This U.S. Geological Survey (USGS) data release consists of data from two tracer investigations in the Snake Creek drainage of Great Basin National Park, White Pine County, Nevada. The first investigation, occurring from April to September 2019, involved injecting a fluorescein dye tracer below a diversion point in Snake Creek and monitoring 13 sites in the Snake Creek and neighboring Big Wash drainages for presence of the dye. The results of the first investigation were used to select a targeted monitoring location for a second, quantitative investigation. The second investigation, occurring from September 2020 to March 2021, involved injecting two tracers, sodium bromide and fluorescein dye, below the same diversion point and monitoring the quantity of tracer in discharge measured at Spring Creek Spring (USGS site 385433114063901). The data release includes three tables in Tab Separated Values (TSV) format: 1) Fluorescein_2019Reconnaissance.tsv - Dye recovery classifications at 13 sites during a reconnaissance dye tracer investigation in the Snake Creek and Big Wash drainages of Great Basin National Park, White Pine County, Nevada, April to September 2019. 2) Bromide_SpringCreekSpring.tsv - Bromide recovery concentrations observed in Spring Creek Spring (USGS site 385433114063901) discharge during a quantitative dual-tracer investigation in the Snake Creek drainage of Great Basin National Park, White Pine County, Nevada, October 2020 to March 2021. 3) Fluorescein_SpringCreekSpring.tsv - Fluorescein recovery concentrations observed in Spring Creek Spring (USGS site 385433114063901) discharge during a quantitative dual-tracer investigation in the Snake Creek drainage of Great Basin National Park, White Pine County, Nevada, September 2020 to February 2021.