general or scientific purposes, nor shall the act of distribution constitute any such warranty. 20200812 Marissa Wulff USGS mailing and physical

6000 J Street

Sacramento CA 95819 USA 9162783000 mwulff@usgs.gov FGDC Biological Data Profile of the Content Standard for Digital Geospatial Metadata FGDC-STD-001.1-1999