Here we archive data collected during a nitrogen addition field experiment in the Sacramento River Deep Water Ship Channel. Calcium nitrate was added on 8 dates to a segment of the ship channel centered at Navigation Light 74. Prior to and following the nutrient additions, we collected water samples and sensor-based measurements at 7 sites between Navigation Light 70 and 76. Water samples for nutrient analyses were collected in 500 mL High Density Poly Ethylene (HDPE) amber containers from 1 m and 8 m depths using a peristaltic pump. Whole water samples for phytoplankton analysis were collected from 1 m depth in 500 mL clear HDPE containers and immediately preserved with 20 mL of Lugol’s iodine solution (Lugol’s, ENG Scientific, Inc.). We collected a vertically integrated zooplankton sample by lowering a 163 µM net to 8 m (~1 m off the bottom) and raised it using an electric winch. We transferred seston contents into new 250 mL HDPE containers and immediately preserved them with 10 mL of Lugol’s. A 1-mL unfiltered water sample from the surface was collected for bacterioplankton abundance. Vertical profiles of temperature, conductivity, dissolved oxygen, pH, turbidity, and algal fluorescence were made using a YSI EXO2 sonde. Measurements were made at 0.5 m and at 1 m resolution to the bottom. The sensor was allowed to stabilize at each depth for at least 10 seconds prior to data acquisition. In total, we sampled on 27 dates between July 8 and August 26, 2019. Lastly, we also report data used in metabolism calculations. Data include 3 methods for estimating metabolism based on continuous DO measurements, laboratory incubations, and an isotope model. Ancillary data include light extinction, stratification strength, wind speed, and gas transfer velocity, Metabolism measurements range from July 2 to September 18, 2019.